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Park, Minjeong,Jeon, Yeji,Jang, Ho Hee,Ro, Hyun-Su,Park, Woojun,Madsen, Eugene L.,Jeon, Che Ok American Society for Microbiology 2007 Applied and environmental microbiology Vol.73 No.16
<B>ABSTRACT</B><P>Prior research revealed that <I>Polaromonas naphthalenivorans</I> CJ2 carries and expresses genes encoding the gentisate metabolic pathway for naphthalene. These metabolic genes are split into two clusters, comprising <I>nagRAaGHAbAcAdBFCQEDJI</I>′-<I>orf1</I>-<I>tnpA</I> and <I>nagR2</I>-<I>orf2I</I>″<I>KL</I> (C. O. Jeon, M. Park, H. Ro, W. Park, and E. L. Madsen, Appl. Environ. Microbiol. 72:1086-1095, 2006). BLAST homology searches of sequences in GenBank indicated that the <I>orf2</I> gene from the small cluster likely encoded a salicylate 5-hydroxylase, presumed to catalyze the conversion of salicylate into gentisate. Here, we report physiological and genetic evidence that <I>orf2</I> does not encode salicylate 5-hydroxylase. Instead, we have found that <I>orf2</I> encodes 3-hydroxybenzoate 6-hydroxylase, the enzyme which catalyzes the NADH-dependent conversion of 3-hydroxybenzoate into gentisate. Accordingly, we have renamed <I>orf2 nagX</I>. After expression in <I>Escherichia coli</I>, the NagX enzyme had an approximate molecular mass of 43 kDa, as estimated by gel filtration, and was probably a monomeric protein. The enzyme was able to convert 3-hydroxybenzoate into gentisate without salicylate 5-hydroxylase activity. Like other 3-hydroxybenzoate 6-hydroxylases, NagX utilized both NADH and NADPH as electron donors and exhibited a yellowish color, indicative of a bound flavin adenine dinucleotide. An engineered mutant of <I>P. naphthalenivorans</I> CJ2 defective in <I>nagX</I> failed to grow on 3-hydroxybenzoate but grew normally on naphthalene. These results indicate that the previously described small catabolic cluster in strain CJ2 may be multifunctional and is essential for the degradation of 3-hydroxybenzoate. Because <I>nagX</I> and an adjacent MarR-type regulatory gene are both closely related to homologues in <I>Azoarcus</I> species, this study raises questions about horizontal gene transfer events that contribute to operon evolution.</P>
Implementing curriculum integration: The experiences of Korean elementary teachers
Minjeong Park 서울대학교 교육연구소 2008 Asia Pacific Education Review Vol.9 No.3
This study examines 1) how Korean elementary teachers implement curriculum integration in their teaching practice and 2) what Korean elementary teachers experience in implementing curriculum integration. Several issues evolved from the analysis of three teachers' experiences: firstly, the teachers' lack of theoretical frameworks for curriculum integration, secondly, the teachers' pragmatic approach to curriculum integration, and thirdly, the limitations on implementing Curriculum integration. In order for Curriculum integration to be properly implemented and sustained, teachers' roles in and understanding of the curricula are crucial. Thus, the implications of the findings are discussed in terms of teacher preparation and training.
Effect of dentin treatment on proliferation and differentiation of human dental pulp stem cells
Park, Minjeong,Pang, Nan-Sim,Jung, Il-Young The Korean Academy of Conservative Dentistry 2015 Restorative Dentistry & Endodontics Vol.40 No.4
Objectives: Sodium hypochlorite (NaOCl) is an excellent bactericidal agent, but it is detrimental to stem cell survival, whereas intracanal medicaments such as calcium hydroxide ($Ca[OH]_2$) promote the survival and proliferation of stem cells. This study evaluated the effect of sequential NaOCl and $Ca(OH)_2$ application on the attachment and differentiation of dental pulp stem cells (DPSCs). Materials and Methods: DPSCs were obtained from human third molars. All dentin specimens were treated with 5.25% NaOCl for 30 min. DPSCs were seeded on the dentin specimens and processed with additional 1 mg/mL $Ca(OH)_2$, 17% ethylenediaminetetraacetic acid (EDTA) treatment, file instrumentation, or a combination of these methods. After 7 day of culture, we examined DPSC morphology using scanning electron microscopy and determined the cell survival rate with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. We measured cell adhesion gene expression levels after 4 day of culture and odontogenic differentiation gene expression levels after 4 wk using quantitative real-time polymerase chain reaction. Results: DPSCs did not attach to the dentin in the NaOCl-treated group. The gene expression levels of fibronectin-1 and secreted phosphoprotein-1 gene in both the $Ca(OH)_2$- and the EDTA-treated groups were significantly higher than those in the other groups. All $Ca(OH)_2$-treated groups showed higher expression levels of dentin matrix protein-1 than that of the control. The dentin sialophosphoprotein level was significantly higher in the groups treated with both $Ca(OH)_2$ and EDTA. Conclusions: The application of $Ca(OH)_2$ and additional treatment such as EDTA or instrumentation promoted the attachment and differentiation of DPSCs after NaOCl treatment.
Park Minjeong,Shin Eunhye,Hong Jongin,Paik Hanjong 한국물리학회 2020 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.77 No.12
β-Ni(OH)2 and NiO nanostructured films were prepared on fluorine-doped tin oxide (FTO) glass substrates by using chemical bath deposition (CBD) at different concentrations of a complexing agent (i.e., NH3). The as-deposited β-Ni(OH)2 films exhibited various porous structures, such as nanoislands, nanoflakes, nanosheets and nanoplatelets, depending on the concentration of the complexing agent, and they could be converted to NiO films by using calcination. The β-Ni(OH)2 and the NiO films were characterized by using scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS). Importantly, the electrochemical activity of NiO nanoplatelet-films for the oxygen evolution reaction (OER) was also investigated as a function of the annealing temperature.
MinJeong Kim,SungGon Kim,HyoJeong Kim,HoChan Kim,JiHeh Park,KwangSeok Park,DukKi Lee,WonTan Byun,CheolMin Kim 대한신경정신의학회 2008 PSYCHIATRY INVESTIGATION Vol.5 No.3
Objective-The Penn Alcohol Craving Scale (PACS) is a stronger predictor of subsequent drinking and relapse of alcohol dependence that can be administered more quickly and easily than other craving scales. The goal of this study was to develop the Korean version of the Penn Alcohol Craving Scale (PACS-K). Methods-To examine the psychometric properties of the PACS-K, responses were chosen from 80 patients admitted to a treatment facility for alcohol dependence. Results-The PACS-K possesses good psychometric properties, as assessed by Cronbach’s α estimates (Cronbach’s α=0.91). The test-retest reliability of the PACS-K showed high correlation (p<0.01) when the retest interval was 1 day. When the validity of the PACS-K was investigated using correlation analysis with two other craving scales (the Obsessive Compulsive Drinking Scale (OCDS) and the Visual Analogue Scale (VAS), high correlations were obtained between total PACS scores and total OCDS scores, and between total PACS scores and VAS scores (p<0.01, respectively). Conclusion-The PACS-K is a reliable and valid measure of alcohol cravings, and it could be useful for predicting which individuals are at risk for subsequent relapse.