Screening and optimization of pretreatments in the preparation of sugarcane bagasse feedstock for biohydrogen production and process optimization

Saratale, Ganesh Dattatray,Saratale, Rijuta Ganesh,Kim, Sang Hyoun,Kumar, Gopalakrishnan Elsevier 2018 International journal of hydrogen energy Vol.43 No.25

<P><B>Abstract</B></P> <P>This work evaluated the effects of individual alkaline, sodium carbonate (Na<SUB>2</SUB>CO<SUB>3</SUB> denoted as; NaC), sodium sulfide (Na<SUB>2</SUB>SO<SUB>3</SUB> denoted as; NaS) and combination of NaC + NaS pretreatment for the saccharification of sugarcane bagasse (SCB). The effects of different pretreatments on chemical composition and structural complexity of SCB in relation with its saccharification were investigated. For enzymatic hydrolysis of pretreated SCB we have utilized the produced crude enzymes by <I>Streptomyces</I> sp. MDS to make the process more cost effective. A enzyme dose of 30 filter paperase (FPU) produced a maximum reducing sugar (RS) 592 mg/g with 80.2% hydrolysis yield from NaC + NaS pretreated SCB under optimized conditions. The resulted enzymatic hydrolysates of each pretreated SCB were applied for hydrogen production using <I>Clostridium beijerinckii</I> KCTC1785. NaC + NaS pretreated SCB hydrolysates exhibited maximum H<SUB>2</SUB> production relative to other pretreatment methods. Effects of temperature, initial pH of culture media and increasing NaC + NaS pretreated SCB enzymatic hydrolysates concentration (2.5–15 g/L) on bioH<SUB>2</SUB> production were investigated. Under the optimized conditions, the cumulative H<SUB>2</SUB> production, H<SUB>2</SUB> production rate, and H<SUB>2</SUB> yield were 1485 mL/L, 61.87 mL/L/h and 1.24 mmol H<SUB>2</SUB>/mol of RS (0.733 mmol H<SUB>2</SUB>/g of SCB), respectively. The efficient conversion of the SCB hydrolysate to H<SUB>2</SUB> without detoxification proves the viability of process for cost-effective hydrogen production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pretreatments of alkaline, sodium carbonate, sodium sulfide and combination demonstrated for sugarcane baggase. </LI> <LI> Several crucial factors towards optimization were carried out. </LI> <LI> Peak 59.2 g/L of reducing sugar (RS) with 80.2% hydrolysis yield from NaC + NaS pretreated SCB. </LI> <LI> Significant hydrogen production and process optimization using SCB hydrolysates studied in detail. </LI> </UL> </P>

Anti-diabetic Potential of Silver Nanoparticles Synthesized with Argyreia nervosa Leaf Extract High Synergistic Antibacterial Activity with Standard Antibiotics Against Foodborne Bacteria

Saratale, Ganesh Dattatraya,Saratale, Rijuta Ganesh,Benelli, Giovanni,Kumar, Gopalakrishnan,Pugazhendhi, Arivalagan,Kim, Dong-Su,Shin, Han-Seung Springer-Verlag 2017 Journal of cluster science Vol.28 No.3

<P>The current investigation highlighted a novel cost-effective green synthesis of silver nanoparticles (AgNPs) using Argyreia nervosa leaves extract (ANE) as a potential reducing and capping agent. Surface plasmon resonance confirmed the formation of AgNPs with maximum absorbance at lambda (max) = 435 nm. FTIR revealed the involvement of biological macromolecules of ANE in the synthesis and stabilization of AgNPs. HRTEM images showed that the size of the spherical AgNPs ranged between 5 and 40 nm with average particle size of about 15 nm. The ANE-AgNPs showed inhibition activity against carbohydrate digestive enzymes alpha-amylase and alpha-glucosidase, with EC50 of 55.5 and 51.7 A mu g/mL, respectively, indicating its antidiabetic potential. The in vitro antioxidant activity of ANE-AgNPs was evaluated in terms of ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radicals scavenging assays with IC50 value of 44.3 and 55.9 A mu g/mL, respectively. The AgNPs displayed strong antibacterial activity against foodborne bacteria with zone of inhibition 16.0 and 12.5 mm for Escherichia coli and Staphylococcus aureus, respectively, and also exhibited strong synergistic antibacterial activity together with standard antibiotics. The biological activity in terms of antioxidant, antidiabetic and antibacterial potential could be useful in various bio-applications such as cosmetics, food, and biomedical industry.</P>

Combined effect of inorganic salts with calcium peroxide pretreatment for kenaf core biomass and their utilization for 2,3-butanediol production

Saratale, Rijuta Ganesh,Shin, Han Seung,Ghodake, Gajanan S.,Kumar, Gopalakrishnan,Oh, Min Kyu,Saratale, Ganesh Dattatraya Elsevier 2018 Bioresource technology Vol.258 No.-

