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      • KCI등재

        Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology

        Kui Wu,Yangyun Zheng,Qingping Wu,Haiying Chen,Songzhe Fu,Biao Kan,Yongyan Long,Xiansheng Ni,Junling Tu 한국미생물학회 2019 The journal of microbiology Vol.57 No.12

        In order to adapt to different environments, Vibrio parahaemolyticus employed a complicated quorum sensing system to orchestrate gene expression and diverse colony morphology patterns. In this study, the function of the putative quorum sensing signal synthase gene cqsA (VPA0711 in V. parahaemolyticus strain RIMD2210633 genome) was investigated. The cloning and expression of V. parahaemolyticus cqsA in Escherichia coli system induced the production of a new quorum sensing signal that was found in its culture supernatant. The signal was purified by high performance liquid chromatography methods and determined to be 3-hydroxyundecan- 4-one by indirect and direct mass spectra assays. The deletion of cqsA in RIMD2210633 changed V. parahaemolyticus colony morphology from the classical ‘fried-egg’ shape (thick and opaque in the center, while thin and translucent in the edge) of the wild-type colony to a ‘pancake’ shape (no significant difference between the centre and the edge) of the cqsAdeleted colony. This morphological change could be restored by complementary experiment with cqsA gene or the signal extract. In addition, the expression of opaR, a well-known quorum sensing regulatory gene, could be up-regulated by cqsA deletion. Our results suggested that V. parahaemolyticus used cqsA to produce 3-hydroxyundecan-4-one signal and thereby regulated colony morphology and other quorum sensing-associated behaviors.

      • KCI등재

        Effect of Concentrations of Fe2+ and Fe3+ on the Corrosion Behavior of Carbon Steel in Cl− and SO42− Aqueous Environments

        Kui Xiao,Zhaoliang Li,Jialiang Song,Ziheng Bai,Wei Xue,Junsheng Wu,Chaofang Dong 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.8

        The concentration of metal ions in aqueous environments signifcantly afects the formation of corrosion products and furthermetal corrosion. In this paper, the electrochemical behavior of carbon steel in the presence of Fe2+ or Fe3+ concentrationin Cl− and SO42− aqueous environments have been investigated using conventional electrochemical methods such as linearpolarization and alternating current impedance spectroscopy. The morphology and composition of carbon steel corrosionproducts were studied using scanning electron microscopy and laser Raman spectroscopy. The efects of corrosion productsand iron ions concentration on the corrosion of carbon steel were discussed. Corrosion products of carbon steel in aqueousenvironments were γ-FeOOH, γ-Fe2O3 and a small amount of α-Fe2O3. The addition of Fe2+ afected the cathode reactionof the electrode reaction, and promotes the formation of γ-FeOOH and Fe3O4. And with the increase of Fe2+ concentrationin the solution, the anode process of electrode reaction was suppressed. The addition of Fe3+ promoted the formation ofγ-Fe2O3 and Fe3O4. Fe3+ afected the ionization of water, causing the pH of the solution to drop. Fe3+ undergoes a redoxreaction with the matrix. Addition of Fe3+ ions promoted the formation of FeOOH, an intermediate product, which reactswith the anode product Fe2+. These factors all accelerated the corrosion process.

      • SCIESCOPUSKCI등재

        Use of Long-chain Alcohol in Extraction and Purification of Lincomycin from Fermentation Broth

        Wu, Bin,Zhu, Jiawen,Chen, Kui,Ji, Lijun,Guo, Jianguo,Zhao, Jianxi 한국화학공학회 2003 Korean Journal of Chemical Engineering Vol.20 No.2

        Low selectivity for lincomycin in butanol extraction process leads to relatively higher content of impurities. A novel process for extraction of lincomycin from fermentation broth was studied in this work. Mixture of n-octanol and n-decanol is used as extractant to replace n-butanol in extraction of lincomycin. Oprimal operation conditions for the process have been studied. Due to higher extraction selectivity for lincomycin A by long-chain alcohol, content of impurity(lincomycin B) in the final product is much lower than that in product by butanol process. Furthemore, the practicability for combination of long-chain alcohol and butanol in purification of linecomycin was investigated.

