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Meenakshi Sundaram Sri Abirami Saraswathi,Dipak Rana,Subbiah Alwarappan,Shanmugaraj Gowrishankar,Prabu Vijayakumar,Alagumalai Nagendran 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.76 No.-
Poly (ether imide) [PEI] ultrafiltration membranes are coated by polydopamine (PD) and immobilizedwith silver nanoparticles (AgNPs) to improve the permeation, contaminant separation and anti-foulingproperties. The tailored membranes displayed enhanced permeability (97.2 Lm 2 h 1), hydraulicresistance (13.8 kPa/Lm 2 h 1), average roughness (43 nm), contaminant rejection (>97%) with a higherflux recovery ratio (>95%). PEI/PD/Ag membranes showed anti-biofouling property against gramnegative and gram positive bacteria and facilitated the separation of toxic contaminants. The outstandingstability of PD coating and the presence of AgNPs offer effective and safe water separation.
Siddikee, Md. Ashaduzzaman,Sundaram, Subbiah,Chandrasekaran, Murugesan,Kim, Kiyoon,Selvakumar, Gopal,Sa, Tongmin The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.2
Amelioration of salt stress effect on canola seed germination was investigated using 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing plant growth promoting halotolerant bacteria. NaCl at 120 mM concentration reduced canola seed germination by 50 %. The inoculation of ACC deaminase-producing halotolerant Brevibacterium epidermidis RS15 and Bacillus aryabhattai RS341 at 120 mM NaCl significantly increased the seed germination with decreased seed ACC content. Notably, the hydrolytic enzymes activities like amylase, invertase, and protease also increased due to inoculation of RS15 and RS341 compared to uninoculated salt stress imposed germinating canola seeds. Ethylene emission of salt stress exposed eight-day-old canola seedlings was reduced by 35.4 and 41.1 % compared to uninoculated salt stressed control due to respective inoculation of RS341 and RS15. The amelioration of salt stress inhibitory effect on the canola seed germination was attributed to the inoculation of ACC deaminase-producing halotolerant bacteria modulating ethylene emission and inducing hydrolytic enzymes.
Md. Ashaduzzaman Siddikee,Subbiah Sundaram,MURUGESANCHANDRASEKARAN,김기윤,셀바,사동민 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.2
Amelioration of salt stress effect on canola seed germination was investigated using 1-aminocyclopropane- 1-carboxylic acid (ACC) deaminase-producing plant growth promoting halotolerant bacteria. NaCl at 120 mM concentration reduced canola seed germination by 50 %. The inoculation of ACC deaminase-producing halotolerant Brevibacterium epidermidis RS15 and Bacillus aryabhattai RS341 at 120 mM NaCl significantly increased the seed germination with decreased seed ACC content. Notably, the hydrolytic enzymes activities like amylase, invertase, and protease also increased due to inoculation of RS15 and RS341 compared to uninoculated salt stress imposed germinating canola seeds. Ethylene emission of salt stress exposed eight-day-old canola seedlings was reduced by 35.4 and 41.1 % compared to uninoculated salt stressed control due to respective inoculation of RS341 and RS15. The amelioration of salt stress inhibitory effect on the canola seed germination was attributed to the inoculation of ACC deaminase-producing halotolerant bacteria modulating ethylene emission and inducing hydrolytic enzymes.
