외과적 보조의 급속상악확장술 : 치험 5예 REPORT OF 5 CASES

박충열,이용욱,송종운,김영운,박홍주,오희균,유선열,이기현,황현식 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.1

Five adults (3 cases of bilateral posterior crossbite, 2 cases of unilateral posterior crossbite) with intermaxillary discrepancy of over 5㎜ due to maxillary transverse deficiency were treated by surgically assisted rapid maxillary expansion (SARME). Bilateral maxillary horizontal osteotomies, osteotomies of pterygomaxillary junction and anterior lateral nasal wall, ans anterior palatal osteotomy were performed in 4 cases, and unilateral osteotomies in 1case, followed by palatal expansion with tooth-borne orthopedic applicance. No significant complication was observed. The mean palatal expansion was 7.2㎜ (5.8∼10.0㎜) in the maxillary first molar region. During the mean follow-up period of 4 years (5 months∼8 years 7 months), no recurrence of crossbite was observed in all cases. The SARME seemed to be a simple, stable, and reliable procedure for achieving a permanent increase of over 5㎜ in skeletal maxillary transverse width of adults.

Validating of the pre-clinical mouse model for metastatic breast cancer to the mandible

HWANG, Young Sun,HAN, Sang-Sun,KIM, Ki-Rim,Ye-Jin, LEE,Sun-Kyung, LEE,Kwang-Kyun, PARK,Won-Yoon, CHUNG Faculdade de Odontologia de Bauru da Universidade 2015 Journal of applied oral science Vol.23 No.1

<P>Metastatic breast carcinoma has a great tendency to spread to the mandible. It is concomitantly associated with bone destruction, food intake disorder, and a poorer prognosis. Appropriate animal models need to be developed for a better understanding of the mechanisms underlying the metastatic process of breast cancer cells to mandible and to test the effects of potential lead compounds. Here, we assessed the metastasis model of intracardiac injection using luciferase-transfected metastatic breast cancer cells (MDA-MB-231<SUP>Luc+</SUP>) by determining the incidences of metastasis, mCT images, and histopathological results. A high bioluminescence signal mainly detected mandibular lesions with less frequent distal femora and proximal tibiae lesions. Extensive mandibular bone destruction occurred in nude mice grafted with metastatic breast cancer cells. This type of animal model might be a useful tool in assessing therapeutic implications and the efficacy of anti-cancer drugs for osteolytic cancers.</P>

저 전압스트레스 및 다채널 전류평형을 위한 Floating 전압 스택형 단일스위치 LED 구동회로

황원선(Won-sun Hwang),류동균(Dong-kyun Ryu),최흥균(Heung-kyun Choi),김희욱(Hugh Kim),한상규(Sang-kyoo Han) 전력전자학회 2014 전력전자학술대회 논문집 Vol.2014 No.7

김치로부터 분리된 Leuconostoc sp. strain YSK 균주에 의한 덱스트란 생산 조건의 최적화

황승균(Seung Kyun Hwang),홍준택(Jun Taek Hong),정경환(Kyung Hwan Jung),장병철(Byung Chul Chang),황경숙(Kyung Suk Hwang),신정희(Jung Hee Shin),임성팔(Sung Paal Yim),유선균(Sun Kyun Yoo) 한국생명과학회 2008 생명과학회지 Vol.18 No.10

본 연구는 발효 김치 액으로부터 덱스트란 생산 균주를 분리하고 생산 최적 생산 조건을 설정하기 위하여 반응표면분석법을 이용하였다. 발효 조건의 독립변수들은 배양 온도, pH, 효모 추출물의 농도, 온도, 기질의 농도로 정하고 Box-Benken 디자인를 이용하여 실험을 설계하였다. 최종 분리된 균주는 Leuconost℃ sp. strain SKY로 잠정적으로 명하였다. 연구 결과 덱스트란 생산은 3.90 - 22.40 g/l이고, 균체량 생산은 0.69-2.85 g/l, 수율은 0.10-0.64, 생산 속도 0.16-0.85 g/l-hr의 범위에서 분석이 되었다. 표면반응분석 결과 덱스트란 생산에 가장 영향을 미치는 것은 배양 pH이고 다음에 효모 추출물의 농도 그리고 온도의 순으로 나타났다. 균체량 생산에 가장 영향을 미치는 것은 배양 pH이고 다음에 효모 추출물의 농도 그리고 온도의 순으로 나타났다. 생산 수율에 가장 영향을 미치는 것은 배양 pH 이고 다음에 효모 추출물의 농도 그리고 온도의 순으로 나타났다. 덱스트란 생산 속도에 가장 영향을 미치는 것은 배양 pH이고 다음에 효모 추출물의 농도 그리고 온도의 순으로 나타났다. 결론적으로 최적 생산 조건은 온도는 27-28℃이고, pH는 7.0이며, 효묘 추출물은 6-7%의 범위에서 결정이 되었다. 이러한 조건에서 생산된 덱스트란양은 22g/l이고, 생산 수율은 약 60%정도이며, 생산 속도는 0.8g/l/hr이었다. A bacterium producing non- or partially digestible dextran was isolated from kimchi broth by enrichment culture technique. The bacterium was identified tentatively as Leuconostoc sp. strain SKY. We established the response surface methodology (Box-Behnken design) to optimize the principle parameters such as culture pH, temperature, and yeast extract concentration for maximizing production of dextran. The ranges of parameters were determined based on prior screening works done at our laboratory and accordingly chosen as 5.5, 6.5, and 7.5 for pH, 25, 30, and 35℃ for temperature, and 1, 5, and 9 g/l yeast extract. Initial concentration of sucrose was 100 g/l. The mineral medium consisted of 3.0 g KH₂PO₄, 0.01 g FeSO₄ㆍH₂O, 0.01 g MnSO₄ㆍ4H₂O, 0.2 g MgSO₄ㆍ7H₂O, 0.01 g NaCl, and 0.05 g CaCO₃ per 1 liter deionized water. The optimum values of pH and temperature, and yeast extract concentration were obtained at pH (around 7.0), temperature (27 to 28℃), and yeast extract (6 to 7 g/l). The best dextran yield was 60% (dextran/g sucrose). The best dextran productivity was 0.8 g/h-l.

