Clinical-pathologic profile of head and neck rhabdomyosarcoma in children: a systematic review

Ludimila Lem es Moura(Ludimila Lemes Moura ),Beatriz Della Terra Mouco Garrido(Beatriz Della Terra Mouco Garrido ),Nelson Leonel Del Hierro Polanco(Nelson Leonel Del Hierro Polanco ),Mattheus Augusto 대한구강악안면외과학회 2023 대한구강악안면외과학회지 Vol.49 No.2

This systematic review aimed to analyze the clinicopathological profile and relevant prognostic factors of head and neck rhabdomyosarcoma in pediatric patients. The search was carried out in the electronic search portals PubMed, Lilacs, Embase, Scopus, and Web of Science. The search yielded studies that were then analyzed regarding study topic, data extraction, and risk of bias using the STROBE (Strengthening the Reporting of Observational Studies) guidelines. Finally, three studies were included for qualitative analysis. Most of the cases involved embryonic and alveolar rhabdomyosarcoma. Expression of MYOD1 was highly correlated with diagnosis of spindle cell/sclerosing rhabdomyosarcoma, which appears to have a poor prognosis in children. Furthermore, tumor size <5 cm and absence of metastasis accompanied by complete resection and administration of adjuvant therapies such as chemotherapy and radiotherapy favored a better prognosis.

Endoscopic Ultrasound-Guided Fine Needle Aspiration and Endoscopic Retrograde Cholangiopancreatography-Based Tissue Sampling in Suspected Malignant Biliary Strictures: A Meta-Analysis of Same-Session Procedures

Diogo Turiani Hourneax de Moura,Marvin Ryou,Eduardo Guimarães Hourneaux De Moura,Igor Braga Ribeiro,Wanderlei Marques Bernardo,Christopher C. Thompson 대한소화기내시경학회 2020 Clinical Endoscopy Vol.53 No.4

Background/Aims: The diagnosis of biliary strictures can be challenging. There are no systematic reviews studying same-sessionendoscopic retrograde cholangiopancreatography (ERCP)-based tissue sampling and endoscopic ultrasound-guided fine needleaspiration (EUS-FNA) for the diagnosis of biliary strictures. Methods: A systematic review was conducted on studies analyzing same-session EUS and ERCP for tissue diagnosis of suspectedmalignant biliary strictures. The primary outcome was the accuracy of each method individually compared to the two methodscombined. The secondary outcome was the accuracy of each method in pancreatic and biliary etiologies. In the meta-analysis, we usedForest plots, summary receiver operating characteristic curves, and estimates of the area under the curve for intention-to-treat analysis. Results: Of the 12,132 articles identified, six were included, resulting in a total of 497 patients analyzed. The sensitivity, specificity,positive likelihood ratio, negative likelihood ratio, and accuracy of the association between the two methods were: 86%, 98%, 12.50,0.17, and 96.5%, respectively. For the individual analysis, the sensitivity, specificity and accuracy of EUS-FNA were 76%, 100%, and94.5%, respectively; for ERCP-based tissue sampling, the sensitivity, specificity, and accuracy were 58%, 98%, and 78.1%, respectively. For pancreatic lesions, EUS-FNA was superior to ERCP-based tissue sampling. However, for biliary lesions, both methods had similarsensitivities. Conclusions: Same-session EUS-FNA and ERCP-based tissue sampling is superior to either method alone in the diagnosis of suspectedmalignant biliary strictures. Considering these results, combination sampling should be performed when possible.

The Severity of Cleft Lip and Palate and Postsurgical Outcome in Non-syndromic Complete Unilateral Cleft Lip and Palate: A Pilot Study Based on Surgeon’s Perception

Pollyana Marques de Moura(Pollyana Marques de Moura),James Seaward(Seaward James),Alex Kane(Alex Kane),Yong Jong Park(Yong Jong Park) 대한구순구개열학회 2023 대한구순구개열학회지 Vol.26 No.1

