Methylation-associated silencing of BASP1 contributes to leukemogenesis in t(8;21) acute myeloid leukemia

Lei Zhou,Lin Fu,Na Lv,Jing Liu,Yan Li,Xiaosu Chen,Qingyu Xu,Guofeng Chen,Baoxu Pang,Lili Wang,Yonghui Li,Xiaodong Zhang,Li Yu 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

The AML1-ETO fusion protein (A/E), which results from the t(8;21) translocation, is considered to be a leukemiainitiating event. Identifying the mechanisms underlying the oncogenic activity of A/E remains a major challenge. In this study, we identified a specific down-regulation of brain acid-soluble protein 1 (BASP1) in t(8;21) acute myeloid leukemia (AML). A/E recognized AML1-binding sites and recruited DNA methyltransferase 3a (DNMT3a) to the BASP1 promoter sequence, which triggered DNA methylation-mediated silencing of BASP1. Ectopic expression of BASP1 inhibited proliferation and the colony-forming ability of A/E-positive AML cell lines and led to apoptosis and cell cycle arrest. The DNMT inhibitor decitabine up-regulated the expression of BASP1 in A/E-positive AML cell lines. In conclusion, our data suggest that BASP1 silencing via promoter methylation may be involved in A/E-mediated leukemogenesis and that BASP1 targeting may be an actionable therapeutic strategy in t(8;21) AML.

Long-term Prognosis in Hepatocellular Carcinoma Patients after Hepatectomy

Zhou, Lei,Liu, Chang,Meng, Fan-Di,Qu, Kai,Tian, Feng,Tai, Ming-Hui,Wei, Ji-Chao,Wang, Rui-Tao Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

Background: The hepatocellular carcinoma is very common in China. Our aim in this report was to investigate clinical and pathological factors based on the current decade data that could influence prognosis of HCC patients after hepatectomy. Methods: Between 2002 and 2009, all patients undergoing hepatectomy for HCC were followed up and reviewed retrospectively. Prognostic factors were studied by univariate and multivariate analysis, with Kaplan-Meier and Cox multivariate survival analyses. Results: Complete clinicopathologic and follow-up data were available for 114 patients. The estimated cumulative survival rates at 1, 3, and 5 yr were 84.6%, 60.2% and 51.8%, respectively. On univariate analysis, key prognostic factors were AFP level, GGT level, tumor size, number of tumors, portal vein invasion, liver cirrhosis status and TNM stage. In the multivariate analysis, tumor size, GGT level, liver cirrhosis status and portal vein invasion were significantly associated with patients' prognosis. Conclusion: Through follow-up of a relatively large cohort of Chinese patients, tumor size, GGT level, liver cirrhosis status, portal vein invasion were revealed as important factors for long-term survival after hepatectomy. Early diagnosis for tumor and the improvement of liver function before surgery are important ways to improve the prognosis.

〈운영전〉에서 표출된 임진왜란 후 사회계층의 신분구조 변동양상 및 작가의식 소고

( Zhou Lei ),( Fan Shujie ) 한국언어문화학회 2013 한국언어문화 Vol.0 No.51

As one of the most representative novels in Chosun Dynasty, 〈Woon-Young Jeon〉beautifies the love between men and women. Because the novel profoundly reflects the social reality at that time, it has attracted extensive attention from scholars during this period. However, scholars have focused on the research on the female characters or Anpyeong Daegun in this novel during this period. In addition to Anpyeong Daegun, the study about other male characters appearing in this novel failed to actively carry out. Especially, through the analysis of the male characters, the research on that investigate the changes in the identity structure of social stratum after the War of Im­jin as well as the consciousness of the writer being related to it is still in the primary stage. The author believes that any literary works are inseparable from the background andare bound to reflect the social reality of that times writers who lived in. Therefore, analysing of Yuyeong and Teuk, this thesis aims to investigate the changes in the identity structure of social stratum as well as the consciousness of the writer being related to it in the early 17th century.

