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인터넷을 이용한 온라인 디지털 상품 판매 및 전송 프로토콜
안은미(Eun-mi Ahn),이은성(Eun-sung Lee),류재철(Jae-cheol Ryou) 한국정보과학회 1998 한국정보과학회 학술발표논문집 Vol.25 No.1A
전자 상거래가 활성화되기 위해서 판매자나 구매자에게 더욱 안전하고 편리한 구매 및 판매 수단을 제공해 주어야 한다. 디지털 상품을 위한 판매 시스템으로 미국의 Open Market에서 개발한 시스템이 있다. 이 시스템은 인증 기능은 있으나 지불 뒤 상품의 암호화된 다운로드는 지원하지 않고 있어 해킹의 위험성을 안고 있다. 본 논문에서는 이를 개선하여 지불 뒤 안전한 다운로드 방법을 제안함으로써 상품 브라우징, 주문, 지불, 전송까지 모두 온라인으로 처리할 수 있는 안전한 프로토콜을 설계하고자 한다.
Kim, Yu Jin,Ryou, Sang-Mi,Kim, Sudeok,Yeom, Ji-Hyun,Han, Min Su,Lee, Kangseok,Seong, Maeng-Je The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.48
<P>The measurement of the binding force between a cell membrane and an oligonucleotide-functionalized, gold-coated tip using atomic force microscope force spectroscopy showed enhanced binding forces due to the presence of proteins in the buffer. The cellular uptake of oligonucleotide–gold nanoparticle composites was also enhanced when the composites were coated with serum proteins. Confocal microscopy image analysis of fluorescently labeled serum proteins and the oligonucleotides of the composites revealed co-transport of proteins and the composites.</P> <P>Graphic Abstract</P><P>Protein-mediated binding enhancement between oligonucleotide–gold nanoparticle composites and cell surfaces facilitates co-transport of proteins and composites. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm34047j'> </P>
Jong-Myung Kim,신은경,SANG-MI RYOU,JI-HYUN YEOM,이강석 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.4
One of the key challenges in the experimental and therapeutic use of gene delivery agents is the development of methods that can efficiently deliver nucleic acids into living systems. During the past decade, the development of effective and safe gene delivery systems has been intensively investigated. This review summarizes the current state of gene delivery methods based on viral and non-viral agents.
Kim, Hong-Man,Ryou, Sang-Mi,Song, Woo-Seok,Sim, Se-Hoon,Cha, Chang-Jun,Han, Seung Hyun,Ha, Nam-Chul,Kim, Jae-Hong,Bae, Jeehyeon,Cunningham, Philip R.,Lee, Kangseok American Society for Microbiology 2009 Journal of Bacteriology Vol.191 No.7
<B>ABSTRACT</B><P>Previous studies identified G791 in <I>Escherichia coli</I> 16S rRNA as an invariant residue for ribosome function. In order to establish the functional role of this residue in protein synthesis, we searched for multicopy suppressors of the mutant ribosomes that bear a G-to-U substitution at position 791. We identified <I>relA</I>, a gene whose product has been known to interact with ribosomes and trigger a stringent response. Overexpression of RelA resulted in the synthesis of approximately 1.5 times more chloramphenicol acetyltransferase (CAT) protein than could be synthesized by the mutant ribosomes in the absence of RelA overexpression. The ratio of mutant rRNA to the total ribosome pool was not changed, and the steady-state level of CAT mRNA was decreased by RelA overexpression. These data confirmed that the phenotype of RelA as a multicopy suppressor of the mutant ribosome did not result from the enhanced synthesis of mutant rRNA or CAT mRNA from the plasmid. To test whether the phenotype of RelA was related to the stringent response induced by the increased cellular level of (p)ppGpp, we screened for mutant RelA proteins whose overexpression enhances CAT protein synthesis by the mutant ribosomes as effectively as wild-type RelA overexpression and then screened for those whose overexpression does not produce sufficiently high levels of (p)ppGpp to trigger the stringent response under the condition of amino acid starvation. Overexpression of the isolated mutant RelA proteins resulted in the accumulation of (p)ppGpp in cells, which was amounted to approximately 18.2 to 38.9% of the level of (p)ppGpp found in cells that overexpress the wild-type RelA. These findings suggest that the function of RelA as a multicopy suppressor of the mutant ribosome does not result from its (p)ppGpp synthetic activity. We conclude that RelA has a previously unrecognized role in ribosome function.</P>
Functional Analysis of the Invariant Residue G791 of Escherichia coli 16S rRNA
송우석,김홍만,김재홍,심세훈,Sang-Mi Ryou,Sanggoo Kim,차창준,Philip R. Cunningham,배지현,이강석 한국미생물학회 2007 The journal of microbiology Vol.45 No.5
The nucleotide at position 79 (G791) of E. coli 16S rRNA was previously identified as an invariant residue for ribosomal function. In order to characterize the functional role of G791, base substitutions were introduced at this position, and mutant ribosomes were analyzed with regard to their protein synthesis ability, via the use of a specialized ribosome system. These ribosomal RNA mutations attenuated the ability of ribosomes to conduct protein synthesis by more than 65%. A transition mutation (G to A) exerted a moderate effect on ribosomal function, whereas a transversion mutation (G to C or U) resulted in a loss of protein synthesis ability of more than 90%. The sucrose gradient profiles of ribosomes and primer extension analysis showed that the loss of protein-synthesis ability of mutant ribosomes harboring a base substitution from G to U at position 791 stems partially from its inability to form 70S ribosomes. These findings show the involvement of the nucleotide at position 791 in the association of ribosomal subunits and protein synthesis steps after 70S formation, as well as the possibility of using 16S rRNA mutated at position 791 for the selection of second-site revertants in order to identify ligands that interact with G791 in protein synthesis.
The effect of a healthy school tuck shop program on the access of students to healthy foods
Kirang Kim,Seo Ah Hong,Sung Ha Yun,Hyun Joo Ryou,Sang Sun Lee,Mi Kyung Kim 한국영양학회 2012 Nutrition Research and Practice Vol.6 No.2
The objective of this study was to evaluate the effect of a healthy school tuck shop program, developed as a way of creating a healthy and nutritional school environment, on students’ access to healthy foods. Five middle schools and four high schools (775 students) participated in the healthy school tuck shop program, and nine schools (1,282 students) were selected as the control group. The intervention program included restriction of unhealthy foods sold in tuck shops, provision of various fruits, and indirect nutritional education with promotion of healthy food products. The program evaluation involved the examination of students’ purchase and intake patterns of healthy foods, satisfaction with the available foodstuffs, and utilization of and satisfaction with nutritional educational resources. Our results indicated that among of the students who utilized the tuck shop, about 40% purchased fruit products, showing that availability of healthy foods in the tuck shop increased the accessibility of healthy foods for students. Overall food purchase and intake patterns did not significantly change during the intervention period. However, students from the intervention schools reported higher satisfaction with the healthy food products sold in the tuck shop than did those from the control schools (all P < 0.001), and they were highly satisfied with the educational resources provided to them. In conclusion, the healthy school tuck shop program had a positive effect on the accessibility of healthy food. The findings suggest that a healthy school tuck shop may be an effective environmental strategy for promoting students’ access to healthy foods.