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Stabilization of Bacillus subtilis Lipase A by increasing the residual packing
Abraham1, Tajo,Pil Pack1, Seung,Je Yoo, Young Taylor Francis 2005 Biocatalysis and biotransformation Vol.23 No.3
<P>Introduction of well-packed residues to the interior of a protein structure could be considered as a stabilization strategy since the reduction of buried cavities might stabilize protein structure. In this study, the less-packed residues with no water-contact were selected as target sites for increasing residual packing. When Lipase A from Bacillus subtilis (179 amino acids) was used as a model system, 43 less-packed residues were initially considered by analyzing their residual packing value and residual exposure ratio. Among the 43 residues, small amino acids such as GLY and ALA were chosen as target sites. Packing increases of ALA to VAL and GLY to ALA were estimated, by molecular modeling, to give 0.5368∼⃒0.7433  kcal mol −1 stabilization. Mutants of Lipase A such as A38V, A75V, G80A, A105V A146V, and G172A were obtained via protein engineering. Thermostability assays revealed that A38V, G80A and G172V were the most stable mutants. This procedure for selecting the target residues for improved thermostability of Lipase A could be applied for improving the thermostability of other proteins and enzymes.</P>
최경덕(Kyeongduck Choi),유상길(Sanggil Yoo),강희선(Heesun Kang),박상민(Sangmin Pack) 한국자동차공학회 2007 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-
This paper discusses on development of a BMS(Battery Management System) for D-HEV(Diesel-Hybrid Electronic Vehicle) including algorithm of battery(Lithium) SOC(State of Charge) estimation. The algorithm accurately estimates the remaining battery capacity for automated battery management. In this paper, a BMS including technique for battery monitoring(Pack-Voltage, Pack-Current, Cell-Voltage, Pack-Temperature), communication method, digital input/output control, a battery thermal control, and battery protection method is developed.
백민식(Min-Sik Pack),이시연(Si-Yeon Lee),양보석(Bo-Suk Yang),최성철(Sung-Choul Choi),이재근(Jae-Geun Lee) 대한기계학회 2004 대한기계학회 춘추학술대회 Vol.2004 No.11
This paper presents a control plan of clearances for power plant steam turbine with the characteristics of internal clearances. Internal clearances on the interstage of blades and shaft gland seals on the steam turbine which are installed in tandem compound was changed when the rotor turned in the cylindrical sleeve bearing due to the generation of oil film wedge. This presented concern is very useful to prevent the rubbing damage of seal edge between the fixed and moving parts in steam turbine due to the misalignment at the rotating and stationary parts. This method is applied for the unbalanced clearances distribution to the left and right sides in the turbine casing. A considerable amount of unbalanced clearances distribution trend is determined according to the rotating speed of shaft, bearing size, type of journal bearing, bearing load, lubrication oil viscosity, oil temperature, oil pressure and surface roughness of shaft.
An Adaptive Power Saving Mechanism in IEEE 802.11 Wireless IP Networks
Pack Sangheon,Choi Yanghee The Korea Institute of Information and Commucation 2005 Journal of communications and networks Vol.7 No.2
Reducing energy consumption in mobile hosts (MHs) is one of the most critical issues in wireles/mobile networks. IP paging protocol at network layer and power saving mechanism (PSM) at link layer are two core technologies to reduce the energy consumption of MHs. First, we investigate the energy efficiency of the current IEEE 802.11 power saving mechanism (PSM) when IP paging protocol is deployed over IEEE 802.11 networks. The result reveal that the current IEEE 802.11 PSM with a fixed wakeup interval (i.e., the static PSM) exhibits a degraded performance when it is integrated with IP paging protocol. Therefore, we propose an adaptive power saving mechanism in IEEE 802.11-based wireless IP networks. Unlike the static PSM, the adaptive PSM adjusts the wake-up interval adaptively depending on the session activity at IP layer. Specifically, the MH estimates the idle periods for incoming sessions based on the exponentially weighted moving average (EWMA) scheme and sets its wake-up interval dynamically by considering the estimated idle period and paging delay bound. For performance evaluation, we have conducted comprehensive simulations and compared the total cost and energy consumption, which are incurred in IP paging protocol in conjunction with various power saving mechanisms: The static PSM, the adaptive PSM, and the optimum PSM. Simulation results show that the adaptive PSM provides a closer performance to the optimum PSM than the static PSM.