Molecular Characterization of Selected Local and Exotic Cattle Using RAPD Marker

Khatun, M. Mahfuza,Hossain, Khondoker Moazzem,Rahman, S.M. Mahbubur Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.6

In order to develop specific genetic markers and determine the genetic diversity of Bangladeshi native cattle (Pabna, Red Chittagong) and exotic breeds (Sahiwal), randomly amplified polymorphic DNA (RAPD) analysis was performed using 12 primers. Genomic DNA was extracted from 20 cattle (local and exotic) blood samples and extracted DNA was observed by gel electrophoresis. Among the random primers three were matched and found to be polymorphic. Genetic relations between cattle's were determined by RAPD polymorphisms from a total of 66.67%. Statistical analysis of the data, estimating the genetic distances between cattle and sketching the cluster trees were estimated by using MEGA 5.05 software. Comparatively highest genetic distance (0.834) was found between RCC-82 and SL-623. The lowest genetic distance (0.031) was observed between M-1222 and M-5730. The genetic diversity of Red Chittagong and Sahiwal cattle was relatively higher for a prescribed breed. Adequate diversity in performance and adaptability can be exploited from the study results for actual improvement accruing to conservation and development of indigenous cattle resources.

CHICKEN MYCOPLASMOSIS IN BANGLADESH

Biswas, H.R.,Khatun, H.,Mustafa, A.H.M.,Miah, A.H.,Hoque, M.M.,Rahman, M.L. Asian Australasian Association of Animal Productio 1993 Animal Bioscience Vol.6 No.2

A total of 4,800 chicken sera from Broiler, Layer, and Local chicken were tested to detect the presence of Mycoplasma gallisepticum (Mg) antibody by Rapid Serum Plate and Tube Agglutination Test. Positive cases recorded in this study were 945 (27%) in Broiler, and 436 (36.7%) in layer chicken sera and no. M. gallisepticum antibody could be seen in the local chicken sera. It is evident from the present findings that Mycoplasma gallisepticum infection has been prevailing in this country in improve breeds of chickens.

Evaluation of Wheat Genotypes for Salt Tolerance Based on Some Physiological Traits

Abu Hasan,Hafizur Rahman Hafiz,Nurealam Siddiqui,M. Khatun,Rabiul Islam,Abdullah -Al Mamun 한국작물학회 2015 Journal of crop science and biotechnology Vol.18 No.5

Identification of physiological markers related with salt tolerance during various vegetative and reproductive stages is crucial for evaluating wheat genotypes and improving their salt tolerance. Two salt-tolerant (Shatabdi and BAW 1135) and two salt-sensitive (BARI Gom 26 and BAW 1122) wheat genotypes of Bangladesh were grown in three salinity levels (control, 6 dS m-1, and 12 dS m-1) to observe the sensitivity of some physiological traits.. Salt-tolerant wheat genotypes maintained lower levels of leaf Na, higher levels of leaf K, and greater K/Na ratios in saline conditions than the sensitive one. Due to salt stress, flag leaf proline content was increased in salt-tolerant wheat genotypes whereas the proline level was decreased in the sensitive one compared to the control. Salt-sensitive genotypes showed a greater increment in SPAD (relative chlorophyll content) value at moderate salt stress but a greater reduction in SPAD values at high salt stress than tolerant ones. Salt-sensitive genotypes were affected more in their straw yield and finally grain yield plants-1 under saline conditions than salt-tolerant genotypes. There was a highly significant negative correlation between grain yield and Na content and also a highly significant negative correlation between grain yield and K : Na content of the wheat genotypes under saline environments. We also observed stress susceptibility index (SSI) based on grain yield plant-1 the order of tolerance was BAW 1135 > Shatabdi > BARI Gom 26 > BAW 1122 at moderate salinity level and BAW 1135 > Shatabdi > BAW 1122 > BARI Gom 26 at high salinity

Genetic diversity and geographic distribution of the <i>Bemisia tabaci</i> species complex in Bangladesh

Khatun, M.F.,Jahan, S.M.H.,Lee, Sukchan,Lee, Kyeong-Yeoll Elsevier 2018 Acta Tropica Vol.187 No.-

<P><B>Abstract</B></P> <P> <I>Bemisia tabaci</I> (Gennadius) is a species complex consisting of at least 40 cryptic species. Although the genetic diversity of <I>B. tabaci</I> has been studied in various regions, little is known about distribution in Bangladesh, which is covered by the Bengal delta, the largest delta on Earth. We conducted an extensive survey throughout the country and determined the nucleotide sequence of mitochondrial cytochrome <I>c</I> oxidase subunit 1 (COI) from 110 individuals. We then examined phylogenetic relationships. The results identified four cryptic species that expressed distinct interspecific variation but low intraspecific variation. Asia I was the most abundant, both Asia II 1 and Asia II 5 were moderately abundant, and Asia II 10 was found only in the central region. COI sequences of each cryptic species were distinctive and differentiated into many haplotypes. Our study provides important information to better understand the genetic diversity and geographic distribution of cryptic species in Bangladesh and nearby countries.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Genetic diversity of <I>Bemisia tabaci</I> was determined in Bangladesh. </LI> <LI> We identified four indigenous cryptic species but not MEAM1 and MED invasive cryptic species. </LI> <LI> Asia I was abundant, both Asia II 1 and Asia II 5 were moderate, and Asia II 10 was found only in the central region. </LI> <LI> Our study provides important information on the genetic diversity and geographic distribution of <I>B. tabaci</I> in Bangladesh. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Optimal control strategy to combat the spread of COVID-19 in absence of effective vaccine

