A New ent-Kaurane type Diterpenoid Glycoside from Inula japonica Thunb

Jiang Jiang Qin,Jia Xian Zhu,Wei Dong Zhang1,2,Yan Zhu,Jian Jun Fu,Xiao Hua Liu,Hui Zi Jin 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.10

A new ent-kaurane type diterpenoid glycoside, 17-O-β-D-glucopyranosyl-16α-ent-kauran-19-oic acid (1), together with 17-hydroxy-16α-ent-kauran-19-oic acid (2), 16α,17-dihydroxyl-ent-kauran-19-oic acid (3), and 16α-hydroxy-17-acetoxy-ent- kauran-19-oic acid (4) were isolated from the aerial parts of Inula japonica Thunb. The structure of 1 was determined mainly by use of 1D and 2D NMR spectroscopic techniques including HSQC, 1H-1H COSY, HMBC, and NOESY. In addition, 4 exhibited significant inhibitory activity on NO production in LPS-stimulated RAW264.7 cells with IC50 value of 14.3 μg/mL.

Anomalous magnetization jumps in granular Pb superconducting films

Zhang An-Lei,Jiang Wan-Yan,Chen Xing-Hong,Zhang Xiao-Ke,Lu Wen-Lai,Chen Fei,Feng Zhen-Jie,Cao Shi-Xun,Zhang Jin-Cang,Ge Jun-Yi 한국물리학회 2022 Current Applied Physics Vol.35 No.-

In granular superconductors, the grain boundaries are closely related to the vortex dynamics and the macroscopic superconducting properties. In our research, Pb films with different grain sizes were prepared by tuning the substrate temperature. With the grain size decreasing, Pb films are prone to feature the anomalous magnetization jumps in the M − T curves, while in the M − H curves flux avalanche happens. Both phenomena appear in the same region of the H − T phase diagram and thus are considered to have the same origin. The further theoretical analysis shows that with grain size decreasing the pinning mechanism evolves from a mixed δTc and δl pinning to the δl pinning mechanism. The results shed light on the study of pinning mechanism for granular superconductors and is beneficial to the potential application of manipulating vortex pinning by regulation of intrinsic defects.

Identification and Functional Analysis of the Chain Length Determinant Gene ste8 Involved in the Biosynthesis of Ebosin by Streptomyces sp.139

( Zhang Yang ),( Xiao Hua Li ),( Xiao Qaing Qi ),( Jun Jie Shan ),( Rong Jiang ),( Lian Hong Guo ),( Ren Zhang ),( Yuan Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.11

Ebosin, a novel exopolysaccharide produced by Streptomyces sp. 139, has obvious antirheumatic arthritis activity in vivo, and its biosynthesis gene cluster (ste), consisting of 27 open reading frames, has been identified. This paper reports our study of the gene functionality of ste8, the predicted protein product of which is homologous to some bacterial chain length determinant Wzz proteins. For characterization of Ste8, ste8 was cloned and expressed in the mutant strain E. coli 086:H2 (Δwzz). The functional complementation of wzz by ste8 was demonstrated by the restoration of wild-type lipopolysaccharide biosynthesis and increased levels of serum resistance of E. coli 086:H2 (Δwzz) (pET30a-ste8). To examine the function of ste8 in ebosin biosynthesis, the gene was knocked out with a double crossover via homologous recombination. The molecular weight of the ebosin derivative EPS-8m produced by the mutant Streptomyces sp. 139 (ste8-) was much lower than that of ebosin, and the binding activity of EPS-8m for IL-1R decreased significantly compared with ebosin. These results demonstrate that ste8 encodes a chain length determinant (Wzz) that functions in ebosin biosynthesis.

Anti-inflammatory Activities of Lupane-triterpenoids In Vitro and Their Phytochemical Fingerprinting from Leaves of Acanthopanax gracilistylus

Xiao Jun Li,Ling Dai,Zhi Li,Xiao Dan Zhang,Xiang Qian Liu,Qin Peng Zou,Xia Xie 한국생약학회 2015 Natural Product Sciences Vol.21 No.2

The activities on the inhibition of NO on LPS-induced RAW 264.7 macrophages were investigated in this work. A simple and sensitive method has been developed and validated for fingerprinting analysis of leaves of Acanthopanax gracilistylus W.W. Smith (AGS). The cytotoxicity and inhibition of NO on LPS-induced RAW 264.7 cells of the extract and triterpenoids were determined. Optimal conditions of HPLC analysis were established as follows. The separation was performed with an ODS-C18 column at 30 oC, the detected wavelength was 210 nm, the flow rate was 1 mL/min, and the mobile phase consisted of acetonitrile (0.05% phosphoric acid) -0.05% phosphoric acid solution with gradient elution. Our results showed that impressic acid and acankoreaogenin was more effective on the inhibition of NO than the methanol extract and other compounds. There were seventeen peaks coexisted with similarities above 0.95 and nine lupane-triterpenoids including acankoreaogenin and impressic acid detected and identified. The result of anti-inflammatory activities provides a potential explanation for the use of AGS leaves as a herbal medicine in the treatment of inflammatory diseases. Our results also show that acankoreanogenin and impressic acid may be potentially useful in developing new anti-inflammatory agents. In addition, the fingerprint chromatography clearly illustrated and confirmed the material basis for the anti-inflammatory activities of this plant.

