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( Sung Won Lee ),( Dong Wook Choi ),( Sung Chul Park ),( Hee Jung Kim ),( Yang Hoon Nam ),( Dae Hee Choi ),( Chang Don Kang ),( Sung Joon Lee ),( Wan Joo Chun ),( Young Joon Ryu ) 대한장연구학회 2014 Intestinal Research Vol.12 No.3
Background/Aims: Ethanol administration causes intestinal epithelial cell damage by increasing intestinal permeability and the translocation of endotoxins from intestinal bacterial flora. Heat shock proteins (HSPs) are associated with recovery and protection from cell damage. The aim of the current study was to investigate differences in the expression of HSPs in the small intestine and the biochemical changes attributable to ethanol-induced intestinal damage. Methods: Ethanol (20%) was injected intraperitoneally (2.75 g/kg, 5.5 g/kg, 8.25 g/kg) in ICR mice and the same volume of saline was administered to controls. After 1 hour, the proximal, middle, and distal segments were taken from the small intestine and the degree of damage was analyzed. In each segment, the expression of HSPs was analyzed by western blotting. The expression of inflammatory mediators including interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2), and antioxidant enzyme such as glutathione-S-transferase were compared using real-time polymerase chain reaction assays. Results: In the control group, HSP70 increased in all segments of small intestine. Additionally, increases in the expression of HSP40 and HSP90 in the distal regions and an increase in HSP32 in the middle regions were observed. After ethanol treatment, greater histological damage was observed in the distal small intestine and significant decreases in HSPs were observed generally. Increased expression of IL-1β, TNF-α, and COX-2 was observed in small intestinal tissues exposed to ethanol-induced damage. However, there was no significant difference in the expression of an antioxidant enzyme. Conclusions: Significant differences in the expression of HSPs in different intestinal regions were observed. These differences may have been attributable to the distribution of intestinal bacteria. (Intest Res 2014;12:205-213)
Physical and Sensory Properties of Low Fat Sausage Amended with Hydrated Oatmeal and Various Meats
Yang, Han-Sul,Kim, Gap-Don,Choi, Sung-Gil,Joo, Seon-Tea Korean Society for Food Science of Animal Resource 2010 한국축산식품학회지 Vol.30 No.3
Low-fat sausages were prepared with various meats to investigate the effect of the addition of oatmeal at 10% as a fat substitute. The sausages were made with beef, pork and chicken after trimming the visible fat, and the physical and sensory properties of the sausages were evaluated. Beef sausage had the lowest cooking yield and the highest hardness, while chicken sausage showed the opposite properties. The addition of oatmeal resulted in sausage products with less cooking loss and softer texture for all types of meat sausages. Such changes were more pronounced for beef low-fat sausage than for the other types of sausages. The results of moisture absorption suggested that the difference in cooking yield and hardness among sausage products was due to the water-retention properties of different meats and the substitute in response to heat treatment. Sensory evaluation indicated that the greatest overall acceptability of the sausage products were obtained from 10% oatmeal-added pork sausage and that the addition of oatmeal led to better acceptability for all types meat sausages.
( Sung Hoon Moon ),( Kee Don Choi ),( Jin Tae Park ),( Dong Hoon Yang ),( Chang Yun Hwang ),( Ho June Song ),( Hwoon Yong Jung ),( Jin Ho Kim ) 대한소화기학회 2007 SIDDS Vol.9 No.-
Background/Aims: Potential risk of seeding of malignant cells during procedure including submucosal injection is one of our concerns in incomplete endoscopic mucosal resection (EMR). The present study investigated the outcome of gastrectomy after incomplete EMR for early gastric cancer (EGC). Methods: We analyzed 54 consecutive patients with EGC treated surgically after incomplete EMR or gastric wall perforation. EMR was performed between Jan. 1997 and Dec. 2005. We classified the incomplete EMR as the positive lateral margin; positive vertical margin including sub-mucosal invasion; positive both margins; undifferentiated histologic type. After gastrectomy, follow-up consisted of endoscopy and abdominal computed tomography annually. Results: Of the 54 patients, 37 patients were men and 17, women. Median patient age was 63 years (range 34-81 years). Causes of gastrectomy were as follows: 24 cases of positive lateral margin; 20 cases of positive vertical margin; 4 cases of positive both margins; 3 cases of undifferentiated histologic type; 3 cases of gastric wall perforation. Curative resection with LN dissection was performed in 53 patients and one patient underwent wedge resection, 2 patients (3.7%) had LN metastasis, they both underwent gastrectomy for submucosal invasion. There was one case of recurrence after gastrectomy (1.9%). He received EMR for 1.6cm sized moderately differentiated EGC and underwent surgery 2 days later when the result of mucosectomy revealed submucosal invasion. There was no regional LN metastasis out of 11 dissected LN. 5 years 10 months after surgery, hepatic metastasis and lymphadenopathy along heptoduodenal ligament were noted which proved to be metastatic adenocarcinoma by pathology. Despite receiving chemotherapy, be died 21 months after recurrence. Recurrence rate in patients with EGC who had been operated in our hospital between 1990 and 1997 was reported to be 4.1%. Conclusions: This study suggests that incomplete EMR dose not affect the prognosis of early gastric cancer surgery.
Morphological Development of Embryo, Larvae and Juvenile in Yellowtail Kingfish, Seriola lalandi
Sang Geun Yang,Sang Woo Hur,Seung Cheol Ji,Sang Gu Lim,Bong Seok Kim,Minhwan Jeong,Chi Hoon Lee,Young-Don Lee 한국발생생물학회 2016 발생과 생식 Vol.20 No.2
This study monitored the morphological development of embryo, larvae and juvenile yellowtail kingfish, Seriola lalandi, for their aquaculture. The fertilized eggs obtained by natural spawning were spherical shape and buoyant. Fertilized eggs were transparent and had one oil globule in the yolk, with an egg diameter of 1.35 ± 0.04 mm and an oil globule diameter of 0.32 ± 0.02 mm. The fertilized eggs hatched 67–75 h after fertilization in water at 20 ± 0.5°C. The total length (TL) of the hatched larvae was 3.62 ± 0.16 mm. During hatching, the larvae, with their mouth and anus not yet opened. The yolk was completely absorbed 3 days after hatching (DAH), while the TL of post-larvae was 4.72 ± 0.07 mm. At 40 DAH, the juveniles had grown to 30.44 ± 4.07 mm in TL, body depth increased, the body color changed to a black, yellow, and light gray-blue color, and 3–4 vertical stripes appeared. At 45 DAH, the juveniles were 38.67 ± 5.65 mm in TL and 10.10 ± 0.94 mm in body depth. The fish were green with a light orange color, with 7 faint green-brown stripes on the sides of their body. At 87 DAH, the juveniles had grown to 236.11 mm in TL, 217.68 mm in fork length, and 136.5 g in weight. The fish resembled their adult form, with a light yellow-green body color, loss of the pattern on the sides of their body, and a yellow coloration at the tip of the caudal fin.