Calibrations

Tasaki, Hiroyuki TOPOLOGY AND GEOMETRY RESEARCH CENTER 1991 Proceedings of the Topology and Geometry Research Vol.2 No.-

INTEGRAL GEOMETRY IN SYMMETRIC SPACES

TASAKI, HIROYUKI TOPOLOGY AND GEOMETRY RESEARCH CENTER 1995 Proceedings of the Topology and Geometry Research Vol.6 No.-

UBR box N-recognin-4 (UBR4), an N-recognin of the N-end rule pathway, and its role in yolk sac vascular development and autophagy

Tasaki, Takafumi,Kim, Sung Tae,Zakrzewska, Adriana,Lee, Bo Eun,Kang, Min Jueng,Yoo, Young Dong,Cha-Molstad, Hyun Joo,Hwang, Joonsung,Soung, Nak Kyun,Sung, Ki Sa,Kim, Su-Hyeon,Nguyen, Minh Dang,Sun, Mi National Academy of Sciences 2013 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.110 No.10

<P>The N-end rule pathway is a proteolytic system in which destabilizing N-terminal residues of short-lived proteins act as degradation determinants (N-degrons). Substrates carrying N-degrons are recognized by N-recognins that mediate ubiquitylation-dependent selective proteolysis through the proteasome. Our previous studies identified the mammalian N-recognin family consisting of UBR1/E3α, UBR2, UBR4/p600, and UBR5, which recognize destabilizing N-terminal residues through the UBR box. In the current study, we addressed the physiological function of a poorly characterized N-recognin, 570-kDa UBR4, in mammalian development. UBR4-deficient mice die during embryogenesis and exhibit pleiotropic abnormalities, including impaired vascular development in the yolk sac (YS). Vascular development in UBR4-deficient YS normally advances through vasculogenesis but is arrested during angiogenic remodeling of primary capillary plexus associated with accumulation of autophagic vacuoles. In the YS, UBR4 marks endoderm-derived, autophagy-enriched cells that coordinate differentiation of mesoderm-derived vascular cells and supply autophagy-generated amino acids during early embryogenesis. UBR4 of the YS endoderm is associated with a tissue-specific autophagic pathway that mediates bulk lysosomal proteolysis of endocytosed maternal proteins into amino acids. In cultured cells, UBR4 subpopulation is degraded by autophagy through its starvation-induced association with cellular cargoes destined to autophagic double membrane structures. UBR4 loss results in multiple misregulations in autophagic induction and flux, including synthesis and lipidation/activation of the ubiquitin-like protein LC3 and formation of autophagic double membrane structures. Our results suggest that UBR4 plays an important role in mammalian development, such as angiogenesis in the YS, in part through regulation of bulk degradation by lysosomal hydrolases.</P>

The N-End Rule Pathway

Tasaki, Takafumi,Sriram, Shashikanth M.,Park, Kyong Soo,Kwon, Yong Tae Annual Reviews 2012 Annual review of biochemistry Vol.81 No.-

<P>The N-end rule pathway is a proteolytic system in which N-terminal residues of short-lived proteins are recognized by recognition components (N-recognins) as essential components of degrons, called N-degrons. Known N-recognins in eukaryotes mediate protein ubiquitylation and selective proteolysis by the 26S proteasome. Substrates of N-recognins can be generated when normally embedded destabilizing residues are exposed at the N terminus by proteolytic cleavage. N-degrons can also be generated through modifications of posttranslationally exposed pro-N-degrons of otherwise stable proteins; such modifications include oxidation, arginylation, leucylation, phenylalanylation, and acetylation. Although there are variations in components, degrons, and hierarchical structures, the proteolytic systems based on generation and recognition of N-degrons have been observed in all eukaryotes and prokaryotes examined thus far. The N-end rule pathway regulates homeostasis of various physiological processes, in part, through interaction with small molecules. Here, we review the biochemical mechanisms, structures, physiological functions, and small-molecule-mediated regulation of the N-end rule pathway.</P>

