In Vitro Evaluation of Swine-Derived Lactobacillus reuteri: Probiotic Properties and Effects on Intestinal Porcine Epithelial Cells Challenged with Enterotoxigenic Escherichia coli K88

( Zhilin Wang ),( Li Wang ),( Zhuang Chen ),( Xianyong Ma ),( Xuefen Yang ),( Jian Zhang ),( Zongyong Jiang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.6

Probiotics are considered as the best effective alternatives to antibiotics. The aim of this study was to characterize the probiotic potential of lactobacilli for use in swine farming by using in vitro evaluation methods. A total of 106 lactic acid bacterial isolates, originating from porcine feces, were first screened for the capacity to survive stresses considered important for putative probiotic strains. Sixteen isolates showed notable acid and bile resistance, antibacterial activity, and adherence to intestinal porcine epithelial cells (IPEC-1). One isolate, LR1, identified as Lactobacillus reuteri, was selected for extensive study of its probiotic and functional properties in IPEC-1 cell models. L. reuteri LR1 exhibited good adhesion to IPEC-1 cells and could inhibit the adhesion of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells. L. reuteri LR1 could also modulate transcript and protein expression of cytokines involved in inflammation in IPEC-1 cells; the Lactobacillus strain inhibited the ETEC-induced expression of proinflammatory transcripts (IL-6 and TNF-α) and protein (IL-6), and increased the level of anti-inflammatory cytokine (IL-10). Measurement of the permeation of FD-4 showed that L. reuteri LR1 could maintain barrier integrity in monolayer IPEC-1 cells exposed to ETEC. Immunolocalization experiments showed L. reuteri LR1 could also prevent ETEC-induced tight junction ZO-1 disruption. Together, these results indicate that L. reuteri LR1 exhibits desirable probiotic properties and could be a potential probiotic for use in swine production.

Analysis and Optimization of Energy Consumption for Multi-part Printing Using Selective Laser Melting and Considering the Support Structure

Zhilin Ma,Mengdi Gao,Kai Guo,Qingyang Wang,Lei Li,Conghu Liu,Guang Zhu,Zhifeng Liu 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.10 No.3

Selective laser melting (SLM) can form complex and precise metal parts simultaneously and is widely used in medical and aerospace fields. The support structure plays an important role in SLM process, including supporting the overhanging structure, dissipating heat, and minimizing geometric deformation caused by internal stress. However, a non-optimal support structure causes increased energy and material consumption during processing and must be removed afterward to allow for utilization of the parts. Existing support structure design methods only consider reducing the support of a single part, and research on the support and energy consumption of simultaneous multi-part printing is lacking. Therefore, to reduce the energy and material consumption of simultaneous multi-part printing by SLM and improve processing efficiency, an energy consumption analysis and optimization method is proposed in this study from the perspective of the support structure. Based on previous studies on energy consumption distribution of the additive manufacturing process, a multi-component SLM energy consumption and material consumption model was established. Furthermore, a shared-support optimization strategy for simultaneous multi-part processing is proposed. For optimization, the method selects the appropriate printing direction of one part, and then combines multiple parts to form a shared support structure to minimize energy consumption. Finally, under the constraint of minimizing the mass, an optimization strategy of the SLM multi-part shared support combination is established, and the purpose of reducing the energy consumption and material consumption of the SLM is achieved under the premise of ensuring the geometric accuracy of the parts. The method was applied to the manufacturing process of a group of parts with a beam structure. Compared with the printing method using independent support, the shared support structure method reduced energy consumption more than 5.5%, material consumption for the support structure more than 17.2%, and printing time to a certain extent. This method effectively improves SLM production efficiency and sustainability and provides strategic support for additive manufacturing designers and producers.

Short-Term Photovoltaic Power Generation Forecasting Based on Environmental Factors and GA-SVM

Jidong Wang,Ran Ran,Zhilin Song,Jiawen Sun 대한전기학회 2017 Journal of Electrical Engineering & Technology Vol.12 No.1

Considering the volatility, intermittent and random of photovoltaic (PV) generation systems, accurate forecasting of PV power output is important for the grid scheduling and energy management. In order to improve the accuracy of short-term power forecasting of PV systems, this paper proposes a prediction model based on environmental factors and support vector machine optimized by genetic algorithm (GA-SVM). In order to improve the prediction accuracy of this model, weather conditions are divided into three types, and the gray correlation coefficient algorithm is used to find out a similar day of the predicted day. To avoid parameters optimization into local optima, this paper uses genetic algorithm to optimize SVM parameters. Example verification shows that the prediction accuracy in three types of weather will remain at between 10% -15% and the short-term PV power forecasting model proposed is effective and promising.