<P><B>Abstract</B></P> <P>This study focuses on development of calcium peroxide (CaO<SUB>2</SUB>) pretreatment that removes major part of lignin but retaining most of sugar components of kenaf core powder (KCP) biomass. In chemical pretreatment, usually higher loss of biomass occurs which was less during this pretreatment strategy. Supplementation of inorganic salts; manganese sulfate (MnSO<SUB>4</SUB>) and cobalt chloride (COCl<SUB>2</SUB>) in CaO<SUB>2</SUB> pretreatment resulted in maximum delignification of KCP relative to individual CaO<SUB>2</SUB> pretreatment. Maximum glucose yield (98%) and hydrolysis yield (80.5%) was achieved after enzymatic hydrolysis (30 FPU/g of KCP) under optimized conditions. Analytical results proved effective lignin removal and significant destruction of KCP with this pretreatment strategy. Finally, utilization of KCP enzymatic hydrolysates by developed strain <I>Klebsiella pneumoniae</I> KMK05 resulted in maximum 2,3-butanediol (BDO) production (10.42 g/L) and BDO titer (0.385 g/g of sugar). BDO titer achieved with KCP derived sugars were found comparable with the mixture of standard sugars which is notable.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Developed CaO<SUB>2</SUB> pretreatment & optimized various operational parameters for KCP biomass. </LI> <LI> Addition of inorganic salts improved delignification, glucose yield and total sugar recovery. </LI> <LI> Maximum BDO production (10.42 g/L) was achieved using <I>Klebsiella pneumoniae</I> KMK05. </LI> <LI> This novel pretreatment strategy abstained loss of sugar components of biomass. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Electrochemical Oxidation of Phenol for Wastewater Treatment Using Ti/PbO2 Electrode

Saratale, Rijuta Ganesh,Hwang, Kyoung-Jin,Song, Ji-Young,Saratale, Ganesh Dattatray,Kim, Dong-Su American Society of Civil Engineers 2016 Journal of environmental engineering Vol.142 No.2

<P>The electrochemical oxidation of phenol was studied using a Ti/PbO2 electrode prepared by the electrodeposition method with PbO2 coated on Ti. The structural and morphological activity of Ti/PbO2 was analyzed by scanning electron microscopy (SEM) and X-ray diffraction (XRD), and intermediates formed after degradation of phenol were quantitatively assessed by high-pressure liquid chromatography (HPLC). Optimization of various parameters such as current density, initial phenol concentration, initial solution pH, and different temperature and dose of Fe2+ on electrochemical degradation of phenol using Ti/PbO2 were investigated. Complete removal of phenol (250mgL-1) was observed at 50 degrees C, potential difference (5V), and at pH 2. Experimental results showed that the phenol removal rate increased with increasing current intensity along with significant reduction in total organic carbon (TOC). Fundamental kinetic data obtained for the degradation of phenol by Ti/PbO2 was found to follow in accordance with the zero-order kinetics with respect to the phenol concentration. This paper is expected to be useful for the development of electrochemical process using Ti/PbO2 for the degradation of phenol containing wastewater. (C) 2015 American Society of Civil Engineers.</P>

Wind flow modification by a jet roof for mitigation of snow cornice formation

Ganesh Kumar,Ajay Gairola,Aditya Vaid 한국풍공학회 2021 Wind and Structures, An International Journal (WAS Vol.32 No.2

The snow cornice mass on the formation zone had triggered avalanches which led to the loss of human life and property. Snow cornice is formed due to flow separation on the leeward side. Effect of lee slope is more prominent in the formation of snow cornices as compared to the windward slope. The analysis of wind flow pattern has been carried out to evaluate the performance of a jet roof. Computational Fluid Dynamics (CFD) analysis of wind flow over a 2D hill model was carried out using RNG based k- turbulence models available in ANSYS Fluent. Effect of varying leeward hill slope (1:2 to 1:6) on flow separation for the given windward slope was observed and a critical slope of 1:4 was found at which the separation zone ceased to exist. The modification of wind flow over a hill due to the installation of jet roof was simulated. It was observed that jet roof had significantly modified the wind flow pattern around hill ridgeline and ultimately snow cornice formation had mitigated. The results of the wind flow pattern were validated with the wind data collected at the experimental site, Banihal Top (Jammu and Kashmir, India). The wind flow simulation over the hill and mitigation of cornice formation by the jet roof has been explained in the present paper.

Spectral Analysis of Rectangular, Hanning, Hamming and Kaiser Window for Digital Fir Filter

Ganesh Gautam,Surendra Shrestha,Seongsoo Cho 한국인터넷방송통신학회 2015 Journal of Advanced Smart Convergence Vol.4 No.2

Digital filters are extensively used in the world of communication. In order to design a digital finite impulse response (FIR) filter that satisfies all the required conditions is challenging. In this paper, design techniques of digital low pass FIR filters using Rectangular window method, Hamming window, Hanning window, and Optimal Parks McClellan method are presented. The stability, number of components required and filter coefficients are demonstrated for different design techniques. It is demonstrated that filter design using hamming window is comparatively better than rectangular and hanning window though the components required for all of the windowing technique are same, hamming shows higher stability. The stability is shown with the help of magnitude and phase spectrum of each window. Simulation is carried out using MATLAB and comparisons are made entirely based on the output of the simulation.

