Down-regulation of SENP1 Expression Increases Apoptosis of Burkitt Lymphoma Cells

Huang, Bin-Bin,Gao, Qing-Mei,Liang, Wei,Xiu, Bing,Zhang, Wen-Jun,Liang, Ai-Bin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: To investigate the effect of down-regulation of Sentrin/SUMO-specific protease 1 (SENP1) expression on the apoptosis of human Burkitt lymphoma cells (Daudi cells) and potential mechanisms. Methods: Short hairpin RNA (shRNA) targeting SENP1 was designed and synthesized and then cloned into a lentiviral vector. A lentiviral packaging plasmid was used to transfect Daudi cells (sh-SENP1-Daudi group). Daudi cells without transfection (Daudi group) and Daudi cells transfected with blank plasmid (sh-NC-Daudi group) served as control groups. Flow cytometry was performed to screen GFP positive cells and semiquantitative PCR and Western blot assays were employed to detect the inference efficiency. The morphology of cells was observed under a microscope before and after transfection. Fluorescence quantitative PCR and Western blot assays were conducted to measure the mRNA and protein expression of apoptosis related molecules (caspase-3, 8 and 9). After treatment with $COCl_2$ for 24 h, the mRNA and protein expression of hypoxia inducible factor -$1{\alpha}$ (HIF-$1{\alpha}$) was determined. Results: Sequencing showed the expression vectors of shRNA targeting SENP1 to be successfully constructed. Following screening of GFP positive cells by FCM, semiqualitative PCR showed the interference efficiency was $79.2{\pm}0.026%$. At 48 h after transfection, the Daudi cells became shrunken, had irregular edges and presented apoptotic bodies. Western blot assay revealed increase in expression of caspase-3, 8 and 9 with prolongation of transfection (P<0.05). Following hypoxia treatment, mRNA expression of HIF-$1{\alpha}$ remained unchanged in three groups (P>0.05) but the protein expression of HIF-$1{\alpha}$ markedly increased (P<0.05). However, in the sh-SENP1-Daudi group, the protein expression of HIF-$1{\alpha}$ remained unchanged Conclusion: SENP1-shRNA can efficiently inhibit SENP1 expression in Daudi cells. SENP1 inhibition may promote cell apoptosis. These findings suggest that SENP1 may serve as an important target in the gene therapy of Burkitts lymphoma.

White Spot Syndrome Virus Disease of Shrimp and Diagnostic Methods

Wen-Bin Zhan 한국수산과학회 양식분과 2002 韓國養殖學會誌 Vol.15 No.1

웹 기반 수준진단 시스템을 통한 맞춤형 생산정보화 도입 가이드라인에 관한 연구

Wen-Bin Zhao,이주연(Ju Yeon Lee),노상도(Sang Do Noh),조현제(Hyun Jei Jo),조용주(Yong Ju Jo),최석우(Seog Ou Choi) (사)한국CDE학회 2011 한국 CAD/CAM 학회 학술발표회 논문집 Vol.2011 No.1

제조기업이 생산정보화시스템(MES) 도입 시, 기업별로 생산환경의 특성이 모두 반영된 정보화로 추진되어야 하는 관계로 대기업과는 달리 중소기업에서 도입하는 데에 많은 어려움을 겪고 있다. 일반적으로 국내의 중소형 제조기업에서 MES 를 도입할 때 기업의 생산환경 특성을 반영하는 업종이나 규모, 생산방식 및 주문방식 등 요소를 고려하지 않고 획일적인 전략을 기반으로 도입을 하고 있으며, 이로 인해 도입 후에도 시스템이 제대로 활용되지 못하고 일부의 간단한 기능만을 사용하게 되거나, 이로 인한 사용자의 만족도가 낮아지는 등의 많은 문제점이 발생하고 있다. 본 논문에서는 생산정보화시스템(MES)의 효율적인 도입을 위해 웹 기반의 정보화 수준 진단시스템을 이용한 생산정보화의 보급/확산 전략을 도출한다. 또한 웹 포털 상에서 이해하기 쉽고 간단한 대화형식의 질문을 통해 해당 기업의 수준과 필요를 도출하여 맞춤형 생산정보화시스템(MES) 도입 가이드라인을 제시한다.

