Stabilization of Bacillus subtilis Lipase A by increasing the residual packing

Abraham1, Tajo,Pil Pack1, Seung,Je Yoo, Young Taylor Francis 2005 Biocatalysis and biotransformation Vol.23 No.3

<P>Introduction of well-packed residues to the interior of a protein structure could be considered as a stabilization strategy since the reduction of buried cavities might stabilize protein structure. In this study, the less-packed residues with no water-contact were selected as target sites for increasing residual packing. When Lipase A from Bacillus subtilis (179 amino acids) was used as a model system, 43 less-packed residues were initially considered by analyzing their residual packing value and residual exposure ratio. Among the 43 residues, small amino acids such as GLY and ALA were chosen as target sites. Packing increases of ALA to VAL and GLY to ALA were estimated, by molecular modeling, to give 0.5368∼⃒0.7433  kcal mol −1 stabilization. Mutants of Lipase A such as A38V, A75V, G80A, A105V A146V, and G172A were obtained via protein engineering. Thermostability assays revealed that A38V, G80A and G172V were the most stable mutants. This procedure for selecting the target residues for improved thermostability of Lipase A could be applied for improving the thermostability of other proteins and enzymes.</P>

디젤하이브리드 배터리 관리 시스템 개발

최경덕(Kyeongduck Choi),유상길(Sanggil Yoo),강희선(Heesun Kang),박상민(Sangmin Pack) 한국자동차공학회 2007 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

This paper discusses on development of a BMS(Battery Management System) for D-HEV(Diesel-Hybrid Electronic Vehicle) including algorithm of battery(Lithium) SOC(State of Charge) estimation. The algorithm accurately estimates the remaining battery capacity for automated battery management. In this paper, a BMS including technique for battery monitoring(Pack-Voltage, Pack-Current, Cell-Voltage, Pack-Temperature), communication method, digital input/output control, a battery thermal control, and battery protection method is developed.

Quantitative analysis of specific target DNA oligomers using a DNA-immobilized packed-column system

Pack, Seung Pil,Heo, Tae-Hwe,Devarayapalli, Kamakshaiah Charyulu,Makino, Keisuke Springer-Verlag 2011 ANALYTICAL AND BIOANALYTICAL CHEMISTRY Vol.401 No.2

발전용 증기터빈 내부간극 관리방안

백민식(Min-Sik Pack),이시연(Si-Yeon Lee),양보석(Bo-Suk Yang),최성철(Sung-Choul Choi),이재근(Jae-Geun Lee) 대한기계학회 2004 대한기계학회 춘추학술대회 Vol.2004 No.11

This paper presents a control plan of clearances for power plant steam turbine with the characteristics of internal clearances. Internal clearances on the interstage of blades and shaft gland seals on the steam turbine which are installed in tandem compound was changed when the rotor turned in the cylindrical sleeve bearing due to the generation of oil film wedge. This presented concern is very useful to prevent the rubbing damage of seal edge between the fixed and moving parts in steam turbine due to the misalignment at the rotating and stationary parts. This method is applied for the unbalanced clearances distribution to the left and right sides in the turbine casing. A considerable amount of unbalanced clearances distribution trend is determined according to the rotating speed of shaft, bearing size, type of journal bearing, bearing load, lubrication oil viscosity, oil temperature, oil pressure and surface roughness of shaft.

KREUZ UND ORDO BEI OTFRID : Interpretation des Kapitels V, I

PACK, Sang Be 同德女子大學校 1993 同大論叢 Vol.23 No.1

오트프리트 폰 바이쎈부르크 Otfrid von Weissenburg는 9세기 후반기에 6천여행에 달하는 대서사시 Evangelienbuch를 쓴 독일의 시인이다. 그는 두운법 Stabreim으로 일관되어 왔던 게르만의 시문학을 극복하여 각운법 Endreim을 독일에서 최초로 이 서사시에 도입하여 성공을 거두었다는 점에서 독일시 운률사에 획기적 전환점을 마련했다고 평가 받고 있다. 본 논문은 여러 독일 중세연구가들의 연구물들을 토대로하여 이 대서사시(5부로 형성되었음)의 구성 Komposition에 있어 제기된 문제점, 특히 수리상징 Zahlensymbolik과 그 해석의 논쟁점을 정리, 소개하면서 재검토해 보는 형식을 취했다. 그것도 작품의 전반에 대한 검토에 앞서 우선 제5서 1장을 주대상으로 하여 그 경계를 한정할 수 밖에 없었다. 주로 이 상징해석에 적극적인 태로를 견지한 학자들의 입장에 주안점을 둔 것이며, 이에 비판적인 학자들의 입장에 대해서는 차후 따로 정리, 그 대안점을 구체적으로 밝혀 놓을 생각이다. Johannes Rathofer가 1962년 이 해석의 가능성을 천명한 이래 이에 대한 찬반양론은 학계의 큰 관심사로 머물고 있기 때문이다.

