韓國人에서 VNTR D1S80 遺傳座位의 遺傳的 多樣性 및 集團의 均質性 檢定

明賢君,黃迪駿,洪鎔杓,金致弘 大韓法醫學會 1994 대한법의학회지 Vol.18 No.1

Human genomic DNA prepared from whole blood sample was analyzed to estimate allelic frequencies and genetic diversity at a hypervariable variable number of tandem repeats(VNTR) D1S80 locus in 127 Korean. Target DNA fragments were amplified by polymerase chain reaction(PCR) and separated by polyacrylamide gel electrophoresis. Then, the following results were obtained. 1. A total of 23 alleles were identified at this locus, which is the biggest number of alleles observed at this locus so far(cf. 16 from U. S. Caucasians and 15 from Finnish). 2. Sixty-two genotypes, included 3 homozygotes and 59 heterozygotes, out of a total of 276 probable genotypes were also observed, which might be resulted from the small sample size compared to the number of alleles observed in this study. 3. General pattern of allelic distributions was concordant with those observed in previous studies from Finnish and U. S. Causccasians where two alleles, M18 and M23, were observed most frequently and the rest of them were observed rare(<10%) with the exception of M30 allele(14.6%) in Korean. The number of M30 allele is suspected to be increased in Korean at or after the divergence of Korean from the hypothetical ancestor probably caused by random genetic drift such as founder effect of bottleneck effect. 4. Higher level of heterozygosity was obtained in Korean(H=0.866 and H=0.881) than in Finnish(H=0.77 and H=0.79) and U. S. Cauccasians(H=0.808 and H=0.797). 5. Population homogeneity was tested to corroborate the obtained population genetic parameters calculated based on the assumption of Hardy-Weinberg equilibrium, which could be done by Chi-square test between the observed number of alleles and the expected number of alleles, because of the small sample size analyzed in this study which resulted in high proportion of undetected genotypes (78.5%) and several genotypes significantly deviated from hardy-Weinberg expectation in a sample population. The result suggests that samples were drawn from geneticically homogeneous population. 6. This study suggests that Korean is more diversified than Finnish and U. S. Cauccasians on the basis of the number of alleles and the level of heterozygosity, although Korean is known to be entirely composed of single race. Though we need more informations from other Asian countries, this result suggests frequent gene flow from neighbor countries, which is also expected based on the old Korean history of frequent invasions from Japan and China. 7. Based on the Hardy-Weinberg expectation, 216 out of 253 genotypes at D1S80 locus can be shared by less than 5 individuals among 1,000 unrelated individuals by chance. Our results suggest that the analysis of VNTR D1S80 locus can provide powerful, but imperfect by itself, DNA markers for forensic study, which can be corroborated by analyzing additional VNTR loci.

Coagulant와 Hydro-cyclone을 결합한 상향류식 여과시스템

김명호,고대현,정다래,이준호 한국교통대학교 2021 한국교통대학교 논문집 Vol.56 No.-

Coagulation Hydro-cyclone can be added to the upward flow filtration system utilizing Fiber-Ball filter media to analyze and evaluate the treatment characteristics in order to Non-point pollution, which is a pollution problem, more efficiently. Hydro-cyclone was added to the existing Filter column to create an Up-Flow Filtration system. The treatment efficiency according to the operating conditions such as turbidity, suspended solids, pH, inflow flow rate, and head loss was analyzed. Comparison of inflow water and runoff water suspended solids and turbidity analysis results showed a minimum treatment efficiency of 93.21% and a maximum treatment efficiency of 99% or more. It was analyzed that the SS and turbidity analysis results showed a treatment efficiency of 80% or more before and after backwashing twice. When Coagulation Hydro-cyclone was added compared to the existing filtration system, it was analyzed that particles that had not been treated as an adsorption fiber company filter medium between particles could be treated.

