Phenol Removal Performance and Mechanism Using Catalytic Ozonation with the Catalyst of Cobalt-doped α-MnO2

Jie Zhang,Ben Dong,Ying Han,Xiaocui Zhan,Sijie Ge,Shilong He 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.3

In this paper, Cobalt-doped α-MnO2 (i.e., Co-α-MnO2) were synthesized through hydrothermal method. Phenol was employed as targeted pollutants to investigate the catalytic ozonation performance of Co-α-MnO2. Results showed that Co-α-MnO2 significantly improved the phenol removal increased to 97.47 % after 40 min, which was 16.46 %, 38.92 % higher than that of α-MnO2 catalytic ozonation and single ozonation without catalyst. Additionally, the physicochemical properties of α-MnO2 and Co-α-MnO2 were analyzed using technologies such as XRD, TEM, BET and XPS. Compared to α-MnO2, Co-α-MnO2 has larger specific surface area (79.496 m2/g) and pore volume (0.0396 cm3/g), higher Mn3+ relative content (41.16 %) and adsorbed oxygen content (18.99 %). Also, the oxygen vacancy content, lattice defect content and surface hydroxyl content of Co-α-MnO2 are higher than that of α-MnO2, which could result in higher catalytic oxidation performance of Co-α-MnO2. The influence of masking agent showed that surface hydroxyl group, •OH and •O2− were involved in the catalytic ozonation of phenol. This study could help recognize the role of surface hydroxyl groups and active free radicals and demonstrate the contribution of reactive oxygen species on phenol removal in Co-α-MnO2 systems.

Removal efficiency and mechanism of bio-treated coking wastewater by catalytic ozonation using MnO₂ modified with anionic precursors

Jie Zhang,Ben Dong,Ding Ding,Shilong He,Sijie Ge 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.5

In this paper, MnO₂ catalyst were firstly prepared and modified by four kinds of anionic precursors (i.e., NO₃⁻, AC⁻, SO₄<SUP>2-</SUP> and Cl-) through redox precipitation method. After that, bio-treated coking wastewater (BTCW) was prepared and employed as targeted pollutants to investigate the catalytic ozonation performance of prepared-MnO₂ catalyst was investigated and characterized by the removal efficiencies and mechanism of the prepared bio-treated coking wastewater (BTCW), which was employed as the targeted pollutants. Specifically, the effects of specific surface area, crystal structure, valence state of Mn element and lattice oxygen content on catalytic activity of MnO₂ materials were characterized by BET, XRD and XPS, respectively. Results showed that COD of BTCW could be removed 47.39% under MnO₂-NO₃⁻ catalyst with 2 h reaction time, which was much higher than that of MnO₂-AC⁻ (3.94%), MnO₂-SO₄<SUP>2-</SUP> (12.42%), MnO₂-Cl⁻ (12.94%) and pure O₃ without catalyst (21.51%), respectively. So, MnO₂-NO₃⁻ presented the highest catalytic performance among these catalysts. The reason may be attributed to a series of better physiochemical properties including the smaller average grain, the larger specific surface area and active groups, more crystal defect and oxygen vacancy, higher relative content of Mn<SUP>3+</SUP> and adsorbed oxygen (Oads) than that of another three catalysts.

Compact Phase Detector for Optical-Microwave Synchronization Using Polarization Modulation

Wei, Juan,Zhang, Shuangyou,Kim, Jungwon,Pan, Shilong IEEE 2018 Journal of Lightwave Technology Vol.36 No.19

<P>We propose and demonstrate a compact optical-microwave phase detector taking advantage of polarization modulation. The proposed polarization modulator-based phase detector (PolM-PD) shows 460-attosecond residual timing jitter integrated from 1 Hz to 100 kHz when synchronizing an 8-GHz dielectric resonator oscillator to a free-running 250-MHz mode-locked Er-fiber laser. The absolute single-sideband phase noise of the locked 8-GHz DRO is –138 dBc/Hz (–165 dBc/Hz) at an offset frequency of 10 kHz (10 MHz). The proposed PolM-PD has a compact structure implemented by all commercially available components. Because no fiber loop and delay line are used, the proposed PolM-PD can be potentially integrated on a chip.</P>

Antimicrobial and antioxidant capacity of glucosamine-zinc(II) complex via non-enzymatic browning reaction

Wu, Shuping,Dai, Xiangzi,Shilong, Fangdi,Zhu, Maiyong,Shen, Xiaojuan,Zhang, Kan,Li, Songjun 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.1

Coordination compounds play an important role in the life process, and have been widely used in food, cosmetics and pharmaceutical industry. Herein, we have developed a novel kind of glucosamine-zinc(II) complex (GlcN-ZC) for food additive using non-enzymatic browning reaction. The GlcN-ZC was characterized by FTIR and XRD. Moreover, UV absorbance changes, browning intensity, fluorescence changes, antioxidant activity and antimicrobial assessment of GlcN-ZC were also evaluated. Results showed the GlcN-ZC intermediate compounds were accumulated in non-enzymatic browning while prolonging heating time and melanoidins were produced in the final stage. The fluorescence changes confirmed that fluorophores were formed during the non-enzymatic reaction and fluorescence intensity reached a maximun at 60 min. The highest radical scavenging activity of GlcN-ZC formed after 180 min of heating was 79.2%. Furthermore, GlcN-ZC exhibited excellent antibacterial activity against E. coli and S. aureus. Therefore, GlcN-ZC can be used as a novel promising additive in the food industry.