M.H.A. Biswas,M.S. Khatun,M.A. Islam,S. Mandal,A.K. Paul,A. Ali 한국전산응용수학회 2022 Journal of applied mathematics & informatics Vol.40 No.3

Many regions of the world are now facing the second wave of boomed cases of COVID-19. This time, the second wave of this highly infectious disease (COVID-19) is becoming more devastating. To control the existing situation, more mass testing, and tracing of COVID-19 positive individuals are required. Furthermore, practicing to wear a face mask and maintenance of physical distancing are strongly recommended for everyone. Taking all these into consideration, an optimal control problem has been reformulated in terms of nonlinear ordinary differential equations in this paper. The aim of this study is to explore the control strategy of coronavirus-2 disease (COVID-19) and thus, minimize the number of symptomatic, asymptomatic and infected individuals as well as cost of the controls measures. The optimal control model has been analyzed analytically with the help of the necessary conditions of very well-known Pontryagin’s maximum principle. Numerical simulations of the optimal control problem are also performed to illustrate the results.

Oral absorption mechanism and anti-angiogenesis effect of taurocholic acid-linked heparin-docetaxel conjugates

Khatun, Z.,Nurunnabi, M.,Cho, K.J.,Byun, Y.,Bae, Y.H.,Lee, Y.k. Elsevier Science Publishers 2014 Journal of controlled release Vol.177 No.-

Oral delivery is the preferred route to deliver therapeutics via nanoparticles due to ease of administration and patient acceptance. Here, we report on the findings of the absorption pathway of taurocholic acid (TCA)-linked heparin and docetaxel (DTX) conjugate, which we refer to as HDTA. We studied the oral absorption of HDTA using a Caco-2 cell transport system and an animal model. We have also used other absorption enhancers, such as ethylene glycol tetraacetic acid (EGTA), or inhibitors, such as sodium azide, to compare the relative permeability of HDTA conjugates. In vivo comparative studies were conducted using free TCA as a pre-administration and exhibited the maximum absorption site of the organ after oral administration of HDTA conjugates. HDTA was found to be absorbed mainly in the ileum and Caco-2 cell monolayer through passive diffusion and bile acid transporters. High fluorescence intensity of HDTA in mice came from the ileum, and it was eliminated from the body through colon. This novel formulation could be further investigated by clinical trials to find the prospect of oral anti-cancer drug delivery through anti-angiogenic treatment strategies.

Sonneratia apetala (Buch.-Ham.) Fruit Extracts Ameliorate Iron Overload and Iron-Induced Oxidative Stress in Mice

Mehenaz Mithila,M Rabiul Islam,Mst Rima Khatun,M Shamim Gazi,Sheikh Julfikar Hossain 한국식품영양과학회 2023 Preventive Nutrition and Food Science Vol.28 No.3

Iron overload results in oxidative damage to various biomolecules including DNA, proteins and lipids which ultimately leads to cell death. The Sonneratia apetala fruit contains a high content of antioxidants and displays several bioactive properties. Therefore, the powder of the S. apetala fruit was successively fractionated into n-hexane (Hex), chloroform (Chl), and methanol (Met) fractions to evaluate their efficiency in ameliorating iron overload. In vitro, a colorimetric method was used to assess the Fe-chelating activity of the fractions using ferrozine. The fractions were also used in vivo to examine their efficacy in ameliorating iron overload and iron-induced oxidative stress in mice induced by intraperitoneal injection of ferric carboxymaltose at 100 mg/kg body weight (bw). Among the fractions, Met showed the highest Fe-chelation ability with an inhibitory concentration 50 of 165µg/mL followed by Hex (270 µg/mL), and Chl (418µg/mL). In vivo, the results showed a significantly (P<0.05) lower iron profile (iron and ferritin concentrations in serum and liver tissue and total iron-binding capacity of serum) in the Met and the Hex treated mice groups than in the iron-overloaded group. Met at 1,000µg/kg bw completely ameliorated iron overload in the blood and the liver tissue of mice. At this concentration, Met also prevented iron-induced oxidative stress in the liver tissue of iron-overloaded mice by restoring reducing power, total antioxidant capacity, and total protein. Thus, the S. apetala fruit, especially its Met fraction can be used in treating iron overload and associated toxicity.

