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Restoration of Lymphokine-activated Killer Cell Response with Indomethacin in Tumor Bearing Mice
Yun, Yeon-Sook,Jung, In-Sung,Yun, Jae-Soon 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-
암에 걸린 생쥐로 부터의 LAK세포의 생성력을 정상 생쥐와 비교하고 암에 걸린 생쥐에서 억제되어 있는 LAK세포 생성력을 PGE₂ synthetase저해제인 indomethacin처리로 회복시키고자 하였다. 암에 걸린 생쥐의 비장세포로 부터의 LAK세포 생성력은 30U/m1이상의 IL-2농도하에서는 정상 생쥐와 같은 수준이였으나 3U/ml의 저농도의 IL-2 존재하에서는 정상 생쥐 보다 낮은 수준이였다. 이와같이 저하되어 있는 LAK세포 생성력은 거식세포에 기인하였다. 거식세포에 의한 LAK세포 생성력 억제는 정상 생쥐에서도 나타났으나 암에 걸린 생쥐의 거tlr세포에 의한 억제작용이 더 크게 나타났다. 암에 걸린 생쥐의 비장세포로 부터 거식세포를 제거하거나 배양액에 indomethacin을 첨가시키므로서 회복되었다. 이상의 실험 결과는 생체내의 PGE₂의 대사조절을 통해 암환자의 면역요법을 호전시킬 수 있는 가능성을 제시하는 것이다.
Yun, Chang-Ho,Jung, Keun-Hwa,Chu, Kon,Kim, So-Hyun,Ji, Ki-Hwan,Park, Hee-Kwon,Kim, Hwan-Cheol,Lee, Soon-Tae,Lee, Sang-Kun,Roh, Jae-Kyu 대한신경과학회 2010 Journal of Clinical Neurology Vol.6 No.2
<P><B>Background and Purpose</B></P><P>Endothelial impairment is a linking mechanism between obstructive sleep apnea (OSA) and cardiovascular diseases. Profiles of endothelial microparticles (EMPs) and endothelial progenitor cells (EPCs) reflect the degree of endothelial impairment. The aims of this study were to measure the levels of EMPs and progenitor cells in OSA, determine the correlations between these factors and OSA severity and the degree of atherosclerosis, and document any changes in these factors after therapy.</P><P><B>Methods</B></P><P>Subjects with (<I>n</I>=82) and without (<I>n</I>=22) OSA were recruited prospectively. We measured the number of colony-forming units (CFU) in cell culture as the endothelial progenitor cell index, and the number of EMPs using flow cytometry with CD31 [platelet endothelial cell adhesion molecule (PECAM)], CD42 (platelet glycoprotein), annexin V, and CD62E (E-selectin) antibodies at baseline and after 4-6 weeks of continuous positive airway pressure (CPAP) therapy. Carotid intima-media thickness (IMT) was regarded as a marker of atherosclerosis.</P><P><B>Results</B></P><P>The levels of PECAM<SUP>+</SUP>CD42<SUP>-</SUP> (<I>p</I><0.001), PECAM<SUP>+</SUP>annexin V<SUP>+</SUP> (<I>p</I><0.001), and E-selectin<SUP>+</SUP> microparticles (<I>p</I>=0.001) were higher in OSA subjects than in non-OSA subjects. The number of CFU did not differ between the two groups. OSA severity independently predicted the levels of PECAM<SUP>+</SUP>CD42<SUP>-</SUP> (<I>p</I>=0.02) and PECAM<SUP>+</SUP>annexin V<SUP>+</SUP> (<I>p</I>=0.004). Carotid IMT was correlated with OSA severity (<I>p</I><0.001), PECAM<SUP>+</SUP>CD42<SUP>-</SUP> (<I>p</I>=0.03), and PECAM<SUP>+</SUP>annexin V<SUP>+</SUP> (<I>p</I>=0.01). Neither OSA severity nor carotid IMT was correlated with either the number of CFU or E-selectin<SUP>+</SUP>. CPAP therapy decreased the occurrence of E-selectin<SUP>+</SUP> (<I>p</I><0.001) in 21 of the OSA subjects, but had no effect on the other microparticles of the number of CFU.</P><P><B>Conclusions</B></P><P>OSA led to the overproduction of EMPs, which moderately correlated with OSA severity and the degree of atherosclerosis, and partly responded to therapy. The endothelial impairment might contribute to future cardiovascular events.</P>
Yun-Hee Sung,Mal-Soon Shin,Sang-Won Lee,Chang-Ju Kim 대한스트레스학회 2009 스트레스硏究 Vol.17 No.4
오미자는 호흡곤란, 기침, 목마름, 기억상실, 그리고 야뇨증 등에 약효를 가지고 있어 치료를 위해 오랫동안 사용된 생약제이다. 천식, 항산화, 간독성, 당뇨 등의 다양한 약효가 보고되고 있지만 항염증과 항진통의 기전에 관한 연구는 미흡한 실정이다. 따라서 본 연구에서는 생쥐 BV2 소교세포에서 lipopolysaccharide (LPS)로 유도한 염증반응 시 오미자추출물의 효과를 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 분석법, 역전사 중합효소 연쇄반응(RT-PCR), Western blot, prostaglandin E2 (PGE2) 면역분석, 그리고 산화질소(NO) 검출법을 이용하여 조사하였다. 오미자 추출물은 LPS로 자극된 BV2 소교세포에서 증가된 cyclooxygenase-2 (COX-2)와 inducible nitric oxide synthase (iNOS)의 발현을 억제시킴으로써 PGE2 합성과 NO 생성을 억제하였다. 또한 오미자 추출물은 인산화된 mitogen- activated protein kinases (MAPKs) 발현도 억제시켰다. 