MicroRNA Expression Profile Analysis Reveals Diagnostic Biomarker for Human Prostate Cancer

Liu, Dong-Fu,Wu, Ji-Tao,Wang, Jian-Ming,Liu, Qing-Zuo,Gao, Zhen-Li,Liu, Yun-Xiang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Prostate cancer is a highly prevalent disease in older men of the western world. MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression via posttranscriptional inhibition of protein synthesis. To identify the diagnostic potential of miRNAs in prostate cancer, we downloaded the miRNA expression profile of prostate cancer from the GEO database and analysed the differentially expressed miRNAs (DE-miRNAs) in prostate cancerous tissue compared to non-cancerous tissue. Then, the targets of these DE-miRNAs were extracted from the database and mapped to the STRING and KEGG databases for network construction and pathway enrichment analysis. We identified a total of 16 miRNAs that showed a significant differential expression in cancer samples. A total of 9 target genes corresponding to 3 DE-miRNAs were obtained. After network and pathway enrichment analysis, we finally demonstrated that miR-20 appears to play an important role in the regulation of prostate cancer onset. MiR-20 as single biomarker or in combination could be useful in the diagnosis of prostate cancer. We anticipate our study could provide the groundwork for further experiments.

Effect of a New Prokinetic Agent DA-9701 Formulated with Corydalis Tuber and Pharbitidis Semen on Cytochrome P450 and UDP-Glucuronosyltransferase Enzyme Activities in Human Liver Microsomes

Ji, Hye Young,Liu, Kwang Hyeon,Jeong, Ji Hyeon,Lee, Dae-Young,Shim, Hyun Joo,Son, Miwon,Lee, Hye Suk Hindawi Publishing Corporation 2012 Evidence-based Complementary and Alternative Medic Vol.2012 No.-

<P>DA-9701 is a new botanical drug composed of the extracts of Corydalis tuber and Pharbitidis semen, and it is used as an oral therapy for the treatment of functional dyspepsia in Korea. The inhibitory potentials of DA-9701 and its component herbs, Corydalis tuber and Pharbitidis semen, on the activities of seven major human cytochrome P450 (CYP) enzymes and four UDP-glucuronosyltransferase (UGT) enzymes in human liver microsomes were investigated using liquid chromatography-tandem mass spectrometry. DA-9701 and Corydalis tuber extract slightly inhibited UGT1A1-mediated etoposide glucuronidation, with 50% inhibitory concentration (IC<SUB>50</SUB>) values of 188 and 290 <I>μ</I>g/mL, respectively. DA-9701 inhibited CYP2D6-catalyzed bufuralol 1′-hydroxylation with an inhibition constant (<I>K</I><SUB>i</SUB>) value of 6.3 <I>μ</I>g/mL in a noncompetitive manner. Corydalis tuber extract competitively inhibited CYP2D6-mediated bufuralol 1′-hydroxylation, with a <I>K</I><SUB>i</SUB> value of 3.7 <I>μ</I>g/mL, whereas Pharbitidis semen extract showed no inhibition. The volume in which the dose could be diluted to generate an IC<SUB>50</SUB> equivalent concentration (volume per dose index) value of DA-9701 for inhibition of CYP2D6 activity was 1.16 L/dose, indicating that DA-9701 may not be a potent CYP2D6 inhibitor. Further clinical studies are warranted to evaluate the <I>in vivo</I> extent of the observed <I>in vitro</I> interactions.</P>

녹차 카테킨의 자유 라디칼(Free Radicals) 소거(消去) 메커니즘

Liu Ji Chen,Xian Qiang Yang,Park Jae Il,Sheng Rong Shen,Yue Fei Wang 한국식품과학회 2001 국제녹차심포지엄 Vol.2001 No.1

EXISTENCE RESULTS FOR POSITIVE SOLUTIONS OF NON-HOMOGENEOUS BVPS FOR SECOND ORDER DIFFERENCE EQUATIONS WITH ONE-DIMENSIONAL p-LAPLACIAN

