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On-Sook Hur,Sang Gyu Kim,Ho-Cheol Ko,Su Ran Ahn,Jung-Sook Sung,Na-Young Ro,Sukyeung Lee,Yu-mi Choi,Do yoon Hyun,Kyoung-Yul Ryu,Hyung-Jin Baek 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
This study aimed to evaluate 105 tomato accessions conserved in National Agrobiodiversity Center regarding their resistance to Ralstonia solanacearum, a soil-borne vascular bacterium that causes lethal wilt diseases of a wide range of crops worldwide. All the accessions are Solanum lycopericum var. lycopersicum including cultivar or breeding lines. At the four leaf stage, the seedlings were inoculated by drenching the soil with the bacterial suspension concentrated of 108 CFU/ml. Plant roots were wounded before inoculation by cutting with the knife. Seven accessions including IT 32899 were rated as resistant, while other 98 accessions were rated as susceptible. IT 32899 scored 0.1 of disease rate and 0.7 of disease index. The selected accessions will be used as a material to reveal the mechanism of wilt tolerance and to identify the host gene involved in defense response.
Comparison of Glucosinolates Contents in the Germplasm of Different Cruciferous Vegetables
On-Sook Hur,Jung-Bong Kim,Jung-Sook Sung,Sang Gyu Kim,Jae-Gyun Gwak,Sukyeung Lee,Yu-mi Choi,Do yoon Hyun,Myung-chul Lee,Hyung-Jin Baek,Binod Prasad Luitel,Kyoung-Yul Ryu,Ho-Cheol Ko 한국원예학회 2015 한국원예학회 학술발표요지 Vol.2015 No.10
Hur, On-Sook,Chang, Dong-Chil,Kim, Sun-Lim,Ok, Hyun-Chung,Kim, Jung-Tae,Chun, Chang-Hoo The Plant Resources Society of Korea 2011 한국자원식물학회지 Vol.24 No.6
The composition of free sugar, free amino acid, and fatty acid in tubers of the cultivars 'Superior', 'Sinnamjak', and 'Chubaek' were evaluated at the two sites with different altitude, Gangneung (20 m altitude) as lowland and Daekwallyeong (760 m altitude) as highland. The average reducing sugar content of the three potato cultivars harvested in Daekwallyeong and Gangneung was 1.1% and 2.3%, respectively, which showed two-fold difference between the two locations. Average total sugar content was also two-folds lower in potatoes harvested in Daekwallyeong (6.3%) than those harvested in Gangneung (12.2%). Average content of free amino acids of the three potato cultivars harvested in Daekwallyeong and Gangneung was 1,325 mg/100g and 1,051 mg/100g, respectively. The cultivar 'Chubaek' has the highest amino acid content among the three tested cultivars. Potatoes from Daekwallyeong have higher unsaturated fatty acid levels than those from Gangneung. During the tuber development, unsaturated fatty acids levels increased in tubers grown in Daekwallyeong, but decreased in those grown in Gangneung. These results indicate that the quality of potatoes from Daekwallyeong is from Gangneung for food processing purpose for human consumption.
Screening of Resistance to Gray Leaf Spot in Tomato Germplasm
On-Sook Hur,Bora Geum,Na-Young Ro 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The resistance of tomato (Solanum lycopersicum) to the gray leaf spot disease caused by Stemphylium species is conferred by a single incompletely dominant gene, Sm. The resistance gene was introgressed into cultivars from the wild species S. pimpinellifolium on chromosome 11. To select resistant genetic resources to gray leaf spot, we have screened 200 tomato accessions conserved at National Agrobiodiversity Center by inoculum with gray leaf spot. Also, we have genotyped with the marker, Sm_D3 positioned in Solyc11g018720. Seventeen accessions including IT173883 were resistant to Stemphyllium sp. developing necrotic local regions at inoculated leaves as primary symptoms. Selected accessions of S. pimpinellifolium will hopefully contribute for use as donor parents for breeding programs with a new resistant source of gray leaf spot in tomato commercial varieties.
Evaluation of 200 Tomato Wild Accessions for Resistance to Tomato Spotted Wilt Virus (TSWV)
On-Sook Hur,Na-Young Ro,Sukyeong Lee,Aejin Hwang,Seonghoon Kim,Bich-Saem Kim,Awraris Derbie Assefa,Bum-Soo Hahn 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Tomato spotted wilt virus (TSWV) is an economically damaging pathogen of tomatoes. Typical symptoms of TSWV include wilting or stunning plants and chlorotic or necrotic rings on leaves and fruits. The effective management for TSWV is breeding tomato cultivars containing the Sw-5 from an unknown Solanum peruvianum. Unfortunately, several Sw-5 resistance-breaking strains of TSWV have been identified. Sw-7 from S. chilense accession LA1938 is an alternative locus conferring resistance to TSWV. The objective of this study was to find tolerant or resistant germplasm to TSWV. A total of 200 tomato wild accessions were evaluated for the resistance to TSWV using a bioassay with strain TSWV-SW-TO2. IT173785 (S. peruvianum) and 5 accessions of S. pimpinellifolium including TI173719 were showed apparent recovery or no symptom on upper leaves at 4 weeks later after inoculation. We will conduct the high resolution DNA melting analysis (HRM) with Sw-5 and Sw-7. These selected accessions will hopefully contribute for new source of resistance in tomato for protection against TSWV.