Objective: The aim of this study was to assess the pre-operative and post-operative aesthetic outcome of patients following primary lip repair to investigate the association of the severity of the cleft deformity after NAM therapy and final surgical outcome. Materials and Methods: To assess the correlation between severity of the cleft and the primary surgery outcome, four pediatric craniofacial surgeons from the affiliated teaching hospital were asked to independently rate 156 randomized sets of photographs from 39 case of non-syndromic patients with complete unilateral cleft lip and palate who received NAM treatment.by mean of visual analogue scale. The photographs were acquired in four different time points: (T1) Pre NAM therapy, (T2) Post NAM therapy, (T3) Immediate post-operative, (T4) Late post-operative. Results: The mean of VAS score for lip and nose improved significantly after NAM therapy. There was significant correlation between NAM treatment (T2) outcome and immediate post-operative outcome (T3) for nose. There was no significant correlation between NAM treatment outcome (T2) and immediate postoperative outcome (T3) or late postoperative outcome for lip. Conclusion: Cleft deformity improved significantly after NAM therapy and this improvement led to better nasal esthetics immediate post operatively.

Role of Cardiac Septal Occluders in the Treatment of Gastrointestinal Fistulas: A Systematic Review

Diogo Turiani Hourneaux De Moura,Alberto Baptista,Pichamol Jirapinyo,Eduardo Guimarães Hourneaux De Moura,Christopher Thompson 대한소화기내시경학회 2020 Clinical Endoscopy Vol.53 No.1

Treating gastrointestinal (GI) fistulas endoscopically is challenging owing to an established epithelial tract. The variety of endoscopicapproaches is transforming endoscopy into a first-line therapy. However, many sessions are often required, with variable success rates. Owing to these limitations, the off-label use of cardiac septal occluders (CSOs) has been reported. We searched for articles related to CSOs in the MEDLINE, EMBASE, Cochrane Library, and LILACS databases and gray literature. Theprimary outcomes included technical success, clinical success, and safety of CSOs in GI fistula management. A total of 25,574 records were identified, and 19 studies ultimately satisfied the inclusion criteria. Technical success was achieved inall cases. Of the 22 fistulas, 77.27% had successful closure, with a mean follow-up period of 32.02 weeks. The adverse event rate was22.72%, with no associated mortality. Univariable and multivariable regression analyses showed no significant difference in the successof closure and adverse events in relation to several variables among the subgroups. The use of CSOs appeared to be technically feasible, effective, and safe in the treatment of GI fistulas. The satisfactory results derived fromthis sparse literature suggest that it can be an option in the management of GI fistulas.

What does not kill it does not always make it stronger: High temperatures in pyriproxyfen treatments produce Aedes aegypti adults with reduced longevity and smaller females

Moura Lidia,de Nadai Barbara Lepretti,Corbi Juliano J. 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2

Aedes aegypti control in Brazil comprises integrated actions, in which larvicide application is a supplementary measure. Despite the importance of analyzing the effects of temperature on the efficiency of larvicides to control mosquito populations, there is still a lack of information regarding the sublethal effect of larvicides. We hypothesized that mosquitoes which survived pyriproxyfen exposure, during their immature development, have small body sizes and live less than mosquitoes that were not exposed to this larvicide. We investigated the sublethal effects of five different pyriproxyfen concentrations (0.0001; 0.001; 0.01; 0.1 and 1 mg.L −1 ), under three different temperatures (20, 25 and 30 °C). As we increased the larvicide concentration, less larvae survived and developed into adults. However, at 30 °C the survival was higher than at 25 °C and 20 °C comparing the concentrations of 0.001 mg.L −1 and 0.01 mg.L −1 (10% and 16% high, respectively). Mosquito survivors to pyriproxyfen exposure, in all thermal conditions, had shorter adult life spans than mosquitoes not exposed to pyriproxyfen during the larval stage. The females exposed at 30 °C showed smaller wings than females from experiments at 20 °C. These findings provide evidence that the biological parameters of the adult lifespan and wing centroid size are impaired due to larval exposure to pyriproxyfen, even at high temperatures. Reduced longevity and small wing size are fitness costs to survive this larvicide exposure. These findings provide support to assess resistance development to larvicides through future generations and contribute to the discussion on improving the rational application of larvicides.