Liu, Yu-Ji Korean Mathematical Society 2010 대한수학회지 Vol.47 No.1

Motivated by [Science in China (Ser. A Mathematics) 36 (2006), no. 7, 721?732], this article deals with the following discrete type BVP $\LARGE\left\{{{\;{\Delta}[{\phi}({\Delta}x(n))]\;+\;f(n,\;x(n\;+\;1),{\Delta}x(n),{\Delta}x(n + 1))\;=\;0,\;n\;{\in}\;[0,N],}}\\{\;{x(0)-{\sum}^m_{i=1}{\alpha}_ix(n_i) = A,}}\\{\;{x(N+2)-\;{\sum}^m_{i=1}{\beta}_ix(n_i)\;=\;B.}}\right.$ The sufficient conditions to guarantee the existence of at least three positive solutions of the above multi-point boundary value problem are established by using a new fixed point theorem obtained in [5]. An example is presented to illustrate the main result. It is the purpose of this paper to show that the approach to get positive solutions of BVPs by using multifixed-point theorems can be extended to treat nonhomogeneous BVPs. The emphasis is put on the nonlinear term f involved with the first order delta operator ${\Delta}$x(n).

Electrochemical Preparation of Co₃Pt Nanowires

Ji Hyun Min,Jun-Hua Wu,Ji Ung Cho,Ju Hun Lee,Young-Dong Ko,Seung Pil Ko,Hong-Ling Liu,Jin-Seok Chung,Young Keun Kim 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

<i>In Situ</i> Activation of Nitrogen-Doped Graphene Anchored on Graphite Foam for a High-Capacity Anode

Ji, Junyi,Liu, Jilei,Lai, Linfei,Zhao, Xin,Zhen, Yongda,Lin, Jianyi,Zhu, Yanwu,Ji, Hengxing,Zhang, Li Li,Ruoff, Rodney S. American Chemical Society 2015 ACS NANO Vol.9 No.8

<P>We report the fabrication of a three-dimensional free-standing nitrogen-doped porous graphene/graphite foam by <I>in situ</I> activation of nitrogen-doped graphene on highly conductive graphite foam (GF). After <I>in situ</I> activation, intimate “sheet contact” was observed between the graphene sheets and the GF. The sheet contact produced by <I>in situ</I> activation is found to be superior to the “point contact” obtained by the traditional drop-casting method and facilitates electron transfer. Due to the intimate contact as well as the use of an ultralight GF current collector, the composite electrode delivers a gravimetric capacity of 642 mAh g<SUP>–1</SUP> and a volumetric capacity of 602 mAh cm<SUP>–3</SUP> with respect to the whole electrode mass and volume (including the active materials and the GF current collector). When normalized based on the mass of the active material, the composite electrode delivers a high specific capacity of up to 1687 mAh g<SUP>–1</SUP>, which is superior to that of most graphene-based electrodes. Also, after ∼90 s charging, the anode delivers a capacity of about 100 mAh g<SUP>–1</SUP> (with respect to the total mass of the electrode), indicating its potential use in high-rate lithium-ion batteries.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2015/ancac3.2015.9.issue-8/acsnano.5b03888/production/images/medium/nn-2015-03888k_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn5b03888'>ACS Electronic Supporting Info</A></P>

Effect of bone boiling duration on bone extract supplement quality for broilers as to growth performance, leg bone length, and blood profile

Ji-Hwan Lee,Chang-Hee Lee,Seo-Young Oh,Woo-Gi Kwak,Han-Jin Oh,Won Yun,Jin-Kyu Lee,Ji-Taek Jeong,Yeong-Seok Choi,Shu-Dong Liu,Yang-Il Choi,Jin-Ho Cho 충남대학교 농업과학연구소 2017 Korean Journal of Agricultural Science Vol.44 No.1

A Study on method of designing developable surface

Ji-xin YANG,Zhe LIU,Jian LIU 한국멀티미디어학회 2006 한국멀티미디어학회 국제학술대회 Vol.2006 No.-

The paper deals with the principle of envelope of a one-parameter plane family to design developable surface. Three methods of designing developable surface are presented. They are designing a developable surface based on one curve on it and its normal line, designing a developable surface based on two curves on it and designing a developable surface based on one curve and one surface. They meet the requirements of engineering fields.