2000년대 아세아의 축산업 전망

Tasaki, Iwao 한국축산학회 1986 한국축산학회지 Vol.28 No.5

The world population has grown very rapidly, and food production has to be increased for feeding humans. In this sence, animal production should be emphasized. Therefore, how to increase animal products is a most problem in animal agriculture in the year 2000. Although many difficulties are existent, effective ways to improve the animal production has to be explored. Among them, improvement of bioecological and socio-economic conditions seems to be most important. In general, it is not so difficult to develop productions of mono-gastric animals such as poultry and pigs, because these animals can be produced intensively and private sectors can manage the industry effectively. It does not take longer time to develop this kind of industry, however, developing the industry of large animals such as dairy and beef cattle needs longer time and large investment. Therefore it is rather difficult to develop such the industry by the private sectors, and governmental support should be necessary. For developing animal production in Asia, especially in the tropical Asian countries, properly arranged education and extension services are quite important. Otherwise, the new technology and knowledge that have recently been developed can not be utilized in the practical fields.

Shoulder Injection for Painful Shoulder is Effective and Also Painless than Expected

Atsushi TASAKI,Wataru MORITA,Yoshimitsu HOSHIKAWA 대한견주관절의학회 2009 대한견주관절학회 학술대회논문집 Vol.2009 No.5

Gene cloning of a Pleurotus ostreatus lipoxygenase involved in the biosynthesis of mushroom aroma

Yuji Tasaki,Shungo Toyama,Toshio Joh 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4

Lipoxygenase (LOX) is considered to be a key enzyme in the biosynthetic pathways of the most important mushroom aroma, 1-octen-3-ol. In previous work, we purified and characterized a LOX from Pleurotus ostreatus (probably H1 strain) fruit bodies [1] and also determined its partial amino acid sequence. In this study, to clarify the biosynthetic mechanism of 1-octen-3-ol, we isolated cDNA and genomic DNA corresponding to a LOX (Polox1) gene of P. ostreatus H1, and analyzed the expression of the gene in the fruit bodies. A commercial P. ostreatus H1 strain (Onuki kinjin, Utsunomiya, Japan) was used in this study. To isolate the Polox1 cDNA, RT-PCR was done using degenerate primers designed from the partial amino acid sequence. This approach generated a single DNA band of approximately 1.1 kbp, which was cloned and sequenced. The deduced amino acid sequence showed high similarity to LOXs of some ascomycetes fungi. To obtain the full-length cDNA of Polox1, clones corresponding to the Polox1 gene were isolated by plaque hybridization from a cDNA library of the P. ostreatus H1 fruit body. DNA sequences of all clones were determined. The 5’ end of the Polox1 cDNA was amplified by the 5’ RACE method and cloned. The full-length cDNA of Polox1 is 2,031 bp long and contains 640 amino acid residues. The deduced amino acid sequence contains LOX iron-binding catalytic domain signature sequences. Next, to determine the genomic DNA sequence of the Polox1 gene, inverse PCR and PCR was done with P. ostreatus H1 genomic DNA. After inverse PCR and PCR, 3.3 and 1.9 kbp DNA fragments, respectively, were amplified and sequenced. Sequence comparison between cDNA and genomic DNA showed that Polox1 gene contained one intron. To investigate expression of the Polox1 gene, northern blot analysis and measurement of LOX activity were performed. P. ostreatus fruit bodies were produced in a sawdust medium containing beech sawdust and rice bran and separated into pileus and stipe. Two transcripts were detected by northern blot analysis in both pileus and stipe. The band intensities were relatively higher in the stipe than in the pileus. The level of LOX activity in the stipe was 3.8 times higher than that in the pileus. By Southern blot analysis, several major bands were detected after the digestion of 4 restriction enzymes. These blot analyses suggest that the Polox1 gene is probably a member of a small gene family. [1] T. Kuribayashi et al., J. Agric. Food Chem., 50, 1247 (2002).

