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患者로부터 分離同定한 病原性 大腸菌의 性狀에 관한 硏究
염곤,劉承兆,李善煥 成均館大學校 科學技術硏究所 1984 論文集 Vol.35 No.1
Strains of Pathogenic Escherichia coli isolated from the diarrheal patients in the Seoul were 9 serotypes that were documented previously in the international literature as associated with Pathogenic E. coli. Of 13 strains, there are 9 serotypes and 3 strains are Enterotoxigenic E. coli. The serotypes of Enterotoxigenic E. coli were 06 : K15, 0148 : K, 025 : Kl. In order to detect the heat-stable enterotoxin of Enterotoxigenic E. coli, supernants of CA-YE culture were administered into the intestines of suckling mice and fluid accumulation in the intestine were measured after 4 hrs., by weighing. In the results of suckling mice test, it have proved that 2 strains (06 : K15, 00148 : K) had produced positive tests. In the results of the antibiotics susceptibility test, Nitrofurantion and Nalidixic acid about the urinary tract infection have susceptibility but Tetracyclines such as Doxycycline, Oxytetracycline have high resistance. Although Neomycin had been treated skin or wounds, generally it had resistance because we suggest that it have cross-resistance with kanamycin which is similar activity and widely treated the disease in the pathogenic E. coli.
염곤,劉承兆,李長勳 成均館大學校 科學技術硏究所 1985 論文集 Vol.36 No.1
The pathogenesis of gastrointestinal infections caused by E. coli has been attributed to the ablility of some strains to produce enterotoxins or to invade the intestinal mucosa directly. Enterotoxigenic strains may produce either a heat-labile toxin(LT), a heat-stable toxin(ST) or both LT and ST. The heat-labile enterotoxin of E. coli causes a diarrhea with a delayed onset and similalar to cholera toxin in its mode of action and immunogenicity. In contrast to LT, ST is of low molecular weight, nonimmunogenic, and not neutralized by cholera or LT antitoxin. The infant mouse test was used for the detection of ST and EnzymeL-inked Immunosorbent Assay was used to dettect LT, and serological test was used to detect Enteropathogenic E. coli and Enteroinva sive E. coli. And biochemical examinations were done. 1. Strains of pathogenic E. coli isolated from the diarrheal patients in Seoul were identifided in 68 (21.3%) of 319 cases. 2. Stranis of enterotoxigenic E. coli was detcetected in 56 cases; 49 were positive for heat-labile enterotoxin and 1 was positive for only the heat-stable enterotoxin. 3. Strains of enteropathogenic E. coli was detected in 10 cases. 4. The serotypes of enteropathogenic E. coli were 026 : K60, 055 : K59, 0111 : K58, 0119 : K69, 0126 K71, 0128 : K67, 01 : K51. 5. D-mannitol, lactose, maltose were used for growth in toxigenic E. coli. Malonate utilization, urease activity, gelatin liquefaction were negetive in enteropathogenic E. coli, enterotoxigenic E. coli and enteroinvasive E. coli.
염곤,최병돈,Ryeom, Kon,Choi, Byung-Don 한국생약학회 2000 생약학회지 Vol.31 No.1
These studies were designed to determine the potential cytotoxic activity of MeOH extracts of 65 crude drugs against leukemia L1210 and $P388D_1$, cell line in vitro, of which 25 samples were selected. The n-BuOH extracts of 25 samples were measured using the same method and nine were selected. These samples were measured for the potential antitumor activity against $P388D_1$, for life span in vivo, and against sarcoma 180 for tumor weight. On the basis of results, Notoginseng Radix, Anemarrhenae Rhizoma, Albizziae Cortex, Portulacae Herba, Bupleuri Radix were evaluated the effective plant on the antitumor activity. In addition, the mixture of Notoginseng Radix and Albizziae Cortex was evaluated to enhance the antitumor activity in vivo.
