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Effect of sodium silicate on early growth stages of wheat under drought stress
Lee Sang Gyu,Lee Hyeri,Lee Byung Cheon,Lee Hojoung,Moon Jun Cheol,Choi Changhyun,Chung Namhyun 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.4
Wheat yield is decreasing due to climate change, and a method to prevent decreasing yield during drought stress is desirable. In this study, wheat cultivars (Koso and Jokyung) were treated with 15% polyethylene glycol-6000 (PEG) and PEG + Si solution (6.5, 8.7, 13.1 and 26.1 mM). The effect of Si treatment on the alleviation of drought stress was measured using the germination test, shoot relative water content (RWC), seedling stage observation, and quantitative real time polymerase chain reaction (qRT-PCR). The results of root/shoot length ratio and shoot length ratio showed that Si treatment induced the alleviation of drought stress in Jokyung cultivar. The result of qRT-PCR showed the alleviation of drought stress in Koso cultivar. In addition, the results with shoot RWC and seedling stage observation showed that the alleviation effects of Si treatment was observed with both Koso and Jokyung cultivar at the high concentration of Si (26.1 mM). All these results suggest that Si treatment at a high concentration could be employed to alleviate drought stress in wheat.
Lee, Hyeri,In, Byunggyu,Mehta, Pramod Kumar,Kishore, Mallela Y. L. N.,Lee, Keun-Hyeung American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.3
<P>The detection of fluorescent probes for biomolecules and control of the function of a complex through a recognition process have not been investigated intensively. A fluorescent peptidyl probe (1) based on the self-assembly stimulated by heparin was synthesized. The fluorescent probe with an aggregation-induced emission fluorophore formed a self assembling complex with heparin, resulting in a sensitive and selective turn-on response to heparin compared to its biological competitors. The detection limits for heparin were measured to be 138.0 pM (R-2 = 0.976) in aqueous solution and 2.6 nM (R-2 = 0.996) in aqueous solution containing human serum. Nanosized aggregates formed through the self-assembly of the complex showed potent resistance against the heparin-digestive enzyme. The dual role of the probe for the detection of heparin and the inhibition of heparinase-mediated digestion through the recognition process was used for the real-time monitoring of the enzyme activity of heparinase for the digestion of heparin. Furthermore, the dual role of the probe was applied for the detection of the oversulfated chondroitin sulfate contaminant in heparin.</P>
Lee, Hyeri,Riu, Myoungjoo,Kim, Eunhye,Moon, Joon-Kwan,Choi, Hoon,Do, Jung-A,Oh, Jae-Ho,Kwon, Ki-Sung,Lee, Young Deuk,Kim, Jeong-Han 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.2
A single residue analytical method was developed for herbicide chlorpropham in various crops. Brown rice, apple, mandarin, Kimchi cabbage, green pepper, potato, and soybean were selected as representative crops, and clean-up system, partition solvent, and extraction solvent were optimized. For high performance liquid chromatography (HPLC), $C_{18}$ column was used with elution solvents of water and acetonitrile. Limit of quantitation (LOQ) of chlorpropham was 2 ng (S/N >20), and excellent linearity ($R^2$=1.000) was achieved. Method limit of quantitation (MLOQ) was 0.02 mg/kg. For recovery tests, crop samples were macerated and fortified with chlorpropham standard solution at three fortification levels (MLOQ, 10 MLOQ, and 100 MLOQ). And then those were extracted with acetonitrile, concentrated and partitioned with n-hexane. The n-hexane layer was then concentrated, cleaned-up through Florisil$^{(R)}$ column with ethyl acetate:n-hexane (5:95, v/v) prior to concentration and analysis with HPLC. Good recoveries from 76.8 to 107.9% with coefficients of variation of less than 10% were obtained, regardless of sample type, which satisfies the criteria of Korea Food and Drug Administration. Those results were confirmed with liquid chromatography-mass spectrometry (LC-MS). The method established in this study could be applied to most of crops as an official and general method for the analysis of chlorpropham residue.
Lee, Hyeri,Park, Tae-Hyeon,Jang, Du-Jeon The Royal Society of Chemistry 2016 NEW JOURNAL OF CHEMISTRY Vol.40 No.10
<P>Well-defined uniform anatase TiO2 nanotube (TNT) arrays with predominant exposure of the highly energetic {001} facets have been prepared via a two-step anodic oxidation process of Ti foil. In order to enhance the exposure degree of the {001} facets, NH4F has been used as a capping agent during anodization and the evolution of TNT arrays has been varied by changing the duration of anodization and the concentration of NH4F. With the increase of the first-anodization time (up to 11 h) and/or the NH4F concentration, the crystallite sizes, crystallinity, and exposed {001} facets of prepared TNT arrays have been found to increase. The photocatalytic activity of the as-prepared TNT arrays, evaluated by monitoring the photodegradation of rhodamine B, has been found to increase in accordance with an increase in the exposure degree of the {001} facets, indicating that the highly energetic {001} facets enhance the photocatalytic activity of TNT arrays on a large scale. The {001} facets of TNT arrays have been considered to provide oxidation sites extensively, which produce active oxygen species such as (OH)-O-center dot, O-center dot(2)-, and H2O2 upon UV irradiation. Our results have shown that similar to OH is the predominant highly active species for the photocatalytic degradation of organic dye pollutants via TNT arrays. We consider that this study may provide a new insight into the preparation and development of advanced photocatalytic anatase TiO2 materials.</P>