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        SSR analysis reveals molecular variation and genetic diversity in Malus sieversii with different stress resistance levels in China

        Shaoshuai Yu,Wenxia Zhao,Yanxia Yao,Wenxia Huai,Yuan Cheng,Zhongfu Zhou,Wenfa Xiao,Weiquan Qin,Wei Yan,Weiwei Song 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.6

        Malus sieversii, a valuable crop in Xinjiang, China, is an important natural resource for researching the genetic diversity and phylogenetic evolution of the genus Malus worldwide. Samples from 152 M. sieversii individuals with different symptom grades of deadwood were collected from Gongliu and Xinyuan and analysed with 21 pairs of simple sequence repeat (SSR) primers, revealing molecular genetic characteristics and phylogeny of different groups and individuals. The samples showed high genetic diversity at the species level (Na = 10.76, Ne = 4.37, H = 0.73, I = 1.65, Ho = 0.71, and He = 0.73). Variation was mainly found within groups with lower genetic differentiation (Fst = 0.092) and higher gene flow (Nm = 2.67). A total of 226 alleles were obtained, of which 25 exclusive alleles were from samples with < 40% deadwood rate, and 23 exclusive alleles were from samples with > 40% deadwood rate. Specific bands relating to individuals with deadwood rate < 10% (10 exclusive alleles) or > 60% (7 exclusive alleles) were amplified with 11 pairs of SSR primers. The number of exclusive alleles from M. sieversii plants collected in Gongliu was 52 and from those collected in Xinyuan was 24. Using unweighted pair-group method with arithmetic cluster analysis, the groups with different symptom grades from different sampling sites were shown to be clearly differentiated and formed several discrete subclades. Significantly, the six groups from Gongliu were further classified into two subclusters: Gongliu 1, including three groups with < 40% deadwood rate, and Gongliu 2, including three groups with > 40% deadwood rate.

      • KCI등재

        Temperature dependent luminescence of Dy3þ doped BaYF5 nanoparticles for optical thermometry

        Zhongmin Cao,Shaoshuai Zhou,Guicheng Jiang,Yonghu Chen,Changkui Duan,Min Yin 한국물리학회 2014 Current Applied Physics Vol.14 No.8

        Dy3þ doped BaYF5 nanoparticles with tetragonal structure were synthesized by hydrothermal method and solvothermal method. The structural and the luminescent properties of the samples were characterized by X-ray diffraction pattern, TEM and photoluminescence spectra. Emission of Dy3þ originated from 4I15/2 located at 450 nm and 4F9/2 located at 478 nm, 573 nm and 660 nm were observed under excitation of 355 nm laser. Behavior of fluorescence intensity ratio with temperature increasing from room temperature to approximately 800 K was investigated. And the optimum temperature range for thermometry is obtained to be 550e800 K according to its sensitivity-temperature relation, indicating the potential application of BaYF5:Dy3þ as a luminescent temperature sensor.

      • KCI등재

        Eu3+-site occupation in CaTiO3 perovskite material at low temperature

        Fengfeng Chi,Yanguang Qin,Shaoshuai Zhou,Xiantao Wei,Yonghu Chen,Changkui Duan,Min Yin 한국물리학회 2017 Current Applied Physics Vol.17 No.1

        In order to clarify the site occupancy of rare-earth ions in rare-earth doped perovskite materials, the undoped pure CaTiO3 and Eu3þ-doped CaTiO3 samples with a series of Ca/Ti ratio were synthesized via high-temperature solid-state reaction method. X-ray diffraction (XRD) powder patterns confirm that the crystal structure keeps invariant at various Ca/Ti ratios. Measurement results of unit-cell parameters and X-ray photoelectron spectroscopy (XPS) indicate that Eu3þ ions enter into the Ca2þ site. The highresolution photoluminescence spectra of Eu3þ ions at 20 K in all samples did not witness a significant change under the excitation at different wavelength, implying that Eu3þ ions occupy only one type of site. Considering the small spectral splitting range of 5D0/7F2 transition and the large intensity ratio of 5D0 / 7F2/5D0 / 7F1, it can be concluded that Eu3þ occupies Ca2þ site with larger coordinate numbers rather than Ti4þ site.

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        Advanced Stability Analysis of the Tunnels in Jointed Rock Mass Based on TSP and DEM

        Hongyun Fan,Liping Li,Hongliang Liu,Shaoshuai Shi,Jie Hu,Shen Zhou 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.4

        The discrete element method (DEM) can analyze the large deformation and large displacement of rock mass effectively, and it is widely used in underground engineering, slope engineering and other fields. However, due to the low accuracy of rock mass structural surface information acquisition, the application of discrete element method in the analysis of jointed rock mass stability is still deviated. In this paper, combined with the advantages of the tunnel seismic prediction (TSP) in obtaining discontinuous geological interface information and the discrete element method in the calculation and analysis of jointed rock mass stability, this paper proposes an advanced analysis method for jointed rock mass stability based on TSP and DEM. Compared to the traditional methods, the analysis results of the jointed rock mass stability are more reliable. Firstly, relying on the advanced detection system – Tunnel Seismic Prediction 203Plus, the unstructured rock mass structure information of the tunnel is obtained, and the spatial attitude of the discontinuous geological interface is further determined. Secondly, based on the Fish programming language, the non-continuous geological interface information can be expressed in the discrete unit software – 3D Distinct Element Code (3DEC). In this way, the excavation calculation model of the tunnels in jointed rock mass can be constructed. Finally, based on the DEM, the excavation of the tunnels in jointed rock mass can be simulated, analyze the stability of surrounding rock during the tunnel excavation process, and realize the stability analysis of surrounding rock stability of jointed rock mass. Based on the Huangjiazhuang Tunnel Project, this paper uses the above method to carry out on-site application. The results show that the location of the dangerous block is predicted to be consistent with the actual exposure of the tunnel surrounding rock based on TSP and DEM, which verify the accuracy and feasibility of this method, and the research results have practical guiding significance for the safe construction of the tunnels in jointed rock mass.

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