서울에서 分離된 腸內細菌의 抗生劑耐性과 耐性傳達因子에 관한 硏究
劉承兆,金勝喜,염곤 成均館大學校 1980 論文集 Vol.28 No.-
Studies on drug resistance and R-factor of enteric bacterial flora isolated from Seoul area(June 1978∼Sept. 1978). Drug Resistance and the distribution of R-factor were examined using Escherichia coli, Salmonella, Shigella, Enterobacter and Klebsiella isolated from patients in Seoul area from June 1978 through Sept. 1978. 1. Of Escherichia coli, 147 strains (87.50%) were resistant to Ampicillin, but 2 stranins (1.19%) only were resistant to Nalidixic acid. Of Shigella, no strains were resistant to Gentamicin, and the persectage of resistancd to Streptomycin was highest (26.53%). Of Salmonella, no strains were resistant to Nalidixic acid, Tetracyclin, Gentamicin, Chloramphenicol, but the percentage of resistance to Streptomycin was high (95.04%). Of Enterobacter, 100% were tesistant to Amplicillin and Tetracyclin and of Klebsiella, 100% were resistant to Ampicillin. 2. Of Escherichia coli, most of strains were multiply resistant, while only 2 strains were singly resistant. Of Salmonella, 88.14% of resistant strains were singly resistant and of Enterobacter, all of 29 resistant strains were multiply resistant. 3. Escherichia coli, transferred R-factor in 81.76%, Shigella 25.00%, Klebsiella 93.48%, Enterobacter 86.96%, while Salmonella didn't transfer R-factor at all. 4. 23 resistant patterns of R-factor were found ifn Escherichia coli, 4 resistant patterns in Shigella, 7 resistant patterns in Klebsiella, and 3 resistant patterns in Enterobacter, especially, a resistant pattern of R-factor (AP)^r was 54.62% in Escherichia coli, 57.1% in Shigella, and 35.00% in Enterobacter, which was the highest one of resistent patterns of R-factor, while (AP, TC)^r was 48.84% in Klebsiella. 5. According to the result of examination, the multiply resistant strains transferred R- factor more easily than the singly resistant strains.
한국 Streptomyces sp. 로 부터 분리한 방향족 화합물과 지질 화합물의 세포독성 연구
신석우(Suck Woo Shin),염곤(Kon Ryeom) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.2
In an effort to screen new selective antitumor agents from the broth of soil microorganism, cytotoxicity oriented screening was performed against tumor cells and 3 compounds (Compound 1, 2 and 3) were isolated from Streptomyces parvullus ISP 5048 and their chemical structures were determined. Among these compounds, Compound 2 showed the highest cytotoxicity against P388D1 and L1210. While the IC_(50) values of compound 2 against P388D1 and L1210 were 0.073 ㎍/㎖ and 0.07 ㎍/㎖, respectively, and the IC_(50) value of Compound 3 was 0.17 ㎍/㎖ against human lung cancer cells, A549, the cytotoxicity of Compound 2 and 3 against normal cell line, Vero E6 cell was about 4- and 8-fold lower than that of adriamycin. Based on the chemical analysis data, Compound 3 was octacosamicine A, a known antibiotic, which was reported by Dobasih et al. (1988). Taken together the results demonstrated that Compound 2 and Compound 3 has the possibility to be developed as antitumor agent because of its potent cytotoxicity as well as high selectivity against various cancer cell lines.
최병돈,김승철,염곤 단국대학교 대학원 1992 學術論叢 Vol.16 No.-
A wild strain was isolated through screening method from soil sample and identified by ISP system. This strain was shown to be similar to Streptomyces noboritoensis. The antibiotic which produced by wild strain showed only activity against the gram(+) bacteria. The antibiotic was purified by butanol extraction, column chromatography and paper chromatography. This antibiotic was soluble in alcohols and was colored by ferric chloride, anisaldehyde-sulfuric acid, acetic anhydride-sulfuric acid and antimony(Ⅲ) chloride. The chemical structure through color reaction. UV spectrum, IR spectrum, mass spectrum was assumed as a terpene being alcoholic hydroxyl group and the molecular weight was 211.