An Adaptive Power Saving Mechanism in IEEE 802.11 Wireless IP Networks

Pack Sangheon,Choi Yanghee The Korea Institute of Information and Commucation 2005 Journal of communications and networks Vol.7 No.2

Reducing energy consumption in mobile hosts (MHs) is one of the most critical issues in wireles/mobile networks. IP paging protocol at network layer and power saving mechanism (PSM) at link layer are two core technologies to reduce the energy consumption of MHs. First, we investigate the energy efficiency of the current IEEE 802.11 power saving mechanism (PSM) when IP paging protocol is deployed over IEEE 802.11 networks. The result reveal that the current IEEE 802.11 PSM with a fixed wakeup interval (i.e., the static PSM) exhibits a degraded performance when it is integrated with IP paging protocol. Therefore, we propose an adaptive power saving mechanism in IEEE 802.11-based wireless IP networks. Unlike the static PSM, the adaptive PSM adjusts the wake-up interval adaptively depending on the session activity at IP layer. Specifically, the MH estimates the idle periods for incoming sessions based on the exponentially weighted moving average (EWMA) scheme and sets its wake-up interval dynamically by considering the estimated idle period and paging delay bound. For performance evaluation, we have conducted comprehensive simulations and compared the total cost and energy consumption, which are incurred in IP paging protocol in conjunction with various power saving mechanisms: The static PSM, the adaptive PSM, and the optimum PSM. Simulation results show that the adaptive PSM provides a closer performance to the optimum PSM than the static PSM.

Graph-und Lautzustande das Textes

PACK, Sang Be(朴尙培) 부산대학교 인문학연구소 1986 코기토 Vol.30 No.-

STUDIEN ZUM MITTELHOCHDEUTSCHEN SCHWANK(I)

PACK,Sang Be 同德女子大學校 1992 同大論叢 Vol.22 No.1

유럽의 골계문학은 9세기에서 10세기에 걸쳐 독일과 불란서에서 라틴어로 씌어진 Schwank 작품들이 등장하면서 시작된다. 이들은 대개 고대 Antike나 근동 Orient에서 소재나 모티브를 수용할 것으로 중세의 음유가인의 시가 Spielmannsdichtung에서 이미 그 변용의 형태가 보이고, 십자군 원정으로 활발한 모티브 교환이 있었음을 알 수 있다. 13세기경 Stricker에 의한 불란서 우화의 수용, 5세기경 독일적 골계인물의 형성을 거쳐 16세기에 Hans Sachs에 의해 본격적 골계문학이 전개된다. 17세기에는 Grimmelshausen, 18세기에는 G. A. Burger, J. H. Voss, J. p. Hebel등에 의해 이 장르가 발전되었고 19세기에 G. Keller의 Schildburgertum에서 그 정점에 이른다. 본 논문은 독일골계문학의 뿌리역을 맡고 있는 중세의 두개의 Schwank작품을 우선 택하여 그것의 발생, 전래, 간행물(서지), 소재사, 주제사, 구성, 양식, 내용 등을 중심으로 하여 제 학자들의 연구와 그 논쟁점을 재고함과 아울러 비판해 본 것이다. 이 작업은 중고지독어로 씌어진 골계문학 전반의 연구를 위한 그 일단계가 된다.

A Molecular Framework for Risk Assessment of a Virus-Tolerant Transgenic Pepper Line

Pack, In-Soon,Kim, Yu-Jin,Youk, Eun-Soo,Lee, Woo-Kyu,Yoon, Won-Kee,Park, Kee-Woong,Kim, Chang-Gi,Harn, Chee-Hark,Kim, Hwan-Mook,Jeong, Soon-Chun 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.2

The Cucumber mosaic virus coat protein (CMV-CP) gene-transgenic pepper lines exhibit high tolerance to Cucumber mosaic virus (CMV) strains. In this study, E7, one of the CMVP0-CP transgenic chili pepper events selected by screening was further characterized. Southern blotting and inverse PCR analysis revealed that the E7 event contains a single copy of the inserted gene cassette whose flanking sequences appear to be noncoding and intergenic. We searched for pepper-specific DNA sequence candidates as an endogenous reference gene for GM-pepper detection. We found that only one copy of CaSIG4 and lipocalin genes are present in the pepper genome and their sequences were determined to be pepper-specific. The characterization of the genomic sequences flanking the transgene, as well as the availability of the pepper-specific single copy CaSIG4 and lipocalin genes as endogenous reference genes, enabled the design of E7-event-specific PCR-based quantitative detection methods. The CMV-CP protein levels in the CMV-inoculated wild-type pepper tissues were approximately 60 times higher than those in the uninoculated and CMV-inoculated E7 pepper tissues. These results suggested that the amount of CMV-CP expressed in transgenic pepper tissue was negligible relative to the amount of CMV-CP in the virus-infected wild-type pepper consumed by human beings. This work may prove useful for risk assessment studies of transgenic pepper lines. Furthermore, the characterized single copy genes, lipocalin and CaSIG4, may be used to develop a method to detect gene copy number variations in the pepper genome.

Silica - Pillared H-magadiites : Interlamellar Base - Catalyzed Hydrolysis of Tetraethylorthosilicate in Pure Water Suspension

Pack, U Hyon,Kwon, Oh Yun,Park, Kyeong Won 한국공업화학회 1999 Journal of Industrial and Engineering Chemistry Vol.5 No.2

The silica-pillared H^+-magadiites were prepared by interlamellar base-catalyzed hydrolysis of tetraethylorthosilicate [TEOS, Si(OC₂H_5)₄] intercalated into the interlayers of H^+-magadiite. The interlamellar hydrolysis of TEOS were conducted between 10 min to 40 min in 0.00%, 0.05%, 0.10% and 0.20% NH₃-water solution, affording siloxane-pillared H^+-magadiites with gallery height of 24.8∼30.3Å. Calcination of samples at 538℃ in air resulted in silica-pillared H^+-magadiites with a large specific surface areas between 551 and 1020 ㎡/g, depending on the aging time and NH₃ concentration. The reaction in pure water and 0.05% NH₃ solution afforded optimum specific surface areas and mesopores with narrow pore size distribution. The reaction in 0.10% and 0.20% NH₃ solution resulted in sharp decrease of specific surface areas and poorly-ordered basal spacings, implying the partial destruction in the layered structure of H^+-magadiite.