Hashing via Efficient Addictive Kernel for Logistics Image Classification

Xiao-jun Liu,Qiu-ling Li,Bin Zhang,Jun-yi Li 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.2

In this paper, fast image search with efficient additive kernels and kernel locality-sensitive hashing has been proposed. As to hold the kernel functions, recent work has probed methods to create locality-sensitive hashing , which guarantee our approach’s linear time, however existing methods still do not solve the problem of locality-sensitive hashing (LSH) and indirectly sacrifice the loss in accuracy of search results in order to allow fast queries. To improve the search accuracy, we show how to apply explicit feature maps into the homogeneous kernels, which help in feature transformation and combine it with kernel locality-sensitive hashing. We prove our method on several large datasets, and illustrate that it improve the accuracy relative to commonly used methods and make the task of object classification, content-based retrieval more fast and accurate.

Reliability Analysis for Serviceability Limit State of Pile Groups Foundation

Xiao-ya Bian,Jun-jie Zheng,Rong-Jun Zhang,Zhi-jun Xu 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.1

The aim of this paper is to present an approach for the assessment of the Serviceability Limit State (SLS) reliability of pile groups foundation combining the Ultimate Limit State (ULS) reliability method of pile groups. To this end, the linear relationship between the ULS and SLS reliability indices is presented using the formulas for calculating the ULS and SLS reliability indices of single piles. Meanwhile, the influence of system effects, correlation coefficient between pile group efficiency and system effects (ρζ,χ), and limiting tolerable settlement (Slt) on the SLS reliability analysis of pile groups foundation is studied using pile load test data compiled for illustration. The results indicate that both the ULS reliability index (βG-uls) and the SLS reliability index (βG-sls) of pile groups foundation decrease with ρζ,χ, that powerful system effects can enhance the ULS and SLS reliability of pile groups foundation, and that βG-sls increases with Slt. the reliability level estimated by pile load test data is equivalent to that incorporating the SLS requirements with a certain value of Slt between 15 mm and 25 mm. For Slt larger than this value, Reliability-based Design (RBD) of pile groups foundation will depend on the ULS requirements, but, conversely, RBD will depend on the SLS requirements.

Reduction of the contact resistance in copper phthalocyanine thin film transistor with UV/ozone treated Au electrodes

Jun Li,Liang Zhang,Xiao-Wen Zhang,Hao Zhang,Xue-Yin Jiang,Dong-bin Yu,Wen-Qing Zhu,Zhi-Lin Zhang 한국물리학회 2010 Current Applied Physics Vol.10 No.5

Bottom-contact (BC) copper phthalocyanine (CuPc) thin film transistor with UV/ozone treated Au as a source/drain electrode was fabricated and the contact resistance was estimated from the transmission line method (TLM). Comparing the properties of OTFT with untreated Au electrode, the performance of the BC CuPc-TFT with the UV/ozone treated Au electrodes was significantly improved: saturation mobility increased from 4.69 × 10−3 to 2.37 × 10−2 ㎠/V s, threshold voltage reduced from −29.1 to −6.4 V, and threshold swing varied from 5.08 to 2.25 V/decade. The contact resistance of the device with UV/ozone treated Au electrodes was nearly 20 times smaller than that of the device with untreated Au electrodes at the gate voltage of −20 V. This result indicated that using the UV/ozone treated Au electrode is an effective method to reduce the contact resistance. The present BC configuration with UV/ozone treated Au electrodes could be a significant step towards the commercialization of OTFT technology.

Weighted FP-Tree Mining Algorithms for Conversion Time Data Flow

Xiao-jun Chen,Jia Ke,Qian-qian Zhang,Xin-ping Song,Xiao-ming Jiang 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.1

The data distribution in the data streams usually changes dynamically with time. Traditional mining algorithms based on transaction are difficult to establish the correlation between time characteristics and relationship features, thus making the results inaccurate. By analyzing the problems in the processing of time data stream, we put forward the concept of time gap degrees and design a mining algorithms based on weighted FP-Tree. We introduce the concept of FP-Tree node weights to transform the time data dynamically and excavate the data stream association rules. The experiments performed on the actual data set show that the algorithm can improve the recall and precision while consumes comparable computational time.

