Effects of L-malic acid on alpha-glucosidase: inhibition kinetics and computational molecular dynamics simulations.

Gou, Lin,Zhan, Yi,Lee, Jinhyuk,Li, Xuan,Lü,, Zhi-Rong,Zhou, Hai-Meng,Lu, Hang,Wang, Xi-Yao,Park, Yong-Doo,Yang, Jun-Mo Humana Press 2015 Applied biochemistry and biotechnology Vol.175 No.4

<P>The inhibitory effect of L-malic acid (MA) on alpha-glucosidase (EC 3.2.1.20) was investigated by combination study between inhibition kinetics and computational simulations. The results from the serial kinetics demonstrated that MA could directly inactivate the enzyme activity in a dose-dependent manner and a typical non-competitive type, as well as in a fast inactivate process without detectable time course. The tertiary conformation study with an application of spectrofluorimetry showed that MA modulated the tertiary structural conformation of alpha-glucosidase both on the overall and on regional active site pocket, which monitored by red-shift intrinsic fluorescence peak with decreases intensities, and the significant intensity increasing of 1-anilinonaphthalene-8-sulfonate (ANS)-binding fluorescence, respectively. To have more insight, we also adapted the computational molecular dynamics (MD) simulations. The results showed that MA was located in the entrance of active pocket for the catalytic reaction and blocked the passage of substrate. It confirmed that MA inhibits as a non-competitive type, not direct docking to the glucose binding site. Our study provides important molecular mechanisms to figure out alpha-glucosidase inhibition that might associate to development of type 2 diabetes mellitus drug.</P>

Complex molecules in the W51 North region

Rong, J.,Qin, S.-L.,Zapata, L. A.,Wu, Y.,Liu, T.,Zhang, C.,Peng, Y.,Zhang, L.,Liu, Y. Oxford University Press 2016 MONTHLY NOTICES- ROYAL ASTRONOMICAL SOCIETY Vol.455 No.2

Study in Hydrogen Ion Irradiation of N+-Ion-Implanted SiC-C Films

L. Jia-Rong,N. K. Huang 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.46 No.1

70 % SiC-C ¯lms were deposited by r.f. magnetron sputtering on stainless steel or NaCl substrates, followed by argon ion beam bombardment or nitrogen ion implantation. These 70 % SiC-C ¯lms were irradiated with a hydrogen-ion beam at 5 keV and a dose of 1£1018 ions/cm2. Microanalyses of secondary-ion mass spectroscopy, Raman spectroscopy and X-ray photoelectron spectroscopy were used to study hydrogen behaviors in these 70 % SiC-C ¯lms, in order to study the eect of nitrogen on hydrogen behaviors of the carbon-carbide ¯lms. It is found that hydrogen concentration in the nitrogen ion implanted 70 % SiC-C ¯lms is higher than that in the argon ion bombarded 70 % SiC-C ¯lms. Related mechanism is discussed in this paper.

MODEL PREDICTIVE CONTROL OF NONLINEAR PROCESSES BY USE OF 2ND AND 3RD VOLTERRA KERNEL MODEL

H. Kashiwagi,L. RONG,H. Harada,T. Yamaguchi 제어로봇시스템학회 1998 제어로봇시스템학회 국내학술대회 논문집 Vol.1998 No.10

Trifluoroethanol-induced changes in activity and conformation of manganese-containing superoxide dismutase.

