Surface analysis of protein‐resistant, plasma‐polymerized ethylene glycol thin films

Choi, Changrok,Jung, Donggeun,Moon, Dae Won,Lee, Tae Geol John Wiley Sons, Ltd. 2011 Surface and interface analysis Vol.43 No.1

<P><B>Abstract</B></P><P>Poly(ethylene glycol) (PEG) has been widely used in biomedical applications because of its protein‐resistant and nontoxic properties. In this study, a plasma‐polymerized ethylene glycol (PPEG) thin film was deposited on a substrate using a capacitively coupled plasma chemical vapour deposition (CCP‐CVD) method with various plasma powers and ethylene glycol as a precursor. The surfaces of the PPEG thin films were characterized using time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) along with a principal component analysis (PCA), and XPS. We confirmed that the PPEG thin film deposited at low plasma power closely resembles PEG polymers in terms of surface chemical composition and protein‐resistant property. To make patterned surfaces with protein‐resistant property for proteins, a PPEG thin film was first uniformly deposited on a glass slide and patterns were produced on the PPEG coated surface by depositing a plasma‐polymerized amine film through a metal shadow mask. When we spotted proteins on the patterned surface, they were immobilized only onto the plasma‐polymerized amine areas but not onto the PPEG areas. These results show that PPEG surfaces would be useful for the construction of various protein arrays. Copyright © 2010 John Wiley & Sons, Ltd.</P>

Culturing of Rat Intestinal Epithelial Cells-18 on Plasma Polymerized Ethylenediamine Films Deposited by Plasma Enhanced Chemical Vapor Deposition

Changrok Choi,Kyung Seop Kim,Hong Ja Kim,Heonyong Park,정동근,Jin-Hyo Boo 대한화학회 2009 Bulletin of the Korean Chemical Society Vol.30 No.6

Many researchers studied cell culturing on surfaces with chemical functional groups. Previously, we reported surface properties of plasma polymerized ethylenediamine (PPEDA) films deposited by plasma enhanced chemical vapor deposition with various plasma conditions. Surface properties of PPEDA films can be controlled by plasma power during deposition. In this work, to analyze correlation of cell adherence/proliferation with surface property, we cultured rat intestinal epithelial cells-18 on the PPEDA films deposited with various plasma powers. It was shown that as plasma power was decreased, density of cells cultured on the PPEDA film surface was increased. Our findings indicate that plasma power changed the amine density of the PPEDA film surface, resulting in density change of cells cultured on the PPEDA film surface.

Fabrication and Characterization of Plasma-Polymerized Poly(ethylene glycol) Film with Superior Biocompatibility

Choi, Changrok,Hwang, Inseong,Cho, Young-Lai,Han, Sang Y.,Jo, Dong H.,Jung, Donggeun,Moon, Dae W.,Kim, Eun J.,Jeon, Chang S.,Kim, Jeong H.,Chung, Taek D.,Lee, Tae G. American Chemical Society 2013 ACS APPLIED MATERIALS & INTERFACES Vol.5 No.3

<P>A newly fabricated plasma-polymerized poly(ethylene glycol) (PP-PEG) film shows extremely low toxicity, low fouling, good durability, and chemical similarity to typical PEG polymers, enabling live cell patterning as well as various bioapplications using bioincompatible materials. The PP-PEG film can be overlaid on any materials via the capacitively coupled plasma chemical vapor deposition (CCP-CVD) method using nontoxic PEG200 as a precursor. The biocompatibility of the PP-PEG-coated surface is confirmed by whole blood flow experiments where no thrombi and less serum protein adsorption are observed when compared with bare glass, polyethylene (PE), and polyethylene terephthalate (PET) surfaces. Furthermore, unlike bare PE films, less fibrosis and inflammation are observed when the PP-PEG-coated PE film is implanted into subcutaneous pockets of mice groin areas. The cell-repellent property of PP-PEG is also verified via patterning of mammalian cells, such as fibroblasts and hippocampal neurons. These results show that our PP-PEG film, generated by the CCP-CVD method, is a biocompatible material that can be considered for broad applications in biomedical and functional materials fields.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2013/aamick.2013.5.issue-3/am302208f/production/images/medium/am-2012-02208f_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am302208f'>ACS Electronic Supporting Info</A></P>

Attachment and Growth of Bovine Aortic Endothelial Cells on Amine Surfaces Formed by Using Plasma-Enhanced Chemical-Vapor Deposition

정동근,Changrok Choi,Sung Il Kim,,인경민,박헌용 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.3

Cells attached on surfaces can be used for many biological applications, such as cell chips. For some purposes, surfaces with excellent cell attachment and subsequent growth are needed. In this work, plasma-polymerized ethylenediamine (PPEDA) thin films were deposited on glass slides by using a plasma-enhanced chemical-vapor deposition system with an ethylenediamine precursor. In previous reports, PPEDA thin films were shown to have a high density of amine functional groups on their surfaces. Bovine aortic endothelial cells (BAECs) tested in this study were grown on a PPEDA film in Dulbecco's modified Eagle's medium containing 20 % Fetal Bovine Serum at 37 ℃ and 5 % CO2. The BAECs were revealed to have adhered to the surface of the PPEDA film and to have grown as on commercial culture dishes. By estimating the cell growth rates, we found the PPEDA film to be better than commercial culture dishes. The PPEDA film can be applied for a variety of biological assay techniques.

