Autologous Bone-marrow Mesenchymal Cell induced Chondrogenesis: Single-stage Arthroscopic Cartilage Repair

Asode Ananthram Shetty,김석중,Vishvas Shetty,David Stelzeneder,Neha Shetty,Praveen Bilagi,이현진 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.3

We describe a single stage arthroscopic procedure for the treatment of articular cartilage defects in theknee. The novel procedure involves microfracture and application of bone marrow aspirate concentrate cells(BMAC) with hyaluronic acid and fibrin gel. The aim of the study was to evaluate the clinical and radiological out-comes at 2 years. A prospective study of 30 patients with symptomatic ICRS grade III/IV chondral defects, rangingfrom 2-9cm2, who were assessed clinically and radiologically. The surgical procedure involved debridement of thelesion, microfracture and application of concentrated BMAC with HA and fibrin gel under CO2 insufflation. Patientsunderwent morphological MRI, quantitative T2*-mapping and d-GEMRIC scan. Clinical assessment used the Lysh-olm, IKDC and KOOS scores. Radiological assessment used the MOCART score. At 2 year follow-up, Lysholmscore was 80.1, as compared to 50.8 pre-operatively (p<0.05). KOOS (symptomatic) was 92.1, as compared to 65.7pre-operatively. IKDC (subjective) was 83, up from 39 preoperatively. The mean T2* relaxation-times for the repairtissue and native cartilage were 29.1 and 29.9 respectively. Average MOCART score for all lesions was 72. Our tech-nique shows encouraging clinical results at 2 year follow-up. Clinical outcome scores show significant benefit. Themorphological MRI shows good cartilage defect filling and the biochemical MRI (T2*-mapping) suggests hyalinelike repair tissue.

Formation and Characterization of Gallium(III) Complexes with Monoamide Derivatives of 1,4,7‐Triazacyclononane‐1,4,7‐triacetic Acid: A Study of the Dependency of Structure on Reaction pH

Shetty, Dinesh,Choi, Soo Young,Jeong, Jae Min,Hoigebazar, Lathika,Lee, Yun‐,Sang,Lee, Dong Soo,Chung, June‐,Key,Lee, Myung Chul,Chung, Young Keun WILEY‐VCH Verlag 2010 European journal of inorganic chemistry Vol.2010 No.34

<P><B>Abstract</B></P><P>Two monoamide derivatives of 1,4,7‐triazacyclononane‐1,4,7‐triacetic acid (NOTA) conjugated with methylamine (<B>4</B>) or benzylamine (<B>5</B>) were synthesized by treating di‐<I>tert</I>‐butyl 1,4,7‐triazacyclononane‐1,4‐diacetate (<B>1</B>) with 2‐chloro‐<I>N</I>‐benzyl‐ or ‐<I>N</I>‐methylacetamide, followed by an acid cleavage reaction. Complexes of <B>4</B> and <B>5</B> chelated to Ga<SUP>3+</SUP> to give Ga‐<B>4</B> and Ga‐<B>5</B>, respectively, in reaction solutions at different pH values (3 and 5). Complexes Ga‐<B>4</B> and Ga‐<B>5</B> were characterized by single‐crystal X‐ray diffraction and multinuclear NMR spectroscopy. In the solid state, these complexes were isostructural, and the coordination spheres of the metal ions exhibited distorted octahedral geometries. In the case of the Ga‐<B>4</B> complex, which was formed at both pH values, the metal ion is coordinated to the amide nitrogen atom of the modified pendent arm of <B>4</B>. However, in the case of Ga‐<B>5</B>, the metal ion is coordinated to a nitrogen or an oxygen atom of the amide linkage when the pH of the reaction soultion was 5 or 3, respectively. No significant difference was found between the <SUP>1</SUP>H NMR spectra of the complexes formed at pH = 3 and 5. However, <SUP>71</SUP>Ga NMR spectra showed a broad resonance signal and a narrow singlet for the complex synthesized at the lower pH, but only a single narrow singlet for the complex prepared at neutral pH. Variable‐temperature <SUP>1</SUP>H NMR spectra showed that complexes Ga‐<B>4</B> and Ga‐<B>5</B> are rigid in solution. The stability of these complexes in the physiological pH range and at high temperature suggests that NOTA can be used as a bifunctional chelating agent to label biomolecules with radioactive gallium by direct conjugation of the complex to target molecules. This finding could open up a wide range of applications for NOTA‐type bifunctional chelating agents by eliminating the multi‐step synthesis routes required to introduce extra linker groups to the labelling agent.</P>

Flexural bond strength behaviour in OPC concrete of NBS beam for various corrosion levels

Shetty, Akshatha,Venkataramanaa, Katta,Babu Narayan, K.S. Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.49 No.1

Corrosion is one of the primary reasons why structures have limited durability. The present investigation is carried out to study the behavior of RC (Reinforced Concrete) structural members subjected to corrosion. Experimental investigations were carried out on National Bureau of Standard (NBS), RC beam specimens made of Ordinary Portland Cement (OPC) concrete. Load versus deflection behaviour was studied for different levels of corrosion i.e., 2.5%, 5%, 7.5% and 10%. It is observed that for every percentage increase in corrosion level, there is about 1.6% decrease in load carrying capacity. Also as the amount of corrosion increases there is a reduction in bond stress.

