ST6Gal-I Predicts Postoperative Clinical Outcome for Patients with Localized Clear-cell Renal Cell Carcinoma

Liu, Hai-Ou,Wu, Qian,Liu, Wei-Si,Liu, Yi-Dong,Fu, Qiang,Zhang, Wei-Juan,Xu, Le,Xu, Jie-Jie Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Hyperactivated ${\alpha}2$-6-sialylation on N-glycans due to overexpression of the Golgi enzyme ${\beta}$-galactoside: ${\alpha}2$-6-sialyltransferase (ST6Gal-I) often correlates with cancer progression, metastasis, and poor prognosis. This study was aimed to determine the association between ST6Gal-I expression and the risk of recurrence and survival of patients with localized clear-cell renal cell carcinoma (ccRCC) following surgery. We retrospectively enrolled 391 patients (265 in training cohort and 126 in validation cohort) with localized ccRCC underwent nephrectomy at a single center. Tissue microarrays were constructed for immunostaining of ST6Gal-I. Prognostic value and clinical outcomes were evaluated. High ST6Gal-I expression was associated with Fuhrman grade (p<0.001 and p=0.016, respectively) and the University of California Los-Angeles Integrated Staging System (UISS) score (p=0.004 and p=0.017, respectively) in both cohorts. Patients with high ST6Gal-I expression had significantly worse overall survival (OS) (p<0.001 and p<0.001, respectively) and recurrence free survival (RFS) (p<0.001 and p=0.002, respectively) than those with low expression in both cohorts. On multivariate analysis, ST6Gal-I expression remained associated with OS and RFS even after adjusting for the UISS score. Stratified analysis suggested that the association is more pronounced among patients with low and intermediate-risk disease defined by the UISS score. High ST6Gal-I expression is a potential independent adverse predictor of survival and recurrence in ccRCC patients, and the prognostic value is most prominent in those with low and intermediate-risk disease defined by the UISS score.

중국 고대 건축의 회화와 그 전통 환경미학적 개념

유사기 ( Liu Si Jie ),루안샤오인 ( Ruan Xiao Yin ) 한국기초조형학회 2019 한국기초조형학회 학술발표논문집 Vol.2019 No.2

In ancient Chinese architectural painting, which consists of ruler painting (Jiehua), landscape painting (Shanshuihua), and wall painting (Bihua), a good relationship between human, architecture and nature is remained. It is an artistic expression of the most ideal dwelling environment in ancient China. The environmental aesthetics of the architectural painting of ancient China lies in its ecology and culture. The beauty of nature, dwelling, and life constitute the gist of ecological features of the architectural painting of ancient China, while the artistic conception of fairyland image, landscape architecture image, and Feng-shui is the main content of cultural characteristics of which. The significance of environmental aesthetics of the ancient Chinese architectural painting is the creation of artistic conception of perfect dwelling, the reflection of the harmony and unity of human, architecture and nature, as well as the influence of ancient Chinese aesthetics on architecture. Architecture has been regularly appeared in ruler painting, landscape painting and wall painting of ancient Chinese painting. Although ruler paintingand landscape painting were considered originating from the same kind of painting, however, the artistic expression varied from one to another. Until the Five Dynastiesand Song Dynasty, some great masters of ruler painting, like Wei Xian, Guo Zhongshu, Zhang Zeduan, etc, appeared and ruler painting came into mature period.

