http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Cheul Ho Lee(이철호),Dae-Jung Kim(김대중),Chang Hwa Jeong(정창화),Gyeong Cheol Choi(최경철),Chae Sung Lee(이채성),Dong Soo Kim(김동수) 한국생명과학회 2010 생명과학회지 Vol.20 No.11
본 연구는 무지개송어 양식 산업의 생산성 향상을 위해 전 암컷 무지개송어 대량생산을 위한 일환으로 성호르몬에 의한 생리학적 성전환과 자성발생 2배체어를 유도하였다. 생리학적으로 성전환된 수컷을 만들기 위하여 부화 후 첫 먹이를 먹는 시기에 웅성호르몬인 17 alpha-methyltestosterone (MT) 5 ㎎을 사료에 흡착시켜 사육수온 13℃에서 적산수온 800℃까지 처리한 결과 96.7%의 수컷 유도율을 보였다. 또한 정상 수컷 정액을 이용하여 수정 10분 후 28oC에서 20분간 고온 처리하여 61.7%의 자성발생 2배체가 유도되었다. This study was conducted to increase the efficiency of farming practice in rainbow trout, Oncorhynchus mykiss, by sex reversal and chromosome-set manipulation techniques. To obtain phenotypic males, hormonal sex reversal was carried out using an exogenous hormone treatment method. 5 ㎎ of 17 alpha-methyltestosterone per kg diet was supplied for 82 days after first feeding at 10℃ and 13℃. More than 93% of the male population was produced by this method and growth of hormone-treated fish at 13℃ was faster than that of untreated bi-sexual groups. Induced diploid gynogenesis was carried out using artificial insemination of UV-irradiated sperm into haploid eggs. Based on the appearance of the rate of haploid syndrome and survival of embryo, a UV ray dose of at least 3,600 erg/㎠ was required to inactivate rainbow trout sperm genetically. Haploid embryos were restored to diploid by blocking the extrusion of the second polar body using heat shock treatment at 28℃ for 20 min, 10 min post insemination. Gynogenetic diploid sex ratios were confirmed after maturation of the fish erythrocyte measurements and chromosome counts.
Cannabidiol-induced apoptosis is mediated by activation of Noxa in human colorectal cancer cells
Jeong, Soyeon,Yun, Hye Kyeong,Jeong, Yoon A,Jo, Min Jee,Kang, Sang Hee,Kim, Jung Lim,Kim, Dae Yeong,Park, Seong Hye,Kim, Bo Ram,Na, Yoo Jin,Lee, Sun Il,Kim, Han Do,Kim, Dae Hyun,Oh, Sang Cheul,Lee, Da Elsevier 2019 Cancer letters Vol.447 No.-
<P><B>Abstract</B></P> <P>Cannabidiol (CBD), one of the compounds present in the marijuana plant, has anti-tumor properties, but its mechanism is not well known. This study aimed to evaluate the apoptotic action of CBD in colorectal cancer (CRC) cells, and focused on its effects on the novel pro-apoptotic Noxa-reactive oxygen species (ROS) signaling pathway. CBD experiments were performed using the CRC cell lines HCT116 and DLD-1. CBD induced apoptosis by regulating many pro- and anti-apoptotic proteins, of which Noxa showed significantly higher expression. To understand the relationship between Noxa and CBD-induced apoptosis, Noxa levels were downregulated using siRNA, and the expression of apoptosis markers decreased. After ROS production was blocked, the level of Noxa also decreased, suggesting that ROS is involved in the regulation of Noxa, which along with ROS is a well-known pro-apoptotic signaling agents. As a result, CBD induced apoptosis in a Noxa-and-ROS-dependent manner. Taken together, the results obtained in this study re-demonstrated the effects of CBD treatment <I>in vivo</I>, thus confirming its role as a novel, reliable anticancer drug.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Our results strongly suggest, for the first time, that CBD can cause Noxa-induced cell death. </LI> <LI> CBD induced apoptotic cell death via ROS/Endoplasmic Reticulum stress-regulated Noxa activation in colorectal cancer cells. </LI> <LI> These results suggest that CBD has important implications for the potential treatment of human CRC. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
제주흑우 집단에서 모색 관련 유전자와 microsatellite marker의 다형현상을 이용한 수정란이식 및 인공수정 유래 후대우 검증
Sang-Hyun Han(한상현),Jin-Cheul Ko(고진철),Young-Hoon Kim(김영훈),Kim Nam-Young(김남영),Kim Jae-Hwan(김재환),Moon-Suck Ko(고문석),Ha-Yeon Jeong(정하연),In-Cheol Cho(조인철),Young-Hoon Yang(양영훈),Sung-Soo Lee(이성수) 한국생명과학회 2010 생명과학회지 Vol.20 No.3
