Proliferation Effect of Conditioned Medium Produced by Lymph Node Stromal Cells

Lee, Sang-Han,Moon, Sung-Hoon,Ha, Sang-Chul,Ma, Jin-Yeul,Kim, Min-Sook,Lee, Jin-Man,Kwon, O-Yu Korean Society of Life Science 2002 Journal of Life Science Vol.12 No.1

CS21 lymphoma cells that preferentially metastasize to lymph nodes after s.c. inoculation into BALB/c mice were grown in vitro in the presence of CA12 stromal cells isolated from lymph nodes. To obtain fundamental data on identification and characterization of the soluble factor(s) produced by CA12 stromal cells, we investigated the biological profile of the conditioned medium produced by CAl2 stromal cells. CAl2 conditioned medium has no affinity with Con A. CAl2 conditioned medium is associated with the proliferation of splenic T- and thymic T-cells with-out adding mitogen, although the conditioned medium cannot induce the differentiation of thymocytes. Additionally, we showed that H-7, not HA-1004 inhibits CS21 cell proliferation. These results suggest that CAl2 conditioned medium has a specific soluble factor(s) produced by lymph node stromal cells.

몬순기후와 복잡지형의 특성을 갖는 광릉 산림유역의 물과 탄소순환에 대한 교차규모 연구로부터의 교훈

이동호 ( Dong Ho Lee ),김준 ( Joon Kim ),김수진 ( Su Jin Kim ),문상기 ( Sang Ki Moon ),이재석 ( Jae Seok Lee ),임종환 ( Jong Hwan Lim ),손요환 ( Yo Whan Son ),강신규 ( Sin Kyu Kang ),김상현 ( Sang Hyun Kim ),김경하 ( Kyong Ha Kim 한국농림기상학회 2007 한국농림기상학회지 Vol.9 No.2

광릉 슈퍼사이트에서 대기와 식생간의 에너지와 물질교환 규명은 지형과 식생의 복잡성으로 인해 많은 기술적인 어려움에 직면하고 있다. 따라서 KoFlux 연구에서는 상호보완적인 다중 분야의 연구를 통해 이러한 어려움을 극복하기 위한 시도를 해 오고 있다. 본 논문에서는 현재 진행중인 KoFlux 연구의 예비결과 중 특히 광릉사이트에서의 물/탄소수지, 물과 탄소순환의 상호관련성 그리고 몬순기후하의 생태계에서 탄소순환에 미치는 수문학적 요인의 중요성에 대해 중점적으로 논의하고자 한다. 광릉낙엽활엽수림의 순생태생산량 (NEP)은 광범위한 생태학적 연구결과에 따르면 약 ~2.6 t C/ha/y로 예상된다. 이를 미기상 플럭스 관측 결과와 함께 고려할 때 광릉 산림은 탄소의 중요한 육상흡원으로서 기능함을 예상할 수 있다. 다양한 생태수문 관측에 의해 규명된 유역단위 물수지에 의하면 연단위 전체강수량의 약 30-40%가 증발산 (ET)에 해당함을 나타내고 있다. 광릉산림의 대표적인 수종인 졸참과 서어나무의 잎에서 얻어진 탄소 안정동위 원소 조성에 근거하여 계산된 식물 성장기간의 평균 물 이용효율(WUE)은 약 ~12 μmol CO2/mmol H2O에 해당된다. 얻어진 증발산량과 물이용효율은 유역단위의 생태계 생산량을 산출하는데 이용될 수 있다. 나이테 성장량과 토양호흡량의 연단위 변화는 강수량과 강수의 패턴에 의해 크게 영향을 받음을 보이고, 이는 몬순기후에 영향을 받는 동아시아 지역 생태계에서 탄소 순환과정이 수문학적 조건과 밀접한 상관관계를 가짐을 지시한다. 연구지역의 공간적 특성을 정량화하기 위해 관측지의 구조 및 기능적 단위를 규명하기 위한 연구가 진행되고 있고 이를 통해 서로 다른 시공간 규모에서 진행되고 있는 연구의 결과물을 체계적으로 연결 통합하고 나아가 보다 광역적인 규모에서 대표적인 물/탄소 수지를 산출하기 위한 연구가 시도되고 있다. KoFlux Gwangneung Supersite comprises complex topography and diverse vegetation types (and structures), which necessitate complementary multi-disciplinary measurements to understand energy and matter exchange. Here, we report the results of this ongoing research with special focuses on carbon/water budgets in Gwangneung forest, implications of inter-dependency between water and carbon cycles, and the importance of hydrology in carbon cycling under monsoon climate. Comprehensive biometric and chamber measurements indicated the mean annual net ecosystem productivity (NEP) of this forest to be ~2.6 t C ha-1 y-1. In conjunction with the tower flux measurement, the preliminary carbon budget suggests the Gwangneung forest to be an important sink for atmospheric CO2. The catchment scale water budget indicated that 30~40% of annual precipitation was apportioned to evapotranspiration (ET). The growing season average of the water use efficiency (WUE), determined from leaf carbon isotope ratios of representative tree species, was about 12 μmol CO2/mmol H2O with noticeable seasonal variations. Such information on ET and WUE can be used to constrain the catchment scale carbon uptake. Inter-annual variations in tree ring growth and soil respiration rates correlated with the magnitude and the pattern of precipitation during the growing season, which requires further investigation of the effect of a monsoon climate on the catchment carbon cycle. Additionally, we examine whether structural and functional units exist in this catchment by characterizing the spatial heterogeneity of the study site, which will provide the linkage between different spatial and temporal scale measurements.

