Metabolic Profiling and Biological Activities of Bioactive Compounds Produced by Pseudomonas sp. Strain ICTB-745 Isolated from Ladakh, India

( Kamal ),( Ahmed ),( Anver Basha Shaik ),( C. Ganesh Kumar ),( Poornima Mongolla ),( P. Usha Rani ),( K. V. S ),( Rama Krishna ),( Suman Kumar Mamidyala ),( Joveeta Joseph ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1

In an ongoing survey of the bioactive potential of microorganisms from Ladakh, India, the culture medium of a bacterial strain of a new Pseudomonas sp., strain ICTB-745, isolated from an alkaline soil sample collected from Leh, Ladakh, India, was found to contain metabolites that exhibited broad-spectrum antimicrobial and biosurfactant activities. Bioactivity-guided purification resulted in the isolation of four bioactive compounds. Their chemical structures were elucidated by 1H and 13C NMR, 2D-NMR (HMBC, HSQC, 1H,1H-COSY, and DEPT- 135), FT-IR, and mass spectroscopic methods, and were identified as 1-hydroxyphenazine, phenazine-1-carboxylic acid (PCA), rhamnolipid-1 (RL-1), and rhamnolipid-2 (RL-2). These metabolites exhibited various biological activities like antimicrobial and efficient cytotoxic potencies against different human tumor cell lines such as HeLa, HepG2, A549, and MDA MB 231. RL-1 and RL-2 exhibited a dose-dependent antifeedant activity against Spodoptera litura, producing about 82.06% and 73.66% antifeedant activity, whereas PCA showed a moderate antifeedant activity (63.67%) at 60 μg/cm2 area of castor leaf. Furthermore, PCA, RL-1, and RL-2 exhibited about 65%, 52%, and 47% mortality, respectively, against Rhyzopertha dominica at 20 μg/ml. This is the first report of rhamnolipids as antifeedant metabolites against Spodoptera litura and as insecticidal metabolites against Rhyzopertha dominica. The metabolites from Pseudomonas sp. strain ICTB-745 have interesting potential for use as a biopesticide in pest control programs.

Nominal axial and flexural strengths of high-strength concrete columns

Mustafa Kamal Al-Kamal 사단법인 한국계산역학회 2019 Computers and Concrete, An International Journal Vol.24 No.1

The ACI building code is allowing for higher strength reinforcement and concrete compressive strengths. The nominal strength of high-strength concrete columns is over predicted by the current ACI 318 rectangular stress block and is increasingly unconservative as higher strength materials are used. Calibration of a rectangular stress block to address this condition leads to increased computational complexity. A triangular stress block, derived from the general shape of the stressstrain curve for high-strength concrete, provides a superior solution. The nominal flexural and axial strengths of 150 highstrength concrete columns tests are calculated using the proposed stress distribution and compared with the predicted strength using various design codes and proposals of other researchers. The proposed triangular stress model provides similar level of accuracy and conservativeness and is easily incorporated into current codes.

Nominal flexural strength of high-strength concrete beams

Al-Kamal, Mustafa Kamal Techno-Press 2019 Advances in concrete construction Vol.7 No.1

The conventional ACI rectangular stress block is developed on the basis of normal-strength concrete column tests and it is still being used for the design of high-strength concrete members. Many research papers found in the literature indicate that the nominal strength of high-strength concrete members appears to be over-predicted by the ACI rectangular stress block. This is especially true for HSC columns. The general shape of the stress-strain curve of high-strength concrete becomes more likely as a triangle. A triangular stress block is, therefore, introduced in this paper. The proposed stress block is verified using a database which consists of 52 tested singly reinforced high-strength concrete beams having concrete strength above 55 MPa (8,000 psi). In addition, the proposed model is compared with models of various design codes and proposals of researchers found in the literature. The nominal flexural strengths computed using the proposed stress block are in a good agreement with the tested data as well as with that obtained from design codes models and proposals of researchers.

