Joint Lattice-Reduction-Aided Precoder Design for Multiuser MIMO Relay System

( Hua Jiang ),( Hao Cheng ),( Lizhen Shen ),( Guoqing Liu ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.7

Lattice reduction (LR) has been used widely in conventional multiple-input multiple-output (MIMO) systems to enhance the performance. However, LR is hard to be applied to the relay systems which are important but more complicated in the wireless communication theory. This paper introduces a new viewpoint for utilizing LR in multiuser MIMO relay systems. The vector precoding (VP) is designed along with zero force (ZF) criterion and minimum mean square error (MMSE) criterion and enhanced by LR algorithm. This implementable precoder design combines nonlinear processing at the base station (BS) and linear processing at the relay. This precoder is capable of avoiding multiuser interference (MUI) at the mobile stations (MSs) and achieving excellent performance. Moreover, it is shown that the amount of feedback information is much less than that of the singular value decomposition (SVD) design. Simulation results show that the proposed scheme using the complex version of the Lenstra--Lenstra--Lovasz (LLL) algorithm significantly improves system performance.

β3GnT8 Regulates Laryngeal Carcinoma Cell Proliferation Via Targeting MMPs/TIMPs and TGF-β1

Hua, Dong,Qin, Fang,Shen, Li,Jiang, Zhi,Zou, Shi-Tao,Xu, Lan,Cheng, Zhi-Hong,Wu, Shi-Liang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Previous evidence showed ${\beta}1$, 3-N-acetylglucosaminyltransferase 8 (${\beta}3GnT8$), which can extend polylactosamine on N-glycans, to be highly expressed in some cancer cell lines and tissues, indicating roles in tumorigenesis. However, so far, the function of ${\beta}3GnT8$ in laryngeal carcinoma has not been characterized. To test any contribution, Hep-2 cells were stably transfected with sense or interference vectors to establish cell lines that overexpressed or were deficient in ${\beta}3GnT8$. Here we showed that cell proliferation was increased in ${\beta}3GnT8$ overexpressed cells but decreased in ${\beta}3GnT8$ knockdown cells using MTT. Furthermore, we demonstrated that change in ${\beta}3GnT8$ expression had significant effects on tumor growth in nude mice.We further provided data suggesting that overexpression of ${\beta}3GnT8$ enhanced the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) at both the mRNA and protein levels, associated with shedding of tissue inhibitors of metalloproteinase TIMP-2. In addition, it caused increased production of transforming growth factor beta 1 (TGF-${\beta}1$), whereas ${\beta}3GnT8$ gene knockdown caused the reverse effect. The results may indicate a novel mechanism by which effects of ${\beta}3GnT8$ in regulating cellular proliferation are mediated, at least in partvia targeting MMPs/TIMPs and TGF-${\beta}1$ in laryngeal carcinoma Hep-2 cells. The finding may lay a foundation for further investigations into the ${\beta}3GnT8$ as a potential target for therapy of laryngeal carcinoma.

A New Triterpenoid from Brucea javanica

Jian Hua Liu,Jiang Jiang Qin,Hui Zi Jin,Xiao Jia Hu,Ming Chen,Yun Heng Shen,Shi Kai Yan,Wei Dong Zhang 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.5

A new triterpenoid, bruceajavanin C (1), together with bruceosides A and B (2 and 3), bruceines D and E (4 and 5), yadanziosides A and G (6 and 7), (20R)-O-(3)-α-L-arabinopyranosylpregn- 5-ene-3β,20-diol (8), and α-D-glucopyranoside, (3β, 20R)-3-hydroxypregn-5-en-20-yl (9) were isolated from the aerial parts of Brucea javanica. The structure of 1 was elucidated on the basis of 2D-NMR spectroscopic analysis. In addition, compounds 1, 3, 4, 5, and 6 exhibited mild inhibitory effect on NO production in LPS-stimulated RAW264.7 cells.

Novel miniature inverted-repeat transposable elements derived from novel CACTA transposons were discovered in the genome of the ant Camponotus floridanus

Xiao-Min Xiong,Shen-Hua Jiang,Guo-Yin Li 한국유전학회 2016 Genes & Genomics Vol.38 No.12

Fourteen novel miniature inverted-repeat transposable element (MITE) families are found in the Florida carpenter ant genome, Camponotus floridanus. They constitute approximately 0.63 % of the entire genome. Analysis of their insertion time showed that most members of these MITEs were inserted into their host genome in less than 8 million years ago. In addition, the association between MITEs and the noncoding regions of genes in C. floridanus is random. Interestingly, an autonomous partner (named CfTEC) responsible for the amplification of these MITEs was also found in C. floridanus. Meanwhile, we present evidence, based on searches of publicly available databases, that this autonomous element was widespread in animals. Moreover, structure and phylogenetic analyses supported that TECs might represent a novel cade of transposons intermediate between the classic CACTA transposon and TRCs. Finally, their transposition mechanism and impact on host genome evolution were also discussed.

