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Tyagi Amrish Kumar,Kumar Sachin,Choudhury Prasanta Kumar,Tyagi Bhawna,Tyagi Nitin 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.8
Objective: The present investigation was aimed to explore the potential of lactobacilli for conjugated linoleic acid (CLA) production, isolated from rumen fluid samples of lactating goats. Methods: A total of 64 isolates of lactobacilli were obtained using deMan-Rogosa-Sharpe (MRS) agar from rumen fluid of goats and further subjected to morphological and biochemical characterizations. Isolates found as gram-positive, catalase negative rods were presumptively identified as Lactobacillus species and further confirmed by genus specific polymerase chain reaction (PCR). The phylogenetic tree was constructed from the nucleotide sequences using MEGA6. Results: Out of the 64 isolates, 23 isolates were observed positive for CLA production by linoleate isomerase gene-based amplification and quantitatively by UV-spectrophotometric assay for the conversion of linoleic acid to CLA as well as gas chromatography-based assay. In all Lactobacillus species cis9, trans11 isomer was observed as the most predominant CLA isomer. These positive isolates were identified by 16S rRNA gene-based PCR sequencing and identified to be different species of L. ingluviei (2), L.salivarius (2), L. curvatus (15), and L. sakei (4). Conclusion: The findings of the present study concluded that lactic acid bacteria isolated from ruminal fluid samples of goat have the potential to produce bioactive CLA and may be applied as a direct fed microbial to enhance the nutraceutical value of animal food products.
Molecular Markers and Their Usefulness in Rice Breeding
( Neeraj Kumar Tyagi ),( Bandarupalli Ramesh ),( Kuldeep Tyagi ) 전북대학교 농업과학기술연구소 2009 농업생명과학연구 Vol.40 No.2
Molecular markers are extensively used for improving and sustaining the rice productivity. A variety of molecular genetic markers, including restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNAs (RAPDs), amplified fragment length polymorphisms (AFLPs), microsatellites or simple sequence repeats (SSRs), expressed sequence tags (EST) and single nucleotide polymorphism (SNP) have been developed providing new tools for rice breeding. The major advantages of the molecular markers over the other classes of markers are their number is potentially unlimited, spanning across the genome, their expression is unaffected by the environment and their assessment is independent of the stage of plant development. Molecular markers are landmarks in the chromosome maps that can be used to monitor the transfer of specific chromosome segments known to carry useful agronomic traits. Breeders use these molecular markers to increase the precision of selection for the best trait combinations. Molecular markers have large number of applications ranging from diversity analysis to the improvement of rice varieties by marker assisted selection. This review describes the usefulness of some important DNA markers in rice improvement.
Tyagi, Kuldeep,Lee, Hyo Jeong,Lee, Chong Ae,Steffenson, Brian J.,Kim, Young Jin,Yun, Song Joong Cambridge University Press 2014 Plant genetic resources Vol.12 No.1
<P>Improved root architecture of cultivated barley can improve crop performance in drought-prone areas. In this study, seedlings of 315 wild barley (<I>Hordeum vulgare</I> subsp. <I>spontaneum</I>) accessions from the Wild Barley Diversity Collection (WBDC) were grown under hydroponic conditions for 8 d after germination and then root characteristics were analysed. Significant differences were observed among the accessions with regard to seminal root number (SRN), root length (RL), specific root length (SRL), root fresh weight and root dry weight (RDW). Principal component analysis explained about 81% of the total variation for ten traits. Principal component (PC) 1, PC2 and PC3 explained about 38, 30 and 13% of the total variation among the accessions. The two most prominent contributors in each PC were RL and SRL, RDW and SRN, and the longitude and latitude of the collection sites, respectively. Accessions WBDC266, WBDC302, WBDC286 and WBDC011 had the longest RL and the highest RDW, specific dry root weight and SRL, respectively. These accessions may be useful genetic resources for the improvement of these root traits in cultivated barley.</P>
