miR-582-5p inhibits migration and chemo-resistant capabilities of colorectal cancer cells by targeting TNKS2

Xiao Weixing,Zhou Haijun,Chen Bingrong,Shen Bin,Zhou Jun 한국유전학회 2022 Genes & Genomics Vol.44 No.6

Background: Metastasis and chemo-resistance are still important factors that limit the overall efficacy of colorectal cancer treatment. Understanding the detailed molecular mechanism and identifying potential biomarkers are of great value in prognosis prediction and risk stratification. Objective: We investigated the role of miR-582-5p in colorectal cancer pathogenesis, progression and chemo-resistance. Furthermore, we explored the underlying molecular mechanism of miR-582-5p in modulation of malignant behaviors of colorectal cancer cells. Methods: Clinical samples and colorectal cancer cell lines were applied to explore miR-582-5p expression level and its significance on tumor cell metastasis and chemo-resistance. Transwell study and cellular survivability study were performed to explore the influences of miR-582-5p expression modulation on tumor cell chemo-resistance and invasion/migration. Dual-luciferase reporter gene assay was conducted to explore the influences of miR-582-5p on its target gene TNKS2. Results: Colorectal cancer patients with lymph node or distal organ metastatic diseases exhibited significantly lower level of miR-582-5p. In vitro studies have indicated that miR-582-5p inhibition significantly increased migration and chemo-resistant capabilities of tumor cells. And dual-luciferase reporter gene assay demonstrated that miR-582-5p exhibited its influences on the biological behavior of tumor cells by targeting TNKS2. Conclusions: Our study demonstrated for the first time that miR-582-5p played an important role for colorectal tumor cell metastasis and chemo-resistance. Our research also indicated that miR-582-5p and its target gene TNKS2 could be novel biomarkers for metastatic disease prediction, overall prognosis evaluation, as well as potential therapeutic target for colorectal cancer patients.

Pretreatment of sweet sorghum bagasse by alkaline hydrogen peroxide for enhancing ethanol production

Xudong Li,Weixing Cao,Chen Sun,Jiangping Qiu,Ronghou Liu,Le Zhang 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.3

Effects of severe and mild alkaline hydrogen peroxide (AHP) pretreatment on ethanol production from sweet sorghum bagasse via pre-simultaneous saccharification fermentation, and the chemical structure changes of the substrates were investigated. The results showed that the bagasse pretreated by severe AHP could produce more ethanol than that of mild AHP. The maximum ethanol concentration of the bagasses from mild and severe AHP pretreatment with 8% bagasse loading was 7.642±0.140 g/L and 19.330±0.085 g/L, respectively. Moreover, the FTIR and NMR analysis illustrated that the molecule and surface structures of the pretreated bagasse were significantly changed compared with the control. The potential biomass energy production of the effluent from the pretreatment was also briefly discussed for future utilization of waste solution.The heat energy potentials of waste solution with severe and mild AHP pretreatment were 367.2 kJ/Leffluent and 327.6 kJ/Leffluent, respectively.

Flow patterns of solid in water in oil (S/W/O) compound droplets formation in a microfluidic device with perpendicular shear

Dawei Pan,Qiang Chen,Lan Xu,Can Yang,Meifang Liu,Weixing Huang,Bo Li 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.75 No.-

Theflow patterns and corresponding breakup dynamics of solid in water in oil compound droplets underperpendicular shear force were experimentally investigated in a microfluidic device. Three typicalflowpatterns, namely double-encapsulation, plugs, and cobbles, were found and be quantitativelydistinguished by the capillary number (Cac) of continuous phase and theflow rate ratio (R) ofcontinuous phase to dispersed phase. It is found that, under all offlow regimes, the consecutive entry ofsolid particles leads d1 (the neck thickness along the center of the side channel) varying periodically. Inthe double-encapsulation regime, the neck thickness (d2) between thefirst and second particle increasesas the interface deformation recovers driven by the interfacial tension force, whereas the neck thickness(d3) between the second and third particle decreases to break off because the neck has been stretchedlong to become thin enough. As for the cobbles regime, hydrostatic pressure difference still remainsdominant, but not a shearing force. Specially, different from the W/O droplet formation, it suggests thatvarious breakup dynamics are always caused by a non-free deformation of S/W interface and the largediameter of solid particle. Finally, theflow pattern diagrams dependent on Cac and R are obtained.