      • Fluorine-Substituted Dithienylbenzodiimide-Based n-Type Polymer Semiconductors for Organic Thin-Film Transistors

        Feng, Kui,Zhang, Xianhe,Wu, Ziang,Shi, Yongqiang,Su, Mengyao,Yang, Kun,Wang, Yang,Sun, Huiliang,Min, Jie,Zhang, Yujie,Cheng, Xing,Woo, Han Young,Guo, Xugang American Chemical Society 2019 ACS APPLIED MATERIALS & INTERFACES Vol.11 No.39

        <P>Imide functionalization is one of the most effective approaches to develop electron-deficient building blocks for constructing n-type organic semiconductors. Driven by the attractive properties of imide-functionalized dithienylbenzodiimide (TBDI) and the promising device performance of TBDI-based polymers, a novel acceptor with increased electron affinity, fluorinated dithienylbenzodiimide (TFBDI), was designed with the hydrogen replaced by fluorine on the benzene core, and the synthetic challenges associated with this highly electron-deficient fluorinated imide building block are successfully overcome. TFBDI showed suppressed frontier molecular orbital energy levels as compared with TBDI. Copolymerizing this new electron-withdrawing TBDI with various donor co-units afforded a series of n-type polymer semiconductors TFBDI-T, TFBDI-Se, and TFBDI-BSe. All these TFBDI-based polymers exhibited a lower-lying lowest unoccupied molecular orbital (LUMO) energy level than the polymer analogue without fluorine. When applied in organic thin-film transistors, three polymers showed unipolar electron transport with large on-current/off-current ratios (<I>I</I><SUB>on</SUB>/<I>I</I><SUB>off</SUB>) of 10<SUP>5</SUP>-10<SUP>7</SUP>. Among them, the selenophene-based polymer TFBDI-Se with the deepest-positioned LUMO and optimal chain stacking exhibited the highest electron mobility of 0.30 cm<SUP>2</SUP> V<SUP>-1</SUP> s<SUP>-1</SUP>. This result demonstrates that the new TFBDI is a highly attractive electron-deficient unit for enabling n-type polymer semiconductors, and the fluorination of imide-functionalized arenes offers an effective approach to develop more electron-deficient building blocks in organic electronics.</P> [FIG OMISSION]</BR>

      • SCIESCOPUSKCI등재
      • KCI등재

        Association between Retinal Vascular Geometric Changes and Cognitive Impairment: A Systematic Review and Meta-Analysis

        Huiqun Wu,Chendong Wang,Cong Chen,Xiaotao Xu,Yi Zhu,Aimin Sang,Kui Jiang,Jiancheng Dong 대한신경과학회 2020 Journal of Clinical Neurology Vol.16 No.1

        Background and Purpose Previous studies have explored the association between retinal vascular changes and cognitive impairment. The retinal vasculature shares some characteristics with the cerebral vasculature, and quantitative changes in it could indicate cognitive impairment. Hence, a comprehensive meta-analysis was performed to clarify the potential relationship between retinal vascular geometric changes and cognitive impairment. Methods Relevant databases were scrupulously and systematically searched for retinal vascular geometric changes including caliber, tortuosity, and fractal dimension (FD), and for cognitive impairment. The Newcastle-Ottawa Scale was used to evaluate the methodological quality of included studies. RevMan was used to perform the meta-analysis and detect publication bias. Sensitivity analyses were also performed. Results Five studies that involved 2,343 subjects were finally included in the meta-analysis. The results showed that there was no significant association between central retinal artery equivalents (Z=1.17) or central retinal venular equivalents (Z=1.74) and cognitive impairment (both p>0.05). Similarly, no significant difference was detected in retinal arteriolar tortuosity (Z=0.91) and venular tortuosity (Z=1.31) (both p>0.05). However, the retinal arteriolar FD (mean difference: -0.03, 95% CI: -0.05, -0.01) and venular FD (mean difference: -0.03, 95% CI: -0.05, -0.02) were associated with cognitive impairment. Conclusions A smaller retinal microvascular FD might be associated with cognitive impairment. Further large-sample and well-controlled original studies are required to confirm the present findings.