분포형 토양침식지도를 이용한 한강상류지역 토양유실 위험성 평가

박찬원(Chan-Won Park),손연규(Yeon-Kyu Sonn),장용선(Yong-Seon Zhang),홍석영(S. Young Hong),현병근(Byung-Keun Hyun),송관철(Kwan-Cheol Song),하상건(Sang-Keun Ha),문용희(Young-Hee Moon) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.6

본 연구에서는 1:25,000 정밀토양도 등 가용한 공간자료를 이용하여 작성된 토양유실도를 이용하여 한강 상류지역 10개 중권역 108 소유역에 대하여 토양유실량 평가하고 토지이용별 토양유실 위험지역을 분석하였다. 대상유역 총 토양유실량은 895만 Mg yr<SUP>-1</SUP>으로 예측되었으며, 면적당 평균 토양유실량은 6.1 Mg ha<SUP>-1</SUP>yr<SUP>-1</SUP>이었다. 중소유역 단위로 살펴보면 남한강지역이 북한강지역의 유실량 뿐만 아니라 면적당 유실량이 더 많았다. 이는 북한강 권역의 경우 화강암 및 화강편마암이 주요 모재인 반면에 남한강 권역의 경우 토양모재가 퇴적암지대를 많이 포함있어 토양침식성 인자 (K factor)와 경사 인자 (LS factor)가 상대적으로 높고, 남한강 지역의 경우 농경지 토양이 상대적으로 많이 분포하고 있기 때문으로 판단된다. 대상유역의 토지이용별 연평균 토양유실량을 분석한 결과 산림/초지 > 밭 >> 도심/대지 >> 논 > 과수의 순로 분석 대상유역 중 10.7%를 차지하고 있는 농경지에서 유실되는 토양의 추정량은 41.3%이였으며, 이중 6.2%면적을 차지하는 밭이 40.6%으로 44.2%의 산림/초지와 비슷한 수준이었다. 한강상류지역 토양유실 위험성 평가시 분포형 토양침식지도와 항공사진을 비교한 결과 토양유실에 대한 공간인 정보를 확실하게 보여주고 있으며, 토양침식위험성은 남한강권역의 남한강 상류 (1001), 평창강 (1002), 충주댐 (1003) 권역에서 각각 토양침식위험성이 “보통”이상으로 높은 지역이 각각 8.7%, 7.9%, 7.8%로 평균인 5.9%보다 높았으며, 이에 대한 보전방안이 강구될 필요가 있고 판단된다. This study was conducted to evaluate soil erosion risk with a standard unit watershed in the upper Han river basin using the spatial soil erosion map according to the change of landuse. The study area is 14,577 ㎢, which consists of 10 subbasins, 107 standard unit watersheds. Total annual soil loss and soil loss per area estimated were 895 × 10<SUP>4</SUP> Mg yr<SUP>-1</SUP> and 6.1 Mg ha<SUP>-1</SUP> yr<SUP>-1</SUP>, respectively. A result of analysis with a subbasin as a unit showed that annual soil losses and soil loss per area in Namhan river basins was more than in Bukhan river ones. Predicted annual soil loss according to the landuse ranked as Forest & Grassland > Upland >> Urban & Fallow area > Paddy field > Orchard. Upland area covered 6.2% of the study area, but the contribution of total annul soil loss was 40.6% and that of Forest & Grassland was 44.2%. As a evaluation of soil erosion risk using the spatial soil erosion map, we could precisely conformed the potential hazardous region of soil erosion in each unit watersheds. The ratio of regions, graded as higher “Moderate” for annual soil loss, were respectively 8.7%, 7.9% and 7.8% in 1001, 1002 and 1003 subbasins in Namhan river basin. Most landuse of these area was upland, and these area is necessary to establish soil conservation practices to reduce soil erosion based on the field observation.