Pokemon Inhibits Transforming Growth Factor β-Smad4-Related Cell Proliferation Arrest in Breast Cancer through Specificity Protein 1

Ling Chen,Jing Zhong,Jiang-Hua Liu,Duan-Fang Liao,Ying-Ying Shen,Xiao-Lin Zhong,Xiao Xiao,Wen-Jun Ding,Xiu-Da Peng,Wei Xiong,Xu-Yu Zu 한국유방암학회 2019 Journal of breast cancer Vol.22 No.1

Purpose: Pokemon, also known as ZBTB7A, belongs to the POZ and Krüppel (POK) family of transcription repressors and is implicated in tumor progression as a key proto-oncogene. This present study aimed at determining the mechanism by which Pokemon inhibits transforming growth factor β (TGFβ)-Smad4 pathway-dependent proliferation arrest of breast cancer cells via specificity protein 1 (SP1). Methods: Over-expressing plasmid or small interfering RNA (siRNA) transfection was used to regulate Pokemon levels. The EdU incorporation assay, MTS assay, and clone formation were used to identify the inhibitory effect of Pokemon siRNA on cell proliferation. Quantitative real-time polymerase chain reaction assay confirmed that Pokemon deletion inhibited the expression of proliferation-associated genes. The dual-luciferase reporter assay, electrophoretic mobility shift assay, and co-immunoprecipitation assay were used to analyze binding between Pokemon, Smad4, and SP1. Results: Pokemon deletion induced proliferation arrest of breast cancer cells and inhibited the expression of proliferation-associated genes, especially Smad4. Pokemon bound with SP1 to interdict Smad4 promoter activity. Information on clinical samples was obtained from The Cancer Genome Atlas data, in which the Pokemon mRNA levels showed a negative correlation with Smad4 levels in different subtypes of breast cancer in two independent datasets. Conclusion: We demonstrated that Pokemon binds to SP1 to down-regulate Smad4 expression, thereby promoting proliferation of breast cancer cells. This suggests that Pokemon is a potential TGFβ-signaling participant in breast cancer progression.

Genome-wide identification and evolution of TC1/Mariner in the silkworm (Bombyx mori) genome

Li‑Qin Xie,Ping‑Lan Wang,Shen‑Hua Jiang,Ze Zhang,Hua‑Hao Zhang 한국유전학회 2018 Genes & Genomics Vol.40 No.5

TC1/Mariner transposons belong to class II transposable elements (TEs) that use DNA-mediated “cut and paste” mechanism to transpose, and they have been identified in almost all organisms. Although silkworm (Bombyx mori) has a large amount of TC1/Mariner elements, the genome wide information of this superfamily in the silkworm is unknown. In this study, we have identified 2670 TC1/Mariner (Bmmar) elements in the silkworm genome. All the TEs were classified into 22 families by means of fgclust, a tool of repetitive sequence classification, seven of which was first reported in this study. Phylogenetic and structure analyses based on the catalytic domain (DDxD/E) of transposase sequences indicated that all members of TC1/Mariner were grouped into five subgroups: Mariner, Tc1, maT, DD40D and DD41D/E. Of these five subgroups, maT rather than Mariner possessed most members of TC1/Mariner (51.23%) in the silkworm genome. In particular, phylogenetic analysis and structure analysis revealed that Bmmar15 (DD40D) formed a new basal subgroup of TC1/Mariner element in insects, which was referred to as bmori. Furthermore, we concluded that DD40D appeared to intermediate between mariner and Tc1. Finally, we estimated the insertion time for each copy of TC1/Mariner in the silkworm and found that most of members were dramatically amplified during a period from 0 to 1 mya. Moreover, the detailed functional data analysis showed that Bmmar1, Bmmar6 and Bmmar9 had EST evidence and intact transposases. These implied that TC1/Mariner might have potential transpositional activity. In conclusion, this study provides some new insights into the landscape, origin and evolution of TC1/Mariner in the insect genomes.

