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( Jeongmin Lee ),( Byoung Woo Lee ),( Hae-eun Kang ),( Kevine K. Choe ),( Moosik Kwon ),( Chongsuk Ryou ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.5
The conformational change of cellular prion protein (PrP<sup>C</sup>) to its misfolded counterpart, termed PrP<sup>Sc</sup>, is mediated by a hypothesized cellular cofactor. This cofactor is believed to interact directly with certain amino acid residues of PrP<sup>C</sup>. When these are mutated into cationic amino acid residues, PrPSc formation and prion replication halt in a dominant negative (DN) manner, presumably due to strong binding of the cofactor to mutated PrP<sup>C</sup>, designated as DN PrP mutants. Previous studies demonstrated that plasminogen and its kringle domains bind to PrP and accelerate PrP<sup>Sc</sup> generation. In this study, in vitro binding analysis of kringle domains of plasminogen to Q167R DN mutant PrP (PrPQ167R) was performed in parallel with the wild type (WT) and Q218K DN mutant PrP (PrPQ218K). The binding affinity of PrPQ167R was higher than that of WT PrP, but lower than that of PrPQ218K. Scatchard analysis further indicated that, like PrPQ218K and WT PrP, PrPQ167R interaction with plasminogen occurred at multiple sites, suggesting cooperativity in this interaction. Competitive binding analysis using L-lysine or L-arginine confirmed the increase of the specificity and binding affinity of the interaction as PrP acquired DN mutations. Circular dichroism spectroscopy demonstrated that the recombinant PrPs used in this study retained the α-helix-rich structure. The α-helix unfolding study revealed similar conformational stability for WT and DN-mutated PrPs. This study provides an additional piece of biochemical evidence concerning the interaction of plasminogen with DN mutant PrPs.
Cedric, Chimi Djomo,Nfornkah, Barnabas Neba,Louis-Paul-Roger, Kabelong Banoho,Kevine, Tsoupoh Kemnang Mikelle,Awazi, Nyong Princely,Forje, Gadinga Walter,Louis, Zapfack Institute of Forest Science 2022 Journal of Forest Science Vol.38 No.3
Triplochiton scleroxylon K. Schum is the plant species most affected by logging activities in the East Region of Cameroon due to its market value. This logging has impacted the ecological niche of the fern plant for which limited research has been done. The aim of this study is to contribute towards improving knowledge of fern richness and biomass on T. scleroxylon within the Central African sub-region. Fern data collection was done on 20 felled/harvested T. scleroxylon where, in addition to fern inventory, fern biomass was collected by the destructive method. The diameter and height of T. scleroxylon measured were used as explanatory variables in allometric equations for fern biomass estimation. Fern inventory was characterized using diversity index. Eight fern species were recorded on T. scleroxylon (≈5 species/T. scleroxylon). The minimum diameter where fern could be found is 59.4 cm. The average fern biomass found was 23.62 kg/T. scleroxylon. Pearson correlation coefficient showed a positive correlation (r>0.55) between fern biomass and T. scleroxylon diameter. For allometric equation, the logarithmic model improved better the adjustment than the non-logarithmic model. However, the quality of the adjustment is improved more when only the diameter is considered as an explanatory variable. Fern biomass is estimated to 90.08 kg/ha<sup>-1</sup> with 76.02 kg/ha<sup>-1</sup> being lost due to T. scleroxylon exploitation in the study area. This study is a contribution towards increasing knowledge of fern diversity specific to T. scleroxylon, and also fern biomass contribution to climate change mitigation and the potential carbon loss due to T. scleroxylon exploitation.
Su Shiwei,Tan Donghong,Li Xianghua,Li Xin,Chen Zitong,Lian Ruiqing,Lenz Soronga Blaise Kevine 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.2
The high proportion of grid-connected intermittent energy such as wind power and photovoltaic makes the demand for power system flexibility increase sharply. In order to improve the overall flexibility of new energy high permeability system and enhance the economy of power system, promote the new energy consumption of system. This paper presents a multi-time scale Coordinated optimization strategy for the new energy high permeability power system considering flexibility requirement (MTSCOSFR). This strategy makes full use of the adjustment ability of source-load-storage flexible resources and analyzes system flexibility requirements at different time scales, creates multi-time scale optimization scheduling models, including typical week operation simulation model, typical day operation simulation model, typical period operation simulation model. Puts the profit of carbon trading and the penalty cost of insufficient flexibility into the optimization goal. Adopts the idea of multi-time coordination and step-by-step optimization to solve the model. This paper sets two cases to discuss MTSCOSFR. The results show that the comprehensive cost of the typical weekly operation simulation is reduced to 2360 × 104$, the penalty cost of insufficient flexibility in the typical day operation simulation is reduced by 67%, the flexibility capacity increased by 500 MW, the regulation capacity increased by 300 MW, the ramp margin increased by 1500 MW/(5 min) −1. The proposed strategy can improve system flexibility and promote new energy consumption on the basis of ensuring system economy.