Scalp Acupuncture on the Immediate and Prolonged Motor Recovery in Spinal Cord Injury: a Case Report

Shetty Geetha B.,H L Nanjeshgowda 사단법인약침학회 2023 Journal of Acupuncture & Meridian Studies Vol.16 No.5

Spinal cord injury (SCI) is one of the main causes of lifelong motor impairment and is associated with important secondary complications. Thus, multifaceted treatments are needed for early functional recovery. Currently, in cases of SCI, surgery, stem cell treatment, medication, and physical therapy are used to repair and restore neuronal activity. Additionally, encouraging results have been reported on the use of acupuncture to modulate neuronal plasticity. Here, we present an SCI case involving a burst fracture at the L3 level, which was treated for 21 days using scalp acupuncture with residential physical therapy. Activation of the motor area was observed after the 1st day of treatment, with the patient completely regaining power and range of motion in the knees, Additionally, over 21 days, the patient exhibited markedly improved motor recovery and functional outcomes, which had not been observed over the previous six months. This report highlights the importance of complementing scalp acupuncture with intensive physical therapy for better motor recovery.

Synthesis, Anthelmintic and Anti-inflammatory Activities of Some Novel Imidazothiazole Sulfides and Sulfones

Shetty, Nitinkumar S.,Khazi, Imtiyaz Ahmed M.,Ahn, Chul-Jin Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.8

A series of new 6-aryl-3-(3,4-dimethoxy-phenyl)-2-phenylsulfanyl-imidazo[2,1-b]-thiazole (5a-c) and 6-aryl-2-benzenesulfonyl-3-(3,4-dimethoxy-phenyl)imidazo[2,1-b]- thiazole (6a-c) have been prepared and characterized by analytical and spectral methods. The title compounds 5a-c and 6a-c were obtained by the reaction of 4-(3,4-dimethoxy-phenyl)-5-phenylsulfanyl-thiazol-2-ylamine (3) and 5-benzenesulfonyl-4-(3,4-dimethoxy -phenyl)thiazol-2-ylamine (4) with various phenacyl bromides in anhydrous ethanol. These newly synthesized compounds (5a-c and 6a-c) were screened for their anthelmintic and anti-inflammatory activities, where they have displayed better activities.

Stable aluminium fluoride chelates with triazacyclononane derivatives proved by X-ray crystallography and ¹⁸F-labeling study

Shetty, Dinesh,Choi, Soo Young,Jeong, Jae Min,Lee, Ji Youn,Hoigebazar, Lathika,Lee, Yun-Sang,Lee, Dong Soo,Chung, June-Key,Lee, Myung Chul,Chung, Young Keun Royal Society of Chemistry 2011 Chemical communications Vol.47 No.34

<P>A single crystal structure of an aluminium-fluoride complex of a model compound (NODA-benzyl) was studied to understand the co-ordination chemistry. Series of ligands with an extra carboxylic acid linker for biomolecule conjugation were studied for improved <SUP>18</SUP>F-labeling applications.</P> <P>Graphic Abstract</P><P>A study on the crystal structure of an aluminium-fluoride complex allowed us to develop NODA ligands for <SUP>18</SUP>F-labeling with an excellent yield. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1cc13151f'> </P>

Can we beat the biotin–avidin pair?: cucurbit[7]uril-based ultrahigh affinity host–guest complexes and their applications

Shetty, Dinesh,Khedkar, Jayshree K.,Park, Kyeng Min,Kim, Kimoon The Royal Society of Chemistry 2015 Chemical Society reviews Vol.44 No.23

<P>The design of synthetic, monovalent host-guest molecular recognition pairs is still challenging and of particular interest to inquire into the limits of the affinity that can be achieved with designed systems. In this regard, cucurbit[7]uril (CB[7]), an important member of the host family cucurbit[n]uril (CB[n], n = 5-8, 10, 14), has attracted much attention because of its ability to form ultra-stable complexes with multiple guests. The strong hydrophobic effect between the host cavity and guests, ion-dipole and dipole-dipole interactions of guests with CB portals helps in cooperative and multiple noncovalent interactions that are essential for realizing such strong complexations. These highly selective, strong yet dynamic interactions can be exploited in many applications including affinity chromatography, biomolecule immobilization, protein isolation, biological catalysis, and sensor technologies. In this review, we summarize the progress in the development of high affinity guests for CB[7], factors affecting the stability of complexes, theoretical insights, and the utility of these high affinity pairs in different challenging applications.</P>

Evaluation of bonding efficiency between facial silicone and acrylic resin using different bonding agents and surface alterations