The characteristics of CO2 front dynamic migration in low permeability sandstone oil reservoirs under different miscibility degrees

Jin Yong,Wang Zhilin,Zhang Zhenxin,Lin Bo,Ge Zhengjun,You Qidong,Chen Hao,Liu Xiliang,Xu Chenghao,Gao Shuaiqiang,Wu Yi,Abro Zafar 한국자원공학회 2023 Geosystem engineering Vol.26 No.4

CO2 flooding is a significant technology for oil recovery, to reveal the microcosmic mechanism of CO2 flooding, CO2 slim tube experiments, long core CO2 flooding experiments, gas chromatography analysis experiments, and numerical simulations were carried out. The results show that the miscibility increase can improve the effect of dissolution and diffusion in the CO2-crude oil system, easing the CO2 finger entry phenomena, and delaying gas breakthrough time. Besides, the CO2 -crude oil interaction is enhanced by increasing the pressure difference between the injection well and the production well, which improves the oil recovery of CO2 flooding. Besides, CO2 carries the light and medium components (C4 ~ 25) forward to cross the first miscible zone, which makes the CO2-crude oil system form double miscible zones. This study reveals the rule of CO2 front and light components front under different miscibility degrees of CO2 flooding from the microscopic perspective and deepens the theory of CO2 flooding in low-permeability sandstone reservoirs.

Gut Microbiota Alteration Influences Colorectal Cancer Metastasis to the Liver by Remodeling the Liver Immune Microenvironment

Yuan Na,Li Xiaoyan,Wang Meng,Zhang Zhilin,Qiao Lu,Gao Yamei,Xu Xinjian,Zhi Jie,Li Yang,Li Zhongxin,Jia Yitao 거트앤리버 소화기연관학회협의회 2022 Gut and Liver Vol.16 No.4

Background/Aims:This study aimed to explore the effect of gut microbiota-regulated Kupffer cells (KCs) on colorectal cancer (CRC) liver metastasis. Methods: A series of in vivo and in vitro researches were showed to demonstrate the gut microbiota and its possible mechanism in CRC liver metastasis. Results: Fewer liver metastases were identified in the ampicillin-streptomycin-colistin and colistin groups. Increased proportions of Parabacteroides goldsteinii, Bacteroides vulgatus, Bacteroides thetaiotaomicron, and Bacteroides uniformis were observed in the colistin group. The significant expansion of KCs was identified in the ampicillin-streptomycin-colistin and colistin groups. B. vulgatus levels were positively correlated with KC levels. More liver metastases were observed in the vancomycin group. An increased abundance of Parabacteroides distasonis and Proteus mirabilis and an obvious reduction of KCs were noted in the vancomycin group. P. mirabilis levels were negatively related to KC levels. The number of liver metastatic nodules was increased in the P. mirabilis group and decreased in the B. vulgatus group. The number of KCs decreased in the P. mirabilis group and increased in the B. vulgatus group. In vitro, as P. mirabilis or B. vulgatus doses increased, there was an opposite effect on KC proliferation in dose- and time-dependent manners. P. mirabilis induced CT26 cell migration by controlling KC proliferation, whereas B. vulgatus prevented this migration. Conclusions: An increased abundance of P. mirabilis and decreased amount of B. vulgatus play key roles in CRC liver metastasis, which might be related to KC reductions in the liver.

Optimization analysis of factors affecting hydrocarbon gas drive based on orthogonal experimental design

Wei Huang,Shenglai Yang,Zhilin Wang,Xiaowei Lv,Hao Lei,Li Chen 한국자원공학회 2014 Geosystem engineering Vol.17 No.6

To improve the ultimate recovery of reservoirs and to increase the economic benefit of oilfields, we feel obliged to study on various factors affecting the development effect of hydrocarbon gas injection and its influence in terms of ultimate recovery. Thus, we can formulate a reasonable and feasible scheme on effective implementation of hydrocarbon gas drive. Take Q28 fault-block as an example; five main factors affecting the development effect of hydrocarbon gas injection have been screened out, four different levels of each influence factor has been set up; the orthogonal experiment method has been adopted to design 16 different combination schemes of 5 factors. Numerical reservoir simulation predicted the final recovery under the different combination schemes. Following are the range analysis and variance analysis of entire results so that the major and minor factors affecting the final recovery and the best range of each factor can be confirmed. The order sequence from major to minor factors: gas injection slug size, gas injection time, gas–water ratio, pressure level, and injection rate. This method is significant on its guidance for hydrocarbon gas injection in this area.