日本の江戶時代の地方の醫者について

田崎哲郞 ( Tasaki Tetsuro ) 경북대학교 영남문화연구원 2012 嶺南學 Vol.0 No.21

건강은 인류에 있어서 어떤 시대에도 중요한 관심사이다. 봉건영주(大名, 藩)가 지배했던 일본 에도시대에 일반 사람들은 병, 특히 역병에 영향을 받은 적이 많았다. 그 때문에 병을 취급하는 의사의 역할은 컸다. 번(藩)에는 藩醫가 있으며, 큰 읍에는 町醫가 일찍부터 있었는데, 지방의 농촌에도 의사는 있었던 것일까? 있었다고 한다면 언제부터, 어떤 형태로 있었던 것일까? 많은 제자를 양성했던 醫學塾에 남아있는 문하생들의 장부(門人帳)에서, 내가 거주하고 있는 아이치(愛知)현 동남부 미카와(三河)지방 출신의 문하생을 찾아 직접 걸어가서 문하생의 자손을 방문하여 얻은 지식을 덧붙여서 이 지역에 점차적으로 의사가 늘어나고 있는 양상을 고찰해 보고자 한다. 고도의 지식을 요구하는 의사가 배출되기 위해서는 문자를 읽을 수 있는 사람이 지역에 널리 존재하는 것이 전제가 된다. 그것은 테라꼬야(寺子屋: 서당)나 하이쿠(俳句: 일본 고유의 정형화된 詩)의 보급에 의해서 형성되어 온 것 같다. 그리고 한문을 웬만하게 읽을 수 있는 자가 보다 더 앞선 유학을 배우게 되며 그 중에서 의학을 지망하는 자가 나왔던 것 같다. 처음에는 한방의학이 중심이었지만, 에도(江戶)시대 후기가 되면 서양의 의학도 배우게 된다. 그들은 네덜란드의 의학을 배웠기 때문에 란보이(蘭方醫)라고 불렀다. 란보이의 최대 공적은 우두종두법(牛痘種痘法)을 도입한 것이었다. 이에 따라 아이를 가진 부모들은 안심을 할 수 있었으며, 마을의 인구도 안정을 찾게 되었다고 말할 수 있을 것이다. 우두법의 침투는 아시아의 지역에 따라서 차이가 있는 것 같다. 그 차이로부터 문화의 형성에 차이를 엿볼 수는 없는 것일까? Humanity in every age has been concerned about their health. People in the Japanese Edo Era feudal lords often ruled lands that were affected by the plague. As a result the roles of doctors who treated these diseases were important and essential. There were doctors in feudal domains (a feudal domain doctor, 藩醫) and in towns (a town doctor, 町醫). As an extension, a logical question would be whether or not there were village doctors. If there were, further question regarding when they came into existence, and how they subsisted could also be asked. I attempted to research the aspect of the increasing number of doctors in Mikawa Province, southeast of Aichi Prefecture with reference to the registration book of the medical school which produced many doctors, and the information from the descendents of the medical school students in Mikawa Province. A number of learned people were an essential prerequisite for having a doctor in an area that requires broad knowledge and skill. It seemed possible through the popularization of Terakoya (寺子屋, private village schools) and Haiku (俳句, Japanese traditional poems). It can be estimated that students who had been instructed in Chinese characters could learn the advanced study of Confucianism, specifically medicine. At first, they tended to concentrate on oriental medicine and in the late Edo Era, they began to study western medicine. The doctors were called "Lanboi" (蘭方醫) as they learned based on Dutch medical practices. It could be said that the introduction of vaccinations was one of their most notable feats, which reassured parents at that time and stabilized the population. In Asia the diffusion of vaccinations shows in its regional variation. It might come from differences of cultural patterns of each countries.

The Group of Tangent Vectors to a Lie Group and the Differential of Its Exponential Mapping

Hiroyuki Tasaki KYUNGPOOK UNIVERSITY 1998 Kyungpook mathematical journal Vol.38 No.1

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第四發表 : 世界から見た日本の初等敎員養成

전기덕우 ( Tasaki Noritomo ) 한국초등교육학회 2006 학술대회 Vol.2006 No.1