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- 저자 : Shoemaker Randy (검색결과 4 건)
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Down - Regulation of Gene Systems in Higher Plants
Sang Gyu Park,Eberson Calvo,Randy Shoemaker,Robert Thornburg 한국응용생명화학회 1992 한국응용생명화학회 학술발표회 Vol.1992 No.-
Ribozymes (hammerhead and hairpin structures) which suppose to down-regulate GUS or CAT expression were constructed with the wound-inducible pin2K and constitutive CaMV 35S promoters. These ribozyme constructions have been successfully tested against their respective target sequences using in vitro transcription reactions. Transgenic plants were selected on MS medium supplemented with hygromycin B. After these plants flowered, crosses will be made to combine the ribozyme constructs with the following transgenes; 1) the wound-inducible CAT gene and the constitutive GUS gene 2) the wound-inducible GUS gene and the constitutive CAT gene 3) the constitutive CAT gene and the constitutive GUS gene. The level of expression of RNA will be correlated with the level of expression of the reporter protein. In addition, another ribozymes which may attack the viral RNAs (CMV and RBSDV RNAs) were synthesized and put in constructions containing CaMV 35S promoter and nos terminator. Sequence analysis are in progress to make sure the constructions. The resistance of transgenic plants against the viral infections will provide us the another way to develop the virus-resistant plants.
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Sequencing crop genomes: approaches and applications
Jackson, Scott A.,Iwata, Aiko,Lee, Suk‐,Ha,Schmutz, Jeremy,Shoemaker, Randy Blackwell Publishing Ltd 2011 The New phytologist Vol.191 No.4
<P><B>Contents</B></P><P> <tabularFixed><table frame='none'><tgroup cols='3' align='left'><colspec colname='col1' colnum='1'/><colspec colname='col2' colnum='2'/><colspec colname='col3' colnum='3'/><tbody valign='top'><row><entry/><entry>Summary</entry><entry>915</entry></row><row><entry>I.</entry><entry>Genomics and crop improvement</entry><entry>915</entry></row><row><entry>II.</entry><entry>Complexity of plant genomes</entry><entry>916</entry></row><row><entry>III.</entry><entry>Evolution of genome sequencing</entry><entry>917</entry></row><row><entry>IV.</entry><entry>Future of genome sequencing</entry><entry>919</entry></row><row><entry>V.</entry><entry>Application of genomics for crop improvement</entry><entry>920</entry></row><row><entry>VI.</entry><entry>Unlocking the potential of genetic diversity through genomic approaches</entry><entry>922</entry></row><row><entry/><entry>Acknowledgements</entry><entry>923</entry></row><row><entry/><entry>References</entry><entry>923</entry></row></tbody></tgroup></table></tabularFixed> </P><P><B>Summary</B></P><P>Many challenges face plant scientists, in particular those working on crop production, such as a projected increase in population, decrease in water and arable land, changes in weather patterns and predictability. Advances in genome sequencing and resequencing can and should play a role in our response to meeting these challenges. However, several barriers prevent rapid and effective deployment of these tools to a wide variety of crops. Because of the complexity of crop genomes, <I>de novo</I> sequencing with next‐generation sequencing technologies is a process fraught with difficulties that then create roadblocks to the utilization of these genome sequences for crop improvement. Collecting rapid and accurate phenotypes in crop plants is a hindrance to integrating genomics with crop improvement, and advances in informatics are needed to put these tools in the hands of the scientists on the ground.</P>
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Van, Kyujung,Kim, Dong Hyun,Cai, Chun Mei,Kim, Moon Young,Shin, Jin Hee,Graham, Michelle A.,Shoemaker, Randy C.,Choi, Beom-Soon,Yang, Tae-Jin,Lee, Suk-Ha Oxford University Press 2008 DNA research Vol.15 No.2
<P>A single recessive gene, <I>rxp</I>, on linkage group (LG) D2 controls bacterial leaf-pustule resistance in soybean. We identified two homoeologous contigs (GmA and GmA′) composed of five bacterial artificial chromosomes (BACs) during the selection of BAC clones around <I>Rxp</I> region. With the recombinant inbred line population from the cross of Pureunkong and Jinpumkong 2, single-nucleotide polymorphism and simple sequence repeat marker genotyping were able to locate GmA′ on LG A1. On the basis of information in the Soybean Breeders Toolbox and our results, parts of LG A1 and LG D2 share duplicated regions. Alignment and annotation revealed that many homoeologous regions contained kinases and proteins related to signal transduction pathway. Interestingly, inserted sequences from GmA and GmA′ had homology with transposase and integrase. Estimation of evolutionary events revealed that speciation of soybean from <I>Medicago</I> and the recent divergence of two soybean homoeologous regions occurred at 60 and 12 million years ago, respectively. Distribution of synonymous substitution patterns, <I>K</I><SUB>s</SUB>, yielded a first secondary peak (mode <I>K</I><SUB>s</SUB> = 0.10–0.15) followed by two smaller bulges were displayed between soybean homologous regions. Thus, diploidized paleopolyploidy of soybean genome was again supported by our study.</P>
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