아브라함계 종교와 세계인권선언: 인권관념 비교연구

서창록 ( Soh Changrok ),최정음 ( Choi Jeongeum ) 서울대학교 종교문제연구소 2021 종교와 문화 Vol.- No.40

This article compares and analyzes the idea of human rights in Abrahamic religions. For this purpose, it reviews Abramamic religions’ contribution to the adoption of the Universal Declaration of Human Rights (UDHR), their theological interpretations of rights-claiming culture, and human dignity. The article identifies two components that have shaped their varied notion of human rights. The first is that the religions have regarded the universalist claim of human rights as a moral challenge against their religious doctrines, which was established to preserve their Divine revelations. The second is that their historical experiences - religious persecution for Judaism, the Enlightenment for Christianity, and the West European Colonialism for Islam - have respectively shaped their initial hostility towards the idea of human rights. The concept of human rights is commonly considered to be founded on the Judeo-Christian tradition and the Western experience of the Enlightenment. Judaism and Christianity, however, alongside Islam, have in actuality often repudiated the idea of universal human rights following the adoption of the UDHR. This paper demonstrates that this conceptual distance between religion and human rights has ultimately been narrowed due to an increase in shared dialogue. In short, the idea of human rights has earned its universality in Abrahamic religions through a set of debates and compromises.

The patterned hydrophilic surfaces of glass slides to be applicable for the construction of protein chips

Yeo, Sanghak,Kwon, Taeheon,Choi, Changrok,Park, Heonyong,Hyun, June Won,Jung, Donggeun Elsevier 2006 CURRENT APPLIED PHYSICS Vol.6 No.2

<P><B>Abstract</B></P><P>For protein chip construction, the chemical properties for the surface of glass slides have to be elaborately modified for protein immobilization. To immobilize proteins, plasma polymerized hexamethyldisiloxane (PPHMDSO) thin film was deposited onto the surface of glass slide. Previously, it was reported that proteins were immobilized onto PPHMDSO thin film [H. Miyachi, A. Hiratsuka, K. Ikebukuro, K. Yano, H. Muguruma, I. Karube, Biotechnol. Bioeng. 69 (3) (2000) 323–329]. Here, we modified PPHMDSO-coated glass slide by an additional oxygen plasma treatment with placing the patterned mask on the HMDSO-coated glass slide. Hydrophilic surfaces were detected by measuring the contact angle of water. Of great interest, proteins were strictly immobilized on the patterned O<SUB>2</SUB> plasma treated areas, providing more efficient ways for fabricating the protein chips.</P>

The patterned hydrophilic surfaces of glass slides to be applicable for the construction of protein chips

Sanghak Yeo,Taeheon Kwon,Changrok Choi,박헌용,현준원,정동근 한국물리학회 2006 Current Applied Physics Vol.6 No.2

For protein chip construction, the chemical properties for the surface of glass slides have to be elaborately modified for protein immobilization. To immobilize proteins, plasma polymerized hexamethyldisiloxane (PPHMDSO) thin film was deposited onto the surface of glass slide. Previously, it was reported that proteins were immobilized onto PPHMDSO thin film [H. Miyachi, A. Hiratsuka, K. Ikebukuro, K. Yano, H. Muguruma, I. Karube, Biotechnol. Bioeng. 69 (3) (2000) 323–329]. Here, we modified PPHMDSO-coated glass slide by an additional oxygen plasma treatment with placing the patterned mask on the HMDSO-coated glass slide. Hydrophilic surfaces were detected by measuring the contact angle of water. Of great interest, proteins were strictly immobilized on the patterned O2 plasma treated areas, providing more efficient ways for fabricating the protein chips. For protein chip construction, the chemical properties for the surface of glass slides have to be elaborately modified for protein immobilization. To immobilize proteins, plasma polymerized hexamethyldisiloxane (PPHMDSO) thin film was deposited onto the surface of glass slide. Previously, it was reported that proteins were immobilized onto PPHMDSO thin film [H. Miyachi, A. Hiratsuka, K. Ikebukuro, K. Yano, H. Muguruma, I. Karube, Biotechnol. Bioeng. 69 (3) (2000) 323–329]. Here, we modified PPHMDSO-coated glass slide by an additional oxygen plasma treatment with placing the patterned mask on the HMDSO-coated glass slide. Hydrophilic surfaces were detected by measuring the contact angle of water. Of great interest, proteins were strictly immobilized on the patterned O2 plasma treated areas, providing more efficient ways for fabricating the protein chips.

