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Bacteriophage Usage for Bacterial Disease Management and Diagnosis in Plants
Nguyen Trung Vu,Chang-Sik Oh 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.3
In nature, plants are always under the threat of pests and diseases. Pathogenic bacteria are one of the ma- jor pathogen types to cause diseases in diverse plants, resulting in negative effects on plant growth and crop yield. Chemical bactericides and antibiotics have been used as major approaches for controlling bacterial plant diseases in the field or greenhouse. However, the appearance of resistant bacteria to common antibiot- ics and bactericides as well as their potential negative effects on environment and human health demands bacteriologists to develop alternative control agents. Bacteriophages, the viruses that can infect and kill only target bacteria very specifically, have been demon- strated as potential agents, which may have no negative effects on environment and human health. Many bac- teriophages have been isolated against diverse plant- pathogenic bacteria, and many studies have shown to efficiently manage the disease development in both controlled and open conditions such as greenhouse and field. Moreover, the specificity of bacteriophages to cer- tain bacterial species has been applied to develop detec- tion tools for the diagnosis of plant-pathogenic bacteria. In this paper, we summarize the promising results from greenhouse or field experiments with bacteriophages to manage diseases caused by plant-pathogenic bacteria. In addition, we summarize the usage of bacteriophages for the specific detection of plant-pathogenic bacteria.
Inhibitory Activity of Plantago major L. on Angiotensin I-converting Enzyme
Nguyen Xuan Nhiem,김영호,Bui Huu Tai,Phan Van Kiem,Chau Van Minh,Nguyen Xuan Cuong,Nguyen Huu Tung,Vu Kim Thu,Trinh Nam Trung,Hoang Le Tuan Anh,조성훈,장해동,권영인 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.3
Eight compounds were isolated from methanol extract of Plantago major L. leaves and investigated for their ability to inhibit angiotensin I-converting enzyme activity. Among them, compound 1 showed the most potent inhibition with rate of 28.06 ± 0.21% at a concentration of 100 μM. Compounds 2 and 8 exhibited weak activities. These results suggest that compound 1 might contribute to the ability of P. major to inhibit the activity of angiotensin I- converting enzyme.
Antibiotic Resistance of Pectobacterium Korean Strains Susceptible to the Bacteriophage phiPccP-1
Vu Nguyen Trung,Roh Eunjung,Thi Thuong Nguyen,Oh Chang Sik 한국식물병리학회 2022 식물병연구 Vol.28 No.3
Commercial products with antibiotics like streptomycin as active ingredients have been used to control soft rot disease caused by <i>Pectobacterium</i> species for a long time. In this study, antibiotic resistance of twentyseven Korean strains of <i>Pectobacterium</i> species including <i>P. carotovorum, P. odoriferum, P. brasiliense,</i> and <i>P. parmenteri,</i> which were previously shown to be susceptible to the bacteriophage phiPccP-1 was surveyed using a disk diffusion assay. While all strains were highly susceptible to ampicillin, kanamycin, chloramphenicol, tetracycline, and rifampicin, some strains showed weak susceptibility to 300 μg/ml of streptomycin. Furthermore, some of them are partially or completely resistant to commercial pesticides—Buramycin and streptomycin at the concentration of 250 μg/ml that is recommended by the manufacturer for streptomycin- based pesticides. These results indicate the presence of streptomycin-resistant <i>Pectobacterium</i> strains in South Korea, and the development of antibiotic alternatives to control soft rot is needed.
Nguyen Trung Dung,Nguyen Van Hiep,Manh B. Nguyen,Vu Dinh Thao,Nguyen Nhat Huy 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.10
Photocatalysis is usually considered as one of the most effective methods for treating non-biodegradable pollutants commonly found in textile wastewater. In this study, the photocatalyst of g-C3N4/MIL-53(Fe) was synthesized by the hydrothermal method and applied for the removal of Rhodamine B (RhB) in water. The photocatalytic material was characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, Brunauer-Emmett-Teller analysis, UV-Vis diffuse reflectance spectroscopy, and X-ray photoelectron spectroscopy. The results showed that the g-C3N4 doped MIL-53(Fe) with 97 wt% of MIL- 53(Fe) works effectively under visible light and the presence of oxidants (Na2S2O8). RhB removal efficiency can be more than 99% with 20mg/L of RhB, 300mg/L of catalyst, 200mg/L of Na2S2O8, and pH 3. In addition, the photocatalytic degradation mechanism of RhB with g-C3N4/MIL-53(Fe) was also proposed, which could be improved and studied for a wide range of applications in textile wastewater treatment.