Elucidations of weak microcavity effect and improved pixel contrast ratio in Si-based top-emitting organic light-emitting diode

Xiao-Wen Zhang,Hua-Ping Lin,Jun Li,Fan Zhou,Bin Wei,Xue-Yin Jiang,Zhi-Lin Zhang 한국물리학회 2012 Current Applied Physics Vol.12 No.5

Microcavity effect and pixel contrast ratio (PCR) are key issues for top-emitting organic light-emitting diode (TOLED). Si and Ag are two widely used reflective anodes for constructing effective TOLEDs. The mechanism contributing to microcavity effect and PCR has been clarified by using conventional optoelectronic theory. Consequently, the differences of microcavity effect and PCR between Si-based and Agbased TOLEDs are discovered. Our results indicate that the Si-based TOLED possesses weak microcavity effect and improved PCR in comparison with Ag-based device. This is resulted from the low reflectivity and less reflective phase change of Si. The weak microcavity effect in Si-based TOLED also contributes to negligible variation of electroluminescent spectra with viewing angles and low device efficiency. Furthermore, Si-based TOLED shows rather high PCR which is about quintuple that of Ag-based device. Microcavity effect and pixel contrast ratio (PCR) are key issues for top-emitting organic light-emitting diode (TOLED). Si and Ag are two widely used reflective anodes for constructing effective TOLEDs. The mechanism contributing to microcavity effect and PCR has been clarified by using conventional optoelectronic theory. Consequently, the differences of microcavity effect and PCR between Si-based and Agbased TOLEDs are discovered. Our results indicate that the Si-based TOLED possesses weak microcavity effect and improved PCR in comparison with Ag-based device. This is resulted from the low reflectivity and less reflective phase change of Si. The weak microcavity effect in Si-based TOLED also contributes to negligible variation of electroluminescent spectra with viewing angles and low device efficiency. Furthermore, Si-based TOLED shows rather high PCR which is about quintuple that of Ag-based device.

ODV-Associated Proteins of the <i>Pieris rapae</i> Granulovirus

Wang, Xiao-Feng,Zhang, Bao-Qin,Xu, Hai-Jun,Cui, Ying-Jun,Xu, Yi-Peng,Zhang, Min-Juan,Han, Yeon Soo,Lee, Yong Seok,Bao, Yan-Yuan,Zhang, Chuan-Xi American Chemical Society 2011 JOURNAL OF PROTEOME RESEARCH Vol.10 No.6

<P><I>Alphabaculovirus</I> (lepidopteran-specific nucleopolyhedroviruses, NPV) and <I>Betabaculovirus</I> (granuloviruses, GV) are two main genera of the family Baculoviridae. The virion proteomes of <I>Alphabaculovirus</I> have been well studied; however, the <I>Betabaculovirus</I> virion compositions remain unclear. <I>Pieris rapae</I> granulovirus (PrGV) can kill larvae of <I>P. rapae</I>, a worldwide and important pest of mustard family crops. In this study, the occlusion-derived virus (ODV)-associated proteins of PrGV were identified using three mass spectrometry (MS) approaches. The MS analyses demonstrated that 47 proteins were present in PrGV-ODV. Of the 47 PrGV-ODV proteins, 33 have homologues identified previously in other baculovirus ODV/BVs, whereas 14 (P10, Pr21, Pr29, Pr35, Pr42, Pr54, P45/48, Pr83, Pr84, Pr89, Pr92, Pr111, Pr114 and FGF3) were newly identified ODV proteins. Seven of the 14 newly identified ODV proteins are specific to <I>Betabaculovirus</I>, including Pr35, Pr42, Pr54, Pr83, Pr84, Pr111 and Pr114. Furthermore, the data derived from these MS approaches were validated by immunoblotting analysis using antisera prepared from 11 randomly selected recombinant PrGV-ODV proteins (including 5 <I>Betabaculovirus</I>-unique proteins). Comparison analyses revealed the similar and different compositions between <I>Betabaculovirus</I> and <I>Alphabaculovirus</I> virions, which deepen our understanding of the baculovirus virion structure and provide helpful information on <I>Betabaculovirus</I>–host interaction studies.</P><P>We used three mass spectrometry (MS) approaches to identify the occlusion-derived virus (ODV)-associated proteins of the <I>Pieris rapae</I> Granulovirus. A total of 47 proteins were identified; 14 of them were first identified in the ODV, and 7 are specific to Granulovirus.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jprobs/2011/jprobs.2011.10.issue-6/pr2000804/production/images/medium/pr-2011-000804_0002.gif'></P>