A Low Power Consumption Frequency Adaptation Mechanism Based on the Traffic and Implementation on NetFPGA

Lei Zhou,Lianghua Li,Xiaoju Liu,Xiaojun Wang 보안공학연구지원센터 2014 International Journal of Future Generation Communi Vol.7 No.6

Both in academic and industrial research, building a green energy-saving network has become a hot topic. Combined with hardware division and adaptive algorithm, this paper proposes an adaptive frequency control mechanism based on traffic to reduce power consumption. By calculating real-time traffic, the network device can adjust its operating frequency. On the premise of maintaining network performance, this mechanism could reduce the power consumption significantly. Besides, the adaptive frequency mechanism has been verified and realized in the NetFPGA Reference Router. Experimental data show that under different ranges of traffic loads and without affect its performance, the Router embedded this mechanism can reduce power consumption by more than 20%.

Aerodynamic Characteristics of Neighboring Building Exposed to Twisted Wind

Lei Zhou,KamTim Tse,Gang Hu Council on Tall Building and Urban Habitat Korea 2022 International journal of high-rise buildings Vol.11 No.4

The conventional wind and twisted-wind effect on aerodynamic properties of neighboring buildings arranged in side-by-side and tandem systems at 2B and 5B spacings are systematically investigated by large eddy simulation. Different physical interactions between different wind profiles and neighboring buildings will be deeply understood. The neighboring-building system under two different types of wind profiles, i.e., conventional wind profile (CWP), twisted wind profiles (TWP) with the maximum twisted angle of 30°, is used to evaluate the variation of physical mechanism between wind and buildings. Aerodynamic characteristics including mean and RMS pressure coefficient, and velocity field were systematically analyzed and compared between different scenario. It was found that the distribution of mean pressure, root-mean-square x velocity and the streamline of wind flow for TWP greatly deviated from CWP, and the effect of TWP on the downstream building, was drastically different from that of CWP, such as the size of vortexes after the lower stream building being bigger when exposed to TWP, and the mean pressure distribution on the building surfaces are also different. Moreover, evidence of buildings arranged in side-by-side and tandem configurations having interchangeable properties under TWP was also discovered, that two buildings being arranged side-by-side exposed to TWP could be identified as being arranged in tandem with a different wind twist angle, or vice versa.

"Farming" in osteosarcoma

Zhou Lei,Rong Yuan,R W H PHO 가톨릭중앙의료원 가톨릭암센터 1997 암심포지움 Vol.- No.1

Osteosarcoma may be the result of malignant transformation of mesenchymal stromal cell and characterised by direct formation of osteoid or bone. They are morphologically heterogenous with histological diversity. They can therefore be classified be classified into fibroblastic (spindle cell predominant), osteoblastic (bone predominant), chondroblastic (cartilage or chondroid differentiation) or telangiectatic (vascular differentiation). This heterogenicity may reflect the various developmental stages of single cell strain or the mutation of single cell. Being cancer cell, they have escaped from normal cell cycle regulation. They invade and colonise territories with unregulated growth. They have predilection site in metaphyseal of long bone(e.g. distal femur and proximal humerus) arising de no-va as primary osteosarcoma of secondary to pre-existing conditions (Paget's disease, multiple enchondroma, bone infarct, bone irradiation, etc). They can also occur in extraskeletal tissue. The tissue that have high growth and reparative process appear to form an excellent environment or "soil" for the invasive osteosarcoma to develop with its uncontrolled growth and ultimately metastases to other organs.

