Piplartine과 전립선암 세포사멸

공은희,김광연,유선녕,이선이,안순철 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Background: Prostate cancer is one of the leading causes of cancer-related deaths in men worldwide.Piper species have known anti-proliferation and anti-cancer activity. This study was to examine the anti-proliferation effects of piplartine on human prostate cancer cell. Methods: PC-3 human prostate cancer cells received piplartine 15 uM for 24 hours. Microarray was performed on piplartine-treated PC-3 cells. Results: Among 253 genes which were altered by piplartine-treated PC-3 cells (>3-4 folds), 143 genes were up-regulated and 110 genes were down-regulated by piplartine in microarray. Most of genes whichwere up-regulated by piplartine were functionally classified as apoptosis, immune response related genes. Genes which were down-regulated by piplartine were functionally classified as transcription, cell cycle related genes. Conclusion: Piplartine may induce anti-tumor activity for human prostate cancer along with regulated of cell cycle and apoptotic genes in piplartine-induced PC-cells.

성인 여성에서 비알코올성 지방간의 중증도에 따른 대사증후군 관련성

공은희 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background: The aim of this study was to elucidate the relationship between the grade of non-alcoholic fatty liver diseases diagnosed by ultrasonography and the metabolic syndrome defined by the Third Report of the National Cholesterol Education Program Adult Treatment Panel III (NCEP-ATP III) guideline. Methods: A total of 198 women who visited a health promotion center from January, 2007 to December, 2008 were selected for this study. The ultrasound scan of the liver was performed to determine the presence and the severity of non-alcoholic fatty liver disease. The relationship between non-alcoholic fatty liver and risk categories of metabolic syndrome using NCEP-ATP III guideline were analysed. Results: The prevalence of metabolic syndrome was 19.2% in women with non-alcoholic fatty livers. When odds ratios(95% CI) for metabolic syndrome in groups with moderate and severe non-alcoholic fatty livers were compared to that of group with mild non-alcoholic fatty livers, there was an increased risk of metabolic syndrome with an odds ratio of 2.24(1.31∼4.41). Conclusion: Non-alcoholic fatty liver disease was closely related to the metabolic syndrome in healthy women and the severity classification of non-alcoholic fatty liver by ultrasound scan could be useful to predict of the severity of insulin resistance and the risk of metabolic syndrome

건강한 성인 남녀에서 폐기능과 고감도 C-반응성단백질 관련성

공은희 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background: The aim of this study was to evaluate the association of high sensitive C-reactive protein(hs-CRP) with pulmonary function as forced expiratory volume in 1 second(FEV1) within the normal range of predicted FEV1(%). Methods: The data was analyzed in healthy adult, who had visited a health promotion center at a university hospital from January, 2007 to July, 2009. Elevated hs-CRP(≥2.2 mg/L) level in relation to the values of predicted FEV1(%) as pulmonary function was studied by multiple logistic regression analysis. Results: In this cross-sectional study, there was an inverse association between hs-CRP levels and quartiles of predicted FEV1(%) in healthy adults.(P for trend=0.02 in men, P for trend<0.001 in women) The odds ratios of having elevated hs-CRP(≥2.2 mg/L) across predicted FEV1(%) quartiles, with the highest quartile(Q1) as the basal group, was 3.8(95% CI, 1.1∼3.1) in men, 4.3(95% CI, 1.1∼15.1) in women after adjusting for the confounding variables. Conclusion: The decline of predicted FEV1(%) within the normal range was inversely associated with low grade inflammation as measured by hs-CRP in healthy adults. This result showed that systemic inflammation may be linked to early impaired pulmonary function in healthy adults.