A2E "water purifier"

Kamal Pandit,Kamal Shah 한국디자인학회 2014 한국디자인학회 국제초대전도록 Vol.2014 No.5

Comprehensive proteome analysis using quantitative proteomic technologies

Kamal, Abu Hena Mostafa,Choi, Jong-Soon,Cho, Yong-Gu,Kim, Hong-Sig,Song, Beom-Heon,Lee, Chul-Won,Woo, Sun-Hee The Korean Society of Plant Biotechnology 2010 식물생명공학회지 Vol.37 No.2

With the completion of genome sequencing of several organisms, attention has been focused to determine the function and functional network of proteins by proteome analysis. The recent techniques of proteomics have been advanced quickly so that the high-throughput and systematic analyses of cellular proteins are enabled in combination with bioinformatics tools. Furthermore, the development of proteomic techniques helps to elucidate the functions of proteins under stress or diseased condition, resulting in the discovery of biomarkers responsible for the biological stimuli. Ultimate goal of proteomics orients toward the entire proteome of life, subcellular localization, biochemical activities, and their regulation. Comprehensive analysis strategies of proteomics can be classified as three categories: (i) protein separation by 2-dimensional gel electrophoresis (2-DE) or liquid chromatography (LC), (ii) protein identification by either Edman sequencing or mass spectrometry (MS), and (iii) quanitation of proteome. Currently MS-based proteomics turns shiftly from qualitative proteome analysis by 2-DE or 2D-LC coupled with off-line matrix assisted laser desorption ionization (MALDI) and on-line electrospray ionization (ESI) MS, respectively, to quantitative proteome analysis. Some new techniques which include top-down mass spectrometry and tandem affinity purification have emerged. The in vitro quantitative proteomic techniques include differential gel electrophoresis with fluorescence dyes, protein-labeling tagging with isotope-coded affinity tag, and peptide-labeling tagging with isobaric tags for relative and absolute quantitation. In addition, stable isotope labeled amino acid can be in vivo labeled into live culture cells through metabolic incorporation. MS-based proteomics extends to detect the phosphopeptide mapping of biologically crucial protein known as one of post-translational modification. These complementary proteomic techniques contribute to not only the understanding of basic biological function but also the application to the applied sciences for industry.

Influence of the Recombination Parameters at the Si/SiO₂ Interface on the Ideality of the Dark Current of High Efficiency Silicon Solar Cells

Kamal, Husain,Ghannam, Moustafa The Institute of Electronics and Information Engin 2015 Journal of semiconductor technology and science Vol.15 No.2

Analytical study of surface recombination at the $Si/SiO_2$ interface is carried out in order to set the optimum surface conditions that result in minimum dark base current and maximum open circuit voltage in silicon solar cells. Recombination centers are assumed to form a continuum rather than to be at a single energy level in the energy gap. It is shown that the presence of a hump in the dark I-V characteristics of high efficiency PERL cells is due to the dark current transition from a high surface recombination regime at low voltage to a low surface recombination regime at high voltage. Successful fitting of reported dark I-V characteristics of a typical PERL cell is obtained with several possible combinations of surface parameters including equal electron and hole capture cross sections.

Effect of Acibenzolar-S-methyl and Rahnella aquatilis (Ra39) on Chitinase and β-1, 3-glucanase Activities and Disease Resistance of Apple Plants

Kamal A. M. Abo-Elyousr,M. A. A. Sallam,M. H. A. Hassan,W. Zeller 한국식물병리학회 2010 Plant Pathology Journal Vol.26 No.1