Yin, Shang-Jun,L?, Zhi-Rong,Park, Daeui,Chung, Hae Young,Yang, Jun-Mo,Zhou, Hai-Meng,Qian, Guo-Ying,Park, Yong-Doo Humana Press 2012 Applied biochemistry and biotechnology Vol.166 No.2

<P>Superoxide dismutase (SOD, EC 1.15.1.1) plays an important role in antioxidant defense in organisms exposed to oxygen. However, there is a lack of research into the regulation of SOD activity and structural changes during folding, especially for SOD originating from extremophiles. We studied the inhibitory effects of trifluoroethanol (TFE) on the activity and conformation of manganese-containing SOD (Mn-SOD) from Thermus thermophilus. TFE decreased the degree of secondary structure of Mn-SOD, which directly resulted in enzyme inactivation and disrupted the tertiary structure of Mn-SOD. The kinetic studies showed that TFE-induced inactivation of Mn-SOD is a first-order reaction and that the regional Mn-contained active site is very stable compared to the overall structure. We further simulated the docking between Mn-SOD and TFE (binding energy for Dock 6.3, -9.68 kcal/mol) and predicted that the LEU9, TYR13, and HIS29 residues outside of the active site interact with TFE. Our results provide insight into the inactivation of Mn-SOD during unfolding in the presence of TFE and allow us to describe ligand binding via inhibition kinetics combined with computational predictions.</P>

Effect of Deformation on the Stress-Induced Martensitic Transformation in (Ni(47) Ti(44))(100-x)Nbx Shape Memory Alloys with Wide Hysteresis

( X. M. He ),( L. J. Rong ) 대한금속재료학회 ( 구 대한금속학회 ) 2006 METALS AND MATERIALS International Vol.12 No.4

Martensite in TiNi-based alloys is reported to be thermally stabilized after a moderate deformation. Hence, this paper investigates the effect of deformation via stress-induced martensitic transformation on the reverse transformation behavior of (Ni(47)Ti(44))(100-x)Nbx(x=3, 9, 15, 20, 30 at.%) alloys. The stress-induced martensite appears to be stabilized in relation to the thermal-induced martensite that forms on cooling. This observation is confirmed by an increase in the reverse transformation start temperature, during which time the transformation temperature hysteresis reaches about 200℃. Moreover, the Nb content in Ni-Ti-Nb alloy has a great influence on the transformation temperature hysteresis of stress-induced martensite as well as on the process of stress-induced martensitic transformation. The mechanism of wide transformation temperature hysteresis is explained in terms of the microscopic structure of (Ni(47)Ti(44))(100-x)Nbx alloys. Furthermore, the temperature interval of the reverse transformation of stress-induced martensite was found to increase slightly as the strain of the high Nb-content alloy increased, though the value was much smaller than that of the thermally induced martensite. Finally, the paper explains the relation between this unique phenomenon and the elastic strain energy.

Towards profiling the gene expression of tyrosinase-induced melanogenesis in HEK293 cells: a functional DNA chip microarray and interactomics studies.

Cho, Ick-Hyun,Lü,, Zhi-Rong,Yu, Jae-Ran,Park, Yong-Doo,Yang, Jun-Mo,Hahn, Myong-Joon,Zou, Fei Adenine Press 2009 Journal of biomolecular structure & dynamics Vol.27 No.3

<P>The overexpression of a single tyrosinase gene can induce conspicuous pigmentation in nonpigmented cells. We hypothesized that some unknown tyrosinase-associated genes are simultaneously regulated by melanin synthesis. To improve understanding of melanogenesis and tyrosinase-associated functions, we attempted to profile the genes that are altered during melanin production in HEK293 cells by using a functional DNA chip microarray. The candidate genes were obtained based on significance analysis of microarray (SAM) and further computational prediction via protein-protein interaction (PPI) mapping suggested that newly detected hub genes were involved in melanogenesis. PPI mapping using bioinformatic tools revealed 8 genes that formed an interaction hub. The yeast two-hybridization results suggested some candidate genes could interact with tyrosinase. The present study provides information to further understand the complex factors associated with tyrosinase-induced melanogenesis and apoptosis. The approach of combining expression data analysis and predicted protein interaction partners can help identify genes involved in pigmentation.</P>

Concrete structural health monitoring using piezoceramic-based wireless sensor networks

Li, Peng,Gu, Haichang,Song, Gangbing,Zheng, Rong,Mo, Y.L. Techno-Press 2010 Smart Structures and Systems, An International Jou Vol.6 No.5