고혈압 환자와 정상인의 고혈압 인지도 비교

공은희,최종순 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

Background: Hypertension is one of the most important risk factors of cardiovascular disease and coronary artery disease. Awareness of hypertension is important in controlling of hypertension. We conducted a study on the awareness of hypertension comparing hypertensive patients with non-hypertensive patients. Methods: This study involved the number of hypertensive patients was 64, and that of non hypertensive patients was 53, who visited an outpatient of primary care practice, from November 1, to November 30, 2005. All subjects completed a questionnaire including demographic characteristics and awareness of hypertension. Results: No significant correlations were revealed between various characteristics and number of correct answers. The rate of correct answers to the questions concerning ‘frequency of blood pressure measurement' and 'treatment in asymptomatic patient' and 'life-long controlled disease' were higher in non-hypertensive patients than hypertensive patients. It was significant difference(p<0.05). The rate of correct answers to the questions concerning 'complication of hypertension' were higher in hypertensive patients than non-hypertensive patients. It was significant difference(p<0.05). All hypertensive patients correctly answered question concerning 'lifestyle modification' and 'regularly blood pressure check' and 'control of stress'. Conclusion: There was no significant difference of awareness regarding hypertension between these two groups. In order to correct wrong awareness of hypertension, We suggests the necessity of developing special program to achieve better compliance of hypertensive patients.

성인 여성에서 혈중 Gamma-glutamyltransferase(γ-GT)와 고혈압 관련성

공은희 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background: The aim of this study was to evaluate the effect of serum gamma- glutamyltransferase(γ-GT) on hypertension in healthy women. Methods: A total of 495 women over 20 years of age were selected for this study who visited a health promotion center from January, 2008 to July, 2009. Patients with HBs Ag (+) or anti-HCV Ab (+) and those currently on medication that could affect hepatic function were excluded. The subjects' history of illness, their present health and medication status were examined through a doctor's history taking. Also, basic physical examinations and laboratory tests were performed on these subjects. Results: Classifying the serum γ-GT into quartiles, as the γ-GT classification stage increased, there was an increase in age, alcohol drinking, body mass index, systolic and diastolic blood pressure, total cholesterol, low density lipoprotein, triglyceride, fasting glucose, homocystein, high sensitivity C-reactive protein.(P for trend<0.05) When odds ratio(95% CI) for hypertension in the highest quartile of γ-GT was compared to that of the lowest quartile, there was an increased risk of hypertension with an odds ratio of 2.7(95% CI, 1.1∼6.2). Conclusion: This study showed that the risk of hypertension was increased according to serum γ-GT, even though the variable index affecting cardiovascular diseases was adjusted.

자발성 소뇌출혈에서 뇌전선화 단층촬영 소견이 초기의식 상태와 예후에 미치는 영향

이승희,정남,권창영,은종필 예수병원 1997 예수병원학술지 Vol.18 No.-

폐경 후 여성의 대퇴골 골밀도에 따른 대사증후군과의 관련성

임호섭,공은희 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

low bone mineral density (BMD). The aim of this study was to identify the relationship with metabolic syndrome by BMD of femur in postmenopausal women. Methods: We evaluated 120 postmenopausal women who were visiteda health promotion center in a university hospital from January, 2008 to December, 2009. Data on their current medical diseases and medications were collected through a physician's history taking. Also, basic physical examinations and laboratory tests were performed on these subjects. Results: In adjusted analysis including age and other factors, only waist circumference had a close correlation with BMD of femur (P< 0.05). The BMD of femur had no correlation with other risk factors of metabolic syndrome. When stratified by BMD of femur, corrected BMD of femur revealed no significant correlation with the presence of metabolic syndrome. Conclusion: The BMD of femur among postmenopausal women with metabolic syndrome shows a positive tendency in abdominal obesity

입원 환자 영양검색 전산 프로그램 개발

안수미,윤기영,신연명,최경현,임근승,장문경,송윤미,공은희 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

