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Sanghak Yeo,Taeheon Kwon,Changrok Choi,박헌용,현준원,정동근 한국물리학회 2006 Current Applied Physics Vol.6 No.2
For protein chip construction, the chemical properties for the surface of glass slides have to be elaborately modified for protein immobilization. To immobilize proteins, plasma polymerized hexamethyldisiloxane (PPHMDSO) thin film was deposited onto the surface of glass slide. Previously, it was reported that proteins were immobilized onto PPHMDSO thin film [H. Miyachi, A. Hiratsuka, K. Ikebukuro, K. Yano, H. Muguruma, I. Karube, Biotechnol. Bioeng. 69 (3) (2000) 323–329]. Here, we modified PPHMDSO-coated glass slide by an additional oxygen plasma treatment with placing the patterned mask on the HMDSO-coated glass slide. Hydrophilic surfaces were detected by measuring the contact angle of water. Of great interest, proteins were strictly immobilized on the patterned O2 plasma treated areas, providing more efficient ways for fabricating the protein chips. For protein chip construction, the chemical properties for the surface of glass slides have to be elaborately modified for protein immobilization. To immobilize proteins, plasma polymerized hexamethyldisiloxane (PPHMDSO) thin film was deposited onto the surface of glass slide. Previously, it was reported that proteins were immobilized onto PPHMDSO thin film [H. Miyachi, A. Hiratsuka, K. Ikebukuro, K. Yano, H. Muguruma, I. Karube, Biotechnol. Bioeng. 69 (3) (2000) 323–329]. Here, we modified PPHMDSO-coated glass slide by an additional oxygen plasma treatment with placing the patterned mask on the HMDSO-coated glass slide. Hydrophilic surfaces were detected by measuring the contact angle of water. Of great interest, proteins were strictly immobilized on the patterned O2 plasma treated areas, providing more efficient ways for fabricating the protein chips.
Yeo, Sanghak,Kwon, Taeheon,Choi, Changrok,Park, Heonyong,Hyun, June Won,Jung, Donggeun Elsevier 2006 CURRENT APPLIED PHYSICS Vol.6 No.2
<P><B>Abstract</B></P><P>For protein chip construction, the chemical properties for the surface of glass slides have to be elaborately modified for protein immobilization. To immobilize proteins, plasma polymerized hexamethyldisiloxane (PPHMDSO) thin film was deposited onto the surface of glass slide. Previously, it was reported that proteins were immobilized onto PPHMDSO thin film [H. Miyachi, A. Hiratsuka, K. Ikebukuro, K. Yano, H. Muguruma, I. Karube, Biotechnol. Bioeng. 69 (3) (2000) 323–329]. Here, we modified PPHMDSO-coated glass slide by an additional oxygen plasma treatment with placing the patterned mask on the HMDSO-coated glass slide. Hydrophilic surfaces were detected by measuring the contact angle of water. Of great interest, proteins were strictly immobilized on the patterned O<SUB>2</SUB> plasma treated areas, providing more efficient ways for fabricating the protein chips.</P>