<신세기 에반게리온>에서 나타난 이데올로기 특징 분석

유사첩(Liu, Si-jie),이종한(Lee, Jong-han) 한국만화애니메이션학회 2017 만화애니메이션연구 Vol.- No.47

1995년 TV시리즈를 통해 등장한 애니메이션 <에반게리온>은 지금도 이데올로 기나 인간의 심리를 반영한 성공적인 애니메이션 작품으로 평가 받고 있다. <신세기 에반게리온>은 일본에서 얻은 폭발적 반응에 힘입어 전 세계 애니메이션계를 강타하였다. 에반게리온의 특징이라면 신선한 메카닉 디자인과 다양하고 사실적인 밀리터리 미장센, 성서를 기반으로 한 심오하고 철학적인 세계관으로 요약할 수 있다. 어느 작품이든 작가의 철학과 이데올로기가 반영되기 마련이지만 <에반게리온>은 특히 여러 행태의 다양한 이데올로기가 반영된 작품이라고 할 수 있다. 또한 <에반게리온>은 제작진이 의도한 난해하고 끝이 모호한 줄거리로 팬들로부터 많은 비판을 받았던 만큼 그에 대한 활발한 토론과 해석을 유도하여 오히려 제2차 홍보의 기능을 하기도 하였다. 이와 같이 작품 속에 나타나는 심오하고 복잡한 이데올로기는 종종 사람들의 관심을 이끌어 흥행의 성공 요인이 되기도 한다. 이 논문에서는 <에반게리온>에서 반영된 이데올로기의 분석을 통해서 이데올로기가 <에반게리온>에 어떤 영향을 주었는지 살펴보고 또 인간 사회에 보편적으로 존재하고 있는 이데올로기가 이 작품에서 어떤 형태로 표현되었는지 이해해 본다. <에반게리온>에서 나타나고 있는 종교, 심리학, 철학의 이데올로기를 분석함으로써 만화애니메이션 작품의 여러 흥행조건 중에서 독자들의 관심을 이끌 수 있는 적절한 이데올리기 역시 흥행의 한 요인이 될 수 있음을 시사하려고 한다. <Evangelion> which was first shown as TV series in 1995 has been highly praised until now successful animation that reflected human psychology and ideology. <Neon Genesis Evangelion> first gained popularity in Japan and spreaded into global animation market. Characteristics of <Evangelion> can be summarized as mechanical design, realistic military mise-en-scene, and philosophical view of the world based on the bible. All works reflect the philosophy and ideology of the writer/creator, but <Evangelion> reflects various form of ideology. Furthermore, <Evangelion>"s ambiguous and vague plot made the fans to discuss and analyze about the work, which brought secondary marketing effect. In this report, the ideology reflected in <Evangelion> will be analyzed and understand how these ideologies were reflected in the work. By analyzing religious, psychological andphilosophy ideologies in <Evangelion>, ideology can be proved as success factor of animation films.

Bacillus oceani sp. nov., a new slightly halophilic bacterium, isolated from a deep sea sediment environment

Liu, Yu-Juan,Long, Li-Juan,Huang, Xiao-Fang,You, Zhi-Qing,Wang, Fa-Zuo,Li, Jie,Kim, Chang-Jin,Tian, Xin-Peng,Zhang, Si Springer-Verlag 2013 Antonie van Leeuwenhoek Vol.104 No.5

Highly Uniform Core/Shell Structured CoFe2O4/MIL-100(Fe) Nanofibers for Efficient Visible Light Photocatalysis

Jun Liu,Meng Sun,Meng-Jie Chang,Si-Meng Fan,Qian Hui,Fu-Rong Ni,Bin Yuan 한국섬유공학회 2021 Fibers and polymers Vol.22 No.2

Novel CoFe2O4/MIL-100(Fe) (CFO/MIL-100(Fe)) core/shell nanofibers were prepared by successive ionic layeradsorption and reaction (SILAR) assembly of MIL-100(Fe) on electrospun CoFe2O4 nanofibers. The MIL-100(Fe) shellswere constructed on the dispersed CoFe2O4 fiber surface with high uniformity, homogeneous thickness and high stability. Due to the inherent porosity of MIL-100(Fe), a higher specific surface area of 225.77 m2/g is achieved for the CFO/MIL-100(Fe)-10 sample. The formed CFO/MIL-100(Fe) catalysts exhibit high catalytic activity towards degradation of rhodamineB (RhB) under visible light irradiation. The photocatalytic activity remains above 77 % within 60 min illumination after fourcycles. Due to the simple synthetic method, highly uniform structure, strong magnetic recoverable, as well as goodphotocatalytic performance, the obtained CFO/MIL-100(Fe) core/shell nanofibers could be promising photocatalysts forpractical application.

CbCOR15, A Cold-Regulated Gene from Alpine Chorispora bungeana, Confers Cold Tolerance in Transgenic Tobacco

Jing Si,Jian-hui Wang,Li-jing Zhang,Hua Zhang,Ya-jie Liu,Li-zhe An 한국식물학회 2009 Journal of Plant Biology Vol.52 No.6

Rapid amplification of cDNA ends was performed to isolate cold-regulated CbCOR15 (EF208112) from Chorispora bungeana. This alpine species is distributed in subnival areas. Transcripts were detected in the leaves, but not the roots, of plants acclimated to cold temperatures. Expression was induced at high levels at both 4°C and −4°C. In comparing its deduced protein sequence to that of AtCOR15a (cold-regulated 15a in Arabidopsis thaliana), the N terminus had less homology than the C terminus while still containing a region analogous to the chloroplast-targeted signal peptide of AtCOR15a. We also introduced CbCOR15, with the CaMV 35S promoter, into tobacco. Second-generation (T1) plants had significantly increased tolerance to chilling, as determined by their electrolyte leakage, chlorophyll content, and relative water content. Further freezing-stress experiments showed that the tolerance of transgenic lines was significantly greater than that of the nontransgenics. Although the degree of chilling and freezing tolerance in the transgenic plants was not directly correlated with the accumulated levels of CbCOR15, we could conclude that this gene confers cold tolerance.