Comparison of markers predicting litter size in different pig breeds

Kwon, W.-S.,Rahman, M. S.,Ryu, D.-Y.,Khatun, A.,Pang, M.-G. Wiley (Blackwell Publishing) 2017 Andrology Vol.5 No.3

<P>To overcome the limitations of conventional analysis of male fertility in animals and humans, proteomic studies have been performed to develop fertility-related biomarkers for prognosis and diagnosis of male fertility. However, the studies were focused on specific species or breeds. Therefore, a study is required to validate whether fertility-related markers would apply to other breeds in pigs. In this study, previously developed fertility-related biomarkers from Landrace were validated to use for prognosis of male fertility in commercially available breeds. Expression level of eight biomarkers in non-capacitated and capacitated (C) spermatozoa from Yorkshire and Duroc boars was analyzed. And then, to explore the validity of these markers for prognosis of male fertility, i.e. litter size, artificial insemination was performed. Among them, RAB2A (NC) and UQCRC1 (NC) turned out to be highest efficient markers for Yorkshire. RAB2A (C) was most efficient marker for Duroc. Average litter size has increased as much as 1.41 live born after prediction using eight fertility-related biomarkers in Yorkshire. In addition, average 2.52 litter size was increased after prediction using eight fertility-related biomarkers in Duroc. Average litter sizes were especially highly increased after prediction of fertility using RAB2A (NC) in Yorkshire (1.57 piglets) and TPI (NC) in Duroc (3.14 piglets), respectively. As a result, all biomarkers were significantly correlated with litter size. However, overall accuracy to predict litter size in three breeds was different in response with each marker. Average litter size after artificial insemination was also significantly affected by marker selection. Therefore, this study suggests that developed fertility-related markers may be used for prognosis and diagnosis of male fertility irrespective of breed. However, selection of efficient markers for breeds should be considered to obtain more accurate and efficient outcomes.</P>

Effect of polymorphisms in the GBP1, Mx1 and CD163 genes on host responses to PRRSV infection in pigs

Niu, P.,Shabir, N.,Khatun, A.,Seo, B.J.,Gu, S.,Lee, S.M.,Lim, S.K.,Kim, K.S.,Kim, W.I. Elsevier Scientific Pub. Co 2016 Veterinary microbiology Vol.182 No.-

<P>Porcine reproductive and respiratory syndrome (PRRS) is the most economically important disease to the swine industry, and effective prevention strategy for this disease is still required. Guanylate-binding protein 1 (GBP1) and myxovirus resistance protein 1 (Mx1) are two important proteins belonging to the GTPase superfamily that have been previously described to show antiviral effects. CD163 is considered the most important receptor for PRRSV attachment and internalization. Therefore, the aim of the present study was to evaluate the effects of these genes on host resistance against PRRSV infection in conjunction with the host immune response following PRRSV challenge. The results showed that pigs with AG genotype for the GBPI exon2 exhibited a significantly higher average daily weight gain (ADWG) and lower average viremia than AA or GG genotype. Furthermore, pigs harbouring the AG genotype for the GBP1 gene presented greater CD4(+)CD25(+) and CD8(+)CD25(+) T cell populations at 4 and 18 days post challenge (dpc), respectively, as compared with other genotypes whereas pigs with CC genotype for the CD163 gene displayed significantly higher nucleocapsid-specific antibody titers at 11 dpc. However, pigs with a single 11-bp deletion or insertion in the Mx1 gene did not show significant differences in either weight gain or viremia. Based on these results, we concluded that GBPI is most significantly associated with resistance against PRRSV infection and efficient T cell activation in pigs. (C) 2015 Elsevier B.V. All rights reserved.</P>

In vivo the antioxidative extract of Averrhoa carambola Linn. leaves induced apoptosis in Ehrilch ascites carcinoma by modulating p53 expression

Ayesha Siddika,Tasnim Zahan,Lipy Khatun,Md. Rowshanul Habib,Md. Abdul Aziz,A. R. M. Tareq,Md. Habibur Rahman,Md. Rezaul Karim 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.9

This study was designed to evaluate the antioxidantactivity of methanol extract of Averrhoa carambollaLinn. leaves (MELA) using DPPH˙ and ABTS˙+ free radicalscavenging assays whereas its antineoplastic effectagainst Ehrlich ascites carcinoma (EAC) was assed usingviable cell count, life span, body weight gain and hematologicalparameters of experimental mice. Results showedthat rich phenolic and flavonoid content of MELA hadmoderate dose dependent free radical scavenging activity(IC50: 62.0 lg/mL for DPPH˙ and 6.0 lg/mL for ABTS˙+). In vivo antineoplastic assay, MELA significantly(P\0.05) decreased viable cells and body weight gain,increased the survival time and restored altered hematologicalprofiles of cancer cell bearing mice. Fluorescencemicroscopic view of EAC cells derived from MELA-treatedgroup showed apoptotic characteristics and this observationwas also supported by overexpression of proapoptoticgenes coding p53 and Bax proteins in treatedcancer cells. The anti-apoptotic genes coding Bcl-2 proteinwas also absent in treated EAC cells as compared with thecontrol. Moreover, phytochemical profiles of MELA asidentified by GC/MS analysis are also consistent with itsactivities.