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RISS 인기검색어
- 검색키워드
- 저자 : Eberson Calvo (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
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Down - regulation of gene systems in higher plants
Park, Sang Gyu,Calvo, Eberson,Shoernaker, Randy,Thornburg, Robert 한국농화학회 1992 Applied Biological Chemistry (Appl Biol Chem) Vol.35 No.5
Ribozymes (hammerhead and hairpin structures) which suppose to down-regulate GUS or CAT expression were constructed with the wound-inducible pin2K and constitutive CaMV 35S promoters. These ribozyme constructions have been successfully tested against their respective target sequences using in vitro transcription reactions. Transgenic plants were selected on MS medium supplemented with hygromycin B. After these plants flowered, crosses will be made to combine the ribozyme constructs with the following transgenes; 1) the wound-inducible CAT gene and the constitutive GUS gene 2) the wound-inducible GUS gene and the constitutive CAT gene 3) the constitutive CAT gene and the constitutive GUS gene. The level of expression of RNA will be correlated with the level of expression of the reporter protein. In addition, another ribozymes which may attack the viral RNAs (CMV and RBSDV RNAs) were synthesized and put in constructions containing CaMV 35S promoter and nos terminator. Sequence analysis are in progress to make sure the constructions. The resistance of transgenic plants against the viral infections will provide us the another way to develop the virus-resistant plants.
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Down - Regulation of Gene Systems in Higher Plants
Sang Gyu Park,Eberson Calvo,Randy Shoemaker,Robert Thornburg 한국응용생명화학회 1992 한국응용생명화학회 학술발표회 Vol.1992 No.-
Ribozymes (hammerhead and hairpin structures) which suppose to down-regulate GUS or CAT expression were constructed with the wound-inducible pin2K and constitutive CaMV 35S promoters. These ribozyme constructions have been successfully tested against their respective target sequences using in vitro transcription reactions. Transgenic plants were selected on MS medium supplemented with hygromycin B. After these plants flowered, crosses will be made to combine the ribozyme constructs with the following transgenes; 1) the wound-inducible CAT gene and the constitutive GUS gene 2) the wound-inducible GUS gene and the constitutive CAT gene 3) the constitutive CAT gene and the constitutive GUS gene. The level of expression of RNA will be correlated with the level of expression of the reporter protein. In addition, another ribozymes which may attack the viral RNAs (CMV and RBSDV RNAs) were synthesized and put in constructions containing CaMV 35S promoter and nos terminator. Sequence analysis are in progress to make sure the constructions. The resistance of transgenic plants against the viral infections will provide us the another way to develop the virus-resistant plants.
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