Kevin Moncion(Kevin Moncion ),Letizia Gardin(Letizia Gardin ),Jane Lougheed(Jane Lougheed ),Kristi Adamo(Kristi Adamo ),Patricia E. Longmuir(Patricia E. Longmuir ) 사피엔시아 2022 Exercise Medicine Vol.6 No.-
Objectives: This exploratory mixed-methods study explored the barriers to physical activity, daily physical activity and submaximal exercise capacity among children with and at risk for cardiomyopathy and children with atrial septal defects. Methods: The study followed a convergent parallel mixed methodology design. Semi-structured interviews explored physical activity barriers. Seven-day accelerometry assessed moderate-to-vigorous physical activity, and an intermittent cardiopulmonary exercise test measured submaximal exercise capacity. Results: Twenty children, including 5 with cardiomyopathy (n=2 females, 14.2 ± 2.7 years old), 7 who were genotype-positive phenotype-negative for cardiomyopathy (n=5 females, 10.6 ± 3.3 years old) and 8 with atrial septal defects (n=4 females, 9.4 ± 3.8 years old) were recruited. Children with cardiomyopathy reported disease-specific physical activity barriers, while children who were genotype-positive phenotype-negative perceived barriers related to lack of time, parent support or activity motivation. The average daily moderate-to-vigorous physical activity was less than the recommended 60-minutes/day (n=20, mean 48.1 ± 18.0 minutes). Children with cardiomyopathy participated a median of 141.2 [interquartile range (IQR): 98.8) minutes of light-intensity physical activity and a median of 55.6 (IQR: 34.6) minutes of moderate-to-vigorous physical activity. The average submaximal exercise capacity was low (n=16, 25.2 ± 5.7 mL/kg/min). Estimated submaximal exercise capacity, including metabolic equivalent (4.5 ± 3.1 METs), respiratory exchange ratio (median = 1.0, IQR: 0.09) and ratings of perceived exertion (median = 7, IQR: 5) at peak exercise suggest that children with cardiomyopathy appear to have the exercise capacity to participate in low-to-moderate intensity activities. Conclusions: These novel data suggest that a diagnosis of cardiomyopathy may not preclude children from participating in a healthy, active lifestyle. However, they perceive disease-specific physical activity barriers and may require support to optimize their level of participation for optimal health.
Lexical Bundles in the Argumentative Essays of Vietnamese and American Student Writers
Kevin Parent(Kevin Parent ) 한국영어어문교육학회 2024 영어어문교육 Vol.30 No.1
This study examines lexical bundles used in argumentative writings by Vietnamese EFL university students-L2 learners and American university students-L1 learners to see how two groups use bundles and identify problems which L2 learners face. Two corpora including writings by L2 and L1 learners are collected. By using Biber et al.’s structural taxonomy (1999, 2004) and Hyland’s functional framework (2008a), we analyse the structures and functions of the four-word bundles used by the two group writings to see any differences. Compared with L1 learners, L2 learners use a wider range of bundles. Regarding structures, L2 learners overuse VP-based bundles, while L1 learners use VP, NP and PP-based bundles flexibly. For functions, L1 learners prefer to use participant-oriented bundles, while L2 learners tend to employ researchoriented bundles. The text-oriented bundles are the least commonly occurring ones in the two groups’ essays. To understand how L2 learners overuse bundles in their writings, a qualitative method is used to examine each essay. Some underlying explanations for the overuse might be side effects of teaching and lack of knowledge of the bundle. A pedagogical implication of teaching L2 learners to use bundles in writing class is suggested to help their writing more convincingly and academically.
Kevin LOE(Kevin LOE ),Aniq TASIA(Aniq TASIA ),Ricardo INDRA(Ricardo INDRA ),La MANI(La MANI ) 한국유통과학회 2022 유통과학연구 Vol.20 No.9
Purpose: This study analyzes the effects of variables affecting e-satisfaction and e-loyalty in e-commerce. Research design, data, and methodology: A survey was conducted to 384 Indonesia online shopping mobile application users. The questionnaire construct was designed based on several independent variables, such as application attractiveness, application functionality, and customer service on e-satisfaction and e-loyalty. E-satisfaction was positioned as an intervening variable to determine the effect on e-loyalty. Results: Application attractiveness, application functionality, and customer service shown positive effect on e-satisfaction in online shopping mobile application. At the same time, e-satisfaction also affected e-loyalty significantly as referred in previous studies. However, application attractiveness, while showed positive effect on e-satisfaction, in contrary with previous study does not showed very significant effect. Conclusion: Application functionality significantly influences users satisfaction compared to application attractiveness. E-satisfaction mediates as a factor between visual design, information access, and transaction in online shopping mobile application, which stimulates user loyalty. The study findings’ can be a reference for companies to create and maintain users’ satisfaction and loyalty via different aspects of mobile application. Further research should be conducted on other field of study as the industry has different aspects that affects user loyalty.