piRNA-1742 promotes renal cell carcinoma malignancy by regulating USP8 stability through binding to hnRNPU and thereby inhibiting MUC12 ubiquitination

Zhang Wentao,Zheng Zongtai,Wang Keyi,Mao Weipu,Li Xue,Wang Guangchun,Zhang Yuanyuan,Huang Jianhua,Zhang Ning,Wu Pengfei,Liu Ji,Zhang Haimin,Che Jianping,Peng Bo,Zheng Junhua,Li Wei,Yao Xudong 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Accumulating studies have confirmed that PIWI-interacting RNAs (piRNAs) are considered epigenetic effectors in cancer. We performed piRNA microarray expression analysis on renal cell carcinoma (RCC) tumor tissues and paired normal tissues and performed a series of in vivo and in vitro experiments to explore piRNAs associated with RCC progression and investigate their functional mechanisms. We found that piR-1742 was highly expressed in RCC tumors and that patients with high piR-1742 expression had a poor prognosis. Inhibition of piR-1742 significantly reduced tumor growth in RCC xenograft and organoid models. Mechanistically, piRNA-1742 regulates the stability of USP8 mRNA by binding directly to hnRNPU, which acts as a deubiquitinating enzyme that inhibits the ubiquitination of MUC12 and promotes the development of malignant RCC. Subsequently, nanotherapeutic systems loaded with piRNA-1742 inhibitors were found to effectively inhibit the metastasis and growth of RCC in vivo. Therefore, this study highlights the functional importance of piRNA-related ubiquitination in RCC and demonstrates the development of a related nanotherapeutic system, possibly contributing to the development of therapeutic approaches for RCC.

Review on State of Charge Estimation Methods for Li-Ion Batteries

Zhang, Xiaoqiang,Zhang, Weiping,Li, Hongyu,Zhang, Mao The Korean Institute of Electrical and Electronic 2017 Transactions on Electrical and Electronic Material Vol.18 No.3

The state of charge (SOC) is an important parameter in a battery-management system (BMS), and is very significant for accurately estimating the SOC of a battery. Li-ion batteries boast of excellent performance, and can only remain at their best working state by means of accurate SOC estimation that gives full play to their performances and raises their economic benefits. This paper summarizes some measures taken in SOC estimation, including the discharge experiment method, the ampere-hour integral method, the open circuit voltage method, the Kalman filter method, the neural network method, and electrochemical impedance spectroscopy (EIS. The principles of the various SOC estimation methods are introduced, and their advantages and disadvantages, as well as the working conditions adopted during these methods, are discussed and analyzed.

Trans-arterial Chemo-Embolization in Treating Elderly Patients with Hepatocellular Carcinoma

Zhang, Jian-Feng,Liu, Jun-Mao,Zhang, Nin,Du, Chao,Zheng, Qin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16

Background: This analysis was conducted to evaluate the efficacy and safety of Trans-arterial Chemo-Embolization (TACE) in treating Elderly patients with Hepatocellular Carcinoma (EHPC). Methods: Clinical studies evaluating the efficacy and safety of TACE on response and safety for patients with EHPC were identified by using a predefined search strategy. Pooled response rate of treatment were calculated. Results: In TACE based regimen, clinical studies which including patients with EHPC were considered eligible for the evaluation of response. And, in these TACE based treatments, pooled analysis suggested that, in all 288 patients whose response could be assessed, the pooled reponse rate was 29.5%(85/288) in TACE based treatment. The most commonly encountered TACE-related morbidity was liver function impairment. No grade III or IV renal or liver toxicity were observed. No treatment related death occurred in EHPC patients with TACE based treatments. Conclusion: This evidence based analysis suggests that TACE based treatments are associated with mild response rate and accepted toxicities for treating patients with EHPC.