      • KCI등재

        Screen-printed Dual-Particle-Containing Multicomponent-Blending Carbon Nanotube Cathode: Enhancement of Electron Emission Characteristics, Current Emission Stability, and Uniformity

        Yu-kui Li,Wen-xiu Wang,Chao Wu,Juan Yang 한국전기전자재료학회 2022 Transactions on Electrical and Electronic Material Vol.23 No.1

        The electron emission characteristics, current emission stability, and uniformity of screen-printed carbon nanotube were enhanced by introducing a novel dual-particle-containing multicomponent-blending carbon nanotube cathode via the comprehensive combination of baking in the air and sintering in the inert gas atmosphere. The dual-particle-containing multicomponent-blending carbon nanotube cathode’s design and manufacturing improvements ensured its steeper emission curve variation amplitude, larger maximum emission current, better electron emission current of 806.7 μA was only 2.56 V/μm. When it was increased from 2.78 to 2.91 V/μm, the enhancing current emission amplitude of the proposed cathode was increased by 600.7 μA versus 324.1 μA of the conventional non-blending carbon nanotube cathode. The maximum emission current of the proposed cathode was 3762.5 μA. After the vacant static state of 72 h, the measured emission current had no obvious attenuation, and the fl uctuation rate of emission current did not exceed 5%. The backlight unit with dualparticle-containing multicomponent-blending carbon nanotube cathode was sealed, and the obtained emission image had high luminescence brightness and uniformity.

      • SHE-PWM Control for A Four-level Hybrid-Clamped Inverter

        Mingzhe Wu,Kui Wang,Kehu Yang,Chengran Ma,Yongdong Li 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

        In this paper, a control strategy for a four-level hybrid-clamped inverter using SHE-PWM is introduced. An algebraic method combining Groebner bases and symmetric polynomials is implemented to obtain the seven switching angles of SHE-PWM. Compared with the widely-used numerical and intelligent methods, it does not need to choose initial values and is able to obtain all the solutions. Moreover, the calculation efficiency is much higher than the existing algebraic methods. The voltage balancing of the DC-link capacitors and flying capacitors can be achieved by selecting proper redundant switching states. Simulation results are presented to confirm the harmonic elimination effect and the validity of this voltage control strategy.

      • SCIESCOPUSKCI등재

        Minimum Energy Cooperative Path Routing in All-Wireless Networks: NP-Completeness and Heuristic Algorithms

        Li, Fulu,Wu, Kui,Lippman, Andrew The Korea Institute of Information and Commucation 2008 Journal of communications and networks Vol.10 No.2

        We study the routing problem in all-wireless networks based on cooperative transmissions. We model the minimum-energy cooperative path (MECP) problem and prove that this problem is NP-complete. We hence design an approximation algorithm called cooperative shortest path (CSP) algorithm that uses Dijkstra's algorithm as the basic building block and utilizes cooperative transmissions in the relaxation procedure. Compared with traditional non-cooperative shortest path algorithms, the CSP algorithm can achieve a higher energy saving and better balanced energy consumption among network nodes, especially when the network is in large scale. The nice features lead to a unique, scalable routing scheme that changes the high network density from the curse of congestion to the blessing of cooperative transmissions.

      • KCI등재

        Experimental study and modeling of residence time distribution in impinging stream reactor with GDB model

        Lijun Ji,Kui Chen,Jiawen Zhu,Bin Wu 한국공업화학회 2010 Journal of Industrial and Engineering Chemistry Vol.16 No.4

        Residence time distribution (RTD) in an impinging streams reactor with two or four nozzles was investigated with KCl solution as a tracer. The results showed that the flow pattern in the reactor was close to that in a continuous stirred tank reactor (CSTR). Macromixing process in the reactor was improved obviously when the opposite nozzles were added. Based on the analysis of flow region in the reactor, gamma distributionmodel with bypass (GDB) was applied for study on the RTD of the reactor. It was found that RTD in the impinging streams reactor could be finely described by the model. Also the effects of experimental conditions on parameters of model were analyzed according to the correlated values of the model parameters.

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