Demethylation of CpG islands in the 5` upstream regions mediates the expression of the human testis-specific gene MAGEB16 and its mouse homolog Mageb16

( Yun Qiang Liu ),( Mei Ling Wang ),( Si Yuan Jiang ),( Yong Jie Lu ),( Da Chang Tao ),( Yuan Yang ),( Yong Xin Ma ),( Si Zhong Zhang ) 생화학분자생물학회 2014 BMB Reports Vol.47 No.2

Tissue-specific gene expression is regulated by epigenetic modification involving trans-acting factors. Here, we identified that the human MAGEB16 gene and its mouse homolog, Mageb16, are only expressed in the testis. To investigate the mechanism governing their expression, the promoter methy-lation status of these genes was examined in different samples. Two CpG islands (CGIs) in the 5` upstream region of MAGEB16 were highly demethylated in human testes, whereas they were methylated in cells without MAGEB16 expression. Similarly, the CGI in Mageb16 was hypomethylated in mouse testes but hypermethylated in other tissues and cells without Mageb16 expression. Additionally, the expression of these genes could be activated by treatment with the demethylation agent 5`-aza-2`- deoxycytidine (5`-aza-CdR). Luciferase assays revealed that both gene promoter activities were inhibited by methylation of the CGI regions. Therefore, we propose that the testis-specific expression of MAGEB16 and Mageb16 is regulated by the methylation status of their promoter regions. [BMB Reports 2014; 47(2): 86-91]

Synthesis and Biological Evaluation of 3,6-diaryl-7H-thiazolo[3,2-b] [1,2,4]triazin-7-one Derivatives as Acetylcholinesterase Inhibitors

Zhe Jin,Liu Yang,Si-Jie Liu,Jian Wang,Shuo Li,Huang-Quan Lin,David Chi Cheong Wan,Chun Hu 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.10

Acetylcholinesterase (AChE) inhibitors played an important role in developing a cure for Alzheimer’ s disease. In order to study on the influence of modifications at different groups and side chains on the AChE inhibitory ability and the active sites of 7H-thiazolo[3,2-b][1,2,4]triazin-7-one derivatives, fourteen 3,6-diaryl-7H-thiazolo[3,2-b][1,2,4]triazin-7-one derivatives were designed and synthesized. The study of AChE inhibitory activity was carried out using the Ellman colorimetric assay with huperzine-A as the positive control drug. Most of the target compounds exhibited more than 50% inhibition at 10 μM. Some target compounds showed strong inhibition against AChE. The molecular fields analysis and preliminary structureactivity relationships are discussed.