Shagol, Charlotte C,Krishnamoorthy, Ramasamy,Kim, Kiyoon,Sundaram, Subbiah,Sa, Tongmin Ecomed 2014 Environmental Science and Pollution Research Vol.21 No.15
<P>The Janghang smelter in Chungnam, South Korea started in 1936 was subsequently shutdown in 1989 due to heavy metal (loid) pollution concerns in the vicinity. Thus, there is a need for the soil in the area to be remediated to make it usable again especially for agricultural purposes. The present study was conducted to exploit the potential of arsenic (As)-tolerant bacteria thriving in the vicinity of the smelter-polluted soils to enhance phytoremediation of hazardous As. We studied the genetic and taxonomic diversity of 21 As-tolerant bacteria isolated from soils nearer to and away from the smelter. These isolates belonging to the genera Brevibacterium, Pseudomonas, Microbacterium, Rhodococcus, Rahnella, and Paenibacillus, could tolerate high concentrations of arsenite (As(III)) and arsenate (As(V)) with the minimum inhibitory concentration ranging from 3 to >20?mM for NaAsO2 and 140 to 310?mM NaH2AsO4??7H2O, respectively. All isolates exhibited As(V) reduction except Pseudomonas koreensis JS123, which exhibited both oxidation and reduction of As. Moreover, all the 21 isolates produced indole acetic acid (IAA), 13 isolates exhibited 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, 12 produced siderophore, 17 solubilized phosphate, and 13 were putative nitrogen fixers under in vitro conditions. Particularly, Rhodococcus aetherivorans JS2210, P. koreensis JS2214, and Pseudomonas sp. JS238 consistently increased root length of maize in the presence of 100 and 200?μM As(V). Possible utilization of these As-tolerant plant-growth-promoting bacteria can be a potential strategy in increasing the efficiency of phytoremediation in As-polluted soils.</P>
Leifsonia soli sp. nov., a yellow-pigmented actinobacterium isolated from teak rhizosphere soil
Madhaiyan, Munusamy,Poonguzhali, Selvaraj,Lee, Jung-Sook,Senthilkumar, Murugaiyan,Lee, Keun Chul,Sundaram, Subbiah Microbiology Society 2010 International journal of systematic and evolutiona Vol.60 No.6
<P>Two yellow-pigmented, Gram-stain-positive, aerobic, motile, short rod-shaped bacteria were isolated from natural teak tree rhizosphere soil and their taxonomic positions were determined by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strains TG-S248<SUP>T</SUP> and TG-S240 formed a distinct phyletic line within the genus <I>Leifsonia</I>. 16S rRNA gene sequence analysis of strain TG-S248<SUP>T</SUP> with sequences from <I>Leifsonia shinshuensis</I> DB 102<SUP>T</SUP>, <I>L. poae</I> VKM Ac-1401<SUP>T</SUP>, <I>L. naganoensis</I> DB 103<SUP>T</SUP>, <I>L. aquatica</I> DSM 20146<SUP>T</SUP> and <I>L. xyli</I> subsp. <I>cynodontis</I> JCM 9733<SUP>T</SUP> revealed pairwise similarities ranging from 98.7 to 99.1 %. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The G+C content of the DNA of the type strain was 69.4 mol%. DNA-DNA hybridization experiments revealed low levels of DNA-DNA relatedness (32 % or less) between strain TG-S248<SUP>T</SUP> and its closest relatives. Based on differences in phenotypic and genotypic characteristics, strain TG-S248<SUP>T</SUP> (=LMG 24767<SUP>T</SUP> =JCM 15679<SUP>T</SUP>) is designated the type strain of a novel species of the genus <I>Leifsonia</I>, for which the name <I>Leifsonia soli</I> sp. nov. is proposed.</P>
Madhaiyan, Munusamy,Poonguzhali, Selvaraj,Lee, Jung-Sook,Lee, Keun Chul,Sundaram, Subbiah Microbiology Society 2010 International journal of systematic and evolutiona Vol.60 No.9
<P>An aerobic, yellow-pigmented, facultatively methylotrophic, Gram-staining-negative, non-spore-forming bacterium, designated strain Gm-149<SUP>T</SUP>, was isolated from the rhizosphere of cultivated soybean in India. Cells were motile by gliding. The predominant cellular fatty acids were iso-C15 : 0, summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1<I>ω</I>7<I>c</I>), C16 : 0 3-OH and anteiso-C15 : 0, and the major isoprenoid quinone was MK-6. The G+C content of the genomic DNA of strain Gm-149<SUP>T</SUP> was 35.6 mol%. Comparative 16S rRNA gene sequence analysis showed that strain Gm-149<SUP>T</SUP> formed a distinct phyletic line within the genus <I>Flavobacterium</I>. Based on levels of pairwise 16S rRNA gene sequence similarity, strain Gm-149<SUP>T</SUP> was related most closely to the type strain of <I>Flavobacterium daejeonense</I> (97.1 %), but the level of DNA-DNA relatedness between these two strains was about 11.2 %. On the basis of phenotypic and genotypic data, strain Gm-149<SUP>T</SUP> is considered to represent a novel species of the genus <I>Flavobacterium</I>, for which the name <I>Flavobacterium glycines</I> sp. nov. is proposed. The type strain is Gm-149<SUP>T</SUP> (=ICMP 17618<SUP>T</SUP>=NBRC 105008<SUP>T</SUP>).</P>