Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

Factors influencing choosing teaching as a career: South Korean preservice teachers

Jeong‑Ae Lee,Mi Ok Kang,Bitnara Jasmine Park 서울대학교 교육연구소 2019 Asia Pacific Education Review Vol.20 No.3

This study validated the underlying factor structure of the Factors Influencing Teaching (FIT)-Choice survey in the South Korea context and examined motivations and perceptions of 903 Korean preservice teachers by major demographic characteristics. Secondary preservice teachers reported significantly higher values than elementary preservice teachers for Qualification fit and desire to teach and Task return. Male preservice teachers believed more strongly than female preservice teachers that they chose the teaching profession because of Qualification fit, Influencing others, and Task return. Compared to freshmen/sophomores, juniors/seniors reported significantly higher values for Task demand. Participants with low- to medium-level self-reported income ($12K–$46K) weighted Benefits significantly higher than those with medium-level income ($46K–$88K). STEM majors reported higher values for the Qualification fit and Influencing others than non-STEM majors. As South Korea is one of the few countries which has an oversupply of highly qualified teacher candidates, findings were compared with other countries to provide useful insights to improve recruitment of highly qualified individuals to teaching workforce in many countries where teacher shortage is a chronic problem. Based on these data analyses, we argue that policy makers, politicians, education researchers, and others who care about quality education and student academic achievements need to make efforts to increase task returns and benefits.

Characterization of Mycoplasma gallisepticum isolates from chicken in South Korea

Ok-Mi Jeong(Ok-Mi Jeong),Jin-Hyun Kim(Jin-Hyun Kim),So-Youn Youn(So-Youn Youn),Hye-Jin Lee(Hye-Jin Lee),Min-Su Kang(Min-Su Kang) 한국예방수의학회 2017 한국예방수의학회 학술대회자료집 Vol.2017 No.-

Ichthyotoxic Cochlodinium polykrikoides red tides offshore in the South Sea, Korea in 2014: I. Temporal variations in three-dimensional distributions of red-tide organisms and environmental factors

Jeong, Hae Jin,Lim, An Suk,Lee, Kitack,Lee, Moo Joon,Seong, Kyeong Ah,Kang, Nam Seon,Jang, Se Hyeon,Lee, Kyung Ha,Lee, Sung Yeon,Kim, Mi Ok,Kim, Ji Hye,Kwon, Ji Eun,Kang, Hee Chang,Kim, Jae Seong,Yih, The Korean Society of Phycology 2017 ALGAE Vol.32 No.2

The ichthyotoxic Cochlodinium polykrikoides red tides have caused great economic losses in the aquaculture industry in the waters of Korea and other countries. Predicting outbreak of C. polykrikoides red tides 1-2 weeks in advance is a critical step in minimizing losses. In the South Sea of Korea, large C. polykrikoides red tide patches have often been recorded offshore and transported to nearshore waters. To explore the processes of offshore C. polykrikoides red tides, temporal variations in 3-dimensional (3-D) distributions of red tide organisms and environmental parameters were investigated by analyzing 4,432 water samples collected from 2-5 depths of 60 stations in the South Sea, Korea 16 times from May to Nov, 2014. In the study area, the vegetative cells of C. polykrikoides were found as early as May 7, but C. polykrikoides red tide patches were observed from Aug 21 until Oct 9. Cochlodinium red tides occurred in both inner and outer stations. Prior to the occurrence of large C. polykrikoides red tides, the phototrophic dinoflagellates Prorocentrum donghaiense (Jun 12 to Jul 11), Ceratium furca (Jul 11 to Aug 21), and Alexandrium fraterculus (Aug 21) formed red tides in sequence, and diatom red tides formed 2-3 times without a certain distinct pattern. The temperature for the optimal growth of these four red tide dinoflagellates is known to be similar. Thus, the sequence of the maximum growth rates of P. donghaiense > C. furca > A. fraterculus > C. polykrikoides may be partially responsible for this sequence of red tides in the inner stations following high nutrients input in the surface waters because of heavy rains. Furthermore, Cochlodinium red tides formed and persisted at the outer stations when $NO_3$ concentrations of the surface waters were < $2{\mu}M$ and thermocline depths were >20 m with the retreat of deep cold waters, and the abundance of the competing red-tide species was relatively low. The sequence of the maximum swimming speeds and thus potential reachable depths of C. polykrikoides > A. fraterculus > C. furca > P. donghaiense may be responsible for the large C. polykrikoides red tides after the small blooms of the other dinoflagellates. Thus, C. polykrikoides is likely to outgrow over the competitors at the outer stations by descending to depths >20 m and taking nutrients up from deep cold waters. Thus, to predict the process of Cochlodinium red tides in the study area, temporal variations in 3-D distributions of red tide organisms and environmental parameters showing major nutrient sources, formation and depth of thermoclines, intrusion and retreat of deep cold waters, and the abundance of competing red tide species should be well understood.