Thermo-mechanical coupling behavior analysis for a U-10Mo/Al monolithic fuel assembly

Mao, Xiaoxiao,Jian, Xiaobin,Wang, Haoyu,Zhang, Jingyu,Zhang, Jibin,Yan, Feng,Wei, Hongyang,Ding, Shurong,Li, Yuanming Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.9

A typical three-dimensional finite element model for a fuel assembly is established, which is composed of 16 monolithic U-10Mo fuel plates and Al alloy frame. The distribution and evolution results of temperature, displacement and stresses/strains in all the parts are numerically obtained and analyzed with a self-developed code of FUELTM. The simulation results indicate that (1) the out-of-plane displacements of Al alloy side plates are mainly attributed to the bending deformations; (2) enhanced out-of-plane displacements appear in fuel plates adjacent to the outside Al plates, which results from the occurred bending deformations due to the applied constraints of outside Al plates; (3) an intense interaction of fuel foil with the cladding occurs near the foil edge, which appears more heavily in the fuel plates adjacent to the outside Al plates. The maximum first principal stresses in the fuel foil are similar for all the fuel plates and appear near the fuel foil edge; while, the through-thickness creep strains of fuel foil in the fuel plate near the central region of fuel assembly are larger, and the induced creep damage might weaken the fuel skeleton strength and raise the fuel failure risk.

A Novel Model of a Li-ion Battery Based on the Manufacturer's Datasheet

Zhang, Xiaoqiang,Zhang, Weiping,Zhang, Mao The Korean Institute of Electrical and Electronic 2017 Transactions on Electrical and Electronic Material Vol.18 No.5

A novel battery model based on the manufacturer datasheet is proposed. According to this model, not only the steady state but also the dynamic charging performance of the Li-ion battery can be analyzed and evaluated. The major advantage of our model is that all the parameters can be directly obtained from the datasheet and no additional experiments are required. Moreover, the transition between charge and discharge stages was analyzed based on our model, and a novel Simulink module was built to predict the energy consumption of a battery-powered system. Experiments were carried out to verify the model accuracy. Although the new model was developed for the Li-ion battery, it is expected to be applicable to other batteries.

Ruin probabilities in the risk model with two compound Binomial processes

Mao-Jun Zhang,Jiang-Xia Nan,Sen Wang 한국전산응용수학회 2008 Journal of applied mathematics & informatics Vol.26 No.1

In this paper, we consider an insurance risk model governed by a compound Binomial arrival claim process and by a compound Binomial arrival premium process. Some formulas for the probabilities of ruin and the distribution of ruin time are given, we also prove the integral equation of the ultimate ruin probability and obtain the Lundberg inequality by the discrete martingale approach.

Response improvement for In2O3–TiO2 thick film gas sensors

Mao Lin Zhang,Jian Ping Song,Zhan Heng Yuan,Cheng Zheng 한국물리학회 2012 Current Applied Physics Vol.12 No.3

In2O3 is introduced into TiO2 by sol-gel method to improve the response/recovery rate and expand the operating temperature, when the In2O3-TiO2 mixed system is exposed to H2/O2. The sensor is fabricated by thick film technology. Influence of In2O3 on the film phase composition, microstructure and sensing characteristics is discussed. Dynamic response properties show that the operating temperature of the mixed system is at 500e800 ℃, which is about 600e800 ℃ for pure TiO2. Response time of the sensor is about 200e260 ms (millisecond) while recovery time is in a narrow range of 60e280 ms at 600e800 ℃. The promoting mechanism is suggested to arise from the introduction of In2O3 and grain size effect of the sensing film. Then In2O3-TiO2 thick films are surface-modified by Pt using chloroplatinic acid. The promoting effect of Pt dispersed on the mixed system is also investigated. In2O3 is introduced into TiO2 by sol-gel method to improve the response/recovery rate and expand the operating temperature, when the In2O3-TiO2 mixed system is exposed to H2/O2. The sensor is fabricated by thick film technology. Influence of In2O3 on the film phase composition, microstructure and sensing characteristics is discussed. Dynamic response properties show that the operating temperature of the mixed system is at 500e800 ℃, which is about 600e800 ℃ for pure TiO2. Response time of the sensor is about 200e260 ms (millisecond) while recovery time is in a narrow range of 60e280 ms at 600e800 ℃. The promoting mechanism is suggested to arise from the introduction of In2O3 and grain size effect of the sensing film. Then In2O3-TiO2 thick films are surface-modified by Pt using chloroplatinic acid. The promoting effect of Pt dispersed on the mixed system is also investigated.

