5G-RAN Slicing by utilizing RL-based Approaches for V2X Service

WeiJian Zhou,Chaitali J. Pawase,KyungHi Chang 한국통신학회 2022 한국통신학회 학술대회논문집 Vol.2022 No.2

5G mobile systems host a variety of services. Therefore, the main challenge on designing 5G networks is how to address different types of users with diverse QoS requirements on a shared physical network infrastructure. Radio Access Network (RAN) slicing has been introduced as a solution to these challenges. The RAN slicing have a limitation to provide two common services for 5G, namely eMBB and Cellular V2X. We propose an RAN slicing strategy based on reinforcement learning (RL) to allocate radio resources for different slices while considering the resource utilization to maximize the efficiency.

A Deep Reinforcement Learning based 5G-RAN Slicing Strategy for V2X Services

WeiJian Zhou,Chaitali J. Pawase,KyungHi Chang 한국통신학회 2021 한국통신학회 학술대회논문집 Vol.2021 No.6

Vehicular communication is a key technology in intelligent transportation systems which links vehicles, roadside units, and pedestrians. One of the key factor for providing design flexibility is Slicing of the Radio Access Network (RAN) which enables 5G systems to support heterogeneous services on one platform through a custom slice for each service. In this regard, this paper provides an overview of an RAN slicing strategy based on Deep Reinforcement Learning (DRL) for a heterogeneous network with two generic 5G services, such as enhanced mobile broadband (eMBB) and vehicle-to-everything (V2X).

Real-time RL-based 5G Network Slicing Design and Traffic Model Distribution: Implementation for V2X and eMBB Services

WeiJian Zhou,Azharul Islam,KyungHi Chang 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.9

As 5G mobile systems carry multiple services and applications, numerous user, and application types with varying quality of service requirements inside a single physical network infrastructure are the primary problem in constructing 5G networks. Radio Access Network (RAN) slicing is introduced as a way to solve these challenges. This research focuses on optimizing RAN slices within a singular physical cell for vehicle-to-everything (V2X) and enhanced mobile broadband (eMBB) UEs, highlighting the importance of adept resource management and allocation for the evolving landscape of 5G services. We put forth two unique strategies: one being offline network slicing, also referred to as standard network slicing, and the other being Online reinforcement learning (RL) network slicing. Both strategies aim to maximize network efficiency by gathering network model characteristics and augmenting radio resources for eMBB and V2X UEs. When compared to traditional network slicing, RL network slicing shows greater performance in the allocation and utilization of UE resources. These steps are taken to adapt to fluctuating traffic loads using RL strategies, with the ultimate objective of bolstering the efficiency of generic 5G services.

YTHDF1 promotes the proliferation, migration, and invasion of prostate cancer cells by regulating TRIM44

Li Weijian,Chen Gaohuang,Feng Zhenyu,Zhu Baoyi,Zhou Lilin,Zhang Yuying,Mai Junyan,Jiang Chonghe,Zeng Jianwen 한국유전학회 2021 Genes & Genomics Vol.43 No.12

Background Prostate cancer (PCa) is one of the most common malignancies in men. YTHDF1 may play an important role in promoting PCa progression, but there is no reports to date on YTHDF1 function in PCa. Objective This study explored whether YTHDF1 could regulate TRIM44 in PCa cells. Methods By querying the TCGA database, we evaluated YTHDF1 expression in PCa, the OS and DFS of YTHDF1, and the correlation between YTHDF1 and TRIM44 in PCa. We constructed vectors to interfere with YTHDF1 expression and overexpress TRIM44 to examine the role of YTHDF1 and TRIM44 in PCa cells. Diferentially expressed mRNAs were identifed by mRNA sequencing. The levels of YTHDF1, TRIM44, LGR4, SGTA, DDX20, and FZD8 were measured by qRT-PCR and WB was used to determine YTHDF1 and TRIM44 expression. A CCK-8 assay was used to assess cell proliferation. A Transwell chamber assay was used measure cell migration and invasion ability. Results YTHDF1 was highly expressed in both Pca tissues and cells. PCa patient prognosis with high YTHDF1 expression was relatively poor. Cell function experiments showed that inhibiting YTHDF1 expression decreased cell proliferation, migration, and invasion. RNA sequencing analysis revealed that YTHDF1 may promote PCa cell proliferation, migration, and invasion by modulating TRIM44 expression. Cell function experiments further verifed that YTHDF1 promoted PCa cell proliferation, migration, and invasion by regulating TRIM44. Conclusions YTHDF1 enhances PCa cell proliferation, migration, and invasion by regulating TRIM44.