Effects of energy-protein supplementation frequency on performance of primiparous grazing beef cows during pre and postpartum

Moura Felipe Henrique de,Costa Thaís Correia,Trece Aline Souza,Melo Luciano Prímola de,Manso Marcos Rocha,Paulino Mário Fonseca,Rennó Luciana Navajas,Fonseca Mozart Alves,Detmann Edenio,Gionbelli Mate 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.9

Objective: Twenty-four pregnant Nellore primiparous grazing cows were used to evaluate the effects of energy-protein supplementation and supplementation frequency during pre (105 d before calving) and postpartum (105 d after calving) on performance and metabolic characteristics. Methods: Experimental treatments consisted of a control (no supplementation), daily supplementation (1.5 kg/d of concentrate/animal) and infrequent supplementation (4.5 kg of concentrate/ animal every three days). During the pre and postpartum periods, concentrations of blood metabolites and animal performance were evaluated. Ureagenesis and energy metabolism markers were evaluated at prepartum period. Results: Supplementation frequency did not alter (p>0.10) body weight (BW), average daily gain (ADG), and carcass traits during pre and postpartum. The BW (p = 0.079), adjusted BW at day of parturition (p = 0.078), and ADG (p = 0.074) were greater for supplemented cows during the prepartum. The body condition score (BCS; p = 0.251), and carcass traits (p>0.10) were not affected by supplementation during prepartum. On postpartum, supplementation did not affect animal performance and carcass traits (p>0.10). The dry mater intake was not affected (p>0.10) by supplementation and supplementation frequency throughout the experimental period. Daily supplemented animals had greater (p<0.001) glucose levels than animals supplemented every three days. Supplementation and supplementation frequency did not alter (p>0.10) the levels of blood metabolites, neither the abundance of ureagenesis nor energy metabolism markers. Conclusion: In summary, our data show that the reduction of supplementation frequency does not cause negative impacts on performance and metabolic characteristics of primiparous grazing cows during the prepartum.

Better eggs today than psocids tomorrow: Aedes aegypti (Diptera: Culicidae) eggs exposed to Liposcelis sp. (Psocodea: Liposcelididae) has reduced hatching rates

Moura Lidia,de Nadai Barbara Lepretti,Oyamaguti Maria Eduarda Yumi,Corbi Juliano J. 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.4

For different research purposes, there is a need to mass rear mosquitoes, such as Aedes aegypti, under laboratory conditions. The rearing process begins with egg production followed by egg storage in a dry environment, inside containers. Stored eggs are susceptible to environmental threats when storage conditions are suboptimal. Some terrestrial insects can invade this environment and attack stored eggs. In this brief report, we assessed whether Ae. aegypti eggs exposed to Liposcelis sp. individuals had reduced hatching and immature development rates. We exposed 100 eggs in different treatment conditions (fixed in porous paper and loosed) to 30 Liposcelis sp. in dividuals for ten days and then we induced hatching. We observed a hatching rate of 99% reduced for those eggs adhered to porous paper and loosed eggs showed a hatching rate of 45% decreased for those eggs exposed to Liposcelis sp. The remaining larvae took longer to develop into pupae as well, showing a four-day delay on average to the final metamorphosis of the aquatic stage. These results reinforce the need to frequently monitor egg storage conditions to maintain laboratory colonies stable and free from pests that can interfere with mosquito life-history traits.

Modeling lactose hydrolysis for efficiency and selectivity: Toward the preservation of sialyloligosaccharides in bovine colostrum whey permeate

de Moura Bell, J.M.L.N.,Aquino, L.F.M.C.,Liu, Y.,Cohen, J.L.,Lee, H.,de Melo Silva, V.L.,Rodrigues, M.I.,Barile, D. American Dairy Science Association 2016 Journal of dairy science Vol.99 No.8