농가에 보급된 제주흑우 수정란이식 및 인공수정 생산축의 확인을 위하여 분자유전학적 실험기법을 이용한 개체 추척을 수행하였다. 유전자 marker 체계는 ISAG 권장 MS marker 11종, 예비시험 후 선발된 SAES marker 11종, 흑모색 관련 MC1R과 ASIP 유전자들을 조합하여 분석하였다. 분석결과 부모 정보가 없는 상태에서의 부권 부정율이 국제권장기준보다 높은 수준을 보였으며, 형매간 동일개체출현률은 5.3×10<SUP>-10</SUP>으로 조사되었다. 친자검정 결과 후보축에 대한 후보 부, 모, 부모 모두가 확인되는 경우는 각각 77.0, 54.0, 40.5%였다. 부-모-자간 trio-mismatch가 전혀 없는 수정란이식 개체는 공급 수정란 대비 14.7%로 확인되었고, 전체 후보축군 중 32.4%는 후보부와의 mismatch가 없는 인공수정에 의해 생산된 개체들로 판정하였다. ISAG marker들만을 분석한 결과에서는 7두가 동일한 3가지 유전자형 조합을 나타내었으나, ISAG/SAES marker들을 조합했을 때에는 2두에서만 동일 유전자형 조합을 나타내었다. MS와 모색유전자 분석자료를 모두 조합했을 때는 조사된 모든 개체들이 서로 구분되었다. 현재의 제주흑우집단이 소수 핵군에서 인공수정과 수정란이식 등 생명공학 기법으로 육성된 집단이기에 제주흑우집단의 유전적 다양성은 낮게 나타났다. 본 연구는 유전자 개체식별과 혈통관리 체계의 구축을 위해서는 적어도 20개 이상의 MS marker와 모색관련 유전자형 자료가 필수적으로 활용되어야함을 제안하고 있으며, 연구결과는 향후 제주흑우의 분자육종에 있어 유용한 자료가 될 것으로 시사하고 있다. To find offspring of Jeju Black cattle (JBC) produced by embryo transfer (ET) and artificial insemination (AI), a molecular genetic study was carried out in candidate cattle populations collected from cattle farms in Jeju Island, Korea. The genetic marker set was composed of 11 ISAG microsatellite (MS) markers, 11 SAES MS markers selected by our preliminary analysis for population diversity of JBC, and two major coat color related genes: MC1R and ASIP. The results showed a combined non-exclusion probability for first parent (NE-P1) that was higher than that recommended by ISAG (above 0.9995), and a combined non-exclusion probability for sib identity of 5.3×10<SUP>-10</SUP>. Parentage analysis showed that the cases identified the candidate’s father only (77.0%), mother only (54.0%), and both parents (40.5%) in the candidate offspring population. The ET and AI calves were identified as 14.7% in the in vitro fertilized eggs provided and 32.4% in total population, respectively. However, the result from ISAG marker analysis showed 3 identical allele-combinations in 7 calves, and that from ISAG/SAES MS marker combination also showed 1 identical allele-combination in 2 calves. Data from MS and coat-color gene analyses provided information for complete identification of all animals tested. Because the present JBC population was mostly bred using small nuclear founders through bioengineering techniques such as AI and ET, the genetic diversity levels obtained from MS analysis in the JBC population were relatively lower than those of other cattle populations, including Hanwoo. The results suggested that the more efficient marker combinations, including coat color related genotypes, should be studied and used for constructing a system for identification and molecular breeding of JBC as well.
Jeong, Mi Jin,Choi, Go Eun,Ghimire, Balkrishna,Lee, Ha Yan,Jung, Ji Young,Choi, Myung Suk,Ku, Ja Jung,Lee, Kyung Mee,Lee, Cheul Ho,Suh, Gang Uk,Son, Sung Won Korean Society of Horticultural Science 2016 원예과학기술지 Vol.34 No.3
In this study, we investigated the effect of seed collection stage and alternating temperature on the germination of Deutzia paniculata (Hydrangeaceae). The seeds were collected from naturally growing D. paniculata populations in Mt. Palgong, Gyeonsangbuk-do, Korea every two weeks over a three month period from August through October, 2014, totaling five collections. We examined seed characteristics such as seed size (length and width) and weight, and germination percentages for each collection stage under four different germination temperature regimes. We also examined germination after 30 and 60 days of cold moist stratification. Each collection phase had a different germination percentage and the lowest germination percentage was observed in the seeds from phase I (average GP of 15.7%). The germination percentage was similar at the later collection phases and in the higher temperatures suggesting that later collection times and higher temperatures are optimal for D. paniculata germination. We found that the optimum collection stage with high viability and germination percentage of for D. paniculata was during the middle of the September.