The Development of Simple Methods for the Maintenance and Quantification of Polymyxa graminis
Tyagi, S.,Sultana, R.,Ju, H. J.,Lee, W. H.,Kim, K.,Lee, B.,Lee, K. J. Springer Science + Business Media 2016 Indian journal of microbiology Vol.56 No.4
<P>Polymyxa graminis, a root endoparasite of several cereal species, is considered to be non-pathogenic but serves as a vector of various plant viruses belonging to the genera Bymovirus, Furovirus, and Pecluvirus. Specifically, it reduces barley productivity by transmitting the Barley Yellow Mosaic Virus (BaYMV). To date, due to its obligate biotrophic property, no artificial culturing of P. graminis was reported and its quantification was also technically challenging. Here, we developed a novel and simple method to infect P. graminis within sterile barley roots in contamination free by preparing nearly pure zoospore inoculum. Such artificial maintenance of P. graminis was verified based on the presence of various developmental stages in infected barley roots under microscope. In addition, the population of resting spores in host tissue was determined by establishing standard curve between manually counted number of spores and C-t values of 18S rDNA amplification using quantitative real-time PCR. Furthermore, it was validated that standard curve generated was also applicable to estimate the abundance of P. graminis in soil environments. In conclusion, the present study would help to generate a system to investigate the etiological causes as well as management of plant diseases caused by P. graminis and BaYMV in tissue and soil.</P>
Clinical and Social Outcomes of Cochlear Implantation in Older Prelinguals
Tyagi Pragya,Chauhan Divya,Singh Anup,Bhutada Mayank,Sikka Kapil,Chaudhary Tanvi,Sharma Sonam,Agarwal Shivani,Verma Hitesh,Sagar Prem,Kumar Rakesh,Thakar Alok 대한청각학회 2023 Journal of Audiology & Otology Vol.27 No.2
Background and Objectives: Cochlear implantation in late implanted prelinguals necessitates a complex decision-making process for clinicians and patients due to the uncertainty of achieving adequate benefit in auditory and speech perception. This study longitudinally evaluated clinical and social outcomes of prelingually deaf children with implantation in their late childhood.Subjects and Methods: A total of 113 (49 females and 64 males) participants, with an age range of 5-15 years, were assessed for the pre-implant parameters such as hearing loss etiology, aided responses, anatomical aspects, and psychological evaluation. The Category of Auditory Performance, Speech Awareness Threshold, Speech Reception Threshold, and Speech Discrimination Score were administered to assess the patient’s auditory skills. Further, the Speech Intelligibility Rating scale was administered to evaluate the patient’s speech intelligibility at 3, 6, 9, 12, 18, and 24 months post-surgery. Subjectively perceived benefits were evaluated using the satisfaction rating scale and a questionnaire.Results: The statistical results showed a significant impact of cochlear implantation in all domains. Positive impact and improvement post-implantation were noted in all the spheres, including auditory, linguistic, social, and educational.Conclusions: The study highlighted that the outcomes of a cochlear implant at a later age might not parallel with the implantation at a younger age. However, this still provides measurable benefits even after a longer period of auditory deprivation.
Noise Analysis of Sub Quarter Micrometer AlGaN/GaN Microwave Power HEMT
Tyagi, Rajesh K.,Ahlawat, Anil,Pandey, Manoj,Pandey, Sujata The Institute of Electronics and Information Engin 2009 Journal of semiconductor technology and science Vol.9 No.3
An analytical 2-dimensional model to explain the small signal and noise properties of an AlGaN/GaN modulation doped field effect transistor has been developed. The model is based on the solution of two-dimensional Poisson's equation. The developed model explains the influence of Noise in ohmic region (Johnson noise or Thermal noise) as well as in saturated region (spontaneous generation of dipole layers in the saturated region). Small signal parameters are obtained and are used to calculate the different noise parameters. All the results have been compared with the experimental data and show an excellent agreement and the validity of our model.