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Liu, Suli,Im, Hogune,Bairoch, Amos,Cristofanilli, Massimo,Chen, Rui,Deutsch, Eric W.,Dalton, Stephen,Fenyo, David,Fanayan, Susan,Gates, Chris,Gaudet, Pascale,Hincapie, Marina,Hanash, Samir,Kim, Hoguen American Chemical Society 2013 JOURNAL OF PROTEOME RESEARCH Vol.12 No.1
<P>We report progress assembling the parts list for chromosome 17 and illustrate the various processes that we have developed to integrate available data from diverse genomic and proteomic knowledge bases. As primary resources, we have used GPMDB, neXtProt, PeptideAtlas, Human Protein Atlas (HPA), and GeneCards. All sites share the common resource of Ensembl for the genome modeling information. We have defined the chromosome 17 parts list with the following information: 1169 protein-coding genes, the numbers of proteins confidently identified by various experimental approaches as documented in GPMDB, neXtProt, PeptideAtlas, and HPA, examples of typical data sets obtained by RNASeq and proteomic studies of epithelial derived tumor cell lines (disease proteome) and a normal proteome (peripheral mononuclear cells), reported evidence of post-translational modifications, and examples of alternative splice variants (ASVs). We have constructed a list of the 59 “missing” proteins as well as 201 proteins that have inconclusive mass spectrometric (MS) identifications. In this report we have defined a process to establish a baseline for the incorporation of new evidence on protein identification and characterization as well as related information from transcriptome analyses. This initial list of “missing” proteins that will guide the selection of appropriate samples for discovery studies as well as antibody reagents. Also we have illustrated the significant diversity of protein variants (including post-translational modifications, PTMs) using regions on chromosome 17 that contain important oncogenes. We emphasize the need for mandated deposition of proteomics data in public databases, the further development of improved PTM, ASV, and single nucleotide variant (SNV) databases, and the construction of Web sites that can integrate and regularly update such information. In addition, we describe the distribution of both clustered and scattered sets of protein families on the chromosome. Since chromosome 17 is rich in cancer-associated genes, we have focused the clustering of cancer-associated genes in such genomic regions and have used the ERBB2 amplicon as an example of the value of a proteogenomic approach in which one integrates transcriptomic with proteomic information and captures evidence of coexpression through coordinated regulation.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jprobs/2013/jprobs.2013.12.issue-1/pr300985j/production/images/medium/pr-2012-00985j_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/pr300985j'>ACS Electronic Supporting Info</A></P>
CCQM K6.2 determination of total cholesterol in human serum
Wise, Stephen A,Phinney, Karen W,Duewer, David L,Sniegoski, Lorna T,Welch, Michael J,Pabello, Guiomar,Caldero, Marco A Avila,Qinde, Liu,Kooi, Lee Tong,Rego, Eliane,Garrido, Bruno,Allegri, Gabriella,Cr BUREAU INTERNATIONAL DES POIDS ET MESURES 2018 METROLOGIA -BERLIN- Vol.55 No.1
Lin, Kaili,Liu, Bin,Lim, Sze-Lam,Fu, Xiuqiong,Sze, Stephen C.W.,Yung, Ken K.L.,Zhang, Shiqing The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.3
Background: Active natural ingredients, especially small molecules, have recently received wide attention as modifiers used to treat neurodegenerative disease by promoting neurogenic regeneration of neural stem cell (NSC) in situ. 20(S)-protopanaxadiol (PPD), one of the bioactive ingredients in ginseng, possesses neuroprotective properties. However, the effect of PPD on NSC proliferation and differentiation and its mechanism of action are incompletely understood. Methods: In this study, we investigated the impact of PPD on NSC proliferation and neuronal lineage differentiation through activation of the Wnt/glycogen synthase kinase (GSK)-3β/β-catenin pathway. NSC migration and proliferation were investigated by neurosphere assay, Cell Counting Kit-8 assay, and EdU assay. NSC differentiation was analyzed by Western blot and immunofluorescence staining. Involvement of the Wnt/GSK3β/β-catenin pathway was examined by molecular simulation and Western blot and verified using gene transfection. Results: PPD significantly promoted neural migration and induced a significant increase in NSC proliferation in a time- and dose-dependent manner. Furthermore, a remarkable increase in anti-microtubule-associated protein 2 expression and decrease in nestin protein expression were induced by PPD. During the differentiation process, PPD targeted and stimulated the phosphorylation of GSK-3β at Ser9 and the active forms of β-catenin, resulting in activation of the Wnt/GSK-3β/β-catenin pathway. Transfection of NSCs with a constitutively active GSK-3β mutant at S9A significantly hampered the proliferation and neural differentiation mediated by PPD. Conclusion: PPD promotes NSC proliferation and neural differentiation in vitro via activation of the Wnt/GSK-3β/β-catenin pathway by targeting GSK-3β, potentially having great significance for the treatment of neurodegenerative diseases.