Pathological Lesions and Inducible Nitric Oxide Synthase Expressions in the Liver of Mice Experimentally Infected with Clonorchis sinensis

Qing-Li Yang,Ji-Qing Shen,Yan Xue,Xiao-Bing Cheng,Zhi-Hua Jiang,Yi-Chao Yang,Ying-Dan Chen,Xiao-Nong Zhou 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.6

The nitric oxide (NO) formation and intrinsic nitrosation may be involved in the possible mechanisms of liver fluke-associated carcinogenesis. We still do not know much about the responses of inducible NO synthase (iNOS) induced by Clonorchis sinensis infection. This study was conducted to explore the pathological lesions and iNOS expressions in the liver of mice with different infection intensity levels of C. sinensis. Extensive periductal inflammatory cell infiltration, bile duct hyperplasia, and fibrosis were commonly observed during the infection. The different pathological responses in liver tissues strongly correlated with the infection intensity of C. sinensis. Massive acute spotty necrosis occurred in the liver parenchyma after a severe infection. The iNOS activity in liver tissues increased, and iNOS-expressing cells with morphological differences were observed after a moderate or severe infection. The iNOS-expressing cells in liver tissues had multiple origins.

Evaluation of Several Screening Approaches for Detection of Cervical Lesions in Rural Shandong, China

Zong, Li-Ju,Zhang, You-Zhong,Yang, Xing-sheng,Jiang, Jie,Cui, Bao-Xia,Qiao, Yun-Bo,Li, Li,Jiang, Kan,Zhang, Wen-Jing,Kong, Bei-Hua,Shen, Keng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Purpose: The study was designed to: (1) investigate the prevalence of high-risk human papillomavirus (HR-HPV) infection and cervical neoplasia; and (2) evaluate clinical performance of visual inspection with acetic acid/ Lugol's iodine (VIA /VILI), Pap smear, high-risk human papillomavirus (HR-HPV) DNA test for detecting cervical intraepithelial neoplasia grade 2 or worse (CIN2+) and (3) explore appropriate screening approach in rural areas of Shandong Province. Materials and Methods: A total of 3,763 eligible women from Yiyuan County in Yimeng mountainous areas of rural Shandong, China, were enrolled and underwent Pap smear, HR-HPV DNA testing by Hybrid Capture 2 (HC2), and VIA /VILI tests. Women positive in any test were referred to colposcopy and biopsy as indicated. Results: The prevalence of HR-HPV infection among all enrolled women was 11.1% and that in healthy women was 9.9%. In total 33 cases of CIN1, 16 cases of CIN2, 6 cases of CIN3 but none of cervical cancer were detected and the crude prevalence of CIN2+ was 0.58%. For detecting CIN2+, the sensitivity of HR-HPV DNA testing, VIA/VILI, Pap smear was 90.9%, 77.3%, 81.8%, respectively. Pap smear had the best specificity of 98.2%, followed by HR-HPV DNA testing with specificity of 89.4%, VIA/VILI had the lowest specificity of 81.2%. Colposcopy referral rate of HR-HPV DNA testing, VIA/VILI, Pap smear was 11.1%, 18.5%, 2.3%, respectively. Conclusions: Our results suggest that HR-HPV DNA testing alone might be appropriate for primary cervical cancer screening in rural low-resource areas of Shandong Province, China.

Characteristics and Comparisons of Morphometric Measurements and Computed Tomography Hounsfield Unit Values of C2 Laminae for Translaminar Screw Placement Between Patients With and Without Basilar Invagination

Lu-Ping Zhou,Jin Shang,Zhi-Gang Zhang,Zhen-Fei Jiang,Hua-Qing Zhang,Chong-Yu Jia,Ren-Jie Zhang,Cai-Liang Shen 대한척추신경외과학회 2022 Neurospine Vol.19 No.4

Objective: Patients with basilar invagination (BI) had high incidences of vertebral variations and high-riding vertebral artery (HRVA) that might restrict the use of pedicle or pars screw and increase the use of translaminar screw on axis. Here, we conducted a radiographic study to investigate the feasibility of translaminar screws and the bone quality of C2 laminae in patients with BI, which were compared with those without BI as control to provide guidelines for safe placement. Methods: In this study, a total of 410 patients (205 consecutive patients with BI and 205 matched patients without BI) and 820 unilateral laminae of the axis were included at a 1:1 ratio. Comparisons with regard to insertion parameters (laminar length, thickness, angle, and height) for C2 translaminar screw placement and Hounsfield unit (HU) values for the assessment of the appropriate bone mineral density of C2 laminae between BI and control groups were performed. Besides, the subgroup analyses based on the Goel A and B classification of BI, HRVA, atlas occipitalization, and C2/3 assimilation were also carried out. Furthermore, the factors that might affect the insertion parameters and HU values were explored through multiple linear regression analyses. Results: The BI group showed a significantly smaller laminar length, thickness, height, and HU value than the control group, whereas no significant difference was observed regarding the laminar angle. By contrast, the control group showed significantly higher rates of acceptability for unilateral and bilateral translaminar screw fixations than the BI group. Subgroup analyses showed that the classification of Goel A and B, HRVA, atlas occipitalization, and C2/3 assimilation affected the insertion parameters except the HU values. Multiple linear regression indicated that the laminar length was significantly associated with the male gender (B = 0.190, p < 0.001), diagnoses of HRVA (B = -0.109, p < 0.001), Goel A (B = -0.167, p < 0.001), and C2/3 assimilation (B = -0.079, p = 0.029); the laminar thickness was significantly associated with the male gender (B = 0.353, p < 0.001), diagnoses of HRVA (B = -0.430, p < 0.001), Goel B (B = -0.249, p = 0.026), and distance from the top of odontoid to the Chamberlain line (B = -0.025, p = 0.003); laminar HU values were significantly associated with age (B = -2.517, p < 0.001), Goel A (B = -44.205, p < 0.001), Goel B (B = -25.704, p = 0.014), and laminar thickness (B = -11.706, p = 0.001). Conclusion: Patients with BI had narrower and smaller laminae with lower HU values and lower unilateral and bilateral acceptability for translaminar screws than patients without BI. Preoperative 3-dimensional computed tomography (CT) and CT angiography were needed for BI patients.