Enantioselective Synthesis of <i>anti</i>–<i>syn</i>-Trihalides and <i>anti</i>–<i>syn</i>–<i>anti</i>-Tetrahalides via Asymmetric β-Elimination

Tan, Yu,Luo, Shilong,Li, Dongmei,Zhang, Nan,Jia, Shiqi,Liu, Yidong,Qin, Wenling,Song, Choong Eui,Yan, Hailong American Chemical Society 2017 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.139 No.18

<P>Structural motifs containing contiguous halide bearing stereocenters are common in natural products as well as bioactive molecules. A few successful examples have been reported in the area of asymmetric vicinal dihalogenation of alkenes for accessing dihalogenated products; in this report, an alternative generation method of contiguous halide-bearing stereocenters alpha,beta,gamma,delta relative to carbonyl group in excellent enantioselectivity is proposed by utilizing a Song's oligoEG catalyst-catalyzed asymmetric beta-elimination. According to this methodology, a wide range of anti-syn-trihalides and anti-syn-anti-tetrahalides with high levels of enantioselectivity were synthesized. The synthetic utility of the contiguous halide-bearing stereocenters was demonstrated by several transformations. The results of high-resolution mass spectrometry indicated that the favorable interaction between catalyst and one of the enantiomers of racemic contiguously multihalogenated ketone contributed to the original enantioselectivity of dehydrohalogenation. A deuterium kinetic isotope effect experiment revealed that this beta-elimination reaction proceeds by the E2 mechanism. This strategy opens a new pathway for the asymmetric synthesis of contiguous halide bearing stereocenters of great complexity.</P>

MD2 blockade prevents modified LDL-induced retinal injury in diabetes by suppressing NADPH oxidase-4 interaction with Toll-like receptor-4

Chen Huaicheng,Yan Tao,Song Zongming,Ying Shilong,Wu Beibei,Ju Xin,Yang Xi,Qu Jia,Wu Wencan,Zhang Zongduan,Wang Yi 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Modified LDL-induced inflammation and oxidative stress are involved in the pathogenesis of diabetic retinopathy. Recent studies have also shown that modified LDL activates Toll-like receptor 4 (TLR4) to mediate retinal injury. However, the mechanism by which modified LDL activates TLR4 and the potential role of the TLR4 coreceptor myeloid differentiation protein 2 (MD2) are not known. In this study, we inhibited MD2 with the chalcone derivatives L2H17 and L6H21 and showed that MD2 blockade protected retinal Müller cells against highly oxidized glycated-LDL (HOG-LDL)-induced oxidative stress, inflammation, and apoptosis. MD2 inhibition reduced oxidative stress by suppressing NADPH oxidase-4 (NOX4). Importantly, HOG-LDL activated TLR4 and increased the interaction between NOX4 and TLR4. MD2 was required for the activation of these pathways, as inhibiting MD2 prevented the association of NOX4 with TLR4 and reduced NOX4-mediated reactive oxygen species production and TLR4-mediated inflammatory factor production. Furthermore, treatment of diabetic mice with L2H17 significantly reduced LDL extravasation in the retina and prevented retinal dysfunction and apoptosis by suppressing the TLR4/MD2 pathway. Our findings provide evidence that MD2 plays a critical role in mediating modified LDL-induced cell injury in the retina and suggest that targeting MD2 may be a potential therapeutic strategy.

Circ_0000554 is identified as a cancer‑promoting circRNA in colorectal cancer by regulating the miR‑1205/LASP1 axis

Luo Jinlong,Yang Hua,Peng Xuefeng,Zhang Faqiang,Shu Shilong,Lan Ke,Tu Shengjin,Lu Kai,Cha Xiaoying 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.5

Background: Colorectal cancer (CRC) is a prevalent malignant tumor with poor prognosis. Circular RNAs (circRNAs) are key regulators in the progression of CRC. Our study aimed to disclose the role of circ_0000554 in CRC. Methods: The expression of circ_0000554, miR-1205 and LIM and SH3 protein 1 (LASP1) was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation, invasion and migration were monitored using cell counting kit-8 (CCK-8) assay, EdU assay, transwell assay and wound healing assay respectively. The protein levels of C-myc, matrix metallopeptidase 2 (MMP-2) and LASP1 were detected by western blot. Tumor formation assay in nude mice was conducted to explore the role of circ_0000554 in vivo. The association between miR-1205 and circ_0000554 or LASP1 was identified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Results: circ_0000554 was upregulated in CRC tissues and cells, high circ_0000554 expression was significantly linked to shorter overall survival. Downregulation of circ_0000554 restrained cell growth and metastasis while promoted apoptosis in vitro, and suppressed tumorigenesis of CRC in vivo. Furthermore, mechanism study and rescue experiments confirmed miR-1205 could be sponged by circ_0000554 and its inhibitor reversed the inhibitory effect of circ_0000554 silencing on CRC progression. LASP1 was a target gene of miR-1205 and the upregulation of LASP1 overturned miR-1205-induced effects on CRC cells. Circ_0000554 could elevate LASP1 expression via interacting with miR-1205.