The flexural performance of laminated glass beams under elevated temperature
Huang, Xiaokun,Liu, Gang,Liu, Qiang,Bennison, Stephen J. Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.52 No.3
A series of experimental work is carried out with the aim to understand the flexural performance of laminated glass (LG) beams using polyvinyl butyral (PVB) and Ionoplast interlayers subjected to short term duration loads in the circumstance of elevated temperature. The study is based on a total of 42 laboratory tests conducted in ambient temperature ranging from $25^{\circ}C$ to $80^{\circ}C$. The load duration is kept within 20 seconds. Through the tests, load-stress and load-deflection curves of the LG are established; appropriate analytical models for the LG are indentified; the effective thicknesses as well as the shear transfer coefficients of the LG are semi-empirically determined. The test results show that within the studied temperature range the bending stresses and deflections at mid-span of the LG develop linearly with respect to the applied loads. From $25^{\circ}C$ to $80^{\circ}C$ the flexural behavior of the PVB LG is found constantly between that of monolithic glass and layered glass having the same nominal thickness; the flexural behavior of the Ionoplast LG is equivalent to monolithic glass of the same nominal thickness until the temperature elevates up to $50^{\circ}C$. The test results reveal that in calculating the effective thicknesses of the PVB and Ionoplast LG, neglecting the shear capacities of the interlayers is uneconomic even when the ambient temperature is as high as $80^{\circ}C$. In the particular case of this study, the shear transfer coefficient of the PVB interlayer is found in a range from 0.62 to 0.14 while that of the Ionoplast interlayer is found in a range from 1.00 to 0.56 when the ambient temperature varies from $25^{\circ}C$ to $80^{\circ}C$.
An experimental study on the flexural performance of laminated glass
Huang, Xiaokun,Liu, Gang,Liu, Qiang,Bennison, Stephen J. Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.49 No.2
This paper reported an experimental study on creep behaviors of PVB and Ionoplast laminated glass (LG) under load duration of 30 days. The tests were carried out in room temperature ($23^{\circ}C$). The study revealed that after sustaining loads for 30 days, the mid-span deflection of PVB LG increased by almost 102% compared with its short term deflection, while that of Ionoplast LG approximately increased by 14%; composite effects between two glass plies in PVB LG gradually reduced with time, but did not fully vanish at the 30th day; two glass plies in Ionoplast LG on the other hand was able to withstand loads as an effective composite section during the entire loading period; the creep behaviors of both LG were not finished yet at the 30th day. In addition to this, also studied was the varying of the bending stresses of PVB and Ionoplast LG under load duration of 2 hours. The tests were carried out in ambient temperatures of $30^{\circ}C$, $50^{\circ}C$ and $80^{\circ}C$ respectively. It was found that under a given load, although the bending stresses of both LG increased with increasing temperature, for PVB LG the increasing rate of the bending stress decreased with increasing temperature, while for Ionoplast LG the increasing rate of the bending stress increased with increasing temperature.
Kaili Lin,Stephen Cho-Wing Sze,Bin Liu,Zhang Zhang,Zhu Zhang,Peili Zhu,Ying Wang,Qiudi Deng,Ken Kin-Lam Yung,Shiqing Zhang 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.2
Background: Alzheimer"s disease (AD) is one of the most prevalent neurodegenerative disorders. Enhancing hippocampal neurogenesis by promoting proliferation and differentiation of neural stem cells (NSCs) is a promising therapeutic strategy for AD. 20(S)-protopanaxadiol (PPD) and oleanolic acid (OA) are small, bioactive compounds found in ginseng that can promote NSC proliferation and neural differentiation in vitro. However, it is currently unknown whether PPD or OA can attenuate cognitive deficits by enhancing hippocampal neurogenesis in vivo in a transgenic APP/PS1 AD mouse model. Here, we administered PPD or OA to APP/PS1 mice and monitored the effects on cognition and hippocampal neurogenesis. Methods: We used the Morris water maze, Y maze, and open field tests to compare the cognitive capacities of treated and untreated APP/PS1 mice. We investigated hippocampal neurogenesis using Nissl staining and BrdU/NeuN double labeling. NSC proliferation was quantified by Sox2 labeling of the hippocampal dentate gyrus. We used western blotting to determine the effects of PPD and OA on Wnt/GSK3β/β-catenin pathway activation in the hippocampus. Results: Both PPD and OA significantly ameliorated the cognitive impairments observed in untreated APP/PS1 mice. Furthermore, PPD and OA significantly promoted hippocampal neurogenesis and NSC proliferation. At the mechanistic level, PPD and OA treatments resulted in Wnt/GSK-3β/β-catenin pathway activation in the hippocampus. Conclusion: PPD and OA ameliorate cognitive deficits in APP/PS1 mice by enhancing hippocampal neurogenesis, achieved by stimulating the Wnt/GSK-3β/β-catenin pathway. As such, PPD and OA are promising novel therapeutic agents for the treatment of AD and other neurodegenerative diseases.
The flexural performance of laminated glass beams under elevated temperature
Xiaokun Huang,Gang Liu,Qiang Liu,Stephen J. Bennison 국제구조공학회 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.52 No.3
A series of experimental work is carried out with the aim to understand the flexural performance of laminated glass (LG) beams using polyvinyl butyral (PVB) and Ionoplast interlayers subjected to short term duration loads in the circumstance of elevated temperature. The study is based on a total of 42 laboratory tests conducted in ambient temperature ranging from 25°C to 80°C. The load duration is kept within 20 seconds. Through the tests, load-stress and load-deflection curves of the LG are established; appropriate analytical models for the LG are indentified; the effective thicknesses as well as the shear transfer coefficients of the LG are semi-empirically determined. The test results show that within the studied temperature range the bending stresses and deflections at mid-span of the LG develop linearly with respect to the applied loads. From 25°C to 80°C the flexural behavior of the PVB LG is found constantly between that of monolithic glass and layered glass having the same nominal thickness; the flexural behavior of the Ionoplast LG is equivalent to monolithic glass of the same nominal thickness until the temperature elevates up to 50°C. The test results reveal that in calculating the effective thicknesses of the PVB and Ionoplast LG, neglecting the shear capacities of the interlayers is uneconomic even when the ambient temperature is as high as 80°C. In the particular case of this study, the shear transfer coefficient of the PVB interlayer is found in a range from 0.62 to 0.14 while that of the Ionoplast interlayer is found in a range from 1.00 to 0.56 when the ambient temperature varies from 25°C to 80°C.