논문 : 청원-충주지역 수막재배용 지하수 사용량 및 배출량 분석

문상호 ( Sang Ho Moon ),하규철 ( Kyoo Chul Ha ),김용철 ( Yong Cheol Kim ),윤필선 ( Pil Sun Yoon ) 대한지질공학회 2012 지질공학 Vol.22 No.4

국내 수막재배지는 시설 증대로 인한 지하수 취수량 감소의 어려움을 겪고 있다. 이 연구는 이들 지역 중 청원-충주지역을 대상으로 지하수 이용량 및 배출량을 측정하고, 이를 토대로 국내 수막재배지에서의 지하수 이용량을 추정하였다. 대표 관정들의 모터사양과 1일 지하수 토출 능력과의 관계, 1일 최저 기온0℃ 이하의 기록 일수, 최저 기온 변화에 따른 모터 가동 상황 등을 토대로 하여, 청원지역에서 2011~2012년 겨울철 수막재배에 이용된 지하수 이용량을 추정하면 1ha 당 53,138m3이 된다. 이에 의한 면적 대비 국내 수막재배지 (10,746ha)의 지하수 이용 총량은 약 5.7억 m3이며, 이는 국내 농업용수 지하수 이용량 16.9억 m3 33.7%에 해당된다. 2012년 2월 9일부터 22일까지 면적 4ha의 청원지역 배수로에서 측정한 1일 지하수 배출량은 2,079~2,628 m3범위(평균 2,341 m3)로서, 수막재배 면적 1ha 당 1일 평균 지하수 배출량이 585m3인 것으로 나타났다. 수막재배 일수 94일을 적용하면, 청원지역에서 2011년~2012년 겨울철에 수막재배에 이용된 지하수 이용량은 54,990m3/ha이다. 이에 의한 면적 대비 국내 수막재배지의 지하수 이용 총량은 약 5.9억 m3이며, 이는 전체 농업용수 지하수 이용량의 약 34.9%에 해당된다. 충주지역에서는 수막재배지 1ha 당 1일 지하수 배출량이 805 m3 미만일 것으로 추정되었다. 이 지역에서의 2011년~2012년 겨울철 수막재배 일수 108일을 적용하면, 면적 대비 국내 수막재배지의 지하수 이용량은 전체 농업용수 지하수 이용량의 55% 미만일 것으로 추정된다. Korean agricultural areas that employ water curtain cultivation (WCC) have recently suffered extensive groundwater short-ages due to an increase in the number of facilities. The primary focus of this study is to measure the daily groundwater use and discharge rates in the Cheongweon and Chungju pilot areas, while the second focus is to estimate the total amount of groundwater used in WCC areas nationwide in Korea. Taking inwo consideration several factors, including motor type, out-flow abilities of wells, records of daily minimum temperatures below 0℃, and the number of running wells accrding to weather variations, we estimated that 53,138m3/ha of groundwater had been used in the 4-hectare Cheongweon pilt area during the winter period of late 2011 through early 2012. On a prorated areal basis, we can calculate that the total ground-water used nationwide was 0.57 billion m3 in WCC areas of 10,746m2. This value is equivalent to 33.7% of the total agri-cultural groundwater use (1.69 billion m3) in Korea. During 9-22 February 2012, the daily water discharge rate in the 4-ha Cheongweon pilot area raged from 2,079 to 2,628m3, averaging 2,341m3. Combining this value with meteorological records for 94 days with a daily minimum temperature below 0℃ results in an estimated groundwater volume of 54,990m3/ha for the pilot area during the 2011-2012 winter period. The total amount of groundwater used nationwide in WCC areas would then be 0.59 billion m3, equivalent to 34.9% of the total agricultural groundwater use in Korea. In the Chungju area, the groundwater discharge rate was estimated to be less than 805m3/ha. This value, combined with weather data for 108 days with a daily minimum temperature below 0℃ in this area, can be applied to infer that the total groundwater vol-ume used in WCC areas nationwide is no more than 55% of the total agricultural groundwater use in Korea.