Correlations between abnormalities of morphological details and DNA fragmentation in human sperm
Nguyen, Hiep Tuyet Thi,Dang, Hong Nhan Thi,Nguyen, Thai Thanh Thi,Nguyen, Trung Van,Dang, Thuan Cong,Nguyen, Quoc Huy Vu,Le, Minh Tam The Korean Society for Reproductive Medicine 2022 Clinical and Experimental Reproductive Medicine Vol.49 No.1
Objective: As the associations of sperm DNA fragmentation with morphology have not been examined in detail, this study aimed to investigate the relationship between abnormalities of morphological details and DNA integrity in human sperm. Methods: In this cross-sectional study, men from infertile couples were enrolled at Hue Center for Reproductive Endocrinology and Infertility, Vietnam. Conventional semen parameters, including morphological details, were analyzed following the World Health Organization 2010 criteria. Sperm DNA fragmentation was evaluated using a sperm chromatin dispersion assay. The relationships and correlations between semen parameters, sperm morphology, and the type of halosperm and the DNA fragmentation index (DFI) were analyzed. Results: Among 130 men in infertile couples, statistically significant differences were not found in the sperm halo type between the normal and abnormal sperm morphology groups. The percentage of round-head spermatozoa was higher in the DFI >15% group (16.98%±12.50%) than in the DFI ≤15% group (13.13% ±8.82%), higher values for amorphous heads were found in the DFI >15% group, and lower values for tapered heads were observed in the DFI ≤15% group; however, these differences were not statistically significant. Small-halo sperm and the DFI were positively correlated with round-head sperm (r=0.243, p=0.005 and r=0.197, p=0.025, respectively). Conclusion: The rate of general sperm morphological abnormalities in semen analysis was not related to sperm DNA integrity. However, round sperm heads were closely associated with sperm DNA fragmentation.
Nguyen Thu Hang,Nguyen Mai Chi,Nguyen Hoang Trung,Thi Y Van Tran,Vu Ngoc Trung,Thu Hang Bui,Duc Trinh Chu,Bui Tung Thanh,Jen Chun-Ping,Quang Loc Do 한국농업기계학회 2024 바이오시스템공학 Vol.49 No.1
Purpose Circulating tumor cell separation and analysis have played a critical role in cancer diagnosis, prognosis, and treatment. In this work, we aim to design and investigate a novel biochip that integrates dielectrophoresis, microfl uidic technology to separate circulating tumor cells from blood cells. To create a dielectrophoresis-induced non-uniform electric fi eld, a facing-electrode design was proposed and utilized, in which a slanted electrode array and a simple rectangular ground electrode are placed parallel on the top and bottom parts of the microfl uidic channel, respectively. This design can reduce the particle position dependence in the microchannel and the complexity of the microfabrication process. Methods The separation process, effi ciency, and optimization of the proposed device were numerically investigated using the fi nite element method. Parametric research was conducted to comprehensively examine the impact of various operating and design factors on the cell movement and trajectories in the microfl uidic device. Results The results indicated the potential of the proposed biochip to ensure cancer cell separation from blood cells with high effi ciency, high purity in a label-free, non-invasive, easy integration, and low-cost manner. Under the optimal conditions, the separation effi ciency reached 92%, 88%, and 96% for human colon cancer cells (HT-29), red blood cells, and white blood cells, respectively. Conclusions In this study, a novel DEP-based microfl uidic chip was proposed to separate HT-29 tumor cells from blood cells and numerically investigated to verify the performance of the biochip design. Our fi ndings could provide a foundation for further theoretical and practical investigations. The proposed system can separate cancer cells from red blood cells and white blood cells as well as off ers numerous advantages, such as compact size, low voltage, high effi ciency, non-invasiveness, and label-free nature. The tumor cell enrichment platform has the potential for application in cancer detection, analysis, and assessment.