Development of a Rapid Detection Kit for H9 Specific Avian Influenza Virus

Ok Mi Jeong,Min Chul Kim,Hyun Mi Kang,Gun Woo Ha,Jin Sik Oh,Su Chul Lee,Kun Seok Na,Ji Sun Kwon,Mi Ra Pack,Jun Hun Kwon,Youn Jeong Lee 대한수의학회 2009 대한수의학회 학술대회발표집 Vol.2009 No.-

Efficacy of a Hierarchical Treatment of Aphasic Perseveration (TAP) Program : A Case Study

Jeong, Ok Ran,Lee, Sang Heun,Cho,Tae Hwan,Sohn, Jin Ho,Yeh, Mi Kyung 한국음성과학회 2001 음성과학 Vol.8 No.1

This paper explored the effectiveness of a hierarchical Treatment of Aphasic Pcr-Teveratiorx (TAP) program on an aphasic in two regards: decrease in the frequency of perseveration and improvement in naming ability. The subject was a 54-year-old female with Transcortical Sensory (TC) aphasia following a left ischemic crcbrovascular Accident (CVA). It was concluded that the hierarchical TAIL program was more effective and efficient in terms of decreasing perseveration and increasing correct naming performance. Within the data of the hierarchical TAP program, however, a substantial decrease in the frequency of occurrence of perseverative behaviors did not necessarily result in proportionate improvement of naming ability. The probable causes and reasons were discussed,

A Study of Insole Inserted Indoor Sandal Effectiveness in School Aged Patients with Flexible Flat-foot deformity

Jeong Eun Moon,Mi-Ok Song,Yong Jin Cho 한국간호과학회 2019 한국간호과학회 학술대회 Vol.2019 No.10

<i>Microbulbifer gwangyangensis</i> sp. nov. and <i>Microbulbifer pacificus</i> sp. nov., isolated from marine environments

Jeong, Sang Hyeon,Yang, Sung-Hyun,Jin, Hyun Mi,Kim, Jeong Myeong,Kwon, Kae Kyoung,Jeon, Che Ok International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.4

