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볏짚 위주 사양 시 비육 흑염소의 농후사료 급여 수준이 발육, 도체특성 및 경제성에 미치는 영향
김상우(Sang Woo Kim),윤세형(Sei Hyung Yoon),김재환(Jae-Hwan Kim),고응규(Yeoung-Gyu Ko),김동훈(Dong hoon Kim),강근호(Geun Ho Kang),김영신(Young-Sin Kim),이상무(Sang Moo Lee),서상원(Sang Won Suh) 한국초지조사료학회 2012 한국초지조사료학회지 Vol.32 No.4
This study was conducted to examine the effect of feeding levels of concentrate feed on growth performance, meat quality and economic evaluation of Korean black goats. The 40 male Korean native goat were divided into four treatment groups T1 (1.5%), T2 (2.0%), T3 (2.5% feeding of concentrate feed body weight per day) and T4 (ab libitum) with ab libitum rice straw. Rice straw intake decrease as against feeding levels, however average daily gains were 22, 50, 69 and 94 g/day in T1, T2, T3 and T4 groups respectively levels (p<0.05). Dressing percentage were 41.77, 42.78, 46.12 and 49.78% in each group (p<0.05), also fat percentage were a significant increase according to feeding levels. In economic efficacy, T4 was higher than other treatment groups. In conclusion, both rice straw and concentrate feed ab libitum are good for feeding and management system on Korean black goats.
Mi-Ran Lee,Sang-Hoon Park,Tae-Suk Kim,Sang-Ki Baek,Sang-Jin Jin,Yeoung-Gyu Ko,Hwan-Hwoo Sung,Ho-Baek Yoon,Jin-Wook Kim,Joon-Hee Lee 경상대학교 농업생명과학연구원 2014 농업생명과학연구 Vol.48 No.6
The cloning efficiency is extremely low despite successful somatic cell nuclear transfer (SCNT) method producing cloned animals in several mammals. In general, faulty epigenetic modifications underlying the incomplete reprogramming of donor cell nuclei after SCNT mainly results in low cloning efficiency. The nuclear reprogramming process involves epigenetic modifications, such as DNA demethylation and histone acetylation, which may be an important factor in enhancing the cloning efficiency. Recently, the histone deacetylase inhibitors (HDACi), such as trichosatin A (TSA) and m-carboxycinnamic acid bishydroxamide (CBHA), to increase histone acetylation have been used to improve the developmental competence of SCNT embryos. Therefore, we compared the effects of TSA with CBHA on the in vitro developmental competence and pluripotency-related gene expression (Oct4, Nanog and Sox2) in porcine cloned blastocysts and histone acetylation pattern (H3K9ac). The porcine cloned embryos were treated with a 50nM concentration of TSA and 100μM concentration of CBHA during the in vitro early culture (10h) after cell fusion and then were assessed to cleavage rate, development to the blastocyst stage and pluripotency-related gene expression in NT blastocyst also, level of histone acetylation in zygote, 2cell, 4cell stage. As results, Although NT, TSA and CBHA treated NT embryos were not different between all groups for cleavage rates, the developmental competence to the blastocyst stage was significantly increased in CBHA treated embryos (22.7%) compared to that of normal NT and TSA treated NT embryos (8.1% and 15.4%)(p<0.05). In addition, all of pluripotent transcription factors (Oct4, Nanog and Sox2) were expressed in the CBHA treated NT embryos, however, Sox2 and Oct4 were expressed in TSA treated NT embryos and expression pattern of CBHA treated NT embryos is particularly similar to that of IVF embryos. Also, CBHA treated NT embryos were increased in level of histone acetylation (H3K9ac) at the zygote, 2-cell, 4-cell stage compared to those of NT and TSA treated NT embryos. In conclusion, the treatment of CBHA as a histone deacetylase inhibitor significantly increased the developmental competence of porcine NT embryos and pluripotency-related gene expressions(Oct4, Nanog and Sox2) in NT blastocysts and level of histone acetylation (H3K9ac).
Dong-Hyouk Jang,Ha-Jung Sung,Won Jun Han,Yang Joong Kim,Sang Yeoung Yoon,Sang Yong Kim,Jae-Hwang Jeong,Jin-Joo Hue,Sang Yoon Nam,Young Won Yun,Beom Jun Lee,Jong-Soo Kim 한국실험동물학회 2009 Laboratory Animal Research Vol.25 No.3
This study was carried out to assess the potential of intranasal administration of ondansetron (a 5-HT₃ receptor antagonist, which antagonize the acute emetic response) in preventing cisplatin-induced emesis in the ferret. After intranasal administration of ondansetron at doses of 0.3 and 0.6 ㎎/㎏, the peak plasma concentrations (Cmax) were 38 and 66.7 ng/mL, and attained at 13 and 23 min (Tmax) respectively. The intravenous (0.3 ㎎/㎏) and intranasal (0.6 ㎎/㎏) administrations of ondansetron significantly delayed the latency time to the first retching and vomiting. In acute phase of emesis during 24 hr, the number of vomits and retches were significantly reduced both in intravenous and intranasal administration groups. In delayed emesis (24-72 hr), intranasal administration of ondansetron at a higher dose of 0.6 ㎎/㎏ or intravenous injection of 0.3 ㎎/㎏ significantly reduced retching but not vomiting. Intranasal ondansetron, administered as 0.6 ㎎/㎏ dose, provided potency in antiemetic control comparable to intravenous ondansetron (0.3 ㎎/㎏) in cisplatin-induced acute emesis, without well known side effects when injected via intravenously. These results suggest that intranasal administration of ondansetron can be an effective substitute for intravenous administration for the prevention of cisplatin-induced acute emesis.