A new integrative evaluation method for candidate reservoirs of hydrocarbon gas drive

Wei Hu,Zhe Wang,Jingchen Ding,Zhilin Wang,Quanzheng Ma,Yu Gao 한국자원공학회 2015 Geosystem engineering Vol.18 No.1

Before implementing hydrocarbon gas drive, an applicable evaluation of reservoirs for hydrocarbon gas drive can improve the success rate and economic benefit. On the basis of a large amount of literature research, 11 factors affecting the hydrocarbon gas injection development have been screened out. These 11 factors constitute the suitability evaluation indexes of candidate reservoirs for hydrocarbon gas drive. Meantime, the classification of 11 evaluation indexes has also been set up, and fuzzy statistical method, analytic hierarchy process, and expert assessment method have been adopted to obtain the weight value of each evaluation index. Three candidate reservoirs have been taken for example; using multi-level fuzzy comprehensive evaluation to optimize the most suitable candidate reservoir, the potential size of three candidate reservoirs for hydrocarbon gas drive is provided. By comparing the evaluation results with the numerical reservoir simulation results, the evaluation method not only gives a correct evaluation for candidate reservoirs but also has a high practicability and reliability.

Application of the French Codes to the Pressurized Thermal Shocks Assessment

Mingya Chen,Guian Qian,Jinhua Shi,Rongshan Wang,Weiwei Yu,Feng Lu,Guodong Zhang,Fei Xue,Zhilin Chen 한국원자력학회 2016 Nuclear Engineering and Technology Vol.48 No.6

The integrity of a reactor pressure vessel (RPV) related to pressurized thermal shocks (PTSs)has been extensively studied. This paper introduces an integrity assessment of an RPVsubjected to a PTS transient based on the French codes. In the USA, the “screening criterion”for maximum allowable embrittlement of RPV material is developed based on theprobabilistic fracture mechanics. However, in the French RCC-M and RSE-M codes, whichare developed based on the deterministic fracture mechanics, there is no “screening criterion”. In this paper, the methodology in the RCC-M and RSE-M codes, which are used forPTS analysis, are firstly discussed. The bases of the French codes are compared with ASMEand FAVOR codes. A case study is also presented. The results show that the method in theRCC-M code that accounts for the influence of cladding on the stress intensity factor (SIF)may be nonconservative. The SIF almost doubles if the weld residual stress is considered. The approaches included in the codes differ in many aspects, which may result in significantdifferences in the assessment results. Therefore, homogenization of the codes inthe long time operation of nuclear power plants is needed.

Assessing Resilience of Inter-Domain Routing System under Regional Failures

( Yujing Liu ),( Wei Peng ),( Jinshu Su ),( Zhilin Wang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.4

Inter-domain routing is the most critical function of the Internet. The routing system is a logical network relying on the physical infrastructure with geographical characteristics. Nature disasters or disruptive accidents such as earthquakes, cable cuts and power outages could cause regional failures which fail down geographically co-located network nodes and links, therefore, affect the resilience of inter-domain routing system. This paper presents a model for regional failures in inter-domain routing system called REFER for the first time. Based on REFER, the resilience of the inter-domain routing system could be evaluated on a finer level of the Internet, considering different routing policies of intra-domain and inter-domain routing systems. Under this model, we perform simulations on an empirical topology of the Internet with geographical characteristics to simulate a regional failure locating at a city with important IXP (Internet eXchange Point). Results indicate that the Internet is robust under a city-level regional failure. The reachability is almost the same after the failure, and the reroutings occur at the edge of the Internet, hardly affecting the core of inter-domain routing system.

Prediction Model of Austenite Growth and the Role of MnS Inclusions in Non- Quenched and Tempered Steel

Bo Jiang,Meng Wu,He Sun,Zhilin Wang,Zhigang Zhao,Yazheng Liu 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.1

The austenite growth behavior of non-quenched and tempered steels (casted by continuous casting and moldingcasting processes) was studied. The austenite grain size of steel B casted by continuous casting process is smallerthan that of steel A casted by molding casting process at the same heating parameters. The abnormal austenitegrowth temperature of the steels A and B are 950 °C and 1000 °C, respectively. Based on the results, the models forthe austenite grain growth below and above the abnormal austenite growth temperature of the investigated steelswere established. The dispersedly distributed fine particles MnS in steel B is the key factor refining the austenitegrain by pinning the migration of austenite grain boundary. The elongated inclusions MnS are ineffective in preventingthe austenite grain growth at high heating temperature. For the non-quenched and tempered steel, the continuouscasting process should be adopted and the inclusion MnS should be elliptical, smaller in size anddistributed uniformly in order to refine the final microstructure and also improve the mechanical properties.