Analysis of Laparoscopy-assisted Gastric Cancer Operations Performed by Inexperienced Junior Surgeons

Zhang, Xing-Mao,Wang, Zheng,Liang, Jian-Wei,Zhou, Zhi-Xiang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

To clarify whether gastric cancer patients can benefit from laparoscopy-assisted surgery completed by junior surgeons under supervision of expert surgeons, data of 232 patients with gastric cancer underwent operation performed by inexperienced junior surgeons were reviewed. Of the 232 patients, 137 underwent laparoscopy-assisted resection and in 118 cases this approach was successful. All of these 118 patients were assigned to laparoscopic group in this study, 19 patients who were switched to open resection were excluded. All laparoscopic operations were performed under the supervision of expert laparoscopic surgeons. Some 95 patients receiving open resection were assigned to the open group. All open operations were completed independently by the same surgeons. Short-term outcomes including oncologic outcomes, operative time intra-operative blood loss, time to first flatus, time to first defecation, postoperative hospital stay and perioperative complication were compared between the two groups. The numbers of lymph nodes harvested in the laparoscopic and open groups were $21.1{\pm}9.6$ and $18.2{\pm}9.7$ (p=0.029). There was no significant difference in the length of margins. The mean operative time was $215.9{\pm}32.2$ min in laparoscopic group and $220.1{\pm}34.6min$ in the open group (p=0.866), and the mean blood loss in laparoscopic group was obviously less than that in open group ($200.9{\pm}197.0ml$ vs $291.1{\pm}191.4ml$; p=0.001). Time to first flatus in laparoscopic and open groups was $4.0{\pm}1.0$ days and $4.3{\pm}1.2$ days respectively and the difference was not significant (p=0.135). Similarly no statically significant difference was noted for time to first defecation ($4.7{\pm}1.6$ vs $4.8{\pm}1.6$, p=0.586). Eleven patients in the laparoscopic group and 19 in the open group suffered from peri-operative complications and the difference between the two groups was significant (9.3% vs 20.0%, p=0.026). The conversion rate for laparoscopic surgery was 13.9%. Patients with gastric cancer can benefit from laparoscopy-assisted operations completed by inexperienced junior surgeons under supervision of expert laparoscopic surgeons.

Advantages of Laparoscopic Abdominoperineal Resection for Anastomotic Recurrence of Rectal Cancer

Zhang, Xing-Mao,Wang, Zheng,Ma, Sheng-Hui,Zhou, Zhi-Xiang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10

Background: Surgery offers the only potential for cure and long-term survival of recurrence of rectal cancer. Few studies about laparoscopic recurrent lesion resection have been reported. This study was designed to evaluate the safety and feasibility of laparoscopic abdomino-perineal resection for anastomotic recurrence of rectal cancer. Materials and Methods: Data for 42 patients with recurrence of rectal cancer were collected retrospectively. Of the 42 patients, 22 underwent laparoscopic surgery (LR group) and 20 received open surgery (OR group). Outcomes between the two groups were compared. Results: Operation time in LR group was shorter compared with the OR group ($164.6{\pm}27.7min$ vs $203.0{\pm}45.3min$); intra-operative blood loss was $119.7{\pm}44.4ml$ and $185.0{\cdot}94.0ml$ in LR group and OR group, respectively (p<0.001); time to first flatus in LR group was shorter than in OR group, and the difference was statistically significant ($2.6{\pm}0.8$ days vs $3.1{\pm}0.8$ days, p=0.013); hospital stay in the LR and OR groups was $8.6{\pm}1.3$ days and $9.8{\pm}2.2$ days; 3-year survival rates in the LR and OR groups were 44.4% and 42.8% (p=0.915) and the 3-year disease-free survival rates were 36.4% and 30.0%, respectively (p=0.737). Conclusions: Laparoscopic abdomino-perineal resection is safe and feasible for anastomotic recurrence of rectal cancer.

A New Unsymmetrical Zinc Phthalocyanine as Photosensitizers for Dye-sensitized Solar Cells

Zhang, Dan,Zhang, Xue-Jun,Zhang, Lei,Mao, Li-Jun Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.4

A new unsymmetrical zinc phthalocyanine has been designed and synthesized based on the 'push-pull' and extended ${\pi}$-conjugation concept for the dye-sensitized solar cells. Three tert-butoxy groups, which act as electron releasing ('push'), enhance the solubility of phthalocyanine in common organic solvents and reduce the aggregation. Hydroxy substituted 9,10-anthraquinones act as electron acceptors ('pull') for the study of photoinduced electron transfer processes as well as grafting onto nanocrystalline $TiO_2$. The new unsymmetrical zinc phthalocyanine was fully characterized by FTIR, UV-vis, $^1H$ NMR, cyclic voltammetry and differential pulse voltammetry. The new sensitizer was tested in dye-sensitized solar cells, and gave a better performance.