한국산 참다래 메탄올 추출물의 생리활성 효과

박용서(Yong Seo Park),김병운(Byoung Woon Kim),김태춘(Tae-Choon Kim),장홍기(Hong Gi Jang),천상욱(Sang Uk Chon),조자용(Ja Yong Cho),江??(Shen Hua Jiang),허북구(Buk Gu Heo) 한국원예학회 2008 원예과학기술지 Vol.26 No.4

참다래의 생리활성 물질에 대한 기초자료 확보측면에서 2007년 11월 5일에 참다래 4품종(‘비단’, ‘대흥’, ‘제시골드’ 및 ‘헤이워드’)을 수확하여 2℃에서 30일간 저장한 다음 과피와 과육의 메탄올 추출물에 대한 생리활성 효과를 조사하였다. 총 페놀함량은 추출물이 2,000㎎・L<SUP>-1</SUP>일 때 과피와 과육 모두 ‘대흥’에서 37.6 및 17.3㎎・L<SUP>-1</SUP>로 각각 많았다. 전자공여능은 추출물의 농도가 1,000㎎・L-1일 때 과피는 ‘대흥’에서 89.3%로, 과육은 ‘비단’에서 22.3%로 가장 높았다. 총 플라보노이드 함량은 추출물의 농도가 2,000㎎・L-1일 때 과피는 ‘대흥’에서 17.1㎎・L<SUP>-1</SUP>로, 과육은 비단에서 9.0㎎・L<SUP>-1</SUP>로 가장 높았다. 아질산염 소거는 추출물의 농도가 1,000㎎・L<SUP>-1</SUP>일 때 과피는 ‘대흥’에서 80.9%, 과육은 ‘제시골드’에서 78.3%로 가장 높았다. Tyrosinase 활성 저해 효과는 추출물의 농도가 1,000㎎・L<SUP>-1</SUP>일 때 ‘제시골드’의 과육이 14.4%로 가장 높게 나타났다. 따라서 참다래는 품종 및 부위에 따라 생리활성에 차이가 있었으며, 전반적으로 아질산염 소거능이 높은 것으로 나타났다. This study was conducted to determine the physiological activities of methanol extracts from the fruit skin and flesh of Korean kiwifruit cultivars ‘Bidan’, ‘Daeheung’, ‘Jecygold’ and ‘Hayward’ which were harvested and stored at 2℃ for 30days. At the extract concentration of 2,000 ㎎・L<SUP>-1</SUP>, total phenol content was the highest in fruit skin and flesh of cv. ‘Daeheung’ by 37.6 and 17.3 ㎎・L<SUP>-1</SUP>, respectively. DPPH radical scavenging activity in 1,000 ㎎・L<SUP>-1</SUP> were very high in the fruit skin of kiwifruit cv. ‘Daeheung’ by 89.0% and in the flesh of cv. ‘Bidan’ by 22.3%. Total flavonoid content in the fruit skin of cv. ‘Daeheung’ at 2,000 ㎎・L<SUP>-1</SUP> was 17.1 ㎎・L<SUP>-1</SUP> and in the flesh of cv. ‘Bidan’ 9.0 ㎎・L<SUP>-1</SUP>. Nitrite scavenging activity in 1,000 ㎎・L<SUP>-1</SUP> showed the highest value in the fruit skin of kiwifruit cv. ‘Daeheung’ by 80.9%, and followed by the flesh of cv. ‘Jecygold’ by 78.3%. Mushroom tyrosinase inhibition activity in 1,000 ㎎・L<SUP>-1</SUP> were highest in the flesh of kiwi fruit cv. ‘Jecygold’ by 14.4%. These results indicate that Korean kiwifruits have a high nitrite radical scavenging activity, though the physiological activity of kiwifruits was affected by variety and regional parts.