Study on shear fracture behavior of soft filling in concrete specimens: Experimental tests and numerical simulation

Lei Zhou,Vahab Sarfarazi,Hadi Haeri,Amir Aslan Naderi,Mohammad Fatehi Marji,Fei Wu 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.85 No.3

In this paper, the shear behavior of soft filling in rectangular-hollow concrete specimens was simulated using the 2D particle flow code (PFC2D). The laboratory-measured properties were used to calibrate some PFC2D micro-properties for modeling the behavior of geo-materials. The dimensions of prepared and modeled samples were 100 mm×100 mm. Some disc type narrow bands were removed from the central part of the model and different lengths of bridge areas (i.e., the distance between internal tips of two joints) with lengths of 30 mm, 50 mm, and 70 mm were produced. Then, the middle of the rectangular hollow was filled with cement material. Three filling sizes with dimensions of 5 mm×5 mm, 10 mm×5 mm, and 15 mm×5 mm were provided for different modeled samples. The parallel bond model was used to calibrate and re-produce these modeled specimens. Therefore, totally, 9 different types of samples were designed for the shear tests in PFC2D. The shear load was gradually applied to the model under a constant loading condition of 3 MPa (σc/3). The loading was continued till shear failure occur in the modeled concrete specimens. It has been shown that both tensile and shear cracks may occur in the fillings. The shear cracks mainly initiated from the crack (joint) tips and coalesced with another one. The shear displacements and shear strengths were both increased as the filling dimensions increased (for the case of a bridge area with a particular fixed length).

The effect of radial cracks on tunnel stability

Zhou, Lei,Zhu, Zheming,Liu, Bang,Fan, Yong Techno-Press 2018 Geomechanics & engineering Vol.15 No.2

The surrounding rock mass contains cracks and joints which are distributed randomly around tunnels, and in the process of tunnel blasting excavation, radial cracks could also be induced in the surrounding rock mass. In order to clearly understand the impact of radial cracks on tunnel stability, tunnel model tests and finite element numerical analysis were implemented in this paper. Two kinds of materials: cement mortar and sandstone, were used to make tunnel models, which were loaded vertically and confined horizontally. The tunnel failure pattern was simulated by using RFPA2D code, and the Tresca stresses and the stress intensity factors were calculated by using ABAQUS code, which were applied to the analysis of tunnel model test results. The numerical results generally agree with the model test results, and the mode II stress intensity factors calculated by ABAQUS code can well explain the model test results. It can be seen that for tunnels with a radial crack emanating from three points on tunnel edge, i.e., the middle point between tunnel spandrel and its top with a dip angle $45^{\circ}$, the tunnel foot with a dip angle $127^{\circ}$, and the tunnel spandrel with $135^{\circ}$ with tunnel wall, the tunnel model strength is about a half of the regular tunnel model strength, and the corresponding tunnel stability decreases largely.

Transient ion ejection during nanocomposite thermite reactions

Zhou, Lei,Piekiel, Nicholas,Chowdhury, Snehaunshu,Lee, Donggeun,Zachariah, Michael R. American Institute of Physics 2009 JOURNAL OF APPLIED PHYSICS - Vol.106 No.8

PLAXIS 3D simulation, FLAC3D analysis and in situ monitoring of Excavation stability

Lei Zhou,Zahra Jalalichi,Vahab Sarfarazi,Hadi Haeri,Parviz Moarefvand,Mohammad Fatehi Marji,Shahin Fattahi 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.84 No.6

Near-surface excavations may cause the tilting and destruction of the adjacent superstructures in big cities. The stability of a huge excavation and its nearby superstructures was studied in this paper. Some test instruments monitored the deformation and loads at the designed location. Then the numerical models of the excavation were made in FLAC3D (a threedimensional finite difference code) and Plaxis-3D (a three-dimensional finite element code). The effects of different supporting and reinforcement tools such as nails, piles, and shotcretes on the stability and bearing capacity of the foundation were analyzed through different numerical models. The numerically approximated results were compared with the corresponding in-field monitored results and reasonable compatibility was obtained. It was concluded that the displacement in excavation and the settlement of the nearby superstructure increases gradually as the depth of excavation rises. The effects of support and reinforcements were also observed and modeled in this study. The settlement of the structure gradually decreased as the supports were installed. These analyses showed that the pile significantly increased the bearing capacity and decreased the settlement of the superstructure. As a whole, the monitoring and numerical simulation results were in good consistency with one another in this practically important project.