Investigation of Protein Adsorption Using Plasma Treatment for Protein Chips

Sanghak Yeo,정동근,Changrok Choi,박헌용 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.48 No.6

For reliable protein chip performance, a sufficient level of proteins must be immobilized in a specific area on the surface of the chip. In this research, a plasma-polymerized hexamethyldisiloxane (PPHMDSO) thin film was deposited on the surface of a glass slide. The PPHMDSO-coated glass slide was modified by an additional oxygen plasma treatment with the patterned mask placed on the PPHMDSO-coated glass slide. The deposited PPHMDSO was hydrophobic and did not show adsorbed proteins, and the O2-plasma-treated areas of PPHMDSO were hydrophilic. The proteins were strictly adsorbed and immobilized on the patterned O2-plasma-treated areas of PPHMDSO, providing a more efficient way of fabricating high-density protein chips. The change in the O2-plasma-treated PPHMDSO surface was analyzed, and a possible reason for selective protein adsorption is suggested. For reliable protein chip performance, a sufficient level of proteins must be immobilized in a specific area on the surface of the chip. In this research, a plasma-polymerized hexamethyldisiloxane (PPHMDSO) thin film was deposited on the surface of a glass slide. The PPHMDSO-coated glass slide was modified by an additional oxygen plasma treatment with the patterned mask placed on the PPHMDSO-coated glass slide. The deposited PPHMDSO was hydrophobic and did not show adsorbed proteins, and the O2-plasma-treated areas of PPHMDSO were hydrophilic. The proteins were strictly adsorbed and immobilized on the patterned O2-plasma-treated areas of PPHMDSO, providing a more efficient way of fabricating high-density protein chips. The change in the O2-plasma-treated PPHMDSO surface was analyzed, and a possible reason for selective protein adsorption is suggested.

Patterned Amine Surfaces with Reduced Background Nonspecific Protein Adsorption Fabricated by Using Inductively Coupled Plasma Chemical Vapor Deposition

Sanghak Yeo,박헌용,Jaeyoung Yang,부진효,정동근,Changrok Choi 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.51 No.3

Nonspecific adsorption to the surface of slides decreases the sensitivity for chip-based biological assays. To solve this problem, we constructed novel patterned slides of plasma polymerized ethylenediamine (PPEDA) with protein-binding amine functional groups and a hydrophilic surface and of plasma polymerized cyclohexane (PPCHex) with a hydrophobic surface and a reduced nonspecific protein adsorption. PPEDA and PPCHex were deposited by using inductively coupled plasma chemical vapor deposition (ICP-CVD) with ethylenediamine (EDA) and cyclohexane (CHex) as precursors. PPEDA was deposited in a patterned manner on a PPCHex slide by using plasma polymerization with a patterned mask. Comparing the sample of the PPEDA/PPCHex pattern with the sample of PPEDA only, i.e., the sample with only PPEDA spots formed on bare glass slides, the average signal to noise ratio, defined as the ratio of the fluorescence intensity of the PPEDA-deposited circular spots to the fluorescence intensity of the surrounding areas, was higher for the sample with the PPEDA/PPCHex pattern, indicating that the nonspecific adsorption was reduced at the surface of PPCHex. It is thought that on the surface of PPCHex films, the reduction in the protein adsorption was more influenced by the chemical groups of the films, such as -OH groups, rather than by the physical properties of the surfaces, such as the roughness.s Nonspecific adsorption to the surface of slides decreases the sensitivity for chip-based biological assays. To solve this problem, we constructed novel patterned slides of plasma polymerized ethylenediamine (PPEDA) with protein-binding amine functional groups and a hydrophilic surface and of plasma polymerized cyclohexane (PPCHex) with a hydrophobic surface and a reduced nonspecific protein adsorption. PPEDA and PPCHex were deposited by using inductively coupled plasma chemical vapor deposition (ICP-CVD) with ethylenediamine (EDA) and cyclohexane (CHex) as precursors. PPEDA was deposited in a patterned manner on a PPCHex slide by using plasma polymerization with a patterned mask. Comparing the sample of the PPEDA/PPCHex pattern with the sample of PPEDA only, i.e., the sample with only PPEDA spots formed on bare glass slides, the average signal to noise ratio, defined as the ratio of the fluorescence intensity of the PPEDA-deposited circular spots to the fluorescence intensity of the surrounding areas, was higher for the sample with the PPEDA/PPCHex pattern, indicating that the nonspecific adsorption was reduced at the surface of PPCHex. It is thought that on the surface of PPCHex films, the reduction in the protein adsorption was more influenced by the chemical groups of the films, such as -OH groups, rather than by the physical properties of the surfaces, such as the roughness.s

Quantitative Mass Spectrometric Analysis of Mixed Self-Assembled Monolayers for Biochips

Jin Gyeong Son,Hyun Kyong Shon,Daewha Hong,Changrok Choi,Sang Woo Han,Insung S. Choi,Tae Geol Lee 한국진공학회 2013 한국진공학회 학술발표회초록집 Vol.2013 No.2