Kinetic modeling for chromatographic separation of cytosine monophosphate and uracil monophosphate

Haibin Qu,Yong Chen,Weixing Dai,Xuesong Liu,Yiyu Cheng 한국화학공학회 2006 Korean Journal of Chemical Engineering Vol.23 No.5

pharmaceutical industries. In this study, chromatographic separation of the two nucleotides CMP and UMP was sim-ulated by the equilibrium-dispersive (ED) model, and the adsorption isotherms in the ED model were determined bythe inverse method. Prediction performance of the model was validated under three diferent kinds of conditions andthe importance of selecting isotherms was discussed in detail. Excellent agreement was achieved with the experi-mental band profiles and the prediction of the ED model. The ED model with bi-Langmuir isotherm was especiallysuitable for simulating chromatographic separation of CMP and UMP. The error of prediction by the ED model with

Renewable biomass derived hierarchically porous carbonaceous sponges and their magnetic nanocomposites for removal of organic molecules from water

Hongwei Zhou,Bo Yan,Jialiang Lai,Hanbin Liu,Aijie Ma,Weixing Chen,Xilang Jin,Weifeng Zhao,Gai Zhanga 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.58 No.-

This work describes the preparation, characterization and removal capability of a novel biomass derived carbonaceous sponges (CS) and their nanocomposites. The CS has hierarchically porous structure which is composed of lamellar structures and secondary porous structures. The pore size is on a scale from 1 nm to 200 μm. Utilizing the CS as adsorbents, rapid removal of model organic molecules, including methylene blue (MB), methyl orange (MO) and crystal violet (CV), from their aqueous solutions can be completed within 1 min with the assistance of pressure and the removal efficiency reaches up to 100%, 81% and 98%, respectively. The removal capabilities for CS towards MB, MO and CV are 0.0769 g/g, 0.2218 g/g and 1.0384 g/g, respectively and 0.0635 g/g, 0.0977 g/g and 0.8634 g/g, respectively for CS nanocomposites.

Dynamic Beehive Detection and Tracking System Based on YOLO V5 and Unmanned Aerial Vehicle

Gao Peng,이강빈,쿠스위디얀토 루카스 위쿠,유승화,Hu Kai,Liang Gaotian,Chen Yufeng,Wang Weixing,Liao Fei,정유석,전문석,최인찬,한웅철 한국농업기계학회 2022 바이오시스템공학 Vol.47 No.4

Purpose With urban development and improvements in human living conditions, wild beehives in densely populated areas present a threat to human safety. The traditional manual method of clear beehives may result in secondary injury to humans. Methods This paper proposes a beehive detection model based on YOLO V5 by introducing the Shufe Block V2 and depthwise separable convolution (DSC) modules to decrease the original model parameters. The model can be deployed on edge computation devices such as Raspberry 4B with good detection accuracy. The PID algorithm and dual servo motors were combined with the object detection model to track the beehive automatically. The results of experiments showed that the inference speed of the improved beehive detection model was 92.5% faster than the original YOLO V5s model, although the detection accuracy and other indicators were not signifcantly diferent. Results The accuracy of the system in this study was as high as 96% in real-time detection, and the maximum recognition distance was 2.5 m. The performance test results of the system deployed on an unmanned aerial vehicle (UAV) showed that 90% of the beehive tracking process could be completed within 2 s, positioning the object in the center of the images collected by the camera. At the same time, when the UAV was moving at random, the detection and tracking system could still follow the beehive quickly and automatically. Conclusion The detection model and tracking system established in this paper provide important support to reduce the secondary damage to the rescue workers that may occur in beehive governance.