에어리프트 펌프를 이용한 정밀여과막 세척

이준호,김명호,고대현 韓國交通大學校 2022 한국교통대학교 논문집 Vol.57 No.-

The MBR method, also known as the membrane separation active sludge method, does not require a precipitation tank and has many advantages such as stable discharging water. The biggest disadvantages of the MBR method, which has many advantages, are maintenance problems due to reduced flux due to fouling, decreased processing efficiency, and membrane replacement. In this study, in order to reduce membrane pollution, membrane cleaning was performed using the washing principle of the airlift pump, and shear force according to the amount of air was analyzed using the Ansys Fluent computational fluid dynamics program. As a result of the analysis, the film recovery rate was 93.8% to 97.3% and the average was 95.8%, and the CFD analysis showed the highest shear force at about 5.425×10-2Pa at 10m3/m2/hr. It is believed that the MBR device using Airlift in this study can maximize the flux recovery rate by reducing the problem of membrane contamination.

Pretreated fucoidan confers neuroprotection against transient global cerebral ischemic injury in the gerbil hippocampal CA1 area via reducing of glial cell activation and oxidative stress

Kim, Hyunjung,Ahn, Ji Hyeon,Song, Minah,Kim, Dae Won,Lee, Tae-Kyeong,Lee, Jae-Chul,Kim, Young-Myeong,Kim, Jong-Dai,Cho, Jun Hwi,Hwang, In Koo,Yan, Bing Chun,Won, Moo-Ho,Park, Joon Ha Elsevier 2019 BIOMEDICINE AND PHARMACOTHERAPY Vol.109 No.-

<P><B>Abstract</B></P> <P>Fucoidan is a sulfated polysaccharide derived from brown algae and possesses various beneficial activities, including antioxidant property. Previous studies have shown that fucoidan displays protective effect against ischemia-reperfusion injury in some organs. However, few studies have been reported regarding the protective effect of fucoidan against transient cerebral ischemic insults and its related mechanisms. Therefore, in this study, we examined the neuroprotective effect of fucoidan against transient global cerebral ischemia (tGCI), as well as underlying its mechanism using a gerbil model of tGCI which shows a loss of pyramidal neurons in the hippocampal cornu ammonis 1 (CA1) area after 5 min of tGCI. Fucoidan (25 and 50 mg/kg) was intraperitoneally administered once daily for 5 days before tGCI. Pretreatment with 50 mg/kg of fucoidan, not 25 mg/kg of fucoidan, attenuated tGCI-induced hyperactivity and protected CA1 pyramidal neurons from tGCI. In addition, pretreatment with 50 mg/kg of fucoidan inhibited activations of astrocytes and microglia in the ischemic CA1 area. Furthermore, pretreatment with 50 mg/kg of fucoidan significantly reduced the increased 4-hydroxy-2-noneal and superoxide anion radical production in the ischemic CA1 area and significantly increased expressions of SOD1 and SOD2 in the CA1 pyramidal neurons before and after tGCI. Additionally, treatment with diethyldithiocarbamate (an inhibitor of SODs) to the fucoidan-treated gerbils notably abolished the fucoidan-mediated neuroprotection. In brief, our present results indicate that fucoidan can effectively protect neurons from tGCI through attenuation of activated glial cells and reduction of oxidative stress via increase of SODs. Thus, we strongly suggest that fucoidan can be used as a useful preventive agent in cerebral ischemia.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pretreatment with fucoidan protects CA1 pyramidal neurons from ischemic damage. </LI> <LI> Pretreated fucoidan inhibits activation of glial cells in ischemic CA1 area. </LI> <LI> Pretreated fucoidan attenuates oxidative stress in CA1 area after ischemic insult. </LI> <LI> Pretreated fucoidan increases SODs expressions in ischemic CA1 pyramidal neurons. </LI> <LI> Fucoidan-mediated neuroprotection is abolished by DDC (SODs inhibitor) treatment. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Validation of Calendar-based Spray Programs for Controlling Diseases and Pests of Strawberry during Nursery Seasons

Myeong Hyeon Nam,Hyun Sook Kim,Tae Il Kim,Eun Mo Lee,Hong Gi Kim 한국원예학회 2015 한국원예학회 학술발표요지 Vol.2015 No.5

Timing of Conventional and Microbial Fungicide Applications for Botrytis cinerea in Strawberry Fruit

Myeong-Hyeon Nam,Hyun-Sook Kim,Won-Keun Lee,Mark Gleason,Hong-Gi Kim 한국원예학회 2009 한국원예학회 학술발표요지 Vol.2009 No.10

Application of an IPM-based Spray Program to Protected Cultivation of Strawberry in Korea