ESTIMATION OF MELOXICAM IN HUMAN PLASMA BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY (LC-MS/MS)

Ganesh, M.,Hemalatha, P.,Peng, M.M.,Sakthimanigandan, K.,Jang, H.T. Taylor Francis 2013 JOURNAL OF LIQUID CHROMATOGRAPHY AND RELATED TECHN Vol.36 No.7

Growth, microstructure, structural and optical properties of PVP-capped CdS nanoflowers for efficient photocatalytic activity of Rhodamine B

Ganesh, R. Sankar,Sharma, Sanjeev K.,Durgadevi, E.,Navaneethan, M.,Ponnusamy, S.,Muthamizhchelvan, C.,Hayakawa, Y.,Kim, Deuk Young Elsevier 2017 Materials research bulletin Vol.94 No.-

<P><B>Abstract</B></P> <P>Size and structure tunability of polyvinyl pyrolidone (PVP) capped cadmium sulfide (PVP-capped CdS) flowers were synthesized by controlling the reaction time. XRD pattern indicated a phase transition from the cubic zinc blend to the hexagonal phase of CdS powder as the reaction time increased. As per the microstructure analysis with respect to the reaction time, the spherical nanoparticles were transformed in to the flower like morphology consisting of sword like nanorods and confirmed by the bright field images of TEM, while, HR-TEM images showed the uniform lattice spacing. UV-spectra exhibited a red shift due to the excitations of CdS nanoparticles. The near band edge (NBE) peak of PVP-capped CdS was completely diminished which indicated to sulphur vacancies on the surface of flowers. The photocatalytic activity of PVP-capped CdS flowers was detected the fast degradation of Rhodamine B (RhB) dye (95% for 120min) by irradiation of visible light.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Synthesis of un-capped and PVP-capped CdS microstructures by Hydrothermal. </LI> <LI> Growth of both the flower and dendrite microstructures controlled by the growth time. </LI> <LI> Phase transition of CdS from cubic zinc blend to hexagonal by the growth time. </LI> <LI> CdS flowers enhanced the photocatalytic performance of RhB under visible light. </LI> <LI> PVP-capped CdS showed the highest degradation of 95% of RhB within 120min. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Tuberculosis and Respiratory Diseases : Idiopathic Pulmonary Fibrosis (IPF), A Distinct Subgroup of Idiopathic Interstitial Pneumonia (IIP): Evolving Definition And Current Concepts In Management

( Ganesh Raghu ) 대한결핵 및 호흡기학회 2001 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.93 No.0

Quality of Life among Breast Cancer Patients In Malaysia

Ganesh, Sri,Lye, Munn-Sann,Lau, Fen Nee Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Background: Among the factors reported to determine the quality of life of breast cancer patients are socio-demographic background, clinical stage, type of treatment received, and the duration since diagnosis. Objective: The objective of this study was to determine the quality of life (QOL) scores among breast cancer patients at a Malaysian public hospital. Materials and Methods: This cross-sectional study of breast cancer patients was conducted between March to June 2013. QOL scores were determined using the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ-C30) and its breast cancer supplementary measure (QLQ-BR23). Both the QLQ-C30 and QLQ-BR23 assess items from functional and symptom scales. The QLQ-C30 in addition also measures the Global Health Status (GHS). Systematic random sampling was used to recruit patients. Results: 223 breast cancer patients were recruited with a response rate of 92.1%. The mean age of the patients was 52.4 years (95% CI = 51.0, 53.7, SD=10.3). Majority of respondents are Malays (60.5%), followed by Chinese (19.3%), Indians (18.4%), and others (1.8%). More than 50% of respondents are at stage III and stage IV of malignancy. The mean Global Health Status was 65.7 (SD = 21.4). From the QLQ-C30, the mean score in the functioning scale was highest for 'cognitive functioning' (84.1, SD=18.0), while the mean score in the symptom scale was highest for 'financial difficulties' (40.1, SD=31.6). From the QLQ-BR23, the mean score for functioning scale was highest for 'body image' (80.0, SD=24.6) while the mean score in the symptom scale was highest for 'upset by hair loss' (36.2, SD=29.4). Two significant predictors for Global Health Status were age and employment. The predictors explained 10.6% of the variation of global health status ($R^2=0.106$). Conclusions: Age and employment were found to be significant predictors for Global Health Status (GHS). The Quality of Life among breast cancer patients reflected by the GHS improves as age and employment increases.