EPM 법에 의해 제조된 Ti-46.6Al-1.4Mn-2Mo 합금의 기공 제어

황선근,김종균 대한금속재료학회(대한금속학회) 1998 대한금속·재료학회지 Vol.36 No.9

Formation mechanism and control of pores in a gamma alloy synthesized by elemental powder metallurgy(EPM) was investigated. The chemical composition of the experimental alloy was Ti-46.6Al-1.4Mn-2Mo(at.%) and elemental powders were used as raw materials to synthesize the alloy by hot extrusion. Retained molybdenum was observed in as-extruded alloy, and formation of pores at molybdenum-rich regions was confirmed in heat treated alloy by back scattered electron imaging as well as by energy dispersive spectroscopy, The porosity increased with the average particle size of Mo powder. Depending on the processing condition the density of pores varied. Pores could not be easily detectable in as-extruded condition ; however, subsequent heat treatment brought them out. Pre-extrusion treatment affected the severity of porosity in extruded and heat-treated condition. Extended soaking or partial sintering prior to extrusion was found to be effective for suppressing porosity during the heat treatment to produce a fully lamellar structure.

폐경기 여성에서 ApoE유전자 다형성에 따른 호르몬 대치요법후 혈중 지질 변화

황정규,정필호,황창선,문인걸,조동희,정호연,한기옥,장학철,신현호 한국지질학회 2000 韓國脂質學會誌 Vol.10 No.2

Combined Application of Trichogramma ostriniae and Bacillus thuringiensis for Eco-friendly Control of Plutella xylostella

Hwang, In-Cheon,Park, Chan,Kang, Dong-Kyun,Jin, Na-Young,Jung, Sun-Young,Seo, Mi-Ja,Kim, Jang-Eok,Youn, Young-Nam,Yu, Yong-Man The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.3

Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae), is one of the most destructive insect pests of cruciferous crops. This study is to determine the effect of a combined treatment of Bacillus thuringiensis (Berliner) and Trichogramma ostriniae (Peng and Chen) (Hymenoptera: Trichogrammatidae) applied for an eco-friendly control of Diamondback moth, P. xylostella. Results of the investigation of the resistance ratio of four field populations of P. xylostella to B. thuringiensis shows that Hoengseong populations were highly resistance to the 17.1-fold of B. thuringiensis subsp. aizawai and to the 36.0-fold of B. thuringiensis subsp. kurstaki. B. thuringiensis was proven to give no detrimental effects on adult survival and eggs parasitism of the wasp based on contact toxicity test. Survival rates of P. xylostella were 31.67% and 14.17% on single treatment of T. ostriniae and B. thuringiensis, respectively. But, survival rate of P. xylostella was 1.07% on a combined treatment of egg parasitoid and B. thuringiensis. The combined treatment of both biological control agents significantly decreased the crops damage rate, though none of the biological control agents alone showed any significant control efficacy.

Functional invadopodia formation through stabilization of the PDPN transcript by IMP-3 and cancer-stromal crosstalk for PDPN expression.

Hwang, Young Sun,Xianglan, Zhang,Park, Kwang-Kyun,Chung, Won-Yoon IRL Press] ; Oxford University Press 2012 Carcinogenesis Vol.33 No.11