Dynamic Analysis and Design Calculation Methods for Powertrain Mounting Systems

Wen-Bin Shangguan,Zhao Yinyan 한국자동차공학회 2007 International journal of automotive technology Vol.8 No.6

A method for dynamic analysis and design calculation of a Powertrain Mounting System (PMS) including Hydraulic Engine Mounts (HEM) is developed with the aim of controlling powertrain motion and reducing low-frequency vibration in pitch and bounce modes. Here the pitch mode of the powertrain is defined as the mode rotating around the crankshaft of an engine for a transversely mounted powertrain. The powertrain is modeled as a rigid body connected to rigid ground by rubber mounts and/or HEMs. A mount is simplified as a three-dimensional spring with damping elements in its Local Coordinate System (LCS). The relation between force and displacement of each mount in its LCS is usually nonlinear and is simplified as piecewise linear in five ranges in this paper. An equation for estimating displacements of the powertrain center of gravity (C.G.) under static or quasi-static load is developed using Newton’'s second law, and an iterative algorithm is presented to calculate the displacements. Also an equation for analyzing the dynamic response of the powertrain under ground and engine shake excitations is derived using Newton’'s second law. Formulae for calculating reaction forces and displacements at each mount are presented. A generic PMS with four rubber mounts or two rubber mounts and two HEMs are used to validate the dynamic analysis and design calculation methods. Calculated displacements of the powertrain C.G. under static or quasi-static loads show that a powertrain motion can meet the displacement limits by properly selecting the stiffness and coordinates of the tuning points of each mount in its LCS using the calculation methods developed in this paper. Simulation results of the dynamic responses of a powertrain C.G. and the reaction forces at mounts demonstrate that resonance peaks can be reduced effectively with HEMs designed on the basis of the proposed methods.

Simulated Annealing Utilization for Intelligent Reflecting Surfaces

Wen-Bin Li,Thu L. N. Nguyen,Yoan Shin 한국통신학회 2021 한국통신학회 학술대회논문집 Vol.2021 No.6

In this paper, we first formulate the path loss model of an intelligent reflecting surface (IRS) in terms of IRS reflection coefficients, then adopt a simulated annealing algorithm to determine a global maximum point of the path loss. Consequently, we can improve the performance of IRS-assisted communication system by avoiding such position deviations in practice. Simulation results show that an optimal solution can be achieved by implementing the proposed algorithm.

Enhanced conversion efficiency of crystalline Si solar cells via luminescent down-shifting using Ba2SiO4 : Eu2+ phosphor

Wen Bin Hung,Juyn Ye Chen,Kien Wen Sung,Teng Ming Chen 한양대학교 세라믹연구소 2014 Journal of Ceramic Processing Research Vol.15 No.3

The colloidal submicron needle-like Ba2SiO4 : Eu2+ phosphor coated on microtextured silicon surface has been used as a luminescent down-shifting (LDS) material for enhancing the power conversion efficiency of solar cells. Under a simulated onesun illumination, upon phosphor coating the solar cells showed an enhancement of the short-circuit current density (Jsc) increased from 34.00 to 34.48 mA/cm2 , meanwhile the power conversion efficiency (PCE) was enhanced from 15.13% to 15.36%. The related underlying mechanism for these enhancements can be attributed to photon down-shifting and light scattering effect through the investigation of PLE spectrum, reflectance, and external quantum efficiency. Our results indicate that the colloidal submicron needle-like Ba2SiO4 : Eu2+ phosphor not only act as LDS centers in the UV region but also serve as an antireflection coating for enhancing the light absorption in the visible regime.