Antisense oligodeoxynucleotides against dynamin-related protein 1 reduce remifentanilinduced hyperalgesia by modulating spinal N-methyl-D-aspartate receptor expression in rats

Songyi Zhou,Yizhao Pan,Yan Zhang,Lijun Gu,Leikai Ma,Qingqing Xu,Weijian Wang,Jiehao Sun 대한통증학회 2023 The Korean Journal of Pain Vol.36 No.3

Background: Spinal N-methyl-D-aspartate (NMDA) receptor activation is attributed to remifentanil-induced hyperalgesia (RIH). However, the specific mechanism and subsequent treatment is still unknown. Previous studies have shown that the dynamin-related protein 1 (DRP1)-mitochondria-reactive oxygen species (ROS) pathway plays an important role in neuropathic pain. This study examined whether antisense oligodeoxynucleotides against DRP1 (AS-DRP1) could reverse RIH. Methods: The authors first measured changes in paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) at 24 hours before remifentanil infusion and 4, 8, 24, and 48 hours after infusion. The expression levels of DRP1 and NR2B were measured after behavioral testing using Western blotting. In addition, DRP1 expression was knocked down by intrathecal administration of AS-DRP1 to investigate the effects of DRP1 on RIH. The behavioral testing, the expression levels of spinal DRP1 and NR2B, and dorsal mitochondrial superoxide were measured. Changes in mitochondrial morphology were assessed using electron microscopy. Results: After remifentanil exposure, upregulation of spinal DRP1 and NR2B was observed along with a reduction in PWMT and PWTL. In addition, AS-DRP1 improved RIH-induced PWTL and PWMT (P < 0.001 and P < 0.001) and reduced remifentanil-mediated enhancement of spinal DRP1 and NR2B expression (P = 0.020 and P = 0.022). More importantly, AS-DRP1 reversed RIH-induced mitochondrial fission (P = 0.020) and mitochondrial superoxide upregulation (P = 0.031). Conclusions: These results indicate that AS-DRP1 could modulate NMDA receptor expression to prevent RIH through the DRP1-mitochondria-ROS pathway.

Parametric study on bearing capacity of CFST members considering the concrete horizontal casting effect

Wenbo Sun,Yiqun Luo,Weijian Zhou,Wei Huang 국제구조공학회 2012 Steel and Composite Structures, An International J Vol.13 No.3

Concrete filled steel tubular (CFST) member has been widely used in the construction of highrise buildings for its high axial bearing capacity. It can also be applied on long-span structures such as spatial structures or bridges not only for its high bearing capacity but also for its construction convenience. Concrete casting effect of CFST member is considered in the study of its bearing capacity in this paper. Firstly, in order to authenticate the applicability of constitutive relationship and yield criterion of steel and concrete based on FEM, two ANSYS models are built to simulate and compared with other’s test. Secondly, in order to find the huge difference in bearing capacity due to different construction processes, two full-size CFST models are studied when they are horizontally cast and axially compressed. Finally, the effects of slenderness ratio (L/D) and confining parameter (D/t) of CFST members are studied to reveal the intrinsic links between bearing capacity and slenderness ratio or confining parameter.