일본 애니메이션 〈신세기 에반게리온〉으로 본 메카닉 디자인의 정체성 분석

이종한(Lee, Jong-Han),유사첩(Liu, Si-Jie) 한국만화애니메이션학회 2018 만화애니메이션연구 Vol.- No.50

일본의 메카물 애니메이션은 전 세계 범위에서 널리 알려져 있고 유명한 작품들이 많이 있다. 1952년 , 일본 최초의 메카닉 애니메이션이며 최초의 TV 애니메이션인 〈우주소년아톰〉은 탄생한 후부터 긴 세월에 걸쳐 지금까지 인기가 있다. 아톰은 한 시대의 코드로서 많은 사람에게 영향을 주었다. 일본 메카닉 애니메이션은 자신만의 독특한 특색과 세계관을 대중에게 전달하고 있다. 본 논문은 일본 90년대에서부터 지금까지 폭발적인 인기를 얻고 있는〈신세기 에반게리온〉메카닉 디자인을〈기동전사 건담〉과 비교분석을 통해 일본메카닉 디자인의 변화를 알아보고자 함에 있다. 이런 분석 결과는 애니메이션에 반영된 일본 문화와 사상을 알려 줄 것이며 이는 애니메이션의 문화반영을 보여주는 좋은 사례가 될 것이 기대한다. 〈신세기 에반게리온〉은 1995년에 상영한 후 일본 애니메이션계에 예상치 못한 영향을 주었으며 지금까지도 이를 재해석하고 분석하는 사람들이 끊임없이 나오고 있다. 이는 작품 자체가 가지고 있는 미스테리와 끝나지 않은 결론을 예상하기 위한 관객들의 반응이라 할 수 있다. 그런 특징을 반영하듯 에반게리온의 디자인에도 다른 메카물과 차별되는 디자인적 요소들을 찾아볼 수 있다. 인체 생명공학을 기반으로 한 메카닉 디자인은 기계라는 한계성을 극복하고 더욱 인간다운 모습을 느낄 수 있도록 한다. 인간 본성을 담을 수 있는 파일럿의 탑승구조는 엔터리 플러그라는 형식으로 강화되었고, 전굴 자세는 꼿꼿한 로봇이 아닌 인간다움을 더 부각시킨다. 이렇듯 〈신세기 에반게리온〉은 인간의 정체성을 반영할 수 있는 메카닉 디자인을 추구하고 있다. 80년대의 메카닉 애니메이션으로 〈건담〉을 뽑을 수 있는데, 90년대의 〈신세기 에반게리온〉이 이를 전혀 다른 디자인으로 보여주고 있다. 그러므로 두 작품의 메카닉 디자인을 비교해봄으로써 작품의 메시지와 디자인의 상관관계를 살펴 본다. 〈신세기 에반게리온〉에서 메카닉 디자인의 정체성과 작품 내용의 밀접한 관계를 제시한다. 향후 메카닉 디자인에게 좋은 사례 및 이론적인 바탕이 될 수 있음을 시사하려고 한다. Japan"s mechanic animation is widely known throughout the world. 1952년, Japan"s first mechanic animation and the first TV animation, 〈Mighty Atom〉, has been popular since it"s creation in 1952. Atom, a big hit at the time, has influenced many people. Japanese mechanic animations convey their unique traits and world view to the public In this paper, we are going to discuss the change of the Japanese mechanical design through comparison of the <Neon Genesis Evangelion> mechanical design, which has been booming since the 1990s in Japan; and the 〈Mobile suit Gundam〉. I expect the results of this analysis to depict Japanese culture and thought reflected in animation, which is a good indication of worldwide cultural view of animation. 〈Neon Genesis Evangelion〉 unexpectedly influenced the Japanese animation industry after it screened in 1995, and there are still people constantly reinterpreting and analyzing it. This is the reaction of the audience to anticipate the mystery and endless conclusions of the work itself. The design elements of Evangelion are distinguished from other mechanical objects. Mechanic design based on human biotechnology can overcome limitations of machine and make you feel more human. The pilot "s boarding structure, which can contain human nature, is reinforced in the form of an enterprising plug, and the attitude of excavation makes humanity more prominent than a straight robot. Thus, 〈Neon Genesis Evangelion〉 pursues a mechanic design that can reflect human identity. 〈Gundam〉 can be selected as the mechanic animation of the 80"s, and the "Neon Genesis Evangelion" of the 90"s shows it with a completely different design. By comparing the mechanical design of two works, therefore, we examine the correlation between the message and the design of the work. 〈New Century Evangelion〉 presents the close relationship between the identity of the mechanical design and the contents. I would like to point out that mechanical design can be a good example and theoretical basis for the future.

PKM2 Regulates Hepatocellular Carcinoma Cell Epithelial-mesenchymal Transition and Migration upon EGFR Activation

Fan, Fang-Tian,Shen, Cun-Si,Tao, Li,Tian, Chao,Liu, Zhao-Guo,Zhu, Zhi-Jie,Liu, Yu-Ping,Pei, Chang-Song,Wu, Hong-Yan,Zhang, Lei,Wang, Ai-Yun,Zheng, Shi-Zhong,Huang, Shi-Le,Lu, Yin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Pyruvate kinase isozyme type M2 (PKM2) was first found in hepatocellular carcinoma (HCC), and its expression has been thought to correlate with prognosis. A large number of studies have demonstrated that epithelial-mesenchymal transition (EMT) is a crucial event in hepatocellular carcinoma (HCC) and associated metastasis, resulting in enhanced malignancy of HCC. However, the roles of PKM2 in HCC EMT and metastasis remain largely unknown. The present study aimed to determine the effects of PKM2 in EGF-induced HCC EMT and elucidate the molecular mechanisms in vitro. Our results showed that EGF promoted EMT in HCC cell lines as evidenced by altered morphology, expression of EMT-associated markers, and enhanced invasion capacity. Furthermore, the present study also revealed that nuclear translocation of PKM2, which is regulated by the ERK pathway, regulated ${\beta}$-catenin-TCF/LEF-1 transcriptional activity and associated EMT in HCC cell lines. These discoveries provide evidence of novel roles of PKM2 in the progression of HCC and potential therapeutic target for advanced cases.