이러한 결과는 오미자가 MAPKs의 하향조절을 통해 COX-2와 iNOS 발현을 억제시켜 항염증과 항진통효과를 나타낼 수 있음을 나타낸다. Schizandrae fructus is a medicinal fruit that has long been used for the treatment of dyspnea, cough, mouth dryness, amnesia, and nocturnal emission. Several studies have reported the anti-asthmatic, anti-hepatotoxic, anti-oxidative, and anti-diabetic effects of Schizandrae fructus; however little is known about the mechanisms by which the anti-inflammatory of this herb. In this study, we evaluated the effects of aqueous extract of Schizandrae fructus on lipopolysaccharide (LPS)-induced inflammation in mouse BV2 microglial cells. To accomplish this, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot, prostaglandin E2 (PGE2) immunoassay, and nitric oxide (NO) detection were conducted. The results revealed that the aqueous extract of Schizandrae fructus suppressed PGE2 synthesis and NO production by inhibiting the LPS-stimulated enhancement of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression in mouse BV2 microglial cells. This extract also suppressed the expression of phosphorylated forms of mitogen-activated protein kinases (MAPKs). These results demonstrate that Schizandrae fructus may exert anti-inflammatory, and that these effects are associated with the suppression of COX-2 and iNOS expressions via down-regulation of MAPKs. (Korean J Str Res 2009;17:415∼424)
Sung, Dong Kyung,Sung, Se In,Ahn, So Yoon,Chang, Yun Sil,Park, Won Soon MDPI AG 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.12
<P>We investigated the role of protease-activated receptor (PAR)-mediated signaling pathways in the biogenesis of human umbilical cord blood-derived mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) and the enrichment of their cargo content after thrombin preconditioning. Immunoblot analyses showed that MSCs expressed two PAR subtypes: PAR-1 and PAR-3. Thrombin preconditioning significantly accelerated MSC-derived EV biogenesis more than five-fold and enriched their cargo contents by more than two-fold via activation of Rab5, early endosomal antigen (EEA)-1, and the extracellular signal regulated kinase (ERK)1/2 and AKT signaling pathways. Blockage of PAR-1 with the PAR-1-specific antagonist, SCH79797, significantly suppressed the activation of Rab5, EEA-1, and the ERK1/2 and AKT pathways and subsequently increased EV production and enriched EV cargo contents. Combined blockage of PAR-1 and PAR-3 further and significantly inhibited the activation of Rab5, EEA-1, and the ERK1/2 and AKT pathways, accelerated EV production, and enriched EV cargo contents. In summary, thrombin preconditioning boosted the biogenesis of MSC-derived EVs and enriched their cargo contents largely via PAR-1-mediated pathways and partly via PAR-1-independent, PAR-3-mediated activation of Rab5, EEA-1, and the ERK1/2 and AKT signaling pathways.</P>
Yun, Hui-Jun,Chung, Dae Sung,Kang, Il,Park, Jong Won,Kim, Yun-Hi,Kwon, Soon-Ki The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.47
<P>A series of triethylsilylethynyl anthracene (TESAN) derivatives, end-capped with bithiophene and thienothiophene, were synthesized by Suzuki coupling. The thermal, optical, electrochemical, and crystalline properties of the TESAN derivatives were reported and correlated with charge transport behavior measured <I>via</I> both a thin film transistor and a single crystal transistor. The TESAN derivative substituted with bithiophene exhibited more efficient π electron delocalization over the molecule compared to the derivative substituted with fused thienothiophene, and consequently gave the highest FET mobility, 1.28 cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP>, from single crystal transistors with negligible hysteresis and almost zero turn-on voltage. The discrepancy of charge carrier mobility between the thin film transistor and single crystal transistor and the solvent annealing effects are discussed in conjunction with morphological and structural analyses.</P> <P>Graphic Abstract</P><P>A series of triethylsilylethynyl anthracenes, end-capped with bithiophene and thienothiophene, are synthesized and used as semiconductors for high mobility transistors. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm34160c'> </P>