Antimicrobial and antioxidant capacity of glucosamine-zinc(II) complex via non-enzymatic browning reaction

Shuping Wu,Xiangzi Dai,Fangdi Shilong,Maiyong Zhu,Xiaojuan Shen,Kan Zhang,Songjun Li 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.1

Coordination compounds play an important role in the life process, and have been widely used in food, cosmetics and pharmaceutical industry. Herein, we have developed a novel kind of glucosamine-zinc(II) complex (GlcN-ZC) for food additive using non-enzymatic browning reaction. The GlcN-ZC was characterized by FTIR and XRD. Moreover, UV absorbance changes, browning intensity, fluorescence changes, antioxidant activity and antimicrobial assessment of GlcN-ZC were also evaluated. Results showed the GlcN-ZC intermediate compounds were accumulated in non-enzymatic browning while prolonging heating time and melanoidins were produced in the final stage. The fluorescence changes confirmed that fluorophores were formed during the non-enzymatic reaction and fluorescence intensity reached a maximun at 60 min. The highest radical scavenging activity of GlcN-ZC formed after 180 min of heating was 79.2%. Furthermore, GlcN-ZC exhibited excellent antibacterial activity against E. coli and S. aureus. Therefore, GlcN-ZC can be used as a novel promising additive in the food industry.

Nonlinear Buckling Analysis of H-Type Honeycombed Composite Column with Rectangular Concrete-Filled Steel Tube Flanges

Jing Ji,Zhichao Xu,Liangqin Jiang,Chaoqing Yuan,Yunfeng Zhang,Lijian Zhou,Shilong Zhang 한국강구조학회 2018 International Journal of Steel Structures Vol.18 No.4

This paper was concerned with the nonlinear analysis on the overall stability of H-type honeycombed composite column with rectangular concrete-fi lled steel tube fl anges (STHCC). The nonlinear analysis was performed using ABAQUS, a commercially available fi nite element (FE) program. Nonlinear buckling analysis was carried out by inducing the fi rst buckling mode shape of the hinged column to the model as the initial imperfection with imperfection amplitude value of L /1000 and importing the simplifi ed constitutive model of steel and nonlinear constitutive model of concrete considering hoop eff ect. Close agreement was shown between the experimental results of 17 concrete-fi lled steel tube (CFST) specimens and 4 I-beams with top fl anges of rectangular concrete-fi lled steel tube (CFSFB) specimens conducted by former researchers and the predicted results, verifying the correctness of the method of FE analysis. Then, the FE models of 30 STHCC columns were established to investigate the infl uences of the concrete strength grade, the nominal slenderness ratio, the hoop coeffi cient and the fl ange width on the nonlinear stability capacity of SHTCC column. It was found that the hoop coeffi cient and the nominal slenderness ratio aff ected the nonlinear stability capacity more signifi cantly. Based on the results of parameter analysis, a formula was proposed to predict the nonlinear stability capacity of STHCC column which laid the foundation of the application of STHCC column in practical engineering.

Exosomes from adipose-derived stem cells overexpressing Nrf2 accelerate cutaneous wound healing by promoting vascularization in a diabetic foot ulcer rat model

Xue-Li Zhou,Xiaoyun Xie,Weishuai Lian,Rongfeng Shi,Shilong Han,Haijun Zhang,Ligong Lu,Maoquan Li 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Diabetic foot ulcers (DFU) increase the risks of infection and amputation in patients with diabetes mellitus (DM). The impaired function and senescence of endothelial progenitor cells (EPCs) and high glucose-induced ROS likely exacerbate DFUs. We assessed EPCs in 60 patients with DM in a hospital or primary care setting. We also evaluated the therapeutic effects of exosomes secreted from adipose-derived stem cells (ADSCs) on stress-mediated senescence of EPCs induced by high glucose. Additionally, the effects of exosomes and Nrf2 overexpression in ADSCs were investigated in vitro and in vivo in a diabetic rat model. We found that ADSCs that secreted exosomes promoted proliferation and angiopoiesis in EPCs in a high glucose environment and that overexpression of Nrf2 increased this protective effect. Wounds in the feet of diabetic rats had a significantly reduced ulcerated area when treated with exosomes from ADSCs overexpressing Nrf2. Increased granulation tissue formation, angiogenesis, and levels of growth factor expression as well as reduced levels of inflammation and oxidative stress-related proteins were detected in wound beds. Our data suggest that exosomes from ADSCs can potentially promote wound healing, particularly when overexpressing Nrf2 and therefore that the transplantation of exosomes may be suitable for clinical application in the treatment of DFUs.