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최문정(MoonJung Choi),황재관(Jae-Kwan Hwang) 한국식품영양과학회 1998 한국식품영양과학회지 Vol.27 No.5
단백질과 다당류는 식품의 주요 구성성분이며, 일반적으로 이들 두 거대분자들의 혼합용액은 열역학적 비혼합성으로 인해서 상분리가 일어난다. 상분리는 평형상도해로 나타낼 수 있는데, 상도해는 binodal curve, critical point, phase separation threshold, tie-line 그리고 rectilinear diameter를 포함한다. 단백질과 다당류 혼합용액의 상분리는 pH, 온도, 염농도, 그리고 거대분자의 분자량과 분자구조에 영향을 받는다. 단백질과 다당류 혼합용액의 상분리현상을 기초로 하여 단백질 용액을 농축할 수 있는 무막삼투현상이 고안되었다. 또한 단백질-다당류 혼합물은 각각의 생고분자가 가지고 있는 겔특성을 서로 보완하여 가소성의 조직감과 입안촉감을 줄 수 있으므로 효과적인 지방대체제로 사용될 수 있으리라 본다. Proteins and polysaccharides are major food macromolecules. Generally, the mixture of these macromolecules can be separated into two phases because of their thermodynamic incompatibility. Phase separ-ation is explained by equilibrium phase diagram, which comprises binodal curve, critical point, phase separation threshold, tie-line and rectilinear diameter. Phase separation of protein-polysaccharide solution is affected by pH, temperature, ionic strength, molecular weight, molecular structure, etc. Membraneless osmosis has been developed to concentrate protein solutions, using the phase diagram constituted by proteins and polysaccharides. Protein-polysaccharide mixtures are very promising fat mimetics because solution of mixtures forms water-continuous system with two phase-separated gels, which give plastic texture and a fatty mouthfeel.
Antibacterial Activity of Lysozyme - Galactomannan Conjugate against Escherichia coli
Jae-Kwan Hwang,Hyun-Jin Kim,MoonJung Choi,Hae-Hun Shin,Yu-Ryang Pyun 한국식품영양과학회 1998 Preventive Nutrition and Food Science Vol.3 No.4
Lysozyme was covalently conjugated with galactomannan through a amino-carbonyl reaction between the lysine ε -amino groups of lysozyme and the reducing ends of galactomannan at a relative humidity of 79% and 60℃. The resulting lysozyme-galactomannan conjugate (LGC) was investigated for its antibacterial activity against Escherichia coli. Lysozyme alone did not exhibit antibacterial activity against E. coli. In contrast, significant bactericidal effect was observed for LGC, depending on the reaction temperature. The degree of conjugation between lysozyme and galactomannan was dependent on the incubation time, which affected the antibacterial efficiency against E. coli. This study demonstrated that the amino-carbonyl reaction between lysozyme and galactomannan could be a potential tool to modify lysozyme toward broadening its antibacterial spectrum to Gram-negative bacteria.
강경미,최승홍,Moonjung Hwang,유노을,윤태진,김지훈,손철호 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.4
Objective: The purpose of this study was to investigate the time-dependent effects of contrast medium on multi-dynamic, multi-echo (MDME) sequence in patients with brain metastases. Materials and Methods: This study included 7 patients with 15 brain metastases who underwent magnetic resonance (MR) examination which included MDME sequences at 1 minute, 10 minutes and 20 minutes after contrast injection. Two volumes of interests, covering an entire tumor (whole tumor) and the enhancing portion of the tumor, were derived from postcontrast synthetic T1-weighted images. Statistical comparisons were performed for three different time delays for histogram parameters of the longitudinal relaxation rate (R1) and the transverse relaxation rate (R2), and lesion volumes. Results: The mean and the median of R1 and the mean of R2 in both the whole tumor and the inner enhancing portion were larger on the 10 minutes delayed images than on the 1 minute or 20 minutes delayed images (mean of R1 in the whole tumor on the 1 minute, 10 minutes, and 20 minutes delayed images: 1.26 ms, 1.39 ms, and 1.37 ms; mean of R1 in the inner enhancing portion: 1.43 ms, 1.53 ms and 1.44 ms; all p < 0.017). The volumes of the whole tumor and the inner enhancing portion were significantly larger in the 10 minutes and 20 minutes delayed images than on the 1 minute delayed images (all p < 0.017). Conclusion: Magnetic resonance relaxation times and the volumes of the whole tumor and the inner enhancing portion were measured larger on the 10 minutes or 20 minutes delayed images than on the 1 minute delayed images. The MDME sequence immediately after contrast injection cannot fully reflect the effects of gadolinium-based contrast agent leakage in the tissue.