<i>GSTM1</i> Tissue Genotype as a Recurrence Predictor in Non-muscle Invasive Bladder Cancer

Ha, Yun-Sok,Yan, Chunri,Jeong, Pildu,Kim, Won Tae,Yun, Seok-Joong,Kim, Isaac Yi,Moon, Sung-Kwon,Kim, Wun-Jae The Korean Academy of Medical Sciences 2011 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.26 No.2

<P>Tissue genotyping is more useful approach than using blood genomic DNA, which can reflect the effects of the somatic mutations in cancer. Although polymorphisms in glutathione S-transferase (<I>GST</I>) have been associated with the risk of bladder cancer (BC) development, few reports provide information about the prognosis of BC. We investigated glutathione S-transferase mu (<I>GSTM1</I>) and glutathione S-transferase theta (<I>GSTT1</I>) genotypes using genomic DNA from primary 165 BC tissue samples to assess the association with disease prognosis. DNA samples from tumor were analyzed by multiplex polymerase chain reaction (PCR). The results were compared with clinicopathological parameters. The prognostic significance of the <I>GST</I>s was evaluated by Kaplan-Meier and multivariate Cox regression model. Kaplan-Meier estimates revealed significant differences in time to tumor recurrence according to the <I>GSTM1</I> tissue genotype (<I>P</I> = 0.038) in non-muscle invasive bladder cancer (NMIBC). Multivariate Cox regression analysis also revealed that the tissue <I>GSTM1</I> genotype (hazards ratio [HR]: 0.377, <I>P</I> = 0.031) was an independent predictor of bladder tumor recurrence in NMIBC. This identification of <I>GSTM1</I> tissue genotype as a prognosticator for determining recurrence in NMIBC should prove highly useful in a clinical setting.</P>

The Effects of Market Opening on the Korean Retail Industry

Sang Young Moon,Bong Chan Ha 한국경제학회 2009 The Korean Economic Review Vol.25 No.2

Study on the Removal of Cadmium from Underground Water Using a Complex Modulation Electromagnetic Device Based on Correlation of Electromagnetic Field and Electrical Conductivity

Ha, Sang An,Jung, Byong Ho,Lee, Jong Moon,Kim, Kwon Hoo,Wang, Jei Pil Trans Tech Publications, Ltd. 2013 Advanced materials research Vol.813 No.-

<P>The objective of this study was to increase flux and decomposition speed through the development of a multi-type electrical field decomposition facility that employs a more complex modulated electromagnetic field than that used in existing decomposition facilities where recalcitrant organics or heavy metals are combined together. Further, in this study, optimized foundational data was derived from the results obtained following field work. As a result, when an electrical field was applied to an electrolyzer, decomposition of the electrolyzer was carried out quickly, thereby showing higher efficiency because an electrical field was permeated to the contaminant faster compared to the case where an electrolyzer was not added. Keywords: Electromagnetic field, Heavy metal, Electrolyzer, Electrical field</P>

Acacetin Attenuates Neuroinflammation via Regulation the Response to LPS Stimuli In Vitro and In Vivo

Ha, Sang Keun,Moon, Eunjung,Lee, Pyeongjae,Ryu, Jong Hoon,Oh, Myung Sook,Kim, Sun Yeou Springer-Verlag 2012 Neurochem Res Vol.37 No.7

Overexpression of matriptase correlates with poor prognosis in esophageal squamous cell carcinoma

Ha, Sang Yun,Kim, Ki Yeon,Lee, Nam Kyung,Kim, Moon Gyo,Kim, Seok-Hyung Springer-Verlag 2014 Virchows Archiv Vol.464 No.1

A Fundamental Study on the Processing of Heavy Metals Contained in Underground Water by Composite Modulation Electromagnetic Field Processing

Ha, Sang An,Kim, Dong Kyun,Lee, Dong Won,Jung, Byong Ho,Lee, Jong Moon,Wang, Jei Pil Trans Tech Publications 2014 Advanced materials research Vol.1025 No.-

<P>The present study is aimed at deriving study factors appropriate for removal of heavy metals in a region with combined contamination of non-biodegradable organics or heavy metal ions in soil or underground water contamination or a region with underground water contamination using a composite alternating current electromagnetic field with combined modulation of an electric and a magnetic field. In addition, it is directed at deriving fundamental experiment, design factors that can be utilized in removing organic contaminants or metal-organic complexes having polarity of an electric charge. The continuous-type apparatus for fusion process in a composite alternating current electric field employed in the present study was designed to process a maximum of 260 L. Each configuration is provided with electrolytic decomposition apparatus, Magnetic Equipment, Power supply and Electrode plates with an adjustable distance between electrodes. By measuring oxidation and reduction processes of ions appearing in chemical reaction processes, electric potentials produced by injection of a negative reactivity electrode into an aqueous solution in a reversible redox equilibrium condition were measured.</P>