The effect of Acibenzolar-S-methyl (ASM) and Rahnella aquatilis Ra39 against apple fire blight disease caused by Erwinia amylovora were tested as a possible alternative to streptomycin. In vitro studies, no inhibition effect against the pathogen was found when ASM was tested. Under greenhouse conditions, application of R. aquatilis Ra39 with the highly susceptible M26 rootstock resulted in a marked disease suppression. Application of ASM and strain Ra39 caused a high decrease of the disease, 82% and 58% respectively; this was correlated with a reduction of the growth of the pathogen within host plants up to 64% and 49.5% respectively. Further studies in the field under artificial infection condition during full bloom revealed that application of ASM and R. aquatilis Ra39 with Gala variety resulted in a control effect up to 21 and 29% respectively. In physiological studies, enhanced activities of PR-proteins (chitinase and β-1, 3-glucanase) were detected, which are well known as biochemical markers for systemic acquired resistance. Application of ASM to apple shoots caused the highest chitinase activity followed by strain Ra39. The enzyme activity was increased after 2, 4 and 6 days from application. In addition, ASM-treatment caused the higher β-1, 3-glucanase activity than strain Ra39. Maximum enzyme activity was recorded after 6 days from application and then decreased after 8 and 10 days from application.

Genetic Diversity of New Maize Hybrids Based on SSR Markers as Compared with Other Molecular and Biochemical Markers

Kamal Foud Abdellatif,Yehia Abdallah Khidr 한국작물학회 2010 Journal of crop science and biotechnology Vol.13 No.3

Genetic diversity of four new yellow single crosses, five new yellow three-way crosses, and five yellow inbred lines of maize (Zea mays L.) was studied using different molecular (SSR, ISSR, and RAPD) and biochemical markers (seed storage protein content). All markers were able to clearly separate the inbred lines in one cluster from the different types of hybrids. The correlation among the different types of molecular markers was moderately high according to the Mantel's test (e.g. 0.67 between SSR and ISSR, 0.42between SSR and RAPD, and 0.65 between ISSR and RAPD), indicating that the three techniques are efficient for evaluating genetic diversity in the maize genotypes. The correlation of biochemical markers (seed storage protein SDS-PAGE) with SSR, ISSR, and RAPD markers was 0.61, 0.74, and 0.48, respectively. It can be concluded that both molecular and biochemical markers are efficient to study the genetic diversity in maize. Among the different types of molecular markers, SSR is a more accurate marker-type because of its co-dominance and stability of results. It can also be said that biochemical and molecular markers are positively correlated and the correlation ranged between moderate to high. This could suggest using both marker types, separately or together, for genetic diversity studies in maize.

Evaluation and Identification of Promising Bivoltine Hybrids of Silkworm, Bombyx mori L., for Monsoon and Spring Season of Uttar Pradesh

( Kamal Jaiswal ),( Rachna Goel ) 한국잠사학회 2003 International Journal of Industrial Entomology Vol.6 No.2

Enhanced Onion Resistance against Stemphylium Leaf Blight Disease, Caused by Stemphylium vesicarium, by Di-potassium Phosphate and Benzothiadiazole Treatments

Kamal, Abo-Elyousr A.M.,Mohamed, Hussein M.A.,Aly, Allam A.D.,Mohamed, Hassan A.H. The Korean Society of Plant Pathology 2008 Plant Pathology Journal Vol.24 No.2

In this study, we investigated the induced defense response and protective effects against Stemphylium vesicarium by application of benzothiadiazole ($Bion^{(R)}$) and di-potassium phosphate salt $(K_2HPO_4)$ to onion. Onion leaves were sprayed with $Bion^{(R)}$ and $K_2HPO_4$, then inoculated 2 days later with a virulent strain of S. vesicarium under greenhouse conditions. Disease severity and activities of peroxidase (PO), polyphenoloxidase, phenylalanine ammonia-lyase (PAL) and phenol contents were evaluated in the treated leaf tissues. Reduction in the disease severity was observed in plants treated with $Bion^{(R)}$ and $K_2HPO_4$. Onion plants treated with $Bion^{(R)}$ and $K_2HPO_4$ and inoculated with the pathogen showed significantly higher PAL activity, PO activity, and phenol contents than inoculated water-treated plants 2 days after the treatment. In conclusion, the results of this study provide evidence that application of simple non-toxic chemical solutions as di-potassium phosphate and $Bion^{(R)}$ can control Stemphylium leaf blight of onion.