Differential antioxidation activities in two alfalfa cultivars under chilling stress

Wen-Bin Wang,김윤희,Haeng-Soon Lee,Xi-Ping Deng,Sang-Soo Kwak 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.4

To understand the adaptability of alfalfa (Medicago sativa L.) to chilling stress, we analyzed the antioxidative mechanism during seed germination. The germination rates of six alfalfa cultivars were studied comparatively at 10C. Xinmu No. 1 and Northstar were selected as chilling stress-tolerant and stress-sensitive cultivars for further characterization. After chilling treatment, Xinmu No. 1 showed higher seedling growth than Northstar. Xinmu No. 1 exhibited low levels of hydrogen peroxide and lipid peroxidation compared with Northstar. In addition, shoots in Xinmu No. 1 treated with chilling showed higher activities of the superoxide dismutase, ascorbate peroxidase (APX), and catalase than those of Northstar, whereas Xinmu No. 1 showed higher APX activity in roots that Northstar. These results indicated that high antioxidation activity in Xinmu No. 1 under chilling stress is well associated with tolerance to chilling condition during germination.

Continuous Conversion of Rice Starch Hydrolysate to 2-keto-D-gluconic Acid by Arthrobacter globiformis C224

Wen-Hua Teng,Wen-Jing Sun,Bin Yu,Fengjie Cui,Jing-Ya Qian,Jingze Liu,Liang Wang,Xiang-Hui Qi,Hua Wei 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.4

A continuous conversion process of rice starch hydrolysate to 2-keto-D-gluconic acid (2KGA) by Arthrobacter globiformis C224 was developed. Its feasibility for industrial application was also evaluated. Results showed that the initial cell concentration exceeding 1.25 g/L met the continuous 2KGA production at a stable dilution rate and media composition, while the dilution rate and feeding glucose concentration had a significant effect on 2KGA production performance. The optimal operating parameters were obtained as: 0.090/h of dilution rate and 171.0 g/L of feeding glucose concentration. Under these conditions, the steady state had a produced 2KGA concentration of 124.74 g/L, average volumetric productivity of 11.23 g/L/h,and yield of 0.97 g/g. In conclusion, continuous 2KGA production by the A. globiformis C224 strain would be a superior industrial process for the production of 2KGA in terms of its high 2KGA productivity and yield.

Research on the Correlation between Wavy Modeling of Garment and Fabric Properties

( Wen Bin Zhang ),( Wu Tuo ),( Jing Ru Liu ) 한국패션비즈니스학회 2004 패션 비즈니스 Vol.8 No.6

On the basis of garment spatial configuration technology, the correlation of fabric properties and garment modeling was studied in this paper. With abundant experimental data, practical modeling and the judgment of fabric properties and physical shape, we analyzed the change rules that restricting all kinds of parameters of fabric properties and garment modeling, and discussed the relation of wavy modeling and all correlated factors of fabric properties. The result showed the basis of choosing fabric for different style garment, which would help designers choosing fabric for modeling design or structure design.

Use Chains to Block DNS Attacks: A Trusty Blockchain-based Domain Name System

Wen-Bin Hsieh,Jenq-Shiou Leu,Jun-Ichi Takada 한국통신학회 2022 Journal of communications and networks Vol.24 No.3

The Internet has become one of the most importanttechnologies in the world, and hackers use various methods tolaunch cyber attacks to profit from it. Phishing is one of famoussocial engineering attacks, it is often used to steal user data,including login credentials and credit card numbers. Althoughthe Transport Layer Security certificate is used to verify the trustof websites, there are still a series of vulnerabilities. The demandfor trusted IP addresses has led a lot of research, including IPwhitelisting, DNS filtering and so on. However, these technologiesstill have many shortcomings. In view of this, we proposeda novel mechanism for verifying websites using blockchaintechnology. The URL and IP address of a permissioned websiteare recorded in blockchain through a specific smart contract. A DNS query is executed through a smart contract designedto avoid URL redirection attacks. With the help of immutablenature of blockchian, phishing websites can be detected. Themechanism will not add any load to users and provides tamperprooffunctions based on the characteristics of blockchain. Thecomparison of related works shows that the proposed mechanismis more secure. We also provided a reference implementationof the proposed mechanism on Ethereum Quorum simulationplatform, which proves the effectiveness and practicability of themechanism.