<P>We previously reported that insulin-like growth factor-II mRNA-binding protein-3 (IMP-3) depletion (IMP-3(δ)) was shown to inhibit invadopodia formation and extracellular matrix degradation capacity in oral squamous cell carcinoma (OSCC) cells. In this study, we found that IMP-3(δ) cells significantly downregulated the podoplanin (PDPN) level, which resulted in a loss of extracellular matrix degradation activity, although invadopodia was still thriving. From RNA in situ hybridization using a digoxigenin-labeled 3'UTR recognition probe of PDPN and reporter assay with 3'UTR of the PDPN gene cloned downstream from the luciferase reporter gene, we revealed that IMP-3 depletion was shown to be downregulated, which most probably lowered PDPN gene expression by reducing mRNA stabilization. In a xenograft model, PDPN depletion was the cause of a decrease in tumor volume and regional infiltration into nearby stroma. Taken together, transforming growth factor beta 1 increased PDPN expression, which potentiated cancer invasion through increased invadopodia formation and extracellular matrix degradation in the low invasive OSCC cell line. Reciprocally, interleukin-1 beta secreted by OSCC cells, stimulated transforming growth factor beta 1 secretion from stromal fibroblasts to induce PDPN expression in OSCC cells. In addition, a retrospective investigation of OSCC patients found that IMP-3 and PDPN expression significantly correlated with lymph node metastasis of OSCC patients. Moreover, co-expression of IMP-3 and PDPN were frequently detected both in primary and lymph nodes metastatic OSCC cells using immunohistochemical dual staining. Thus, the IMP-3-PDPN axis may be a sensitive target molecule in anti-invadopodia therapy for the treatment of metastatic cancers.</P>

Kalopanaxsaponin A Inhibits the Invasion of Human Oral Squamous Cell Carcinoma by Reducing Metalloproteinase-9 mRNA Stability and Protein Trafficking

Hwang, Young Sun,Park, Kwang-Kyun,Chung, Won-Yoon Pharmaceutical Society of Japan 2012 Biological & pharmaceutical bulletin Vol.35 No.3

<P>An inability to control cancer cell invasion and metastasis is the leading cause of death in patients with cancer. The present study was performed to determine the anti-invasive effect of Kalopanaxsaponin A (KPS-A) on matrix metalloproteinase-9 (MMP-9)-meidated invasion in phorbol 12-myristate 13-acetate (PMA)-stimulated human oral squamous cell carcinoma (OSCC) cells and a murine xenograft model of human OSCC. KPS-A, isolated from <I>Kalopanax pictus</I>, inhibited PMA-induced proliferation and invasion as well as PMA-induced MMP-9 expression and secretion at non-cytotoxic doses. KPS-A treatment reduced the stability of PMA-induced MMP-9 mRNA and inhibited the PMA-induced cytoplasmic translocation of HuR. In PMA-treated cells, KPS-A treatment resulted in the intracellular accumulation of MMP-9 and suppressed Ras-associated binding 1A (Rab1A) expression. KPS-A treatment suppressed PMA-induced phosphorylation of extracellular signal regulated kinase (ERK)1/2 and Akt. Furthermore, the oral administration of KPS-A led to substantial inhibition of tumor growth and the expression of proliferating cell nuclear antigen (PCNA), MMP-9, tissue inhibitor of metalloproteinase-1 (TIMP-1), HuR, and Rab1A in the tumor tissues of mice inoculated with YD-10B OSCC cells. Collectively, KPS-A inhibits the invasiveness of oral cancer by reducing HuR-mediated MMP-9 mRNA stability and Rab1A-mediated MMP-9 secretion <I>via</I> ERK1/2 and phosphatidylinositide 3-kinase (PI3K)/Akt. Therefore, KPS-A is a promising anti-invasive agent.</P>

Structural Basis for Carbohydrate Recognition and Anti-inflammatory Modulation by Gastrointestinal Nematode Parasite <i>Toxascaris leonina</i> Galectin

Hwang, Eun Young,Jeong, Mi Suk,Park, Sang Kyun,Ha, Sung Chul,Yu, Hak Sun,Jang, Se Bok American Society for Biochemistry and Molecular Bi 2016 The Journal of biological chemistry Vol.291 No.49

<P>Toxascaris leonina galectin (Tl-gal) is a galectin-9 homologue protein isolated from an adult worm of the canine gastrointestinal nematode parasite, and Tl-gal-vaccinated challenge can inhibit inflammation in inflammatory bowel disease-induced mice. We determined the first X-ray structures of full-length Tl-gal complexes with carbohydrates (lactose, N-acetyllactosamine, lacto-N-tetraose, sialyllactose, and glucose). Bonds were formed on concave surfaces of both carbohydrate recognition domains (CRDs) in Tl-gal. All binding sites were found in the HXXXR and WGXEER motifs. Charged Arg(61)/Arg(196) and Glu(80)/Glu(215) on the conserved motif of Tl-gal N-terminal CRD and C-terminal CRD are critical amino acids for recognizing carbohydrate binding, and the residues can affect protein folding and structure. The polar amino acids His, Asn, and Trp are also important residues for the interaction with carbohydrates through hydrogen bonding. Hemagglutination activities of Tl-gal were inhibited by interactions with carbohydrates and mutations. We found that the mutation of Tl-gal (E80A/E215A) at the carbohydrate binding region induced protein aggregation and could be caused in many diseases. The short linker region between the N-terminal and C-terminal CRDs of Tl-gal was very stable against proteolysis and maintained its biological activity. This structural information is expected to elucidate the carbohydrate recognition mechanism of Tl-gal and improve our understanding of anti-inflammatory mediators and modulators of immune response.</P>