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Xie Zhongyu,Yu Wenhui,Ye Guiwen,Li Jinteng,Zheng Guan,Liu Wenjie,Lin Jiajie,Su Zepeng,Che Yunshu,Ye Feng,Zhang Zhaoqiang,Wang Peng,Wu Yanfeng,Shen Huiyong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Mesenchymal stem cells (MSCs) are a common kind of multipotent cell in vivo, but their heterogeneity limits their further applications. To identify MSC subpopulations and clarify their relationships, we performed cell mapping of bone-marrow-derived MSCs through single-cell RNA (scRNA) sequencing. In our study, three main subpopulations, namely, the stemness subpopulation, functional subpopulation, and proliferative subpopulation, were identified using marker genes and further bioinformatic analyses. Developmental trajectory analysis showed that the stemness subpopulation was the root and then became either the functional subpopulation or the proliferative subpopulation. The functional subpopulation showed stronger immunoregulatory and osteogenic differentiation abilities but lower proliferation and adipogenic differentiation. MSCs at different passages or isolated from different donors exhibited distinct cell mapping profiles, which accounted for their corresponding different functions. This study provides new insight into the biological features and clinical use of MSCs at the single-cell level, which may contribute to expanding their application in the clinic.
Ye Guiwen,Li Jinteng,Yu Wenhui,Xie Zhongyu,Zheng Guan,Liu Wenjie,Wang Shan,Cao Qian,Lin Jiajie,Su Zepeng,Li Dateng,Che Yunshu,Fan Shuai,Wang Peng,Wu Yanfeng,Shen Huiyong 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Improving health and delaying aging is the focus of medical research. Previous studies have shown that mesenchymal stem cell (MSC) senescence is closely related to organic aging and the development of aging-related diseases such as osteoarthritis (OA). m6A is a common RNA modification that plays an important role in regulating cell biological functions, and ALKBH5 is one of the key m6A demethylases. However, the role of m6A and ALKBH5 in MSC senescence is still unclear. Here, we found that the m6A level was enhanced and ALKBH5 expression was decreased in aging MSCs induced by multiple replications, H2O2 stimulation or UV irradiation. Downregulation of ALKBH5 expression facilitated MSC senescence by enhancing the stability of CYP1B1 mRNA and inducing mitochondrial dysfunction. In addition, IGF2BP1 was identified as the m6A reader restraining the degradation of m6A-modified CYP1B1 mRNA. Furthermore, Alkbh5 knockout in MSCs aggravated spontaneous OA in mice, and overexpression of Alkbh5 improved the efficacy of MSCs in OA. Overall, this study revealed a novel mechanism of m6A in MSC senescence and identified promising targets to protect against aging and OA.
김희성(Hee-Sung Kim),박인호(In-Ho Park),왕문혜(Wenhui Wang),황의균(Eui-Kyun Hwang),이민수(Min-Su Lee),이길재(Gil-Jae Lee) 한국콘텐츠학회 2022 한국콘텐츠학회논문지 Vol.22 No.3
본 연구의 목적은 대학생의 대학몰입에 영향을 미치는 개인 및 대학 교육 변인을 탐색하는 것이다. 이를 위해 한국교육개발원의 ‘한국교육종단연구(KELS: Korea Educational Longitudinal Survey) 2005’에서 수집된 11차(2018) 데이터를 위계적 회귀분석(Hierarchical regression)을 활용하여 분석한다. 본 연구의 종속변수는 Tinto 모델을 기준으로 학업몰입, 사회적 몰입, 이를 통합한 대학몰입으로 구성하였다. 대학생의 대학몰입에 영향을 미치는 개인 및 대학 수준 변인을 확인한 결과, 대학생활 변인인 학습 과정, 학업 태만, 대학풍토, 교수와의 교류, 친구와의 교류가 유의미한 영향을 미치는 것으로 분석되었다. 학생의 개인배경 중 성별이 학업몰입과 대학몰입에 영향을 미치는 것으로 분석되었다. 대학생활 변인을 추가한 모델에서는 영향을 미치지 않았지만, 개인배경과 대학배경을 투입한 모델에서는 모두 여학생이 학업몰입과 대학몰입에 낮은 것으로 나타났다. 개인배경 중 사회적 몰입에 영향을 미치는 요인으로 전공계열이 유의미한 것으로 분석되었는데 의약 계열의 사회적 몰입이 높은 것으로 분석되었다. 이러한 분석 결과를 토대로 정책적 시사점이 논의되었다. The purpose of this study is to analyze the variables affecting the institutional commitment of college students. Based on the relevant literature review, this study subdivided institutional commitment of college students into three; academic integration, social integration and institutional commitment. To achieve the goals, the hierarchical regression analysis was conducted using KELS(Korea Educational Longitudinal Survey) data collected by KEDI in 2018. Major findings are as follows: factors related to college experiences such as learning styles, negligence of learning, college climate, interaction with faculty members or peer group were found to be associated with the institutional commitment of college students. With regard to students’ background, male students revealed lower level of academic integration and institutional commitment. The regression model disclosed that students from medicine demonstrated higher social integration compared to other majors. Based on the findings of the study, policy implications were discussed.