<P>Two novel Gram-stain-negative, chemoheterotrophic and strictly aerobic bacteria, strains GY2<SUP>T</SUP> and SPO729<SUP>T</SUP>, were isolated from a tidal flat at Gwangyang Bay in Korea and a marine sponge sample from the Pacific Ocean, respectively. The two strains were halotolerant, catalase- and oxidase-positive, and non-motile rods. Optimum temperature and pH for growth of both strains were observed to be 35 °C and pH 7.0–7.5, but optimum salinity for strain SPO729<SUP>T</SUP> [2–3 % (w/v)] was slightly higher than that for strain GY2<SUP>T</SUP> (1–2 %). The major cellular fatty acids of both strains were C<SUB>16 : 0</SUB>, iso-C<SUB>15 : 0</SUB>, iso-C<SUB>17 : 0</SUB>, iso-C<SUB>17 : 1</SUB>ω9<I>c</I>, C<SUB>18 : 1</SUB>ω7<I>c</I>, iso-C<SUB>11 : 0</SUB> and iso-C<SUB>11 : 0</SUB> 3-OH. The genomic DNA G+C contents of strains GY2<SUP>T</SUP> and SPO729<SUP>T</SUP> were 55.1 and 57.9 mol%, respectively, and ubiquinone 8 (Q-8) was detected as the sole respiratory quinone from the two strains. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains GY2<SUP>T</SUP> and SPO729<SUP>T</SUP> formed tight phyletic lineages with members of the genus <I>Microbulbifer</I>. Strain GY2<SUP>T</SUP> was closely related to <I>Microbulbifer okinawensis</I> ABABA23<SUP>T</SUP> (98.2 %), strain SPO729<SUP>T</SUP> (98.0 %) and <I>Microbulbifer donghaiensis</I> CN85<SUP>T</SUP> (97.0 %); strain SPO729<SUP>T</SUP> was closely related to <I>M. okinawensis</I> ABABA23<SUP>T</SUP> (98.3 %) and <I>M. donghaiensis</I> CN85<SUP>T</SUP> (98.2 %). The DNA–DNA relatedness values of strain GY2<SUP>T</SUP> with <I>M. okinawensis</I> ABABA23<SUP>T</SUP>, strain SPO729<SUP>T</SUP> and <I>M. donghaiensis</I> CN85<SUP>T</SUP> were 40.0±2.1 %, 13.1±3.9 % and 16.2±5.8 %, respectively, whereas those of strain SPO729<SUP>T</SUP> with <I>M. okinawensis</I> ABABA23<SUP>T</SUP> and <I>M. donghaiensis</I> CN85<SUP>T</SUP> were 48.0±4.0 % and 34.6±9.3 %, respectively. On the basis of phenotypic and molecular features, it is concluded that the two strains GY2<SUP>T</SUP> and SPO729<SUP>T</SUP> represent two novel species of the genus <I>Microbulbifer</I>, for which the names <I>Microbulbifer gwangyangensis</I> sp. nov. and <I>Microbulbifer pacificus</I> are proposed; the type strains are GY2<SUP>T</SUP> ( = KACC 16189<SUP>T</SUP> = JCM 17800<SUP>T</SUP>) and SPO729<SUP>T</SUP> ( = KCCM 42667<SUP>T</SUP> = JCM 14507<SUP>T</SUP>), respectively.</P>

Synergistic Sequential Reaction of Alginolytic Enzyme Complex for Efficient Brown Algae Degradation Activity

Mi Jung LEE,Jeong Eun HYEON,Da Woon JUNG,Sung Ok HAN 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.4

Text Network Analysis of Research Topics on Virtual Simulation in Nursing

Mi Ok Song,Aeri Jang,Jeong Eun Moon 한국간호과학회 2021 한국간호과학회 학술대회 Vol.2021 No.10

Aim(s): The purpose of this study is to examine the relationship between keywords on virtual simulation in nursing studies and to develop schematic diagrams of the relationship between these keywords from a macro perspective. It is to identify the trend of the literature on virtual simulation in nursing. Method(s): This was a text-mining study that collected, processes, analyzed text data. For this study, we searched several databases such as PubMed, CINAHL, EMBASE, Web of Science, SCOPUS from 1st~15th, July, 2021. The Inclusion criteria included the following: (a) peer-reviewed, (b) journal article, (c) written in English, (d) including a text ‘virtual’ or ‘virtual reality’, ‘simulation’, ‘education’, ‘learning’, ‘training’ in its each title, abstract and keywords. (e) with nursing students and nurses, (f) to 31, May, 2021. Exclusion criteria included following: (a) not journal articles (dissertations, conference proceedings, Book section et al. b) language besides English, c) not involved virtual simulation, d) participants that did not include nurses and nursing students. Thus, 570 studies were selected in the library databases. Among these data, duplicate (222), no abstract (8), unrelated studies (72) and not articles (63) were excluded. As a result, a total of 213 papers were extracted. The keywords from 213 articles were preprocessed and keyword analysis and topic modeling were performed using NetMiner 4.3 software. For topic modeling, Latent Dirichlet Allocation was applied. Result(s): As for virtual simulation in nursing, keywords with the most frequent appearance and the highest networking degree in centrality were ‘nurse’, ’skill’, ‘care’, ‘knowledge’, ‘performance’, ‘communication’, ‘disaster’, ‘experience’, ‘health’. A total of 2347 keywords were categorized into 10 topics: ‘dementia care competency’, ‘pain assessment’, ‘airway placement management’, ‘operating procedure’, ‘presence & satisfaction’, ‘communication & attitude improvement’, ‘platform world’, ‘disaster response’, ‘game usability’, ‘system for confidence’ Conclusion(s): The identified trends in this study will help nursing educators and researchers to grasp the trends and insight for virtual simulation in nursing and to guide future research directions on virtual simulation in nursing.