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중국어 동사 전 목적명사구 및 수동형태소 명사구의 예외적 양태와 그 함축의미
송홍기(Sohng Hong-Ki) 한국생성문법학회 2005 생성문법연구 Vol.15 No.3
It is a well-known linguistic fact that in general, only a subject can serve as an antecedent and a blocker in the case of Chinese monomorphemic reflexivization. Contrary to our expectation, a DP that follows the passive morpheme bei or the preverbal object marker, which is a non-subject, serves as an antecedent for the Chinese reflexive, even though not serving as a blocker for a higher antecedent. Sohng (2004a) shows that the blocking effect for the Chinese monomorphemic reflexive is accounted for in terms of LF Spec-head agreement that assigns and checks the φ-features of ziji. Based on LF head movement of a monomorphemic reflexive and checking of a [Refl] feature in Agr, the analysis put forward in this paper shows clearly that the unexpected non-subject antecedence on ziji is well explained in the split-I phrase structure originating with Pollock(1989) in terms of the Conditions on Subject Orientation and LF Spec-head agreement. Thus, the non-subject antecedence on ziji that this paper deals with provides firm support to the analysis given in this paper.
Glycosylation of free sterol by whole-cell bioconversion in E. coli
Jae Kyung Sohng,Jae Kyung Sohng 한국당과학회 2011 한국당과학회 학술대회 Vol.2011 No.1
Steryl glucosides play important roles in many physiological and biochemical process in organism such as the heat shock, enhancement of immunological system, etc. The alignment of a putative sterol glucosides isolated from S. tropica CNB-440 has been shown 34%, 42% and 57% in homology with the corresponding ones from Arabidopis thaliana, Avena sativa and Salinispora arenicola CNS-205, respectively. Engineered E. coli host-high level production of the UDP-glucose was used for whole-cell bioconversion of free sterol (cholesterol and β-sistosterol) and the production of glycosylated product was only detected with β-sitosterol as substrate.
Sohng, Jae-Kyung,Yoo, Jin-Cheol Korean Society for Biochemistry and Molecular Biol 1996 Journal of biochemistry and molecular biology Vol.29 No.3
DNA fragments, homologous to the dTDP-D-glucose 4,6-dehydratase gene, obtained from the genomic DNA of Streptomyces antibioticus $T\ddot{u}99$, a producer of the unusual macrolide antibiotic chlorothricin, were cloned and sequenced. This dehydratase gene was designated as oxil. The coding region of the oxil gene is composed of 987 bp, and analysis of the DNA sequence data reveals sequences for the gene products of 329 amino acids (molecular weight of 36,037). The deduced amino acids are 59% identical to the StrE, dTDP-D-glucose 4,6-dehydratase from the streptomycin pathway. The oxil's function was examined by expressing it in E. coli using the T7 RNA polymerase/promoter system (pRSET) to produce an active fusion protein including a his tag. This enzyme shows specificity of substrate, specific only to dTDP-D-glucose.
Gene Cloning and Analysis of Biosynthetic Gene of Spectinomycin
Sohng, Jae-Kyung,Oh, Tae-Jin,Liou, Kwang-Kyoung,Lee, Hei-Chan 선문대학교 첨단과학기술연구소 2001 첨단과학기술연구소 논문집 Vol.5 No.-
A biosynthetic gene cluster involved in the production of spectinomyein was cloned from Streptomyes spectabilis cosmid library with dTDP-glucose 4,6-dehydratase and amnino transferase gene probes. The DNA probes were obtained from S. speciabilis genome with primers based on consensus sequences of known enzymes using a polymerase chain reaction. One of the cloned cosmids, pSPM8, contained the homologue of the dTDP-glucose 4,6-dehydratase and amino transferase gene. The DNA sequence analysis reveals four open reading frames and one incomplete reading frame. The putative orf1 protein reveals 29% amino acid sequence homology of putative myo-inositol-2-dehydrogenase from Vibrio anguillamm. The orf2 is about 46% homologous to putative myo-inositol-1-monophosphotase of Mycobacterium leprae. The orf3 showed strong homology (67.4%) to putative export protein (spcT) from s. flavopersicus. The amino acid sequel deduced from orf4 showed a high similarity (70.8%) to a spectinomycin resistance gene (spcN) from S. flavopersicus. Expression of orf4 in E. coli BL21 showed the spectinomycin resistance.
