http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Sanghak Yeo (검색결과 6 건)
- 무료
- 기관 내 무료
- 유료
-
Sanghak Yeo,Taeheon Kwon,Changrok Choi,박헌용,현준원,정동근 한국물리학회 2006 Current Applied Physics Vol.6 No.2
For protein chip construction, the chemical properties for the surface of glass slides have to be elaborately modified for protein immobilization. To immobilize proteins, plasma polymerized hexamethyldisiloxane (PPHMDSO) thin film was deposited onto the surface of glass slide. Previously, it was reported that proteins were immobilized onto PPHMDSO thin film [H. Miyachi, A. Hiratsuka, K. Ikebukuro, K. Yano, H. Muguruma, I. Karube, Biotechnol. Bioeng. 69 (3) (2000) 323–329]. Here, we modified PPHMDSO-coated glass slide by an additional oxygen plasma treatment with placing the patterned mask on the HMDSO-coated glass slide. Hydrophilic surfaces were detected by measuring the contact angle of water. Of great interest, proteins were strictly immobilized on the patterned O2 plasma treated areas, providing more efficient ways for fabricating the protein chips. For protein chip construction, the chemical properties for the surface of glass slides have to be elaborately modified for protein immobilization. To immobilize proteins, plasma polymerized hexamethyldisiloxane (PPHMDSO) thin film was deposited onto the surface of glass slide. Previously, it was reported that proteins were immobilized onto PPHMDSO thin film [H. Miyachi, A. Hiratsuka, K. Ikebukuro, K. Yano, H. Muguruma, I. Karube, Biotechnol. Bioeng. 69 (3) (2000) 323–329]. Here, we modified PPHMDSO-coated glass slide by an additional oxygen plasma treatment with placing the patterned mask on the HMDSO-coated glass slide. Hydrophilic surfaces were detected by measuring the contact angle of water. Of great interest, proteins were strictly immobilized on the patterned O2 plasma treated areas, providing more efficient ways for fabricating the protein chips.
-
Investigation of Protein Adsorption Using Plasma Treatment for Protein Chips
Sanghak Yeo,정동근,Changrok Choi,박헌용 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.48 No.6
For reliable protein chip performance, a sufficient level of proteins must be immobilized in a specific area on the surface of the chip. In this research, a plasma-polymerized hexamethyldisiloxane (PPHMDSO) thin film was deposited on the surface of a glass slide. The PPHMDSO-coated glass slide was modified by an additional oxygen plasma treatment with the patterned mask placed on the PPHMDSO-coated glass slide. The deposited PPHMDSO was hydrophobic and did not show adsorbed proteins, and the O2-plasma-treated areas of PPHMDSO were hydrophilic. The proteins were strictly adsorbed and immobilized on the patterned O2-plasma-treated areas of PPHMDSO, providing a more efficient way of fabricating high-density protein chips. The change in the O2-plasma-treated PPHMDSO surface was analyzed, and a possible reason for selective protein adsorption is suggested. For reliable protein chip performance, a sufficient level of proteins must be immobilized in a specific area on the surface of the chip. In this research, a plasma-polymerized hexamethyldisiloxane (PPHMDSO) thin film was deposited on the surface of a glass slide. The PPHMDSO-coated glass slide was modified by an additional oxygen plasma treatment with the patterned mask placed on the PPHMDSO-coated glass slide. The deposited PPHMDSO was hydrophobic and did not show adsorbed proteins, and the O2-plasma-treated areas of PPHMDSO were hydrophilic. The proteins were strictly adsorbed and immobilized on the patterned O2-plasma-treated areas of PPHMDSO, providing a more efficient way of fabricating high-density protein chips. The change in the O2-plasma-treated PPHMDSO surface was analyzed, and a possible reason for selective protein adsorption is suggested.
-
Sanghak Yeo,박헌용,Jaeyoung Yang,부진효,정동근,Changrok Choi 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.51 No.3
Nonspecific adsorption to the surface of slides decreases the sensitivity for chip-based biological assays. To solve this problem, we constructed novel patterned slides of plasma polymerized ethylenediamine (PPEDA) with protein-binding amine functional groups and a hydrophilic surface and of plasma polymerized cyclohexane (PPCHex) with a hydrophobic surface and a reduced nonspecific protein adsorption. PPEDA and PPCHex were deposited by using inductively coupled plasma chemical vapor deposition (ICP-CVD) with ethylenediamine (EDA) and cyclohexane (CHex) as precursors. PPEDA was deposited in a patterned manner on a PPCHex slide by using plasma polymerization with a patterned mask. Comparing the sample of the PPEDA/PPCHex pattern with the sample of PPEDA only, i.e., the sample with only PPEDA spots formed on bare glass slides, the average signal to noise ratio, defined as the ratio of the fluorescence intensity of the PPEDA-deposited circular spots to the fluorescence intensity of the surrounding areas, was higher for the sample with the PPEDA/PPCHex pattern, indicating that the nonspecific adsorption was reduced at the surface of PPCHex. It is thought that on the surface of PPCHex films, the reduction in the protein adsorption was more influenced by the chemical groups of the films, such as -OH groups, rather than by the physical properties of the surfaces, such as the roughness.s Nonspecific adsorption to the surface of slides decreases the sensitivity for chip-based biological assays. To solve this problem, we constructed novel patterned slides of plasma polymerized ethylenediamine (PPEDA) with protein-binding amine functional groups and a hydrophilic surface and of plasma polymerized cyclohexane (PPCHex) with a hydrophobic surface and a reduced nonspecific protein adsorption. PPEDA and PPCHex were deposited by using inductively coupled plasma chemical vapor deposition (ICP-CVD) with ethylenediamine (EDA) and cyclohexane (CHex) as precursors. PPEDA was deposited in a patterned manner on a PPCHex slide by using plasma polymerization with a patterned mask. Comparing the sample of the PPEDA/PPCHex pattern with the sample of PPEDA only, i.e., the sample with only PPEDA spots formed on bare glass slides, the average signal to noise ratio, defined as the ratio of the fluorescence intensity of the PPEDA-deposited circular spots to the fluorescence intensity of the surrounding areas, was higher for the sample with the PPEDA/PPCHex pattern, indicating that the nonspecific adsorption was reduced at the surface of PPCHex. It is thought that on the surface of PPCHex films, the reduction in the protein adsorption was more influenced by the chemical groups of the films, such as -OH groups, rather than by the physical properties of the surfaces, such as the roughness.s
-
Yeo, Sanghak,Kwon, Taeheon,Choi, Changrok,Park, Heonyong,Hyun, June Won,Jung, Donggeun Elsevier 2006 CURRENT APPLIED PHYSICS Vol.6 No.2
<P><B>Abstract</B></P><P>For protein chip construction, the chemical properties for the surface of glass slides have to be elaborately modified for protein immobilization. To immobilize proteins, plasma polymerized hexamethyldisiloxane (PPHMDSO) thin film was deposited onto the surface of glass slide. Previously, it was reported that proteins were immobilized onto PPHMDSO thin film [H. Miyachi, A. Hiratsuka, K. Ikebukuro, K. Yano, H. Muguruma, I. Karube, Biotechnol. Bioeng. 69 (3) (2000) 323–329]. Here, we modified PPHMDSO-coated glass slide by an additional oxygen plasma treatment with placing the patterned mask on the HMDSO-coated glass slide. Hydrophilic surfaces were detected by measuring the contact angle of water. Of great interest, proteins were strictly immobilized on the patterned O<SUB>2</SUB> plasma treated areas, providing more efficient ways for fabricating the protein chips.</P>
-
-
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