Impact detection and health monitoring are very important tasks for civil infrastructures, such as bridges. Piezoceramic based transducers are widely researched for these tasks due to the piezoceramic material's inherent advantages of dual sensing and actuation ability, which enables the active sensing method for structural health monitoring with a network of piezoceramic transducers. Wireless sensor networks, which are easy for deployment, have great potential in health monitoring systems for large civil infrastructures to identify early-age damages. However, most commercial wireless sensor networks are general purpose and may not be optimized for a network of piezoceramic based transducers. Wireless networks of piezoceramic transducers for active sensing have special requirements, such as relatively high sampling rate (at a few-thousand Hz), incorporation of an amplifier for the piezoceramic element for actuation, and low energy consumption for actuation. In this paper, a wireless network is specially designed for piezoceramic transducers to implement impact detection and active sensing for structural health monitoring. A power efficient embedded system is designed to form the wireless sensor network that is capable of high sampling rate. A 32 bit RISC wireless microcontroller is chosen as the main processor. Detailed design of the hardware system and software system of the wireless sensor network is presented in this paper. To verify the functionality of the wireless sensor network, it is deployed on a two-story concrete frame with embedded piezoceramic transducers, and the active sensing property of piezoceramic material is used to detect the damage in the structure. Experimental results show that the wireless sensor network can effectively implement active sensing and impact detection with high sampling rate while maintaining low power consumption by performing offline data processing and minimizing wireless communication.

Incidence of Thymoma in Myasthenia Gravis: A Systematic Review

Zhi-Feng Mao,Xue-An Mo,Yong-Rong Lai,Maree L. Hackett 대한신경과학회 2012 Journal of Clinical Neurology Vol.8 No.3

Background and Purpose Myasthenia gravis (MG) is usually comorbid with thymoma. More accurate estimates of the incidence thymoma in MG will help inform patients and their physicians, facilitate health policy discussions, provide etiologic clues, and optimize the management of MG. Methods We conducted a systematic review search of relevant English-language studies published between 1960 and 2012 using MEDLINE and Embase. We identified additional studies by reviewing the bibliographies of the retrieved articles and hand searched the main neurology journals. Only incidence studies and case series of unselected MG patients in which information about thymoma were included. Results Out of 2206 potentially relevant studies, 49 met the inclusion criteria. Although there was a considerable degree of heterogeneity, the pooled estimate of the incidence of thymoma in MG was 21% (95% confidence interval, 20-22%). The pooled incidence was significantly higher for surgery-based studies than for population- and hospital-based studies. A large proportion of the reported thymomas were noninvasive. Furthermore, thymoma appears to occur significantly more frequently among male MG patients and those older than 40 years at the onset of MG. Conclusions Thymoma is common in MG patients, but appears to be found more often in male MG patients and those older than 40 years at the onset of MG. Further research is needed to expand our understanding of these association conditions

<i>Premnagrandipaniculata</i> ( Lamiaceae , Premnoideae ), a remarkable new species from north Myanmar

Tan, Yun-Hong,Li, De-Rong,Zhou, Shi-Shun,Chen, Yong-Jun,Bramley, Gemma L.C.,Li, Bo Pensoft Publishers 2018 PhytoKeys Vol.94 No.-

<P>Abstract</P><P>A remarkable new <I>Premna</I> species from Myanmar, <I>P.grandipaniculata</I> Y.H.Tan & Bo Li (Lamiaceae), is here described and illustrated. It differs from all known congeneric taxa by having huge complicated panicles which have tertiary branches formed by spike-like thyrses. In <I>Premna</I>, such a spike-like thyrse is found in <I>P.bracteata</I> and <I>P.interrupta</I>, but those species can be easily distinguished from <I>P.grandipaniculata</I> by their habit, indumentum, leaf size and inflorescence structure.</P>