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Screening a Panel of Acid-producing Strains by Developing a High-throughput Method
Lijuan Zhu,Hui Zhang,Shiyuan Wang,Anqi Zhao,Lingbo Qu,Wenlong Xiong,Md. Asraful Alam,Wenlong Ma,Yongkun Lv,Jingliang Xu 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.5
Organic acids are natural cellular metabolites, which are widely used in food, pharmaceutical, and chemical industries. Among them, L-lactic acid is of special interest, because it is widely used in food and pharmaceutical industries and its monopolymer (poly (lactic acid)) is a green, renewable, biodegradable, and biocompatible alternative to the petroleum-based polymers. Currently, organic acids are predominantly produced by microbial fermentation. Their productions have been substantially improved by genetic modifications, metabolic engineering, and fermentation optimizations. However, the commonly used microbial producers still suffer from low acidic tolerance. Screening higher tolerant acid-producing microorganisms from the nature is relatively less explored. The traditional fermented foods are good resources for the screening of acid-producing and probiotic microorganisms. However, they are relatively less explored, especially those foods in developing countries. To speed up the acid-producing microorganism screening, we developed and validated a high-throughput method in this study. By using this method, we screened 1,296 colonies in 4 days and obtained a panel of acid-producing microorganisms. Among them, a Lacticaseibacillus rhamnosus showed the potential for organic acid production and probiotics applications.
송윤선,Lee Dongwhane,서대철,Joong-goo Kim,김재균,Minkyu Han,Liu Hairi,Lingbo Zhao,Eun Hye Kim,Sung Chul Jung,Dong-geun Lee,구현정,Min-ju Kim,Seunghee Baek,황선문,Bum Joon Kim,Yeon-Jung Kim,조홍준,김상준,Sang-Beom Jeon,Jon 대한신경중재치료의학회 2019 Neurointervention Vol.14 No.1
Purpose: Cigarette smoking (CS) is one of the major risk factors of cerebral atherosclerotic disease, however, its level of contribution to extracranial and intracranial atherosclerotic stenosis (ECAS and ICAS) was not fully revealed yet. The purpose of our study was to assess the association of CS to cerebral atherosclerosis along with other risk factors. Materials and Method:s All consecutive patients who were angiographically confirmed with severe symptomatic cerebral atherosclerotic disease between January 2002 and December 2012 were included in this study. Multivariate logistic regression analyses were performed to identify risk factors for ECAS and ICAS. Thereafter, CS group were compared to non-CS group in the entire study population and in a propensity-score matched population with two different age-subgroups. Results: Of 1709 enrolled patients, 794 (46.5%) had extracranial (EC) lesions and the other 915 (53.5%) had intracranial (IC) lesions. CS group had more EC lesions (55.8% vs. 35.3%, P<0.001) whereas young age group (<50 years) had more IC lesion (84.5% vs. 47.6%, P<0.001). In multivariate analysis, seven variables including CS, male, old age, coronary heart disease, higher erythrocyte sedimentation rate, multiple lesions, and anterior lesion were independently associated with ECAS. In the propensity-score matched CS group had significant more EC lesion compared to non-CS group (65.7% vs. 47.9%) only in the old age subgroup. Conclusion: In contrast to a significant association between CS and severe symptomatic ECAS shown in old population, young patients did not show this association and showed relatively higher preference of ICAS.