Shetty, Uttam Sadashiv,Guttal, Satyabodh Shesharaj The Korean Academy of Prosthodonitics 2012 The Journal of Advanced Prosthodontics Vol.4 No.3

PURPOSE. The aim of the study was to evaluate the effect of 3 silicone primers and 3 surface characterization of acrylic resin surface on bond strength between silicone elastomer and acrylic resin. MATERIALS AND METHODS. 96 Cosmesil silicones bonded to heat-curing acrylic resin were fabricated with the dimension of $75{\times}10{\times}3$ mm. The 3 primers used in this study were G611 platinum primer, A-330 Gold platinum primer, and cyanoacrylates resin. Specimens without primer were used as control. The 3 types of surface characterization done were retentive holes with 1.5 mm in diameter and 0.5 mm deep, retentive beads of 0.6 mm diameter and the third type which was plain without any characterization. The specimens were then checked for bond strength by subjecting them to $180^{\circ}$ peel test on a universal testing machine. The obtained results were then subjected to statistical analysis using 2-way ANOVA and Scheff$\acute{e}$ multiple post hoc procedures. The statistical significance was set at 5% level of significance. RESULTS. The maximum bond strength was seen for samples in which A-330G primer was used followed by G611 primer. The control group showed the minimum bond strength. Surface characterization of retentive holes increased the bond strength considerably as compared to retentive beads and samples without any surface characterization. CONCLUSION. Within the limitations of the study, A-330G primer was more compatible with Cosmesil M511 silicone and has better bonding of Cosmesil to acrylic resin. Retentive holes made on acrylic surface increased the bond strength considerably than those without any surface characterization.

$^{68}Ga$-Labeled Radiopharmaceuticals for Positron Emission Tomography

Shetty, Dinesh,Lee, Yun-Sang,Jeong, Jae-Min The Korea Society of Nuclear Medicine 2010 핵의학 분자영상 Vol.44 No.4

[ $^{68}Ga$ ]is a promising emerging radionuclide for positron emission tomography (PET). It is produced using a $^{68}Ga/^{68}Ga$-generator, and thus, would enable the cyclotronindependent distribution of PET. However, new $^{68}Ga$-labeled radiopharmaceuticals that can replace $^{18}F$-labeled agents like [$^18F$]fluorodeoxyglucose (FDG) are needed. Most of the $^{68}Ga$-labeled derivatives currently used are peptide agents, but the developments of other agents, such as amino acid derivatives, nitroimidazole derivatives, and glycosylated human serum albumin, are being actively pursued in many laboratories. Thus, appearance of new $^{68}Ga$-labeled radiopharmaceuticals with high impact are expected in the near future. Here, we present an overview of $^{68}Ga$-labeled agents in terms of their clinical significances and relevances to the management of certain tumors, and pertinent pre-clinical developments.

Analysis of Postoperative Clinical Outcomes in Cervical Myelopathy due to Ossification of Posterior Longitudinal Ligament Involving C2

Shetty Ajoy Prasad,Singh Neerav Anand,Kalanjiyam Guna Pratheep,Meena Jalaj,Rajasekaran Shanmuganathan,Kanna Rishi Mugesh 대한척추외과학회 2023 Asian Spine Journal Vol.17 No.3

Study Design: Retrospective study.Purpose: To investigate the radiological phenotype, patient and surgery-related risk factors influencing postoperative clinical outcome for cervical myelopathy caused by ossification of the posterior longitudinal ligament involving C2 following posterior instrumented laminectomy and fusion.Overview of Literature: Ossified posterior longitudinal ligament (OPLL) is caused by ectopic ossification of the posterior longitudinal ligament. It can cause neurological impairment and severe disability. For multilevel cervical OPLL, studies have shown good neurological recovery following cord decompression via either an anterior or posterior approach. There is, however, a lacunae in the literature regarding the outcomes of patients with OPLL extending to C2 and above (C2 [+]).Methods: We retrospectively studied 61 patients with C2 (+) OPLL who had posterior instrumented laminectomy and fusion at Ganga Hospital, Coimbatore between July 2011 and January 2021, with a minimum follow-up of 2 years. Data on demographics, clinical outcomes, radiology, and post-surgical outcomes were gathered.Results: Among 61 patients, 56 were males and five were females. The OPLL pattern was mixed in 32 cases (52.5%), continuous in 26 cases (42.6%), segmental in two cases (3.3%), and circumscribed in one patient (1.6%). All of our patients showed signs of neurological improvement after a 24-month follow-up. The mean preoperative modified Japanese Orthopaedic Association (mJOA) score was 10.6 (range, 5–11) and the postoperative mJOA score was 15.8 (range, 12–18). The recovery rate was >75% in 27 patients (44.6%), >50% in 32 patients (52.5%), and >25% in two patients (3.3%). The average recovery rate was 71% (range, 33%–100%). The independent risk factor for predicting recovery rate is the preoperative mJOA score.Conclusions: In C2 (+) OPLL, posterior instrumented decompression and fusion provide a relatively safe approach and satisfactory results.