Preparation and Self-Healing Behaviors of Poly(acrylic acid)/Cerium Ions Double Network Hydrogels

Hongwei Zhou,Guohe Xu,Jie Li,Shihao Zeng,Xiaolong Zhang,Zhaohui Zheng,Xiaobin Ding,Weixing Chen,Qiguan Wang,Wenzhi Zhang 한국고분자학회 2015 Macromolecular Research Vol.23 No.12

This work aims at developing an approach to poly(acrylic acid)/cerium ions (PAA/cerium ions) double network hydrogels and exploring the self-healing properties of the hydrogels, with expectation to provide some clues for constructing new healable gel actuators and enrich the family of self-healable hydrogels. The hydrogel is covalently crosslinked by a traditional crosslinking agent N,N'-methylene bis-acrylamide to form the first network and further physically crosslinked by the ionic interaction between cerium ions and the carboxyl groups in the hydrogel network. The preparation method and self-healing behaviors of the hydrogels are investigated.

Asphaltene thermal treatment and optimization of oxidation conditions of low-cost asphaltene-derived carbon fibers

Peiyuan Zuo,Desirée Leistenschneider,Yuna Kim,Zahra Abedi,Douglas G. Ivey,Xuehua Zhang,Weixing Chen 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.104 No.-

This investigation is aimed at determining the conditions for the oxidation/stabilization of asphaltenefibers through extensive physicochemical characterization and evaluation of mechanical properties ofthe resulting carbon fibers. Melt spinning was used to fabricate green fibers using asphaltenes, from bothstraight solvent-de-asphalting (SDA) processing and subsequent thermal treatment. Thermal pretreatmentof SDA asphaltenes was found to yield much improved carbon fiber properties. An oxidation treatmentof HT300-derived (thermal pretreatment at 300 C) carbon fibers provided the best mechanicalproperties with an average tensile strength of 1130 MPa and an average Young’s modulus of 71 GPa. Thermal treatment of SDA asphaltenes led to much lower sulfur content in both oxidized and carbonizedfibers when oxidation was performed at 300 C. BET, XPS, and XRD analyses showed that carbon fibersfabricated from treated asphaltenes with improved mechanical properties had a lower surface area, lowerpore volume, higher amount of stable functional groups than SDA asphaltene-derived carbon fibers.

Neoadjuvant Chemotherapy-Guided Bladder-Sparing Treatment for Muscle-Invasive Bladder Cancer: Results of a Pilot Phase II Study

Hongzhe Shi,Wen Zhang,Xingang Bi,Dong Wang,Zejun Xiao,Youyan Guan,Kaopeng Guan,Jun Tian,Hongsong Bai,Linjun Hu,Chuanzhen Cao,Weixing Jiang,Zhilong Hu,Jin Zhang,Yan Chen,Shan Zheng,Xiaoli Feng,Changlin 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4

Purpose Reduced quality of life after cystectomy has made bladder preservation a popular research topic for muscle-invasive bladder cancer (MIBC). Previous research has indicated significant tumor downstaging after neoadjuvant chemotherapy (NAC). However, maximal transurethral resection of bladder tumor (TURBT) was performed before NAC to define the pathology, impacting the real evaluation of NAC. This research aimed to assess real NAC efficacy without interference from TURBT and apply combined modality therapies guided by NAC efficacy. Materials and Methods Patients with cT2-4aN0M0 MIBC were confirmed by cystoscopic biopsy and imaging. NAC efficacy was assessed by imaging, urine cytology, and cystoscopy with multidisciplinary team discussion. Definite responders (≤ T1) underwent TURBT plus concurrent chemoradiotherapy. Incomplete responders underwent radical cystectomy or partial cystectomy if feasible. The primary endpoint was the bladder preservation rate. Results Fifty-nine patients were enrolled, and the median age was 63 years. Patients with cT3-4 accounted for 75%. The median number of NAC cycles was three. Definite responders were 52.5%. The complete response (CR) was 10.2%, and 59.3% of patients received bladder-sparing treatments. With a median follow-up of 44.6 months, the 3-year overall survival (OS) was 72.8%. Three-year OS and relapse-free survival were 88.4% and 60.0% in the bladder-sparing group but only 74.3% and 37.5% in the cystectomy group. The evaluations of preserved bladder function were satisfactory. Conclusion After stratifying MIBC patients by NAC efficacy, definite responders achieved a satisfactory bladder-sparing rate, prognosis, and bladder function. The CR rate reflected the real NAC efficacy for MIBC. This therapy is worth verifying through multicenter research.