황재관(Jae-Kwan Hwang),홍석인(Seok-In Hong),김종태(Chong-Tai Kim),최문정(MoonJung Choi),김윤지(Yun-Ji Kim) 한국식품영양과학회 1998 한국식품영양과학회지 Vol.27 No.3
생미역 및 건미역에 0.1M Na₂CO₃를 가하여 미역내에 분포하는 알긴산을 추출하여 미역 페이스트를 제조한 후, 이를 첨가한 육제품 패티의 관능특성과 물성을 측정하였다. 전체적으로 미역 페이스트의 첨가에 의해 조리한 패티의 맛, 색상, 풍미, 조직감, 다즙성 등 각종 관능특성이 향상되는 현상을 보였다. 경도는 미역 페이스트의 첨가에 의해 크게 감소하여 부드러운 조직감을 제공하였다. 또한, 미역 페이스트의 첨가에 의한 보수력의 증가로 패티의 조리에 의한 무게손실율이 감소하였다. Sea mustard paste was prepared by treating wet and dried sea mustard with 0.1M Na₂CO₃, which mainly aimed to extract alginate from the cell wall of sea mustard. The pastes were added to beef, chicken and pork to manufacture the meat patties. The effects of adding the paste were investigated in terms of sensory properties, texture(hardness) and weight changes after cooking. The sensory attributes such as taste, color, texture and juiciness were generally enhanced by adding the paste. The hardness of cooked patties was significantly decreased, so the paste provided softer texture. The addition of paste also resulted in decreasing the weight loss of meat patties after cooking.
Yoo Roh-Eul,최승홍,Youn Sung-Won,Hwang Moonjung,Kim Eunkyung,Oh Byung-Mo,Lee Ji Ye,Hwang Inpyeong,Kang Koung Mi,Yun Tae Jin,Kim Ji-hoon,Sohn Chul-Ho 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.2
Objective: This study aimed to explore the myelin volume change in patients with mild traumatic brain injury (mTBI) with post-concussion syndrome (PCS) using a multidynamic multiecho (MDME) sequence and automatic whole-brain segmentation. Materials and Methods: Forty-one consecutive mTBI patients with PCS and 29 controls, who had undergone MRI including the MDME sequence between October 2016 and April 2018, were included. Myelin volume fraction (MVF) maps were derived from the MDME sequence. After three dimensional T1-based brain segmentation, the average MVF was analyzed at the bilateral cerebral white matter (WM), bilateral cerebral gray matter (GM), corpus callosum, and brainstem. The Mann–Whitney U-test was performed to compare MVF and myelin volume between patients with mTBI and controls. Myelin volume was correlated with neuropsychological test scores using the Spearman rank correlation test. Results: The average MVF at the bilateral cerebral WM was lower in mTBI patients with PCS (median [interquartile range], 25.2% [22.6%–26.4%]) than that in controls (26.8% [25.6%–27.8%]) (p = 0.004). The region-of-interest myelin volume was lower in mTBI patients with PCS than that in controls at the corpus callosum (1.87 cm3 [1.70–2.05 cm3] vs. 2.21 cm3 [1.86– 3.46 cm3]; p = 0.003) and brainstem (9.98 cm3 [9.45–11.00 cm3] vs. 11.05 cm3 [10.10–11.53 cm3]; p = 0.015). The total myelin volume was lower in mTBI patients with PCS than that in controls at the corpus callosum (0.45 cm3 [0.39–0.48 cm3] vs. 0.48 cm3 [0.45–0.54 cm3]; p = 0.004) and brainstem (1.45 cm3 [1.28–1.59 cm3] vs. 1.54 cm3 [1.42–1.67 cm3]; p = 0.042). No significant correlation was observed between myelin volume parameters and neuropsychological test scores, except for the total myelin volume at the bilateral cerebral WM and verbal learning test (delayed recall) (r = 0.425; p = 0.048). Conclusion: MVF quantified from the MDME sequence was decreased at the bilateral cerebral WM in mTBI patients with PCS. The total myelin volumes at the corpus callosum and brainstem were decreased in mTBI patients with PCS due to atrophic changes.