( Yoon E Choi ),( Yeoung Sang Yun ),( Jong Moon Park ),( Ji Won Yang ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.10
An internally radiating photobioreactor was applied for the production of astaxanthin using the unicellular green alga Haematococcus pluvialis. The cellular morphology of H. pluvialis was significantly affected by the intensity of irradiance of the photobioreactor. Small green cells were widespread under lower light intensity, whereas big reddish cells were predominant under high light intensity. For these reasons, growth reflected by cell number or dry weight varied markedly with light conditions. Even under internal illumination of the photobioreactor, light penetration was significantly decreased as algal cells grew. Therefore, we employed a multistage process by gradually increasing the internal illuminations for astaxanthin production. Our results revealed that a multistage process might be essential to the successful operation of a photobioreactor for astaxnthin production using H. pluvialis.
Experimental Inhibition of Corneal Neovascularization by Photodynamic Therapy with Verteporfin
Yoon, Kyung-Chul,Ahn, Kyu,Lee, Song,Kim, Kyung,Im, Seong-Kyu,Oh, Han-Jin,Jeong, In-Young,Park, Sang-Woo,Park, Yeoung-Geol,Nah, Hyeon-Ju,Im, Wook-Bin IRL Press 2006 Current eye research Vol.31 No.3
<P>Purpose : To investigate the anti-angiogenic effects of photodynamic therapy with verteporfin in a rabbit model of corneal neovascularization. Methods : One week after suturing, the localization of verteporfin in the neovascularized cornea was examined through fluorescent microscopy 1 hr after administration. Rabbits were treated with one or two times of photodynamic therapy with verteporfin at 1-week intervals. Analysis of corneal neovascularization was performed by biomicroscopic and histological examinations. Results : Fluorescent microscopy showed green fluorescence in the vascular walls and interstitial tissue of the corneal stroma. The mean percentages of neovascularized corneal area at 3 days, 1 week, and 2 weeks after one time of photodynamic therapy were 90.3% ± 3.5%, 71.6% ± 6.2%, and 43.6% ± 15.1% in treated eyes and 96.4% ± 1.9% (p = 0.10), 88.6% ± 4.6% (p = 0.01), and 76.8% ± 4.4% (p</P>
Interleukin-6 and Tumor Necrosis Factor-α Levels in Tears of Patients With Dry Eye Syndrome
Yoon, Kyung-Chul,Jeong, In-Young,Park, Yeoung-Geol,Yang, Sung-Yeul Lippincott Williams Wilkins, Inc. 2007 Cornea Vol.26 No.4
PURPOSE:: To determine the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in tears of patients with dry eye syndrome. METHODS:: IL-6 and TNF-α levels were measured by enzyme-linked immunosorbent assay in tear samples obtained from 18 patients with dry eye (8 patients with Sjögren syndrome and 10 patients with non-Sjögren syndrome) and 14 control subjects. The correlation between IL-6 and TNF-α levels and tear film and ocular surface parameters was analyzed. The relative expression of these cytokines was evaluated in conjunctival impression cytology and conjunctival biopsy specimens by using immunohistochemical staining. RESULTS:: The mean levels of IL-6 and TNF-α were, respectively, 18.57 ± 8.92 and 3.68 ± 3.45 pg/mL in patients with dry eye and 3.59 ± 3.38 (P < 0.01) and < 0.5 (P < 0.01) pg/mL in control subjects. IL-6 level was significantly increased in tears of patients with Sjögren syndrome compared with those with non-Sjögren syndrome (P < 0.01). IL-6 level correlated significantly with tear film breakup time (P = 0.04), Schirmer test (P < 0.01), tear clearance (P = 0.02), keratoepithelioplasty score (P < 0.01), and goblet cell density (P = 0.03), but not with corneal sensitivity (P = 0.08). There was no significant difference in TNF-α level between patients with non-Sjögren and Sjögren syndrome. TNF-α levels did not correlate with tear film and ocular surface parameters. Immunohistochemical staining showed positive staining for IL-6 in specimens from patients with dry eye, especially in specimens from patients with Sjögren syndrome. CONCLUSION:: IL-6 and TNF-α levels are elevated in tears of patients with dry eye syndrome. IL-6 level, but not TNF-α level, is associated with the severity of the disease and correlates with various tear film and ocular surface parameters.
Sung Il Yoon,Minhee Han,Wei Wei,Haribandhu Chaudhuri,Yeoung-Sang Yun 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.126 No.-
Sustainable cost-effective activated charcoal-carboxymethyl cellulose (AC-CMC) fiber was developedthrough a benign method for high-rate water-softening applications. The as-developed fiber was characterizedusing FTIR, BET, stereomicroscope, XRD, and FESEM. The fiber was not only used for the removalof hardness (adsorptions of Ca(II) and Mg(II)) from different aqueous media but also successfully appliedin a membrane system. The effect of pH, contact time, isotherm, and kinetic experiments were conductedto examine the sorption efficiency of the fiber. The fiber showed an adsorption efficacy of 46.1 mg/g andfast rate kinetics of 0.1521 L/min for Ca(II). The results were compared to that of the commercial ionexchangefibers and beads namely Fiban K-1 and 001X8 NA, respectively. The experimental results fittedthe best with the Langmuir isotherm and pseudo-first-order kinetic models. It can be assumed that theelectrostatic and dipole-ion interactions between the fibers and Ca(II)/Mg(II) played a vital role duringthe sorption process. The fiber also showed proficient reusability performance. The developed fiberwas applied in the membrane system and it showed high breakthrough bed volume within a short residencetime of 25.7 seconds. So, this work demonstrates the significant potential of the AC-CMC fiber forhigh-rate water-softening application in the membrane system.