Background : About 30% of patients in the hospital are under nourishment. A large portion of people are undernourished when they are admitted to the hospital and in the majority of these, undernutrition develops further while in hospital. Patient-Generated subjective global assessment(PG-SGA), a method of nutritional assessment based on clinical judgment, has been widely used to assess the nutritional status of adults for both clinical and research purposes. Modified PG-SGA can be used as a nutrition screening tool, and a comprehensive nutrition assessment is desirable for those who are malnourished. The purpose of this study is to develop a simple, reliable and valid nutrition screening program(NSP). Methods : We applied the nutrition screening program(NSP) to adult inpatients who are at risk of malnutrition using the Modified Patient-Generated subjective global assessment(PG-SGA) and objective assessment(electrical medical record data). Results : Therefore simple and beneficial NSP is developed for patients with severe malnutrition. Using NSP. nutritional information of the severe malnutrition patient should be shared with the physicians and they should be taken care of by clinical dietitians to improve their nutritional status. Conclusion : The Modified PG-SGA and nutrition screening tool are simple, reliable and valid nutrition screening program(NSP).

The expression and clinical implication of GAGE gene in Stomach Cancer

Kong, Eun-Hee,Chang, Hee-Kyung 고신대학교 의학부 2005 高神大學校 醫學部 論文集 Vol.20 No.1

배경 : 자가 T 임파구에 의해 인식된 암-특정 유전자 발현을 제외하고 위암에서 GAGE 유전자의 기능에 대한 정보가 아직도 거의 없다. 이 연구는 위암에서 임상적 암시와 암조직발생에서의 기능적인 역할을 밝히기 위해 GAGE mRNA 발현을 평가하기 위한 시도이다. 방법 : 위암 환자 60명으로부터 종양과 비종양 조직을 둘씩 짝을 지어 추출하여 common primer를 가지고 reverse transcription-nested polymerase chain reaction(RT-PCR)을 이용하여 연구하였다. 결과 : 비종양조직에서는 GAGE 발현이 없었다. 60개의 종양 조직으로부터 15개는 GAGE mRNA 발현(25.0%), 13개는 intestinal type(86.7%), 2개만 diffuse type(13.3%)을 나타냈다. 암 조직에서 GAGE 발현은 높은 암의 단계와 림프절 전이에 중대한 영향을 가진다(p<0.05). 그러나 그들은 종양 세포 분화와 혈관, 신경 주위 침범과는 중대한 관계를 보여 주지 않았다. 결론 : GAGE 유전자가 위암의 intestinal type 발생과 진행에 중요한 역할을 하고 GAGE 유전자가 암-특정 면역요법의 유용한 목표 분자일지도 모른다는 것을 암시한다. Background: There is still little information on the function of GAGE gene in the stomach cancer except for cancer-specific gene expression recognized by autologous T lymphocytes. This study attempted to evaluare GAGE mRNA expression to elucidate its functional role in the carcinohistogenesis and clinical implication in gastric cancer. Material and Method: Tumor and non-neoplastic paired samples from 60 patients with gastric cancer were studied by using reverse transcription - nested polymerase chain reaction (RT-PCR) with common primer. Result: No expression of GAGE was observed in non-neoplastic tissues. Fifteen out of 60 tumor tissues expressed GAGE (25.0%) mRNA, of which 13 cases (86.7%) were intestinal type and only 2 cases (13.3%) were diffuse type. GAGE expressions in cancer tissues have a significant tendency to be higher by stage and lymph node metatastasis (p<0.05). However, they did not show significant relationship with tumor cell differentiation and vascular and perineural invasions. Conclusions: This results suggest that GAGE gene might have an important role in the development and progession of intestinal type of stomach cancer and GAGE gene may be a useful molecule for target of cancer-specific immunotherapy.

Molecular characterization and expression of the Macrophage migration inhibitory factor gene from the pacific abalone (Haliotis discus hannai)

Eun Hee Park,Eun-Ha Shin,Young-Ok Kim,Dong-Gyun Kim,Hee Jeong Kong,Woo-Jin Kim,Jung Youn Park,Bo-Hye Nam 한국수산과학회 양식분과 2017 한국수산과학회 양식분과 학술대회 Vol.2017 No.5