Antimicrobial and Cytotoxic Activity of Endophytic Fungi from Lagopsis supina

Zhang Dekui,Sun Weijian,Xu Wenjie,Ji Changbo,Zhou Yang,Sun Jingyi,Tian Yutong,Li Yanling,Zhao Fengchun,Tian Yuan 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.4

In this study, five endophytic fungi belonging to the Aspergillus and Alternaria genera were isolated from Lagopsis supina. The antimicrobial activity of all fungal fermented extracts against Staphylococcus and Fusarium graminearum was tested using the cup-plate method. Among them, Aspergillus ochraceus XZC-1 showed the best activity and was subsequently selected for large-scale fermentation and bioactivity-directed separation of the secondary metabolites. Four compounds, including 2- methoxy-6-methyl-1,4-benzoquinone (1), 3,5-dihydroxytoluene (2), oleic acid (3), and penicillic acid (4) were discovered. Here, compounds 1 and 4 displayed anti-fungal activity against F. graminearum, F. oxysporum, F. moniliforme, F. stratum, Botrytis cinerea, Magnaporthe oryzae, and Verticillium dahliae with diverse MIC values (128–512 μg/ml), which were close to that of the positive control antifungal, actidione (64–128 μg/ml). Additionally, compounds 1 and 4 also exhibited moderate antibacterial activity against S. aureus, Listeria monocytogenes, Escherichia coli, and Salmonella enterica, with low MIC values (8–64 μg/ml). Moreover, compounds 1 and 4 displayed selective cytotoxicity against cancer cell lines as compared with the normal fibroblast cells. Therefore, this study proposes that the endophytic fungi from L. supina can potentially produce bioactive molecules to be used as lead compounds in drugs or agricultural antibiotics.

Dexmedetomidine alleviates blood-brain barrier disruption in rats after cerebral ischemia-reperfusion by suppressing JNK and p38 MAPK signaling

Zhu Canmin,Wang Dili,Chang Chang,Liu Aofei,Zhou Ji,Yang Ting,Jiang Yuanfeng,Li Xia,Jiang Weijian 대한약리학회 2024 The Korean Journal of Physiology & Pharmacology Vol.28 No.3

Dexmedetomidine displays multiple mechanisms of neuroprotection in ameliorating ischemic brain injury. In this study, we explored the beneficial effects of dexmedetomidine on blood-brain barrier (BBB) integrity and neuroinflammation in cerebral ischemia/reperfusion injury. Sprague-Dawley rats were subjected to middle cerebral artery occlusion (MCAO) for 1.5 h and reperfusion for 24 h to establish a rat model of cerebral ischemia/reperfusion injury. Dexmedetomidine (9 g/kg) was administered to rats 30 min after MCAO through intravenous injection, and SB203580 (a p38 MAPK inhibitor, 200 g/kg) was injected intraperitoneally 30 min before MCAO. Brain damages were evaluated by 2,3,5-triphenyltetrazolium chloride staining, hematoxylin-eosin staining, Nissl staining, and brain water content assessment. BBB permeability was examined by Evans blue staining. Expression levels of claudin-5, zonula occludens-1, occludin, and matrix metalloproteinase-9 (MMP-9) as well as M1/M2 phenotypes-associated markers were assessed using immunofluorescence, RT-qPCR, Western blotting, and gelatin zymography. Enzyme-linked immunosorbent assay was used to examine inflammatory cytokine levels. We found that dexmedetomidine or SB203580 attenuated infarct volume, brain edema, BBB permeability, and neuroinflammation, and promoted M2 microglial polarization after cerebral ischemia/reperfusion injury. Increased MMP-9 activity by ischemia/reperfusion injury was inhibited by dexmedetomidine or SB203580. Dexmedetomidine inhibited the activation of the ERK, JNK, and p38 MAPK pathways. Moreover, activation of JNK or p38 MAPK reversed the protective effects of dexmedetomidine against ischemic brain injury. Overall, dexmedetomidine ameliorated brain injury by alleviating BBB permeability and promoting M2 polarization in experimental cerebral ischemia/reperfusion injury model by inhibiting the activation of JNK and p38 MAPK pathways.