<P>Enzymatic hydrolysis of lactose has been shown to improve the efficiency and selectivity of membrane-based separations toward the recovery of bioactive oligosaccharides. Achieving maximum lactose hydrolysis requires intrinsic process optimization for each specific substrate, but the effects of those processing conditions on the target oligosaccharides are not well understood. Response surface methodology was used to investigate the effects of pH (3.25-8.25), temperature (35-55 degrees C), reaction time (6 to 58 min), and amount of enzyme (0.05-0.25%) on the efficiency of lactose hydrolysis by beta-galactosidase and on the preservation of biologically important sialyloligosaccharides (3'-siallylactose, 6-siallylactose, and 6'-sialyl-N-acetyllactosamine) naturally present in bovine colostrum whey permeate. A central composite rotatable design was used. In general, beta-galactosidase activity was favored at pH values ranging from 3.25 to 5.75, with other operational parameters having a less pronounced effect. A pH of 4.5 allowed for the use of a shorter reaction time (19 min), lower temperature (40 degrees C), and reduced amount of enzyme (0.1%), but complete hydrolysis at a higher pH (5.75) required greater values for these operational parameters. The total amount of sialyloligosaccharides was not significantly altered by the reaction parameters evaluated, suggesting specificity of beta-galactosidase from Aspergillus oryzae toward lactose as well as the stability of the oligosaccharides at pH, temperature, and reaction time evaluated.</P>

An In Vitro and In Vivo Study of the α-Amylase Activity of Phaseolamin

Neire Moura de Gouveia,Fernanda Vieira Alves,Fabiana Barcelos Furtado,Danielli Luana Scherer,Antonio Vicente Mundim,Foued Salmen Espindola 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.8

We evaluated the polypeptide profiles, inhibition of human salivary a-amylase activity, and hemagglutination properties of a commercial phaseolamin sample. We also performed an in vivo assay to investigate the effects of a commercial phaseolamin treatment (100, 500, or 1500 mg/kg) over 20 days on the glycemia, body weight, and serum biochemical parameters (total cholesterol, triglycerides, alanine aminotransferase, and aspartate aminotransferase) of nondiabetic and streptozotocin-induced diabetic rats. The in vitro evaluation showed defined protein profiles, low hemagglutination activity, and high a-amylase inhibition. None of the experimental groups treated with phaseolamin or acarbose showed decreases in body weight. Our data demonstrate that phaseolamin inhibits amylase activity in vitro, reduces blood glucose levels, decreases or attenuates some of the renal and hepatic effects of diabetes in streptozotocin-induced rats, and could therefore have therapeutic potential in the treatment or prevention of the complications of diabetes.

Evaluation of microbiological, cellular and risk factors associated with subclinical mastitis in female buffaloes

de Oliveira Moura, Emmanuella,do Nascimento Rangel, Adriano Henrique,de Melo, Maria Celeste Nunes,Borba, Luiz Henrique Fernandes,de Lima, Dorgival Morais Junior,Novaes, Luciano Patto,Urbano, Stela Ant Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.9

Objective: This study aimed to evaluate the microbiological and cellular milk profile for the diagnosis of subclinical mastitis in female buffaloes and to assess risk factors for predisposition of the disease. Methods: Analyses were carried out by standard plate count (SPC), identification of species and antibiotic resistance, somatic cell count (SCC), electrical electrical conductivity of milk (ECM), and lactoferrin content in milk. Teat cups were swabbed to evaluate risk factors, observing hyperkeratosis, milking vacuum pressure and cleanliness of the site. Hence, 30 female buffaloes were randomly selected (15 from a group in early lactation and 15 in late lactation). Results: The most common bacteria in the microbiological examination were Staphylococcus spp., Streptococcus spp. and Corynebacterium sp. In the antibiotic sensitivity test, 10 (58.82%) of the 17 antibiotics tested were sensitive to all isolates, and resistant bacteria were Streptococcus uberis, Streptococcus dysgalactiae, Streptococcus haemolyticus, and Escherichia coli. It was observed that positive samples in the microbiological examination showed total bacterial count between $9.10{\times}10^3$ to $6.94{\times}10^6$ colony forming units/mL, SCC between 42,000 to 4,320,000 cells/mL and ECM ranging from 1.85 to 7.40 mS/cm. It was also found that the teat cups had high microbial counts indicating poor hygiene, and even faults in the cleanliness of the animals' waiting room were observed. It is concluded that values of SCC above 537,000 cells/mL and ECM above 3.0 mS/mL are indications of mammary gland infection for this herd; however, the association of these values with a microbiological analysis is necessary to more accurately evaluate the health status of mammary glands with subclinical mastitis. Conclusion: Through phenotypic characterization of bacteria involved in the samples, the genera Staphylococcus spp., Streptococcus spp., and Corynebacterimum bovis were the most prevalent in this study. Faults in environment and equipment hygienization are factors that are directly associated with mastitis.