Myeong Hyeon Nam,Hyeon Suk Kim,Won Keun Lee,Yeol Kyu Seong,Mark. L. Gleason,Jeong Young Song,Hong Gi Kim 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.5

An IPM-based spray program (ISP), based on ecological and agronomic information, is necessary for reducing pesticide sprays and producing strawberries safely. In this study, the applicability of ISP to safe production of strawberries was compared with a conventional spray program (Con-SP) during the 2005-2006 and 2006-2007 seasons in plastic houses in Nonsan, Korea. The ISP program consisted of fungicide, insecticide, and miticide treatments as well as release of natural enemies before and after harvest. The ISP treatment schedule was as follows: dipping transplants in azoxystrobin before transplanting (mid-September); spraying with abamectin and thiacloprid when black mulch was applied (early October); spraying with pyraclostrobin + acetamiprid and tetraconazole + abamectin before blooming in early and mid-November, respectively; transplanting banker plants colonized by Aphidius colemani in mid-February (2005-2006 season) or November (2006-2007 season); and releasing Phytoseiulus persimilis in mid-March. Number of pesticide sprays per season for ISP and Con-SP was five and nine, respectively. Anthracnose crown rot, caused by Colletotrichum gloeosporioides, did not occur in ISP in either season, but incidence was ≤ 3.3% in Con-SP during the 2005-2006 season. Powdery mildew (Sphaerotheca aphanis var. aphanis) did not occur during harvest in ISP, but occurred during early and late harvest stages in Con-SP in both years. Incidence of Botrytis fruit rot did not differ between ISP and Con-SP. Two-spotted spider mite (Tetranychus urticae) did not appear until March in ISP; subsequently, spider mite density remained at ≤-0.5 mite per leaf until the last harvest period in both years. The number of spider mites in ISP and Con-SP during the harvest did not differ. Populations of cotton aphids (Aphis gossypii) were also higher in ISP than Con-SP but decreased from March to April, accompanied by production of aphid mummies by A. colemani. The results showed that ISP was more effective than Con-SP, and required fewer pesticide sprays.

A Systematic Diagnosis of Systemic Risk

Myeong Hyeon Kim,Baeho Kim 한국재무학회 2012 한국재무학회 학술대회 Vol.2012 No.05

This paper proposes a holistic and comprehensive approach to accurately measure and detect systemic risk. A counter-cyclical structure of regulatory policy has been widely proposed as a countermeasure of the pro-cyclicality of the risk charge. Accordingly, extant regulatory approaches highlight the need for leading variables as a harbinger of a systemic crisis based on the market prices rather than lagging ones. Our empirical study based on a Markov regime-switching model conrms that both leading and lagging systemic variables are helpful in a complementary manner. The proposed diagnostic framework clearly demonstrates the dierent aspects of a series of previous crises.

Effect of Fungicides in the Control of Anthracnose of Strawberry Plants Caused by Colletotrichum gloeosporioides

Myeong Hyeon Nam,Hyeon Suk Kim,In Ha Lee,Won Keun Lee,Yeol Kyu Seong,Natalia Peres 한국원예학회 2008 한국원예학회 기타간행물 Vol.- No.-

Highly efficient non-doped deep blue fluorescent emitters with horizontal emitting dipoles using interconnecting units between chromophores

Kim, Kwon-Hyeon,Baek, Jang Yeol,Cheon, Chan Woo,Moon, Chang-Ki,Sim, Bomi,Choi, Myeong Yong,Kim, Jang-Joo,Kim, Yun-Hi The Royal Society of Chemistry 2016 Chemical communications Vol.52 No.73

<P>New deep blue fluorescent emitters composed of anthracene as an electron rich unit, a diphenyltriazine as a strong electron acceptor unit, and phenyl or xylene as interconnecting units were synthesised. The interconnecting unit between chromophores increased the singlet transition energy and the ratio of horizontal emitting dipoles. As a result, a non-doped blue fluorescent organic light-emitting diode (OLED) using a new emitter was demonstrated, with an external quantum efficiency (EQE) of 6.6% and Commision Internationale de l'Eclairage (CIE) colour coordinates of (0.145, 0.068). This device performance has been the highest EQE observed in deep blue non-doped OLEDs with CIE coordinates less than (0.145, 0.068) to date.</P>