무지개송어의 자성발생2배체 성전환 수컷을 이용한 전 암컷 생산
Cheul Ho Lee(이철호),Dae-Jung Kim(김대중),Chang Hwa Jeong(정창화),Gyeong Cheol Choi(최경철),Chae Sung Lee(이채성),Dong Soo Kim(김동수) 한국생명과학회 2010 생명과학회지 Vol.20 No.12
본 연구는 무지개송어 양식 산업의 생산성 향상을 위한 일환으로 염색체공학 기법을 이용하여 전 암컷 무지개 송어의 대량생산을 유도 하였다. 자성발생 2배체를 유도한 후 17 alpha-methyltestosteron으로 성전환을 성공적으로 유도하였다. 성전환된 수컷에서는 일반 암컷 모양을 띤 생식소가 형성 되었으나, 수정관의 발달은 보이지 않는 전형적인 성전환 개체의 특징을 나타내었다. 성전환된 자성발생 2배체 가짜수컷을 이용하여 정상 암컷과 단순교배로 발안율 55.7%, 부화율 52.9%의 전 암컷 집단 97,850 마리를 얻을 수 있었고, 4개월 사육 후 치어기(6~7 ㎝) 때의 생식소 확인 결과 100% 암컷임이 확인되었다. This study was conducted to increase the efficiency of farming practice in rainbow trout, Oncorhynchus mykiss, by sex reversal and chromosome-set manipulation techniques. Induction of sex-reversed gynogenetic diploid rainbow trout males and mass production of all-female rainbow trout by genetic sex reversal was performed. Phenotypic males in the gynogenetic diploid group were induced successfully by dietary administration of 5 ㎎ of 17 alpha-methyltestosterone per kg diet for 82 days. All females were produced by crossing between normal female and sex-reversed gynogenetic diploid male rainbow trout.
( Sung Woo Ryu ),( Gene Hyun Bok ),( Jae Young Jang ),( Soung Won Jeong ),( Nam Seok Ham ),( Ji Hye Kim ),( Eui Ju Park ),( Jin Nyoung Kim ),( Woong Cheul Lee ),( Kwang Yeun Shim ),( Sae Hwan Lee ),( 대한소화기학회 2014 Gut and Liver Vol.8 No.3
Background/Aims: To evaluate the diagnostic value of contrast (SonoVue®) enhancement ultrasonography (CEUS) and to compare this method with computed tomography (CT) and magnetic resonance imaging (MRI) in evaluating liver masses. Methods: CEUS (n=50), CT (n=47), and MRI (n=43) were performed on 50 liver masses in 48 patients for baseline mass characterization. The most likely impression for each modality and the final diagnosis, based on the combined biopsy results (n=14), angiography findings (n=36), and clinical course, were determined. The diagnostic value of CEUS was compared to those of CT and MRI. Results: The final diagnosis of the masses was hepatocellular carcinoma (n=43), hemangioma (n=3), benign adenoma (n=2), eosinophilic abscess (n=1), and liver metastasis (n=1). The overall diagnostic agreement with the final diagnosis was substantial for CEUS, CT, and MRI, with κ values of 0.621, 0.763, and 0.784, respectively. The sensitivity, specificity, and accuracy were 83.3%, 87.5%, and 84.0%, respectively, for CEUS; 95.0%, 87.5%, and 93.8%, respectively, for CT; and 94.6%, 83.3%, and 93.0%, respectively for MRI. After excluding the lesions with poor acoustic sonographic windows, the sensitivity, specificity, and accuracy for CEUS were 94.6%, 87.5%, and 93.3%, respectively, with a κ value of 0.765. Conclusions: If an appropriate acoustic window is available, CEUS is comparable to CT and MRI for the diagnosis of liver masses.
김성현 ( Sung Hyun Kim ),박민아 ( Min Ah Park ),이창민 ( Chang Min Lee ),권혁찬 ( Hyuk Chan Kwon ),김재석 ( Jae Seok Kim ),김대철 ( Dae Cheul Kim ),노미숙 ( Mee Suk Roh ),정진숙 ( Jin Suk Jeong ),김민찬 ( Min Chan Kim ),정갑중 ( G 대한내과학회 2004 대한내과학회지 Vol.67 No.1
목적: 위장관 간질 종양 (gastrointestinal stromal tumor, GIST)은 위, 소장, 대장 등에서 발견되는 간엽조직 기원의 종양 중 가장 많이 발생하는 종양으로 병리학적 기준, 악성도의 평가 및 예후인자 등에 관하여 많은 논란이 있다. 면역조직화학적으로 위장관 간질 종양은 kit 양성으로 정의되어진다. 이에 저자들은 기존의 예후인자들을 이용하여 예후군을 나누었을 때 기존의 보고들과 유사성이 있는지 알아보기 위해 본 연구를 시행하였 Background : Gastrointestinal stromal tumors (GIST) are the most common mesenchymal tumors of the gastrointestinal tract. The GISTs are immunohistochemically defined as KIT (CD117) positive tumors. To evaluate the prognostic factors for GISTs, we investig