Analysis of Mammographic Breast Density in a Group of Screening Chinese Women and Breast Cancer Patients

Liu, Jing,Liu, Pei-Fang,Li, Jun-Nan,Qing, Chun,Ji, Yu,Hao, Xi-Shan,Zhang, Xue-Ning Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: A dense breast not only reduces the sensitivity of mammography but also is a moderate independent risk factor for breast cancer. The percentage of Western women with fat breast tissue is higher aged 40 years or older. To a certain extent, mammography as a first choice of screening imaging method for Western women of this group is reasonable. Hitherto, the frequency and age distribution of mammographic breast density patterns among Chinese women had not been characterized. The purpose of this study was to investigate the frequency and age distribution of mammographic breast density patterns among a group of Chinese screening women and breast cancer patients in order to provide useful information for age-specific guidelines for breast cancer screening in Chinese women. Methods: A retrospective review of a total of 3,394 screening women between August and December 2009 and 2,527 breast cancer patients between July 2011 and June 2012 was conducted. Descriptive analyses were used to examine the association between age and breast density. The significance of differences of breast density between the screening women and the breast cancer patients was examined using nonparametric tests. Results: There was a significant inverse relationship between age and breast density overall (r=-0.37, p< 0.01). Breast density of the breast cancer patients in the subgroups of 40-49 years old was greater compared with that of the screening women, the same in those aged 50-54 years and in those 55 years old or older, less than in the screening group. Conclusions: With regard to the Chinese women younger than 55 years old, the diagnostic efficiency of breast cancer screening imaging examinations may be potentially improved by combining screening mammography with ultrasound.

Population genetics reveals new introgression in the nucleus herd of min pigs

Liu Tianxin,Ji Dongqing,Li Xinyuan,Liu Jiadong,Xu Fei,Miao Zhiying,Chang Yang,Tian Ming,Xu Chunzhu 한국유전학회 2024 Genes & Genomics Vol.46 No.4

Objective Min pigs are a unique genetic resource among local pig breeds in China. They have more excellent characteristics in cold and stress resistance, good meat quality, and a high reproductive rate. However, the genetic structure and driving factors remain unclear in the nucleus herd. In this study, the genetic diversity of Min pigs was studied to reveal the formation mechanism of its unique genetic structure. We hope to protect and develop the genetic resources of Min pigs. Methods We analyzed different types of genes to identify the genetic structure and gene introgression pattern of Min pigs. The nuclear DNA dataset includes information on 21 microsatellite loci and 6 Y-chromosome genes, and the mitochondrial D-loop gene is selected to represent maternal lineages. The above genes are all from the nucleus herd of Min pigs. Results The results of genetic structure identification and analysis of potential exogenous gene introgression patterns indicate that the nucleus herd of Min pigs maintains a high level of genetic diversity (polymorphism information content = 0.713, expected heterozygosity = 0.662, observed heterozygosity = 0.612). Compared with other Asian pig breeds, the formation of Min pig breeds is more special. Gene introgression from European pig breeds to Min pigs has occurred, which is characterized by complete introgression of paternal genes and incomplete introgression of maternal genes. Conclusion Gene introgression caused by cross-breeding is not the main factor leading to the formation of the current genetic structure of Min pigs, but this process has increased the level of genetic diversity in the nucleus herd. Compared with the influence of gene introgression, our research suggest that artificial selection and environmental adaptive evolution make Min pigs form unique genetic characteristics. Objective Min pigs are a unique genetic resource among local pig breeds in China. They have more excellent characteristics in cold and stress resistance, good meat quality, and a high reproductive rate. However, the genetic structure and driving factors remain unclear in the nucleus herd. In this study, the genetic diversity of Min pigs was studied to reveal the formation mechanism of its unique genetic structure. We hope to protect and develop the genetic resources of Min pigs. Methods We analyzed different types of genes to identify the genetic structure and gene introgression pattern of Min pigs. The nuclear DNA dataset includes information on 21 microsatellite loci and 6 Y-chromosome genes, and the mitochondrial D-loop gene is selected to represent maternal lineages. The above genes are all from the nucleus herd of Min pigs. Results The results of genetic structure identification and analysis of potential exogenous gene introgression patterns indicate that the nucleus herd of Min pigs maintains a high level of genetic diversity (polymorphism information content = 0.713, expected heterozygosity = 0.662, observed heterozygosity = 0.612). Compared with other Asian pig breeds, the formation of Min pig breeds is more special. Gene introgression from European pig breeds to Min pigs has occurred, which is characterized by complete introgression of paternal genes and incomplete introgression of maternal genes. Conclusion Gene introgression caused by cross-breeding is not the main factor leading to the formation of the current genetic structure of Min pigs, but this process has increased the level of genetic diversity in the nucleus herd. Compared with the influence of gene introgression, our research suggest that artificial selection and environmental adaptive evolution make Min pigs form unique genetic characteristics.