Sohng, Hee Yon,Kuniyuki, Alan,Edelson, Jane,Weir, Rosy Chang,Song, Hui,Tu, Shin-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12
Background: Understanding and enhancing change capabilities, including Practice Adaptive Reserve (PAR), of Community Health Centers (CHCs) may mitigate cancer-related health disparities. Materials and Methods: Using stratified random sampling, we recruited 232 staff from seven CHCs serving Asian Pacific Islander communities to complete a self-administered survey. We performed multilevel regression analyses to examine PAR composite scores by CHC, position type, and number of years worked at their clinic. Results: The mean PAR score was 0.7 (s.d. 0.14). Higher scores were associated with a greater perceived likelihood that clinic staff would participate in an evidence-based intervention (EBI). Constructs such as communication, clinic flow, sensemaking, change valence, and resource availability were positively associated with EBI implementation or trended toward significance. Conclusions: PAR scores are positively associated with perceived likelihood of clinic staff participation in cancer screening EBI. Future research is needed to determine PAR levels most conducive to implementing change and to developing interventions that enhance Adaptive Reserve.
Sohng, Jae Kyung,Yoo, Jin-cheol 朝鮮大學校 1997 藥學硏究誌 Vol.18 No.2
DNA fragments, homologous to the dTDP-Dgiucose 4,6-dehydratase gene, obtained from the genomic DNA of Streptomyces antibioticus Tu¨99,a producer of the unusual macrolide antibiotic chlorothricin, were cloned and aequenced. This dehydratase gene was designated as oxil. The coding region of the oxil gene is composed of 987 bp, and analysis of the DNA sequence data reveals sequences for the gene products of 329 amino acids(molecular weight of 36.037). The deduced amino acids are 59% identical to the StrE, dTDP-D-glucose 4,6-dehydratase from the Streptomycin pathway. The oxil's function was examined by expressing it in E. coil using the T7 RNA polymerase/promoter system(pRSET) to produce an active fusion protein including a his tag. This enzyme shows apecificity of substrate, specific only to dTDP-D-glucose.
Production, Isolation and Biological Activity of Nargenicin from Nocardia sp. CS682
Sohng, Jae-Kyung,Yamaguchi, Tokutaro,Seong, Chi-Nam,Baik, Keun-Sik,Park, Seong-Chan,Lee, Hyo-Jeong,Jang, So-Young,Simkhada, Jaya Ram,Yoo, Jin-Cheol 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.10
Culture broth of an actinomycete isolate, Nocardia sp. CS682 showed specifically higher antibacterial activity against methicilin resistant Staphylococcus aureus (MRSA). Purified substance from the organism, CS-682, which is active against MRSA and Micrococcus leuteus, is a $C_{28}H_{37}NO_8$ ($M+H^+$, observed: 516.83) and identified as an unusual macrolide antibiotic, nargenicin. The chemical structure of CS-682 was identified by FT-IR, $^1H$-NMR, $^{13}C$-NMR, and ($^1H-^1H$ and $^1H-^{13}H$) COSY. The anti-MRSA activity of CS-682 was stronger than that of oxacillin, vancomycin, monensin, erythromycin, and spiramycin. Phylogenetic analysis showed that strain CS682 is closely related to Nocardia tenerifensis DSM $44704^T$ (98.7% sequence similarity), followed by N. brasiliensis ATCC $19296^T$ (98.4% sequence similarity). The ability of Nocardia sp. CS682 to produce nargenicin was unique.